Structure, MC3T3-E1 Cell Response, and Osseointegration of Macroporous Titanium Implants Covered by a Bioactive Microarc Oxidation Coating with Microporous Structure

2014 ◽  
Vol 6 (7) ◽  
pp. 4797-4811 ◽  
Author(s):  
Rui Zhou ◽  
Daqing Wei ◽  
Su Cheng ◽  
Wei Feng ◽  
Qing Du ◽  
...  
Keyword(s):  
Cell Response ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Microporous Structure

scholarly journals AntibacterialTiO2Coating Incorporating Silver Nanoparticles by Microarc Oxidation and Ion Implantation

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Zhang ◽  
Zhiguo Zhang ◽  
Wei Li
Keyword(s):  
Antibacterial Activity ◽  
Ion Implantation ◽  
Bacterial Colonization ◽  
Pure Titanium ◽  
Microarc Oxidation ◽  
Optimal Strategies ◽  
Titanium Implants ◽  
Silver Ion ◽  
Antibacterial Ability ◽  
Replacement Surgery

Infection associated with titanium implants remains the most common serious complication in hard tissue replacement surgery. Since such postoperative infections are usually difficult to cure, it is critical to find optimal strategies for preventing infections. In this study, TiO2coating incorporating silver (Ag) nanoparticles were fabricated on pure titanium by microarc oxidation and ion implantation. The antibacterial activity was evaluated by exposing the specimens toStaphylococcus aureusand comparing the reaction of the pathogens to Ti-MAO-Ag with Ti-MAO controls. Ti-MAO-Ag clearly inhibited bacterial colonization more than the control specimen. The coating’s antibacterial ability was enhanced by increasing the dose of silver ion implantation, and Ti-MAO-Ag20.0 had the best antibacterial ability. In addition, cytocompatibility was assessed by culturing cell colonies on the specimens. The cells grew well on both specimens. These findings indicate that surface modification by means of this process combining MAO and silver ion implantation is useful in providing antibacterial activity and exhibits cytocompatibility with titanium implants.

Long-term antibacterial activity of a composite coating on titanium for dental implant application

2020 ◽  
Vol 35 (6) ◽  
pp. 643-654
Author(s):  
Yicheng Cheng ◽  
Shenglin Mei ◽  
Xiangwei Kong ◽  
Xianghui Liu ◽  
Bo Gao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Implants ◽  
Composite Coating ◽  
Porphyromonas Gingivalis ◽  
Microarc Oxidation ◽  
Polymerase Chain Reaction Analysis ◽  
Titanium Implants ◽  
Actinobacillus Actinomycetemcomitans ◽  
Burst Release

Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens ( Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.

scholarly journals Osteoblast Response to Copper-Doped Microporous Coatings on Titanium for Improved Bone Integration

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Lai-jie Wang ◽  
Xiao-hui Ni ◽  
Fei Zhang ◽  
Zhi Peng ◽  
Fu-xun Yu ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Biological Activity ◽  
Bone Formation ◽  
Titanium Alloys ◽  
Bone Tissue ◽  
Bone Growth ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Material Surface ◽  
Proliferation And Differentiation

AbstractDue to their excellent mechanical properties and good biocompatibility, titanium alloys have become a popular research topic in the field of medical metal implants. However, the surface of the titanium alloy does not exhibit biological activity, which may cause poor integration between the interface of the titanium implant and the interface of the bone tissue and subsequently may cause the implant to fall off. Therefore, surface biological inertness is one of the problems that titanium alloys must overcome to become an ideal orthopedic implant material. Surface modification can improve the biological properties of titanium, thereby enhancing its osseointegration effect. Copper is an essential trace element for the human body, can promote bone formation and plays an important role in maintaining the physiological structure and function of bone and bone growth and development. In this study, a microporous copper-titanium dioxide coating was prepared on the surface of titanium by microarc oxidation. Based on the evaluation of its surface characteristics, the adhesion, proliferation and differentiation of MC3T3-E1 cells were observed. A titanium rod was implanted into the rabbit femoral condyle, and the integration of the coating and bone tissue was evaluated. Our research results show that the microporous copper-titanium dioxide coating has a nearly three-dimensional porous structure, and copper is incorporated into the coating without changing the structure of the coating. In vitro experiments found that the coating can promote the adhesion, proliferation and differentiation of MC3T3-E1 cells. In vivo experiments further confirmed that the titanium copper-titanium dioxide microporous coating can promote the osseointegration of titanium implants. In conclusion, copper-titanium dioxide microporous coatings can be prepared by microarc oxidation, which can improve the biological activity and biocompatibility of titanium, promote new bone formation and demonstrate good osteoinductive properties. Therefore, the use of this coating in orthopedics has potential clinical application.

