Microarc oxidation surface of titanium implants promote osteogenic differentiation by activating ERK1/2-miR-1827-Osterix

2020 ◽  
Vol 56 (4) ◽  
pp. 296-306
Author(s):  
Liu Liu ◽  
Da Zeng ◽  
Yanwen Chen ◽  
Junbo Zhou ◽  
Yunyang Liao ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Oxidation Surface

scholarly journals Osteogenic Differentiation of Human Mesenchymal Stem Cells Modulated by Surface Manganese Chemistry in SLA Titanium Implants

2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Jin-Woo Park ◽  
Yusuke Tsutsumi ◽  
Eui-Kyun Park
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Corrosion Resistance ◽  
Osteogenic Differentiation ◽  
Chemical Treatment ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Potential Candidate ◽  
Implant Surface ◽  
Wet Chemical

The manganese (Mn) ion has recently been probed as a potential candidate element for the surface chemistry modification of titanium (Ti) implants in order to develop a more osteogenic surface with the expectation of taking advantage of its strong binding affinity to the integrins on bone-forming cells. However, the exact mechanism of how Mn enhances osteogenesis when introduced into the surface of Ti implants is not clearly understood. This study investigated the corrosion resistance and potential osteogenic capacity of a Mn-incorporated Ti surface as determined by electrochemical measurement and examining the behaviors of human mesenchymal stem cells (MSCs) in a clinically available sandblasted/acid-etched (SLA) oral implant surface intended for future biomedical applications. The surface that resulted from wet chemical treatment exhibited the formation of a Mn-containing nanostructured TiO2 anatase thin film in the SLA implant and improved corrosion resistance. The Mn-incorporated SLA surface displayed sustained Mn ion release and enhanced osteogenesis-related MSC function, which enhanced early cellular events such as spreading, focal adhesion, and mRNA expression of critical adhesion-related genes and promoted full human MSC differentiation into mature osteoblasts. Our findings indicate that surface Mn modification by wet chemical treatment is an effective approach to produce a Ti implant surface with increased osteogenic capacity through the promotion of the osteogenic differentiation of MSCs. The improved corrosion resistance of the resultant surface is yet another important benefit of being able to provide favorable osseointegration interface stability with an increased barrier effect.

scholarly journals AntibacterialTiO2Coating Incorporating Silver Nanoparticles by Microarc Oxidation and Ion Implantation

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Zhang ◽  
Zhiguo Zhang ◽  
Wei Li
Keyword(s):  
Antibacterial Activity ◽  
Ion Implantation ◽  
Bacterial Colonization ◽  
Pure Titanium ◽  
Microarc Oxidation ◽  
Optimal Strategies ◽  
Titanium Implants ◽  
Silver Ion ◽  
Antibacterial Ability ◽  
Replacement Surgery

Infection associated with titanium implants remains the most common serious complication in hard tissue replacement surgery. Since such postoperative infections are usually difficult to cure, it is critical to find optimal strategies for preventing infections. In this study, TiO2coating incorporating silver (Ag) nanoparticles were fabricated on pure titanium by microarc oxidation and ion implantation. The antibacterial activity was evaluated by exposing the specimens toStaphylococcus aureusand comparing the reaction of the pathogens to Ti-MAO-Ag with Ti-MAO controls. Ti-MAO-Ag clearly inhibited bacterial colonization more than the control specimen. The coating’s antibacterial ability was enhanced by increasing the dose of silver ion implantation, and Ti-MAO-Ag20.0 had the best antibacterial ability. In addition, cytocompatibility was assessed by culturing cell colonies on the specimens. The cells grew well on both specimens. These findings indicate that surface modification by means of this process combining MAO and silver ion implantation is useful in providing antibacterial activity and exhibits cytocompatibility with titanium implants.

scholarly journals Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kaimin Wu ◽  
Mengyuan Liu ◽  
Nan Li ◽  
Li Zhang ◽  
Fanhui Meng ◽  
...  
Keyword(s):  
Sustained Release ◽  
Osteogenic Differentiation ◽  
Sodium Hyaluronate ◽  
Release Profile ◽  
Titanium Implants ◽  
Layer By Layer ◽  
Matrix Mineralization ◽  
In Vitro Transfection

Abstract Background The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. Methods Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. Results The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. Conclusions The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

Porous Coated Titanium Implants do Not Inhibit Mesenchimal Stem Cells Proliferation and Osteogenic Differentiation

