Effects of carp and tuna oils on 5-fluorouracil-induced antitumor activity and side effects in sarcoma 180-bearing mice

Himatanthus drasticus, also known as janaguba, is used popularly in Brazil's Northeastern region in the treatment of cancer. However, no scientific reports are available. The present study is the first investigation on the antitumor activity of crude methanolic extract from Himatanthus drasticus leaves against Sarcoma 180 tumor and on its side effects including acute oral toxicity. The OECD 423 methodology was used to study acute oral toxicity, and the STOCK methodology to assess antitumor activity. The crude extract showed low toxicity at the tested doses (50, 300 and 2000 mg/kg) administered orally. The histopathological analyses demonstrated alterations in liver lung, spleen and kidney. It also showed activity against Sarcoma 180 tumor in male Swiss albino mice, evidencing tumor growth inhibition of 67.7% and 68% at 300 mg/kg and 400 mg/kg doses, respectively.

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Tissue Distribution and Systemic Toxicity Evaluation of Raloxifene Targeted Polymeric Micelles of Poly (Styrene-Maleic Acid)-Poly (Amide- Ether-Ester-Imide)-Poly (Ethylene Glycol) Loaded With Docetaxel in Breast Cancer Bearing Mice

Recent Patents on Anti-Cancer Drug Discovery ◽

10.2174/1574892814666190919163731 ◽

2019 ◽

Vol 14 (3) ◽

pp. 280-291 ◽

Cited By ~ 1

Author(s):

Jaleh Varshosaz ◽

Farshid Hassanzadeh ◽

Batool Hashemi-Beni ◽

Mohsen Minaiyan ◽

Saeedeh Enteshari

Keyword(s):

Side Effects ◽

Antitumor Activity ◽

Ethylene Glycol ◽

Tissue Distribution ◽

Tumor Cells ◽

Maleic Acid ◽

Polymeric Micelles ◽

Poly Ethylene Glycol ◽

Poly Ethylene ◽

Ether Ester

Background: Due to the low water solubility of Docetaxel (DTX), it is formulated with ethanol and Tween 80 with lots of side effects. For this reason, special attention has been paid to formulate it in new drug nano-carriers. Objective: The goal of this study was to evaluate the safety, antitumor activity and tissue distribution of the novel synthesized Raloxifene (RA) targeted polymeric micelles. Methods: DTX-loaded RA-targeted polymeric micelles composed of poly(styrene-maleic acid)- poly(amide-ether-ester-imide)-poly(ethylene glycol) (SMA-PAEE-PEG) were prepared and their antitumor activity was studied in MC4-L2 tumor-bearing mice compared with non-targeted micelles and free DTX. Safety of the micelles was studied by Hematoxylin and Eosin (H&E) staining of tumors and major organs of the mice. The drug accumulation in the tumor and major organs was measured by HPLC method. Results: The results showed better tumor growth inhibition and increased survival of mice treated with DTX-loaded in targeted micelles compared to the non-targeted micelles and free DTX. Histopathological studies, H&E staining of tumors and immunohistochemical examination showed the potential of DTX-loaded RA-targeted micelles to inhibit tumor cells proliferation. The higher accumulation of the DTX in the tumor tissue after injection of the micelles compared to the free DTX may indicate the higher uptake of the targeted micelles by the G-Protein-Coupled Estrogen Receptors (GPER). Conclusion: The results indicate that RA-conjugated polymeric micelles may be a strong and effective drug delivery system for DTX therapy and uptake of the drug into tumor cells, and overcome the disadvantages and side effects of conventional DTX.

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Production of Bioactive Compounds with Antitumor Activity Against Sarcoma 180 by Pleurotus sajor-caju

Journal of Medicinal Food ◽

10.1089/jmf.2012.0267 ◽

2013 ◽

Vol 16 (11) ◽

pp. 1004-1012 ◽

Cited By ~ 8

Author(s):

Ivaneliza Simionato Assis ◽

Mariane Bonatti Chaves ◽

Marcia Luciane Lange Silveira ◽

Regina Maria Miranda Gern ◽

Elisabeth Wisbeck ◽

...

Keyword(s):

Antitumor Activity ◽

Bioactive Compounds ◽

Sarcoma 180 ◽

Pleurotus Sajor Caju

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The antitumor activity and toxic side effects of polyethyleneglycol-coated liposome containing adriamycin.

Drug Delivery System ◽

10.2745/dds.10.181 ◽

1995 ◽

Vol 10 (3) ◽

pp. 181-187 ◽

Cited By ~ 1

Author(s):

Yasuyuki Sadzuka ◽

Shin-ichiro Nakai ◽

Atsuo Miyagishima ◽

Yasuo Nozawa ◽

Sadao Hirota

Keyword(s):

Side Effects ◽

Antitumor Activity

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Chemical composition, antitumor activity, and toxicity of essential oil from the leaves of Lippia microphylla

Zeitschrift für Naturforschung C ◽

10.1515/znc-2014-4138 ◽

2015 ◽

Vol 70 (5-6) ◽

pp. 129-137 ◽

Cited By ~ 5

Author(s):

Aline L. Xavier ◽

João Carlos L.R. Pita ◽

Monalisa T. Brito ◽

Déborah R.P. Meireles ◽

Josean F. Tavares ◽

...