scholarly journals Manufacturing of bilayer bioceramic coatings on surface of titanium alloy Ti-6Al-4V

2021 ◽  
pp. 69-74
Keyword(s):  
Titanium Alloy ◽  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Stoichiometric Ratio ◽  
Detonation Spraying ◽  
Integrated Technology ◽  
Bioceramic Coatings ◽  
Bilayer Coatings

This paper discusses some properties of bioceramic bilayer coatings on Ti-6Al-4V substrates obtained by an integrated technology including microarc oxidation and detonation spraying of calcium phosphate (Ca-P) layers. Ca-P coatings were deposited from feedstock HAp parti-cles. As a result, bilayer systems were obtained: TiO2 (with a thickness of  2–3 μm) and sub-sequent Ca-P (with a thickness of  100–150 μm) coatings. These coatings were characterized by SEM, XRD and EDX. The coatings contained only biocompatible phases – anatase, hy-droxyapatite and tricalcium phosphate. No cytotoxic components have been registered. The stoichiometric ratio was Ca/P  1.56–1.86. The conclusion is made about the prospects of the proposed integrated technology for manufacturing bilayer ceramics to titanium implants.

The Effect of Microarc Oxidation Ceramic Coating on Sensory Reconstruction around Implants Using Cultured Schwann Cells

2007 ◽  
Vol 342-343 ◽  
pp. 697-700
Author(s):  
Quan Yuan ◽  
Zhen Tan ◽  
Ping Gong ◽  
Rong Rong Nie ◽  
Xiao Yu Li ◽  
...  
Keyword(s):  
Dental Implants ◽  
Schwann Cells ◽  
Ceramic Coating ◽  
Pure Titanium ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Porous Coating ◽  
Enzyme Linked Immunosorbent Assay ◽  
Total Protein Content ◽  
Sensory Reconstruction

Osseointegrated titanium implants have been widely used in clinics for replacement of missing teeth. However, sensory perception thresholds of implants were 10 to 100 times higher than those of natural teeth. The purpose of this study was to evaluate the effect of the ceramic coating generated by microarc oxidation (MAO) on sensory reconstruction around dental implants. The MAO coating was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and scanning probe microscope (SPM). Moreover, the chemical composition was analyzed with an energy dispersive spectroscope (EDS). Using smooth titanium discs as a control, Schwann cell responses to the coating were evaluated by SEM, MTT assay, total protein content and leakage of cytosolic lactate dehydrogenase (LDH) activity. In addition, the amount of brain-derived neurotrophic factor (BDNF) secreted by Schwann cells was measured by Enzyme-Linked Immunosorbent Assay (ELISA). It was observed that the porous coating had separated and homogeneous micropores in the diameter of 1-2 μm. The concentrations of Ca and P in the layer were 8.63% and 5.23% respectively. The cell morphology, proliferation and secretion of BDNF were unaffected. The results indicated that the MAO ceramic coating developed on the surface of pure titanium had good biocompatibility with Schwann cells for sensory reconstruction around dental implants.

Biocompatibility of titanium implants modified by microarc oxidation and hydroxyapatite coating

2005 ◽  
Vol 73A (1) ◽  
pp. 48-54 ◽  
Author(s):  
Long-Hao Li ◽  
Hae-Won Kim ◽  
Su-Hee Lee ◽  
Young-Min Kong ◽  
Hyoun-Ee Kim
Keyword(s):  
Microarc Oxidation ◽  
Titanium Implants ◽  
Hydroxyapatite Coating

MC3T3-E1 cell response of amorphous phase/TiO2 nanocrystal composite coating prepared by microarc oxidation on titanium

2014 ◽  
Vol 39 ◽  
pp. 186-195 ◽  
Author(s):  
Rui Zhou ◽  
Daqing Wei ◽  
Haoyue Yang ◽  
Wei Feng ◽  
Su Cheng ◽  
...  
Keyword(s):  
Composite Coating ◽  
Amorphous Phase ◽  
Cell Response ◽  
Microarc Oxidation

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

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