scholarly journals Osteoblast Response to Copper-Doped Microporous Coatings on Titanium for Improved Bone Integration

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Lai-jie Wang ◽  
Xiao-hui Ni ◽  
Fei Zhang ◽  
Zhi Peng ◽  
Fu-xun Yu ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Biological Activity ◽  
Bone Formation ◽  
Titanium Alloys ◽  
Bone Tissue ◽  
Bone Growth ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Material Surface ◽  
Proliferation And Differentiation

AbstractDue to their excellent mechanical properties and good biocompatibility, titanium alloys have become a popular research topic in the field of medical metal implants. However, the surface of the titanium alloy does not exhibit biological activity, which may cause poor integration between the interface of the titanium implant and the interface of the bone tissue and subsequently may cause the implant to fall off. Therefore, surface biological inertness is one of the problems that titanium alloys must overcome to become an ideal orthopedic implant material. Surface modification can improve the biological properties of titanium, thereby enhancing its osseointegration effect. Copper is an essential trace element for the human body, can promote bone formation and plays an important role in maintaining the physiological structure and function of bone and bone growth and development. In this study, a microporous copper-titanium dioxide coating was prepared on the surface of titanium by microarc oxidation. Based on the evaluation of its surface characteristics, the adhesion, proliferation and differentiation of MC3T3-E1 cells were observed. A titanium rod was implanted into the rabbit femoral condyle, and the integration of the coating and bone tissue was evaluated. Our research results show that the microporous copper-titanium dioxide coating has a nearly three-dimensional porous structure, and copper is incorporated into the coating without changing the structure of the coating. In vitro experiments found that the coating can promote the adhesion, proliferation and differentiation of MC3T3-E1 cells. In vivo experiments further confirmed that the titanium copper-titanium dioxide microporous coating can promote the osseointegration of titanium implants. In conclusion, copper-titanium dioxide microporous coatings can be prepared by microarc oxidation, which can improve the biological activity and biocompatibility of titanium, promote new bone formation and demonstrate good osteoinductive properties. Therefore, the use of this coating in orthopedics has potential clinical application.

scholarly journals Bone formation enhanced by induction: Bone growth in titanium implants in rats

1985 ◽  
Vol 56 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Helge Rønningen ◽  
Ludvig F. Solheim ◽  
Norvald Langeland
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Titanium Implants

Bone Growth in Porous Titanium Implants Made by Rapid Prototyping

2006 ◽  
Vol 309-311 ◽  
pp. 1099-1104 ◽  
Author(s):  
Marco A. Lopez-Heredia ◽  
Eric Goyenvalle ◽  
Eric Aguado ◽  
C. Leroux ◽  
M. Dorget ◽  
...  
Keyword(s):  
Compressive Strength ◽  
New Zealand ◽  
Bone Formation ◽  
Rapid Prototyping ◽  
Porous Structure ◽  
Bone Growth ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Interconnected Pore ◽  
New Zealand Rabbits

Two porous titanium implants with interconnected pore size of 800 and 1200 m in diameter, were fabricated by a rapid prototyping method. Their dimensions and structure accomplished the expected design with accuracy and reproducibility. The porosity of titanium was around 60%. The compressive strength and Young’s modulus were comparable to those of cortical bone with values around 80 MPa and 2.7 GPa, respectively. The implants were implanted bilaterally in the femoral epiphysis of 12 New Zealand Rabbits. After 3 and 8 weeks, abundant bone formation was found in the titanium porous structure. This work demonstrates that macroporous titanium with controlled shape and porosity is a good candidate for orthopaedic and maxilofacial applications.