Keyword(s):

Chemical Composition ◽

Antitumor Activity ◽

Liver Function ◽

Essential Oil ◽

Pharmacological Study ◽

Hematological Toxicity ◽

Sarcoma 180 ◽

Hemolytic Assay ◽

In Vivo Antitumor Activity

Abstract The chemical composition, antitumor activity and toxicity of the essential oil from Lippia microphylla leaves (OEL) were investigated. The major constituents were thymol (46.5%), carvacrol (31.7%), p-cymene (9%), and γ-terpinene (2.9%). To evaluate the toxicity of OEL in non-tumor cells, the hemolytic assay with Swiss mice erythrocytes was performed. The concentration producing 50% hemolysis (HC50) was 300 μg/mL. Sarcoma 180 tumor growth was inhibited in vivo 38% at 50 mg/kg, and 60% at 100 mg/kg, whereas 5-FU at 50 mg/kg caused 86% inhibition. OEL displays moderate gastrointestinal and hematological toxicity along with causing some alteration in liver function and morphology. However, the changes were considered reversible and negligible in comparison to the effects of several anticancer drugs. In summary, OEL displays in vivo antitumor activity and a moderate toxicity, which suggests further pharmacological study.

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Clinical Trial with 4-Hydroxy-17 α-methyl-19-nortestosterone in Advanced Breast Cancer

Tumori Journal ◽

10.1177/030089166405000602 ◽

1964 ◽

Vol 50 (6) ◽

pp. 445-456 ◽

Cited By ~ 3

Author(s):

Sergio Di Pietro ◽

Bruno Salvatori

Keyword(s):

Breast Cancer ◽

Clinical Trial ◽

Side Effects ◽

Antitumor Activity ◽

Advanced Breast Cancer ◽

Marked Effect ◽

Premenopausal Women ◽

Advanced Breast ◽

Clinical Feature ◽

Neoplastic Lesions

Twenty-two women with advanced breast cancer underwent clinical trial with a new hormonal compound, 4-hydroxy-17 α-methyl - 19 - nortestosterone (HMNT), in order to evaluate the antitumor activity of this steroid. The age of the patients ranged from 39 to 72 years; the compound was administrated i.m. at the dose of 50 mg a day, regularly and continuously, as long as a new progression of the neoplastic lesions was observed (in one case the treatment went on over a period of 13 months). An objective regression of the lesions was obtained in 8 of the 22 patients treated. The most impressive clinical feature of this new drug seems to consist in a more marked effect in premenopausal women, under 50 years of age. The compound proved to be practically free of virilizing action and of other unwanted side-effects, at least at the doses employed in this study.

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IN VIVO ANTITUMOR ACTIVITY OF PHYTOCHEMICAL PITC-2 OBTAINED FROM TISSUE CULTURED PLANT PLUCHEA INDICA ON SARCOMA-180 SOLID TUMOR MICE MODEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23968 ◽

2018 ◽

Vol 11 (4) ◽

pp. 211

Author(s):

Soumita Goswami ◽

Souvik Debnath ◽

Saumen Karan ◽

Tapan Kumar Chatterjee

Keyword(s):

Antitumor Activity ◽

Solid Tumor ◽

Sarcoma 180 ◽

Tumor Cell Proliferation ◽

Mice Model ◽

Ki 67 ◽

Cycle Arrest ◽

In Vivo Antitumor Activity ◽

G1 Cell Cycle Arrest

Objective: PITC-2 was isolated from the methanolic root extract of tissue cultured medicinal plant Pluchea indica (L.) Less. PITC-2 is a thiophene derivative which is 2-(Prop-1-ynyl)-5(5,6-dihydroxyhexa-1,3-diynyl)-thiophene. The main objective of the study is to evaluate the in vivo antitumor activity of PITC 2 against sarcoma-180 cancer cell in Swiss albino mice.Methods: The antitumor activity was evaluated by treatment with PITC-2 at a dose of 2.5 and 5 mg/kg b.w for 21 days on sarcoma-180 mice model. Cell viability was studied using 3-(4, 5- dimethylthiazol -2-yl)-2, 5-diphenyl tetrazolium bromide assay and cell apoptosis, G1 cell cycle arrest and reduction in tumor cell proliferation were evaluated by histopathological analysis and Bcl-2, cyclic-D1, and Ki-67 protein expression through immunohistochemistry study.Results: Precisely, PITC-2 had a cytotoxic effect on various in vitro cancer cells. Significant decreases in solid tumor volume and weight along with increase lifespan also observed. The histopathological and immunohistopathological examination indicates that PITC-2 induces apoptosis, typical morphological changes and suppresses tumor cell proliferation along with G1 cell cycle arrest through the downregulation of the intratumoral expression of Bcl-2, cyclic D1, and Ki-67 and thus highlighting antiproliferative and apoptotic properties against sarcoma-180 in vivo solid tumor model.Conclusion: The present results clearly demonstrate that PITC-2 significantly inhibits sarcoma-180 cell growth in a dose-dependent manner in in vivo mice model. Besides this, the study reveals a comprehensive perception of the possible mechanism behind the antitumor activity of PITC-2 by significant changes in the morphological, hematological, biochemical parameters in sarcoma-180 cells.

