Salinity tolerance of Aegilops cylindrica genotypes collected from hyper-saline shores of Uremia Salt Lake using physiological traits and SSR markers

2014 ◽  
Vol 36 (8) ◽  
pp. 2243-2251 ◽  
Author(s):  
Mahbube Arabbeigi ◽  
Ahmad Arzani ◽  
Mohamad Mahdi Majidi ◽  
Razie Kiani ◽  
Badraldin Ebrahim Sayed Tabatabaei ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Salt Lake ◽  
Physiological Traits ◽  
Aegilops Cylindrica

Association of SSR markers and morpho-physiological traits associated with salinity tolerance in sugar beet (Beta vulgaris L.)

Euphytica ◽  
2015 ◽  
Vol 205 (3) ◽  
pp. 785-797 ◽  
Author(s):  
Zahra Abbasi ◽  
Mohammad Mahdi Majidi ◽  
Ahmad Arzani ◽  
Abazar Rajabi ◽  
Parisa Mashayekhi ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Physiological Traits

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

scholarly journals Evaluation of Rice Germplasm under Salt Stress at the Seedling Stage through SSR Markers

2013 ◽  
Vol 3 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M Al-Amin ◽  
MM Islam ◽  
SN Begum ◽  
MS Alam ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Dry Matter ◽  
Growth Parameters ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Salt Susceptible

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

The Development of a Diatom Database for Inferring Lake Salinity, Western Victoria, Australia: Towards a Quantitative Approach for Reconstructing Past Climates

1997 ◽  
Vol 45 (3) ◽  
pp. 389 ◽  
Author(s):  
Peter A. Gell
Keyword(s):  
Salinity Tolerance ◽  
Salt Lake ◽  
Data Sets ◽  
Diatom Assemblages ◽  
Tolerance Limits ◽  
Data Set ◽  
Lower Salinity ◽  
Western Victoria ◽  
Lake Waters ◽  
Lake Salinity

The development of a modern data set of 156 diatom samples from salt lakes has provided evidence of the tolerance of a large number of taxa to the salinity of lake waters. Thirty taxa have been recorded from 30 or more samples and so have been well characterised. A further 42 taxa have been recorded from 10 or more samples. The lakes sampled range in salinity from the freshwater–oligosaline boundary to well into the hypersaline range, so the upper and lower salinity tolerance limits of many species were investigated. Canonical correspondence analysis of the data set showed that salinity was the most important of the tested parameters influencing the diatom assemblages in the samples. Randomisation tests have provided correlation values between measured and predicted salinity comparable with those gained from other major salt lake diatom data sets, suggesting that this set is a good predictor of lake salinity.

Morpho-Physiological Traits Imparting Salinity Tolerance in Maize (Zea Mays L.) Hybrids under Saline Water Irrigation in Vertisols

2016 ◽  
Author(s):  
Indivar Prasad ◽  
G. Gururaja Rao ◽  
Anil R. Chinchmalatpure ◽  
Shrvan Kumar ◽  
Nikam Vinayak Ramesh ◽  
...  
Keyword(s):  
Zea Mays ◽  
Salinity Tolerance ◽  
Zea Mays L ◽  
Saline Water ◽  
Physiological Traits ◽  
Saline Water Irrigation

scholarly journals Genome-wide selection scans integrated with association mapping reveal mechanisms of physiological adaptation across a salinity gradient in killifish

2018 ◽  
Author(s):  
Reid S. Brennan ◽  
Timothy M. Healy ◽  
Heather J. Bryant ◽  
Man Van La ◽  
Patricia M. Schulte ◽  
...  
Keyword(s):  
Natural Selection ◽  
Salinity Tolerance ◽  
Hypoxia Tolerance ◽  
Physiological Traits ◽  
Adaptive Divergence ◽  
Genome Wide ◽  
Native Populations ◽  
Genomic Regions ◽  
Insight Into ◽  
Freshwater Environments

AbstractAdaptive divergence between marine and freshwater environments is important in generating phyletic diversity within fishes, but the genetic basis of adaptation to freshwater habitats remains poorly understood. Available approaches to detect adaptive loci include genome scans for selection, but these can be difficult to interpret because of incomplete knowledge of the connection between genotype and phenotype. In contrast, genome wide association studies (GWAS) are powerful tools for linking genotype to phenotype, but offer limited insight into the evolutionary forces shaping variation. Here, we combine GWAS and selection scans to identify loci important in the adaptation of complex physiological traits to freshwater environments. We focused on freshwater (FW)-native and brackish water (BW)-native populations of the Atlantic killifish (Fundulus heteroclitus) as well as a population that is a natural admixture of these two populations. We measured phenotypes for multiple physiological traits that differ between populations and that may contribute to adaptation across osmotic niches (salinity tolerance, hypoxia tolerance, metabolic rate, and body shape) and used a reduced representation approach for genome-wide genotyping. Our results show patterns of population divergence in physiological capabilities that are consistent with local adaptation. Selection scans between BW-native and FW-native populations identified genomic regions that presumably aect fitness between BW and FW environments, while GWAS revealed loci that contribute to variation for each physiological trait. There was substantial overlap in the genomic regions putatively under selection and loci associated with the measured physiological traits, suggesting that these phenotypes are important for adaptive divergence between BW and FW environments. Our analysis also implicates candidate genes likely involved in physiological capabilities, some of which validate a priori hypotheses. Together, these data provide insight into the mechanisms that enable diversification of fishes across osmotic boundaries.Author SummaryIdentifying the genes that underlie adaptation is important for understanding the evolutionary process, but this is technically challenging. We bring multiple lines of evidence to bear for identifying genes that underlie adaptive divergence. Specifically, we integrate genotype-phenotype association mapping with genome-wide scans for signatures of natural selection to reveal genes that underlie phenotypic variation and that are adaptive in populations of killifish that are diverging between marine and freshwater environments. Because adaptation is likely manifest in multiple physiological traits, we focus on hypoxia tolerance, salinity tolerance, and metabolic rate; traits that are divergent between marine and freshwater populations. We show that each of these phenotypes is evolving by natural selection between environments; genetic variants that contribute to variation in these physiological traits tend to be evolving by natural selection between marine and freshwater populations. Furthermore, one of our top candidate genes provides a mechanistic explanation for previous hypotheses that suggest the adaptive importance of cellular tight junctions. Together, these data demonstrate a powerful approach to identify genes involved in adaptation and help to reveal the mechanisms enabling transitions of fishes across osmotic boundaries.

scholarly journals Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jing Zhang ◽  
Hui Li ◽  
Yiwei Jiang ◽  
Huibin Li ◽  
Zhipeng Zhang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Heat Tolerance ◽  
Perennial Ryegrass ◽  
Physiological Traits ◽  
Grass Species ◽  
Cool Season ◽  
Catabolic Genes ◽  
Heat Tolerant ◽  
Cool Season Grass

Abstract Background Identification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenne L.). Results Plants of 98 accessions were subjected to heat stress (35/30 °C, day/night) or optimal growth temperature (25/20 °C) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (− 0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), including LpNYC1, LpNOL, LpSGR, and LpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2 > 0.05, p < 0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL. Conclusion The result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.

Sign in / Sign up

Export Citation Format

Share Document