The Efficacy of Stem Cells Secretome Application in Osteoarthritis: A Systematic Review of In Vivo Studies

Cell-based therapy is a promising treatment to favor tissue healing through less invasive strategies. Mesenchymal stem cells (MSCs) highlighted as potential candidates due to their angiogenic, anti-apoptotic and immunomodulatory properties, in addition to their ability to differentiate into several specialized cell lines. Cells can be carried through a biological delivery system, such as fibrin glue, which acts as a temporary matrix that favors cell-matrix interactions and allows local and paracrine functions of MSCs. Thus, the aim of this systematic review was to evaluate the potential of fibrin glue combined with MSCs in nerve regeneration. The bibliographic search was performed in the PubMed/MEDLINE, Web of Science and Embase databases, using the descriptors (“fibrin sealant” OR “fibrin glue”) AND “stem cells” AND “nerve regeneration”, considering articles published until 2021. To compose this review, 13 in vivo studies were selected, according to the eligibility criteria. MSCs favored axonal regeneration, remyelination of nerve fibers, as well as promoted an increase in the number of myelinated fibers, myelin sheath thickness, number of axons and expression of growth factors, with significant improvement in motor function recovery. This systematic review showed clear evidence that fibrin glue combined with MSCs has the potential to regenerate nervous system lesions.

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Therapeutic efficiency of bone marrow-derived mesenchymal stem cells for liver fibrosis: A systematic review of in vivo studies

World Journal of Gastroenterology ◽

10.3748/wjg.v26.i47.7444 ◽

2020 ◽

Vol 26 (47) ◽

pp. 7444-7469

Author(s):

Zaid Al-Dhamin ◽

Ling-Di Liu ◽

Dong-Dong Li ◽

Si-Yu Zhang ◽

Shi-Ming Dong ◽

...

Keyword(s):

Systematic Review ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Liver Fibrosis ◽

In Vivo Studies ◽

Therapeutic Efficiency

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Transplantation of Adipose-derived Cells for Periodontal Regeneration: A Systematic Review

Current Stem Cell Research & Therapy ◽

10.2174/1574888x13666181105144430 ◽

2019 ◽

Vol 14 (6) ◽

pp. 504-518 ◽

Cited By ~ 3

Author(s):

Dilcele Silva Moreira Dziedzic ◽

Bassam Felipe Mogharbel ◽

Priscila Elias Ferreira ◽

Ana Carolina Irioda ◽

Katherine Athayde Teixeira de Carvalho

Keyword(s):

Systematic Review ◽

Animal Models ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

In Vitro Studies ◽

In Vivo Studies ◽

Cell Sources ◽

Study Designs

This systematic review evaluated the transplantation of cells derived from adipose tissue for applications in dentistry. SCOPUS, PUBMED and LILACS databases were searched for in vitro studies and pre-clinical animal model studies using the keywords “ADIPOSE”, “CELLS”, and “PERIODONTAL”, with the Boolean operator “AND”. A total of 160 titles and abstracts were identified, and 29 publications met the inclusion criteria, 14 in vitro and 15 in vivo studies. In vitro studies demonstrated that adipose- derived cells stimulate neovascularization, have osteogenic and odontogenic potential; besides adhesion, proliferation and differentiation on probable cell carriers. Preclinical studies described improvement of bone and periodontal healing with the association of adipose-derived cells and the carrier materials tested: Platelet Rich Plasma, Fibrin, Collagen and Synthetic polymer. There is evidence from the current in vitro and in vivo data indicating that adipose-derived cells may contribute to bone and periodontal regeneration. The small quantity of studies and the large variation on study designs, from animal models, cell sources and defect morphology, did not favor a meta-analysis. Additional studies need to be conducted to investigate the regeneration variability and the mechanisms of cell participation in the processes. An overview of animal models, cell sources, and scaffolds, as well as new perspectives are provided for future bone and periodontal regeneration study designs.

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Current status of wet lab and cadaveric simulation in urological training: A systematic review

Canadian Urological Association Journal ◽

10.5489/cuaj.6520 ◽

2020 ◽

Vol 14 (11) ◽

Author(s):

Ahmed Al-Jabir ◽

Abdullatif Aydin ◽

Hussain Al-Jabir ◽

M. Shamim Khan ◽

Prokar Dasgupta ◽

...