2013 ◽  
Vol 27 (6) ◽  
pp. 4290-4293 ◽  
Author(s):  
Boris Antonov ◽  
Ivan Bochev ◽  
Milena Mourdjeva ◽  
Plamen Kinov ◽  
Lubomir Tzvetanov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Titanium Implants

Long-term antibacterial activity of a composite coating on titanium for dental implant application

2020 ◽  
Vol 35 (6) ◽  
pp. 643-654
Author(s):  
Yicheng Cheng ◽  
Shenglin Mei ◽  
Xiangwei Kong ◽  
Xianghui Liu ◽  
Bo Gao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Implants ◽  
Composite Coating ◽  
Porphyromonas Gingivalis ◽  
Microarc Oxidation ◽  
Polymerase Chain Reaction Analysis ◽  
Titanium Implants ◽  
Actinobacillus Actinomycetemcomitans ◽  
Burst Release

Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens ( Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.

scholarly journals Effect of Nanosheet Surface Structure of Titanium Alloys on Cell Differentiation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Satoshi Komasa ◽  
Tetsuji Kusumoto ◽  
Yoichiro Taguchi ◽  
Hiroshi Nishizaki ◽  
Tohru Sekino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Titanium Alloys ◽  
Osteogenic Differentiation ◽  
Cellular Response ◽  
Alveolar Bone ◽  
Bone Marrow Cells ◽  
Titanium Implants ◽  
Protective Oxide ◽  
Rat Bone ◽  
Marrow Cells

Titanium alloys are the most frequently used dental implants partly because of the protective oxide coating that spontaneously forms on their surface. We fabricated titania nanosheet (TNS) structures on titanium surfaces by NaOH treatment to improve bone differentiation on titanium alloy implants. The cellular response to TNSs on Ti6Al4V alloy was investigated, and the ability of the modified surfaces to affect osteogenic differentiation of rat bone marrow cells and increase the success rate of titanium implants was evaluated. The nanoscale network structures formed by alkali etching markedly enhanced the functions of cell adhesion and osteogenesis-related gene expression of rat bone marrow cells. Other cell behaviors, such as proliferation, alkaline phosphatase activity, osteocalcin deposition, and mineralization, were also markedly increased in TNS-modified Ti6Al4V. Our results suggest that titanium implants modified with nanostructures promote osteogenic differentiation, which may improve the biointegration of these implants into the alveolar bone.

scholarly journals Osteoblast Response to Copper-Doped Microporous Coatings on Titanium for Improved Bone Integration

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Lai-jie Wang ◽  
Xiao-hui Ni ◽  
Fei Zhang ◽  
Zhi Peng ◽  
Fu-xun Yu ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Biological Activity ◽  
Bone Formation ◽  
Titanium Alloys ◽  
Bone Tissue ◽  
Bone Growth ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Material Surface ◽  
Proliferation And Differentiation

AbstractDue to their excellent mechanical properties and good biocompatibility, titanium alloys have become a popular research topic in the field of medical metal implants. However, the surface of the titanium alloy does not exhibit biological activity, which may cause poor integration between the interface of the titanium implant and the interface of the bone tissue and subsequently may cause the implant to fall off. Therefore, surface biological inertness is one of the problems that titanium alloys must overcome to become an ideal orthopedic implant material. Surface modification can improve the biological properties of titanium, thereby enhancing its osseointegration effect. Copper is an essential trace element for the human body, can promote bone formation and plays an important role in maintaining the physiological structure and function of bone and bone growth and development. In this study, a microporous copper-titanium dioxide coating was prepared on the surface of titanium by microarc oxidation. Based on the evaluation of its surface characteristics, the adhesion, proliferation and differentiation of MC3T3-E1 cells were observed. A titanium rod was implanted into the rabbit femoral condyle, and the integration of the coating and bone tissue was evaluated. Our research results show that the microporous copper-titanium dioxide coating has a nearly three-dimensional porous structure, and copper is incorporated into the coating without changing the structure of the coating. In vitro experiments found that the coating can promote the adhesion, proliferation and differentiation of MC3T3-E1 cells. In vivo experiments further confirmed that the titanium copper-titanium dioxide microporous coating can promote the osseointegration of titanium implants. In conclusion, copper-titanium dioxide microporous coatings can be prepared by microarc oxidation, which can improve the biological activity and biocompatibility of titanium, promote new bone formation and demonstrate good osteoinductive properties. Therefore, the use of this coating in orthopedics has potential clinical application.

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