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Cancer Immunotherapy and Application of Nanoparticles in Cancers Immunotherapy as the Delivery of Immunotherapeutic Agents and as the Immunomodulators

Cancers ◽

10.3390/cancers12123773 ◽

2020 ◽

Vol 12 (12) ◽

pp. 3773

Author(s):

Tilahun Ayane Debele ◽

Cheng-Fa Yeh ◽

Wen-Pin Su

Keyword(s):

Survival Rate ◽

Side Effects ◽

Antitumor Activity ◽

Cancer Immunotherapy ◽

Cancer Patients ◽

Rapid Progress ◽

Therapeutic Targeting ◽

Alternative Methodology ◽

Stimuli Sensitive ◽

Field Area

In the last few decades, cancer immunotherapy becomes an important tactic for cancer treatment. However, some immunotherapy shows certain limitations including poor therapeutic targeting and unwanted side effects that hinder its use in clinics. Recently, several researchers are exploring an alternative methodology to overcome the above limitations. One of the emerging tracks in this field area is nano-immunotherapy which has gone through rapid progress and revealed considerable potentials to solve limitations related to immunotherapy. Targeted and stimuli-sensitive biocompatible nanoparticles (NPs) can be synthesized to deliver immunotherapeutic agents in their native conformations to the site of interest to enhance their antitumor activity and to enhance the survival rate of cancer patients. In this review, we have discussed cancer immunotherapy and the application of NPs in cancer immunotherapy, as a carrier of immunotherapeutic agents and as a direct immunomodulator.

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Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALB/c Mice

Advances in Materials Science and Engineering ◽

10.1155/2019/3534807 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Thi Thao Do ◽

Thi Phuong Do ◽

Thi Nga Nguyen ◽

Thi Cuc Nguyen ◽

Thi Thu Phuong Vu ◽

...

Keyword(s):

Side Effects ◽

Antitumor Activity ◽

Cytotoxic Activity ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lewis Lung ◽

Antitumor Activities ◽

Antitumor Effects ◽

Carcinoma Tumor

Although L-Asparaginase (L-ASP) is an effective chemotherapeutic agent, it has side effects such as fever, skin rashes, chills, anaphylaxis, and severe allergic reactions. Moreover, the short half-life of L-ASP reduces its antitumor activity. To reduce its side effects and broaden its pharmaceutical applications, L-ASP obtained from Pectobacterium carotovorum was subjected to liposomal conjugation. The enzyme was then loaded into liposomes using the hydrated thin-film method. The in vitro cytotoxic activity of liposomal L-ASP was evaluated with the MTT assay using cancerous cell lines, and its antitumor effects were examined in Lewis lung carcinoma (LLC) tumorized mice. The average size of the liposomes containing purified L-asparagine was 93.03 ± 0.49 nm. They had a zeta potential of –15.45 ± 6.72 mV, polydispersity index of 0.22 ± 0.02, and encapsulation efficiency of 53.99 ± 5.44%. The in vitro cytotoxic activity of liposomal L-ASP was less effective against LLC, MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), SK-LU-1 (human lung carcinoma), and NTERA-2 (pluripotent human embryonic carcinoma) cells than that of free L-ASP. However, the antitumor activity of liposomal L-ASP was significantly greater than that of untrapped L-ASP at the same doses (6 UI/mouse) in terms of tumor size (6309.11 ± 414.06 mm3) and life span (35.00 ± 1.12 days). This is the first time the antitumor activities of PEGylated nanoliposomal L-ASP have been assessed in LLC carcinoma tumor-induced BALB/c mice and showed significantly improved pharmacological properties compared to those of free L-ASP (P<0.05). Thus, nanoliposomal L-ASP should be considered for its widening applications against carcinoma tumors.

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Antitumor Activity of the Carboplatin-α-Cyclodextrin Adduct

Metal-Based Drugs ◽

10.1155/mbd.1997.305 ◽

1997 ◽

Vol 4 (6) ◽

pp. 305-306

Author(s):

Weiping Liu ◽

Huizhou Xiong ◽

Yikun Yang ◽

Ling Li ◽

Zhiqiang Shen ◽

...

Keyword(s):

Antitumor Activity ◽

Ehrlich Ascites Carcinoma ◽

Antitumor Efficacy ◽

Sarcoma 180 ◽

Murine Tumors ◽

Ehrlich Ascites

Antitumor activity of the adduct between Carboplatin and α-cyclodextrin has been tested against Sarcoma 180 (S180) and Ehrlich ascites carcinoma (EAC) murine tumors. The preliminary toxicity has also been evaluated by histological examinations of the treated animals. The results show the adduct has less antitumor efficacy than and similar toxicity to Carboplatin.

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