Preparation and Biological Activity of Strontium Modified Titanium Oxide Surface

2021 ◽  
Vol 15 (1) ◽  
pp. 69-74
Author(s):  
Hui-Xing Li ◽  
Quan-Ming Zhao ◽  
Da-Chang Liu ◽  
Xiao-Hui Ni ◽  
Xing-Yuan Zhu ◽  
...  
Keyword(s):  
Biological Activity ◽  
Microarc Oxidation ◽  
Oxide Surface ◽  
X Ray Diffraction ◽  
X Ray ◽  
Tissue Replacement ◽  
Proliferation And Differentiation ◽  
Calcium Glycerophosphate ◽  
Titanium Oxide Surface

Titanium (Ti) is a bone tissue replacement material with excellent mechanical properties. However, Ti has poor biological activity and lacks osseointegrative properties and may trigger inflammation and cause implantation failure. To address these issues, Ti surface modification has become a widely used method. In this study, using microarc oxidation (MAO) technology, a strontium-doped MAO coating was prepared on Ti surfaces in an electrolyte solution comprising calcium acetate, calcium glycerophosphate and strontium acetate. Scanning electron microscopy (SEM) and energy spectrum analysis (EDS) were used to analyze the tissue structure and elemental composition of the coating. The surface phase composition of the coating was analyzed by X-ray diffraction (XRD). We observed the proliferation of cells on the coating surface through in vitro cell experiments. The results showed the surface of the strontium-doped MAO coating is porous, and strontium is evenly distributed on the surface. The coating can promote the adhesion, proliferation and differentiation of osteoblasts and has good biological activity and thus, could potentially be applied to the surface of implants in clinical application.

scholarly journals Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

2016 ◽  
Vol 17 (3) ◽  
pp. 292 ◽  
Author(s):  
Xijiang Zhao ◽  
Tao Wang ◽  
Shi Qian ◽  
Xuanyong Liu ◽  
Junying Sun ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Bone Formation ◽  
Titanium Implants ◽  
Titanium Dioxide Nanotubes ◽  
Doped Titanium Dioxide ◽  
Silicon Doped

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Bone tissue engineering in the greater omentum with computer-aided design/computer-aided manufacturing scaffolds is enhanced by a periosteum transplant

2020 ◽  
Author(s):  
Hendrik Naujokat ◽  
Klaas Loger ◽  
Juliane Schulz ◽  
Yahya Açil ◽  
Jörg Wiltfang
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Computer Aided Design ◽  
Bone Tissue Regeneration ◽  
Histological Evaluation ◽  
Greater Omentum ◽  
Collagen Membrane ◽  
Computer Aided ◽  
3D Printed ◽  
The Impact

Aim: This study aimed to evaluate two different vascularized bone flap scaffolds and the impact of two barrier membranes for the reconstruction of critical-size bone defects. Materials & methods: 3D-printed scaffolds of biodegradable calcium phosphate and bioinert titanium were loaded with rhBMP-2 bone marrow aspirate, wrapped by a collagen membrane or a periosteum transplant and implanted into the greater omentum of miniature pigs. Results: Histological evaluation demonstrated significant bone formation within the first 8 weeks in both scaffolds. The periosteum transplant led to enhanced bone formation and a homogenous distribution in the scaffolds. The omentum tissue grew out a robust vascular supply. Conclusion: Endocultivation using 3D-printed scaffolds in the greater omentum is a very promising approach in defect-specific bone tissue regeneration.

scholarly journals Additive-manufactured Ti-6Al-4 V/Polyetheretherketone composite porous cage for Interbody fusion: bone growth and biocompatibility evaluation in a porcine model

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Pei-I Tsai ◽  
Meng-Huang Wu ◽  
Yen-Yao Li ◽  
Tzu-Hung Lin ◽  
Jane S. C. Tsai ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Lumbar Fusion ◽  
High Porosity ◽  
Ti Alloy ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Intervertebral Fusion ◽  
Porous Ti ◽  
Peek Composite