Keyword(s):

Systematic Review ◽

English Language ◽

In Vivo Studies ◽

Current Status ◽

Level Of Evidence ◽

Cadaveric Simulation ◽

Randomized Controlled Studies ◽

Wet Lab ◽

Urological Training

Introduction: We undertook a systematic review of the use of wet lab (animal and cadaveric) simulation models in urological training, with an aim to establishing a level of evidence (LoE) for studies and level of recommendation (LoR) for models, as well as evaluating types of validation. Methods: Medline, EMBASE, and Cochrane databases were searched for English-language studies using search terms including a combination of surgery, surgical training, and medical education. These results were combined with wet lab, animal model, cadaveric, and in-vivo. Studies were then assigned a LoE and LoR if appropriate as per the education-modified Oxford Centre for Evidence-Based Medicine classification. Results: A total of 43 articles met the inclusion criteria. There was a mean of 23.1 (±19.2) participants per study with a median of 20. Overall, the studies were largely of low quality, with 90.7% of studies being lower than 2a LoE (n=26 for LoE 2b and n=13 for LoE 3). The majority (72.1%, n=31) of studies were in animal models and 27.9% (n=12) were in cadaveric models. Conclusions: Simulation in urological education is becoming more prevalent in the literature, however, there is a focus on animal rather than cadaveric simulation, possibly due to cost and ethical considerations. Studies are also predominately of a low LoE; more higher LoEs, especially randomized controlled studies, are needed.

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Regenerative potential of secretome from dental stem cells: a systematic review of preclinical studies

Reviews in the Neurosciences ◽

10.1515/revneuro-2017-0069 ◽

2018 ◽

Vol 29 (3) ◽

pp. 321-332 ◽

Cited By ~ 7

Author(s):

Suleiman Alhaji Muhammad ◽

Norshariza Nordin ◽

Sharida Fakurazi

Keyword(s):

Systematic Review ◽

Stem Cells ◽

Stem Cell ◽

Animal Models ◽

Conditioned Medium ◽

Tissue Regeneration ◽

Therapeutic Benefit ◽

Risk Of Bias ◽

Preclinical Studies

AbstractInjury to tissues is a major clinical challenge due to the limited regenerative capacity of endogenous cells. Stem cell therapy is evolving rapidly as an alternative for tissue regeneration. However, increasing evidence suggests that the regenerative ability of stem cells is mainly mediated by paracrine actions of secretome that are generally secreted by the cells. We aimed to systematically evaluate the efficacy of dental stem cell (DSC)-conditioned medium inin vivoanimal models of various tissue defects. A total of 15 eligible studies was included by searching Pubmed, Scopus and Medline databases up to August 2017. The risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation risk of bias tool. Of 15 studies, seven reported the therapeutic benefit of the conditioned medium on neurological diseases and three reported on joint/bone-related defects. Two interventions were on liver diseases, whereas the remaining three addressed myocardial infarction and reperfusion, lung injury and diabetes. Nine studies were performed using mouse models and the remaining six studies used rat models. The methodological quality of the studies was low, as most of the key elements required in reports of preclinical studies were not reported. The findings of this review suggested that conditioned medium from DSCs improved tissue regeneration and functional recovery. This current review strengthens the therapeutic benefit of cell-free product for tissue repair in animal models. A well-planned study utilizing validated outcome measures and long-term safety studies are required for possible translation to clinical trials.

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Antiproliferative activity of thiazole and oxazole derivatives: A systematic review of in vitro and in vivo studies

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2021.111495 ◽

2021 ◽

Vol 138 ◽

pp. 111495

Author(s):

Nancy Y. Guerrero-Pepinosa ◽

María C. Cardona-Trujillo ◽

Sandra C. Garzón-Castaño ◽

Luz Angela Veloza ◽

Juan C. Sepúlveda-Arias

Keyword(s):

Systematic Review ◽

Antiproliferative Activity ◽

In Vivo Studies ◽

Oxazole Derivatives

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The Act of Controlling Adult Stem Cell Dynamics: Insights from Animal Models

