RIP140 Gene and Protein Expression Levels are Downregulated in Visceral Adipose Tissue in Human Morbid Obesity

2009 ◽  
Vol 19 (6) ◽  
pp. 771-776 ◽  
Author(s):  
Victoria Catalán ◽  
Javier Gómez-Ambrosi ◽  
Amaia Lizanzu ◽  
Amaia Rodríguez ◽  
Camilo Silva ◽  
...  
Keyword(s):  
Morbid Obesity ◽  
Adipose Tissue ◽  
Protein Expression ◽  
Visceral Adipose Tissue ◽  
Expression Levels ◽  
Gene And Protein Expression ◽  
Visceral Adipose

Influence of Morbid Obesity and Insulin Resistance on Gene Expression Levels of AQP7 in Visceral Adipose Tissue and AQP9 in Liver

2008 ◽  
Vol 18 (6) ◽  
pp. 695-701 ◽  
Author(s):  
Victoria Catalán ◽  
Javier Gómez-Ambrosi ◽  
Carlos Pastor ◽  
Fernando Rotellar ◽  
Camilo Silva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Morbid Obesity ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Expression Levels ◽  
Visceral Adipose ◽  
Gene Expression Levels

scholarly journals Adipose Tissues Characteristics of Normal, Obesity, and Type 2 Diabetes in Uygurs Population

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jun Zhang ◽  
Zhiwei Zhang ◽  
Yulei Ding ◽  
Peng Xu ◽  
Tingting Wang ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Adipose Tissue ◽  
Blood Glucose ◽  
Visceral Adipose Tissue ◽  
Tissue Expression ◽  
Normal Group ◽  
Expression Levels ◽  
Visceral Adipose ◽  
Male Subjects ◽  
Female Subjects

Our results showed that, at the same BMI level, Uygurs have greater WHR values, abdominal visceral fat content, and diabetes risks than Kazaks. In addition, values of HDL-C in Uygur subjects were lower than those in Kazak subjects, and values of creatinine, uric acid, diastolic blood pressure, blood glucose, and fructosamine in Uygur male subjects were lower than those in Kazak male subjects. In contrast, systolic blood pressure values in Uygur subjects were greater than those in Kazak subjects, and blood glucose values were greater in Uygur female subjects than in Kazak female subjects. Additionally, in Uygurs, visceral adipose tissue expression levels ofTBX1andTCF21were greater in obesity group than in normal and T2DM groups and lower in T2DM group than in normal group (P<0.01). The visceral adipose tissue expression levels ofAPNin normal group was greater than those in obesity and T2DM groups, and visceral adipose tissue expression levels ofTNF-αandMCP-1in normal group were lower than those in obesity and T2DM groups (P<0.01). In conclusion, T2DM in Uygurs was mainly associated with not only distribution of adipose tissue in body, but also change in metabolic activity and adipocytokines secretion of adipose tissue.

Adipocyte triglyceride lipase expression in human obesity

2007 ◽  
Vol 293 (4) ◽  
pp. E958-E964 ◽  
Author(s):  
Gregory R. Steinberg ◽  
Bruce E. Kemp ◽  
Matthew J. Watt
Keyword(s):  
Adipose Tissue ◽  
Protein Expression ◽  
Mrna Expression ◽  
Visceral Adipose Tissue ◽  
Lipase Activity ◽  
Subcutaneous Adipose Tissue ◽  
Lipolytic Enzymes ◽  
Obese Subjects ◽  
Visceral Adipose ◽  
Subcutaneous Adipose