Abstract Background We developed a porous Ti alloy/PEEK composite interbody cage by utilizing the advantages of polyetheretherketone (PEEK) and titanium alloy (Ti alloy) in combination with additive manufacturing technology. Methods Porous Ti alloy/PEEK composite cages were manufactured using various controlled porosities. Anterior intervertebral lumbar fusion and posterior augmentation were performed at three vertebral levels on 20 female pigs. Each level was randomly implanted with one of the five cages that were tested: a commercialized pure PEEK cage, a Ti alloy/PEEK composite cage with nonporous Ti alloy endplates, and three composite cages with porosities of 40, 60, and 80%, respectively. Micro-computed tomography (CT), backscattered-electron SEM (BSE-SEM), and histological analyses were performed. Results Micro-CT and histological analyses revealed improved bone growth in high-porosity groups. Micro-CT and BSE-SEM demonstrated that structures with high porosities, especially 60 and 80%, facilitated more bone formation inside the implant but not outside the implant. Histological analysis also showed that bone formation was higher in Ti alloy groups than in the PEEK group. Conclusion The composite cage presents the biological advantages of Ti alloy porous endplates and the mechanical and radiographic advantages of the PEEK central core, which makes it suitable for use as a single implant for intervertebral fusion.

scholarly journals In Vivo Study for Clinical Application of Dental Stem Cell Therapy Incorporated with Dental Titanium Implants

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 381
Author(s):  
Hyunmin Choi ◽  
Kyu-Hyung Park ◽  
Narae Jung ◽  
June-Sung Shim ◽  
Hong-Seok Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Alveolar Bone ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Osteogenic Potential ◽  
Induction Medium ◽  
Radiographic Analysis ◽  
New Bone Formation

The aim of this study was to investigate the behavior of dental-derived human mesenchymal stem cells (d-hMSCs) in response to differently surface-treated implants and to evaluate the effect of d-hMSCs on local osteogenesis around an implant in vivo. d-hMSCs derived from alveolar bone were established and cultured on machined, sandblasted and acid-etched (SLA)-treated titanium discs with and without osteogenic induction medium. Their morphological and osteogenic potential was assessed by scanning electron microscopy (SEM) and real-time polymerase chain reaction (RT-PCR) via mixing of 5 × 106 of d-hMSCs with 1 mL of Metrigel and 20 μL of gel-cell mixture, which was dispensed into the defect followed by the placement of customized mini-implants (machined, SLA-treated implants) in New Zealand white rabbits. Following healing periods of 2 weeks and 12 weeks, the obtained samples in each group were analyzed radiographically, histomorphometrically and immunohistochemically. The quantitative change in osteogenic differentiation of d-hMSCs was identified according to the type of surface treatment. Radiographic analysis revealed that an increase in new bone formation was statistically significant in the d-hMSCs group. Histomorphometric analysis was in accordance with radiographic analysis, showing the significantly increased new bone formation in the d-hMSCs group regardless of time of sacrifice. Human nuclei A was identified near the area where d-hMSCs were implanted but the level of expression was found to be decreased as time passed. Within the limitations of the present study, in this animal model, the transplantation of d-hMSCs enhanced the new bone formation around an implant and the survival and function of the stem cells was experimentally proven up to 12 weeks post-sacrifice.

Ions release behavior of vanadium-doped mesoporous bioactive glass particles and effect on BMSCs osteogenic differentiation via FAK/MAPK signaling pathway

2021 ◽  
Author(s):  
Jiangfeng Li ◽  
Junying Li ◽  
Yuhao Wei ◽  
Na Xu ◽  
Jingtao Li ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bioactive Glass ◽  
Osteogenic Differentiation ◽  
Bone Growth ◽  
Mapk Signaling ◽  
Release Behavior ◽  
Pentavalent Vanadium ◽  
Important Trace Element ◽  
Vanadium Ions ◽  
Mesoporous Bioactive Glass

Vanadium is an important trace element in bone to involve in bone metabolism, bone formation, and bone growth, but roles of various vanadium ions, especially pentavalent vanadium, in bone tissue...

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

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