GC/MS analysis and anti-inflammatory effect of leaf extracts from Hibiscus syriacus through inhibition of NF-κB and MAPKs signaling in LPS-stimulated RAW264.7 macrophages

2020 ◽  
Vol 14 (5) ◽  
pp. 539-546
Author(s):  
Hyun Ji Eo ◽  
Hae Yun Kwon ◽  
Som Da Kim ◽  
Yeongyeong Kang ◽  
Youngki Park ◽  
...  
Keyword(s):  
Leaf Extracts ◽  
Raw264.7 Macrophages ◽  
Ms Analysis ◽  
Anti Inflammatory ◽  
Hibiscus Syriacus ◽  
Inflammatory Effect

scholarly journals Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ewelina Szliszka ◽  
Anna Mertas ◽  
Zenon P. Czuba ◽  
Wojciech Król
Keyword(s):  
Cell Viability ◽  
Radical Scavenging ◽  
Gm Csf ◽  
Raw264.7 Macrophages ◽  
Griess Reaction ◽  
Artepillin C ◽  
Anti Inflammatory ◽  
Radical Scavenging Ability ◽  
Brazilian Green Propolis ◽  
Inflammatory Effect

Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis. The aim of this study was to investigate the anti-inflammatory effect of artepillin C on LPS + IFN-γ- or PMA-stimulated RAW264.7 macrophages. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH•and ABTS•+. ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated RAW264.7 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. NF-κB activity was confirmed by the ELISA-based TransAM NF-κB kit. At the tested concentrations, the compound did not decrease the cell viability and did not cause the cytotoxicity. Artepillin C exerted strong antioxidant activity, significantly inhibited the production of ROS, RNS, NO, and cytokine IL-1β, IL-3, IL-4, IL-5, IL-9, IL-12p40, IL-13, IL-17, TNF-α, G-CSF, GM-CSF, MCP-1, MIP-1α, MIP-1β, RANTES, and KC, and markedly blocked NF-κB expression in stimulated RAW264.7 macrophages. Our findings provide new insights for understanding the mechanism involved in the anti-inflammatory effect of artepillin C and support the application of Brazilian green propolis in complementary and alternative medicine.

Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW264.7 macrophages

2006 ◽  
Vol 106 (3) ◽  
pp. 364-371 ◽  
Author(s):  
Byung-Chul Kim ◽  
Joung-Woo Choi ◽  
Hye-Young Hong ◽  
Sin-Ae Lee ◽  
Suntaek Hong ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Phellinus Linteus ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals PLA-PEG Nanoparticles Improve the Anti-Inflammatory Effect of Rosiglitazone on Macrophages by Enhancing Drug Uptake Compared to Free Rosiglitazone

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1845 ◽  
Author(s):  
Giovanna Giacalone ◽  
Nicolas Tsapis ◽  
Ludivine Mousnier ◽  
Hélène Chacun ◽  
Elias Fattal
Keyword(s):  
Polymeric Nanoparticles ◽  
Heart Diseases ◽  
The United States ◽  
Drug Uptake ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Efficient Delivery ◽  
Anti Inflammatory ◽  
Atherosclerosis Treatment ◽  
Inflammatory Effect

Among cardiovascular diseases, atherosclerosis remains the first cause of death in the United States of America and Europe, as it leads to myocardial infarction or stroke. The high prevalence of heart diseases is due to the difficulty in diagnosing atherosclerosis, since it can develop for decades before symptoms occur, and to the complexity of the treatment since targets are also important components of the host defenses. The antidiabetics thiazolidinediones, among which is rosiglitazone (RSG), have demonstrated anti-atherosclerotic effect in animal models, and are therefore promising candidates for the improvement of atherosclerosis management. Nevertheless, their administration is hindered by the insurgence of severe side effects. To overcome this limitation, rosiglitazone has been encapsulated into polymeric nanoparticles, which permit efficient delivery to its nuclear target, and selective delivery to the site of action, allowing the reduction of unwanted effects. In the present work, we describe nanoparticle formulation using polylactic acid (PLA) coupled to polyethylene glycol (PEG), their characterization, and their behavior on RAW264.7 macrophages, an important target in atherosclerosis treatment. RSG nanocarriers showed no toxicity on cells at all concentrations tested, an anti-inflammatory effect in a dose-dependent manner, up to 5 times more efficient than the free molecule, and an increased RSG uptake which is consistent with the effect shown. These biodegradable nanoparticles represent a valid tool to be further investigated for the treatment of atherosclerosis.

The Anti-inflammatory Effect of Polyphenolics Olive Leaf Extracts Olea europaea L.

2021 ◽  
Author(s):  
Y. Aissaoui ◽  
Y. Boukhari ◽  
M. A. Mahi
Keyword(s):  
Olea Europaea ◽  
Acute Inflammation ◽  
Inflammatory Activity ◽  
Olive Leaves ◽  
Leaf Extracts ◽  
Polyphenolic Extract ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Inflammatory Effect ◽  
Olea Europaéa

This study was to evaluate the anti-inflammatory effect of the polyphenolic extract of olive leaves Olea europaea L. In vivo experiments were performed on the model of acute inflammation edema of the paw of male Wistar rats following inflammation induced by the injection of carrageenan. The anti-inflammatory effect is carried out by the injection of the polyphenolic extracts of olive leaves Olea europaea L. at different doses 50 mg/kg, 100 mg/kg and 150 mg/kg, the injection was carried out 30 min before induction of acute inflammation with carrageenan at 1%. The results obtained were compared with those of Diclofenac and those of the physiological control. Injection of carrageenan resulted in a significant increase in the claw leg volume of 46.24 ± 23.8%, 66.78 ± 29.69% and 90.31 ± 46.51%, respectively at 1 h, 2 h, and 3 h. The results showed that the inflammatory activity of the polyphenolic extracts of olive leaves was dose dependent. The 150 mg/kg Olea europaea L. extract of polyphenolic has an anti-inflammatory activity which results in an 88% decrease in edema. This decrease is significantly similar to that of Diclofenac® 97% confirmed that the polyphenolic extracts of Olea europaea L. leaves have anti-inflammatory activities comparable to that of Diclofenac® with a non-significant difference (p < 0.05), this anti-inflammatory effect remains lower than that of Diclofenac®.

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