Sorting nexins, a component of retromer complex, interact with SRK in Brassica oleracea

The FERM-like domain–containing sorting nexins of the SNX17/SNX27/SNX31 family have been proposed to mediate retrieval of transmembrane proteins from the lysosomal pathway. In this paper, we describe a stable isotope labeling with amino acids in culture–based quantitative proteomic approach that allows an unbiased, global identification of transmembrane cargoes that are rescued from lysosomal degradation by SNX17. This screen revealed that several integrins required SNX17 for their stability, as depletion of SNX17 led to a loss of β1 and β5 integrins and associated a subunits from HeLa cells as a result of increased lysosomal degradation. SNX17 bound to the membrane distal NPXY motif in β integrin cytoplasmic tails, thereby preventing lysosomal degradation of β integrins and their associated a subunits. Furthermore, SNX17-dependent retrieval of integrins did not depend on the retromer complex. Consistent with an effect on integrin recycling, depletion of SNX17 also caused alterations in cell migration. Our data provide mechanistic insight into the retrieval of internalized integrins from the lysosomal degradation pathway, a prerequisite for subsequent recycling of these matrix receptors.

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The Retromer Complex and Sorting Nexins in Neurodegenerative Diseases

Frontiers in Aging Neuroscience ◽

10.3389/fnagi.2018.00079 ◽

2018 ◽

Vol 10 ◽

Cited By ~ 19

Author(s):

Hongfeng Zhang ◽

Timothy Huang ◽

Yujuan Hong ◽

Weijie Yang ◽

Xian Zhang ◽

...

Keyword(s):

Neurodegenerative Diseases ◽

Sorting Nexins ◽

Retromer Complex

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Structural characterisation of retromer complexes

Acta Crystallographica Section A Foundations and Advances ◽

10.1107/s2053273314095825 ◽

2014 ◽

Vol 70 (a1) ◽

pp. C417-C417

Author(s):

Suzanne Norwood ◽

Natalya Leneva ◽

Rajesh Ghai ◽

Nathan Cowieson ◽

Anthony Duff ◽

...

Keyword(s):

Protein Complex ◽

Structural Information ◽

Critical Role ◽

Retrograde Transport ◽

Scattering Data ◽

Sorting Nexins ◽

Structural Characterisation ◽

Retromer Complex ◽

Biological Studies ◽

Synergistic Approach

Retromer is a peripheral membrane protein complex that plays a critical role in a broad range of physiological, developmental and pathological processes including Wnt signalling, toxin transport and amyloid production in Alzheimer's disease. The classical mammalian retromer complex consists of a core heterotrimeric cargo recognition sub-complex (VPS26, VPS29 and VPS35) associated with a dimer of proteins from the SNX–BAR sorting nexin family that drives membrane deformation and tubulation. By recruiting the cargo-selective sub-complex to the forming tubules, the SNX–BAR coat complex mediates the retrograde transport of proteins from endosomes to the trans-Golgi network. Recent studies, however, have highlighted the molecular and functional diversity of retromer and the identification of new interacting proteins has revealed that the role of retromer extends to aspects of endosome-to-plasma membrane sorting and regulation of signalling events. Emerging evidence indicates that cargo specificity is mediated by specific sorting nexins. These include SNX3, involved in the trafficking of the Wntless/MIG-14 protein, and SNX27, a PX-FERM protein that mediates the retrieval of the β2-adrenergic receptor.Using the MX and SAXS/WAXS beamlines at the Australian Synchrotron, we have acquired crystallographic and small angle scattering data to determine how the core cargo recognition sub-complex assembles and to characterise the retromer-associated sorting nexins. We are using this structural information in combination with biochemical and biological studies in a synergistic approach to understand retromer-mediated endosomal protein sorting and how this fascinating protein complex contributes to a diverse set of cellular processes. The retromer complex is conserved across all eukaryotes. We are also currently exploring the structure of these proteins in the thermophilic fungus Chaetomium thermophilum and initial crystallisation experiments have produced some promising results.

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Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0409558102 ◽

2005 ◽

Vol 102 (42) ◽

pp. 15173-15177 ◽

Cited By ~ 50

Author(s):

C. T. Griffin ◽

J. Trejo ◽

T. Magnuson

Keyword(s):

Genetic Evidence ◽

Sorting Nexins ◽

Retromer Complex

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Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling

The Journal of Cell Biology ◽

10.1083/jcb.200609161 ◽

2007 ◽

Vol 177 (1) ◽

pp. 115-125 ◽

Cited By ~ 108

Author(s):

Todd I. Strochlic ◽

Thanuja Gangi Setty ◽

Anand Sitaram ◽

Christopher G. Burd

Keyword(s):

Plasma Membrane ◽

Golgi Apparatus ◽

Rab Gtpase ◽

Endocytic Recycling ◽

Sorting Nexins ◽

Sorting Nexin ◽

Retromer Complex ◽

Iron Transporter ◽

Endocytic Sorting ◽

Recycling Pathway

Amajor function of the endocytic system is the sorting of cargo to various organelles. Endocytic sorting of the yeast reductive iron transporter, which is composed of the Fet3 and Ftr1 proteins, is regulated by available iron. When iron is provided to iron-starved cells, Fet3p–Ftr1p is targeted to the lysosome-like vacuole and degraded. In contrast, when iron is not available, Fet3p–Ftr1p is maintained on the plasma membrane via an endocytic recycling pathway requiring the sorting nexin Grd19/Snx3p, the pentameric retromer complex, and the Ypt6p Golgi Rab GTPase module. A recycling signal in Ftr1p was identified and found to bind directly to Grd19/Snx3p. Retromer and Grd19/Snx3p partially colocalize to tubular endosomes, where they are physically associated. After export from the endosome, Fet3p–Ftr1p transits through the Golgi apparatus for resecretion. Thus, Grd19/Snx3p, functions as a cargo-specific adapter for the retromer complex, establishing a precedent for a mechanism by which sorting nexins expand the repertoire of retromer-dependent cargos.