We have investigated the gene and protein expression of adipose triglyceride lipase (ATGL) and triglyceride (TG) lipase activity from subcutaneous and visceral adipose tissue of lean and obese subjects. Visceral and subcutaneous adipose tissue was obtained from 16 age-matched lean and obese subjects during abdominal surgery. Tissues were analyzed for mRNA expression of lipolytic enzymes by real-time quantitative PCR. ATGL protein content was assessed by Western blot and TG lipase activity by radiometric assessment. Subcutaneous and visceral adipose tissue of obese subjects had elevated mRNA expression of PNPLA2 (ATGL) and other lipases including PNPLA3, PNPLA4, CES1, and LYPLAL1 ( P < 0.05). Surprisingly, ATGL protein expression and TG lipase activity were reduced in subcutaneous adipose tissue of obese subjects. Immunoprecipitation of ATGL reduced total TG lipase activity in adipose lysates by 70% in obese and 83% in lean subjects. No significant differences in the ATGL activator CGI-58 mRNA levels ( ABHD5) were associated with obesity. These data demonstrate that ATGL is important for efficient TG lipase activity in humans. They also demonstrate reduced ATGL protein expression and TG lipase activity despite increased mRNA expression of ATGL and other novel lipolytic enzymes in obesity. The lack of correlation between ATGL protein content and in vitro TG lipase activity indicates that small decrements in ATGL protein expression are not responsible for the reduction in TG lipase activity observed here in obesity, and that posttranslational modifications may be important.

Differences in AMPK expression between subcutaneous and visceral adipose tissue in morbid obesity

2010 ◽  
Vol 163 (1-3) ◽  
pp. 31-36 ◽  
Author(s):  
O. Martínez-Agustin ◽  
J.J. Hernández-Morante ◽  
E. Martínez-Plata ◽  
F. Sánchez de Medina ◽  
M. Garaulet
Keyword(s):  
Morbid Obesity ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Visceral Adipose

scholarly journals PO-280 Effects of different exercise on autophagy and inflammation in visceral adipose tissue of obese

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Yongjun Zhao ◽  
Jing Wang ◽  
Yuxiu He
Keyword(s):  
Adipose Tissue ◽  
Protein Expression ◽  
Visceral Adipose Tissue ◽  
Exercise Intervention ◽  
Intervention Group ◽  
Diet Group ◽  
Normal Diet ◽  
Fat Reduction ◽  
Visceral Adipose ◽  
The Relationship

Objective To compare the effectiveness of endurance exercise and resistance exercise in fat reduction, then to examine the impact of exercise on autophagy activity and inflammation response in visceral adipose tissue in obesity, finally to explore the relationship between autophagy and inflammation in adipose tissue after exercise. Methods 42 obese mice were randomly divided into four groups as follows: high fat diet group (HC, n=9), normal diet group (NC, n=9), normal diet combined with endurance exercise intervention group (NE, n=12), and normal diet combined with resistance exercise intervention group (NR, n=12). NE and NR group conducted treadmill and ladder climbing exercise respectively for 8 weeks. Then to detect the gene and protein expression of autophagy, inflammation, ER stress and antioxidant markers using RT-PCR and WB, in addition, TEM and IHC were used to observe the autophagosome in visceral adipose tissue. Results BW, Lee’s index and BFI were significantly decreased in all three intervention groups, and there is a great decreasing in the two exercise group, but no difference between them. Atg5, Becn1 expression and LC3II/I were decreased significantly in NE and NR group compared with HC, meanwhile p62 expression were significantly increased. When compared with NC group, p62 expression were significantly increased in NE and not happened in NR group. Becn1 mRNA expression increased and p62 protein expression decreased significantly in NR group when compared with NE group. IL-1β was decreased significantly in NC, NE and NR group compared with HC. In addition, IL-6 and IL-10 protein expression increased significantly both in NE and NR group. When compared with NC group, IL-6 and IL-10 protein expression increased and IL-1β was decreased significantly both in NE and NR group except IL-6 protein expression in NR group. IL-6 increased and IL-10 decreased significantly in NR group when compared with NE group. Finally we found that IL-10 showed a negative correlation with almost every autophagy markers used in this study. Conclusions The effectiveness of 8wks different exercise intervention had no difference in fat reduction. The autophagic activity of visceral adipose tissue was inhibited after exercise, especially after aerobic exercise. Autophagy and inflammation enjoy the same trend before and after exercise intervention in visceral adipose tissue in obesity, and the IL-10 is the most sensitive factor in reflecting the relationship between autophagy and inflammation.

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