Biomolecules ◽

10.3390/biom11050667 ◽

2021 ◽

Vol 11 (5) ◽

pp. 667

Author(s):

Meera Krishnan ◽

Sahil Kumar ◽

Luis Johnson Kangale ◽

Eric Ghigo ◽

Prasad Abnave

Keyword(s):

Stem Cells ◽

Animal Models ◽

Adult Stem Cells ◽

The Body ◽

In Vivo Studies ◽

Self Renewal ◽

Hematopoietic Stem ◽

Organ Systems

Adult stem cells (ASCs) are the undifferentiated cells that possess self-renewal and differentiation abilities. They are present in all major organ systems of the body and are uniquely reserved there during development for tissue maintenance during homeostasis, injury, and infection. They do so by promptly modulating the dynamics of proliferation, differentiation, survival, and migration. Any imbalance in these processes may result in regeneration failure or developing cancer. Hence, the dynamics of these various behaviors of ASCs need to always be precisely controlled. Several genetic and epigenetic factors have been demonstrated to be involved in tightly regulating the proliferation, differentiation, and self-renewal of ASCs. Understanding these mechanisms is of great importance, given the role of stem cells in regenerative medicine. Investigations on various animal models have played a significant part in enriching our knowledge and giving In Vivo in-sight into such ASCs regulatory mechanisms. In this review, we have discussed the recent In Vivo studies demonstrating the role of various genetic factors in regulating dynamics of different ASCs viz. intestinal stem cells (ISCs), neural stem cells (NSCs), hematopoietic stem cells (HSCs), and epidermal stem cells (Ep-SCs).

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TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

Biomedical Engineering Applications Basis and Communications ◽

10.4015/s1016237209001209 ◽

2009 ◽

Vol 21 (03) ◽

pp. 149-155 ◽

Cited By ~ 2

Author(s):

Hsu-Wei Fang

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Growth Factors ◽

Bone Cells ◽

Cartilage Tissue Engineering ◽

Bioactive Molecules ◽

In Vivo Studies ◽

Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

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Fatty acid is a potential agent for bone tissue induction: In vitro and in vivo approach

Experimental Biology and Medicine ◽

10.1177/1535370217731104 ◽

2017 ◽

Vol 242 (18) ◽

pp. 1765-1771 ◽

Cited By ~ 6

Author(s):

Guinea BC Cardoso ◽

Erivelto Chacon ◽

Priscila GL Chacon ◽

Pedro Bordeaux-Rego ◽

Adriana SS Duarte ◽

...

Keyword(s):

Stem Cells ◽

Oleic Acid ◽

Fatty Acid ◽

Bone Tissue ◽

In Vivo Studies ◽

Surrounding Tissue ◽

Bone Induction ◽

Acid Addition

Our hypothesis was to investigate the fatty acid potential as a bone induction factor. In vitro and in vivo studies were performed to evaluate this approach. Oleic acid was used in a 0.5 wt.% concentration. Polycaprolactone was used as the polymeric matrix by combining solvent-casting and particulate-leaching techniques, with a final porosity of 70 wt.%, investigated by SEM images. Contact angle measurements were produced to investigate the influence of oleic acid on polycaprolactone chains. Cell culture was performed using adipocyte-derived stem cells to evaluate biocompatibility and bioactivity properties. In addition, in vivo studies were performed to evaluate the induction potential of oleic acid addition. Adipocyte-derived stem cells were used to provide differentiation after 21 days of culture. Likewise, information were obtained with in vivo data and cellular invagination was observed on both scaffolds (polycaprolactone and polycaprolactone /oleic acid); interestingly, the scaffold with oleic acid addition demonstrated that cellular migrations are not related to the surrounding tissue, indicating bioactive potential. Our hypothesis is that fatty acid may be used as a potential induction factor for bone tissue engineering. The study’s findings indicate oleic acid as a possible agent for bone induction, according to data on cell differentiation, proliferation, and migration. Impact statement The biomaterial combined in this study on bone regeneration is innovative and shows promising results in the treatment of bone lesions. Polycaprolactone (PCL) and oleic acid have been studied separately. In this research, we combined biomaterials to assess the stimulus and the speed of bone healing.

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