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Human Papillomavirus type 16 L2 recruits both retromer and retriever complexes during retrograde trafficking of the viral genome to the cell nucleus.

Journal of Virology ◽

10.1128/jvi.02068-20 ◽

2020 ◽

Cited By ~ 1

Author(s):

David Pim ◽

Justyna Broniarczyk ◽

Abida Siddiqa ◽

Paola Massimi ◽

Lawrence Banks

Keyword(s):

Human Papillomavirus ◽

Viral Genome ◽

Direct Interaction ◽

Hpv Infection ◽

Infection Process ◽

Sorting Nexins ◽

Minor Capsid Protein ◽

Human Papillomavirus Type 16 ◽

Retromer Complex ◽

Shed Light

Previous studies have identified an interaction between Human Papillomavirus L2 minor capsid protein and sorting nexins 17 and 27 (SNX17 and SNX27) during virus infection. Further studies show involvement of both retromer and retriever complexes in this process, since knockdown of proteins from either complex impairs infection. In this study, we show that HPV L2 and EdU-labelled pseudovirions colocalize with both retromer and retriever, with components of each complex being bound by L2 during infection. We also show that both sorting nexins may interact with either of the recycling complexes, but that the interaction between SNX17 and HPV16 L2 is not responsible for retriever recruitment during infection, instead being required for retromer recruitment. Further, we show that retriever recruitment most likely involves direct interaction between L2 and the C16orf62 subunit of retriever, in a similar manner to its interaction with the VPS35 subunit of retromer. IMPORTANCE Previous studies identified sorting nexins 17 and 27, as well as the retromer complex, as playing a role in HPV infection. This study shows that the newly-identified retriever complex also plays an important role and begins to shed light on how both sorting nexins contribute to retromer and retriever recruitment during the infection process.

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Tronchuda cabbage (Brassica oleracea L. var. costata DC) leaf free amino acid profile

Planta Medica ◽

10.1055/s-0028-1084544 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

BM Silva ◽

AP Oliveira ◽

DM Pereira ◽

C Sousa ◽

RM Seabra ◽

...

Keyword(s):

Amino Acid ◽

Brassica Oleracea ◽

Free Amino Acid ◽

Free Amino ◽

Amino Acid Profile ◽

Tronchuda Cabbage ◽

Brassica Oleracea L

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EFFECT OF DIFFERENT DOSES OF BORON ON GROWTH AND YIELD OF OFF-SEASON CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS) IN ARGHAKHANCHI, NEPAL

Reviews In Food And Agriculture ◽

10.26480/rfna.01.2020.12.17 ◽

2020 ◽

Vol 1 (1) ◽

pp. 12-17

Author(s):

Srijana Pandey ◽

Sapana Parajuli ◽

Biplov Oli ◽

Surya Dhungana

Keyword(s):

Brassica Oleracea ◽

Growth Parameters ◽

Block Design ◽

Leaf Length ◽

Growth And Yield ◽

Cost Ratio ◽

Benefit Cost Ratio ◽

Net Returns ◽

Benefit Cost ◽

Different Doses

The research was conducted at Beltari Sandhikharka-10, Arghakhanchi district to study about the effect of various doses of boron on growth and yield attributing characters of cauliflower (Brassica oleracea var. botrytis) during off-season from February to June in 2020. Five treatments; B0 (control/no application), B1: 2kg/ha, B2: 4kg/ha, B3: 6kg/ha, B4: 8kg/ha which are the different doses of boron application were laid out in Randomized Complete Block Design (RCBD) with 4 replications. The growth parameters like plant height, number of leaves per plant leaf length and leaf breadth of largest leaf were recorded multiple times with 15 days interval. Days to curd initiation and Days from curd initiation to harvest were recorded by regular field observation. The yield parameters; curd weight with leaves, Marketable curd weight, average curd yield, incidence of hollowing were recorded during harvest. The economics from application of boron doses in cauliflower production was obtained in terms of cost of cultivation, gross returns, net returns, and benefit cost ratio. The result showed that the growth and yield attributing characters of cauliflower were significantly affected by application of boron in which the value of these parameters was found significantly higher in B1 followed by other treatments. The average curd yield of cauliflower in B1 was found to be 12.39 mt/ha which was 48.92% higher than that of B0 with curd yield 8.32 mt/ha. Similarly, the net return was found significantly the highest in B1 and the lowest in B0. The result showed that application of boron in the range of 2-4 kg/ha is preferred to control and higher doses in respect of both productivity and economics.

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