Analysis of embryo, cytoplasmic and maternal genetic correlations for seven essential amino acids in rapeseed meal (Brassica napus L.)

2011 ◽  
Vol 90 (1) ◽  
pp. 67-74 ◽  
Author(s):  
GUO LIN CHEN ◽  
JIAN GUO WU ◽  
MURALI-TOTTEKKAAD VARIATH ◽  
ZHONG WEI YANG ◽  
CHUN HAI SHI
1975 ◽  
Vol 55 (1) ◽  
pp. 61-70 ◽  
Author(s):  
J. M. BELL

Five swine experiments were conducted to evaluate rapeseed meal (RSM) of low glucosinolate content (Brassica napus L. cv. Bronowski). Two experiments involved 0, 25, 50, 75 and 100% substitution of either Bronowski meal or regular (B. campestris) meal for soybean meal or fishmeal used in the control diet. One experiment compared ad libitum-fed and partially restricted pigs. Another experiment involved digestibility studies, and the final one involved methionine and lysine supplementation. As the dietary levels of either Bronowski or regular RSM increased in the ration, protein digestion coefficients decreased from 79 and 80% to 76 and 78%, respectively, and energy coefficients decreased from 82% to 79 and 78%, respectively. The protein and energy digestibility coefficients for Bronowski RSM were estimated to be 68 and 59%; for regular RSM, 65 and 54%. With barley–wheat–RSM diets, pigs responded to 0.1% methionine, but not to lysine (P > 0.05). Pigs fed ad libitum consumed more Bronowski than regular RSM diet and performed as well as pigs fed soybean meal diets.


1987 ◽  
Vol 67 (3) ◽  
pp. 545-559 ◽  
Author(s):  
W. F. NUTTALL ◽  
H. UKRAINETZ ◽  
J. W. B. STEWART ◽  
D. T. SPURR

In northeastern Saskatchewan on Gray Luvisolic soils, rapeseed (Brassica napus L. and B. campestris L.) grown on many fields does not set seed, possibly because of deficiencies of S and B. Therefore, experiments were begun in 1979 to determine (1) the effect of N, S and B fertilizers on yield and quality of rapeseed; (2) if cultivars (B. napus and B. campestris L.) responded differently to these nutrients; and (3) nutrient and nutrient interaction effects of five rates of N, S and B in a composite rotatable design on yield and quality of the cultivar Regent (B. napus L.). Rates of up to 200 kg N ha−1, 50 kg S ha−1 and 2.8 kg B ha−1 were applied. The experiments were conducted on 13 sites. Nine were in N.E. Saskatchewan on Sylvania f1, Waitville 1 (Luvisolic) and Melfort sicl (Black Chernozemic) soils. Four were in N.W. Saskatchewan on Loon River 1 and Waitville 1, (Luvisolic) soils. In N.W. Saskatchewan there was a significant yield increase because of N (1.00 t ha−1) and S (1.06 t ha−1). In N.E. Saskatchewan on Sylvania f1, rapeseed yields were increased by 0.38 t ha−1 by a combination of S and B and by 0.78 t ha−1 by N. Sylvania f1 soils were lower in soluble B than other experimental sites. At other sites in N.E. Saskatchewan, N but not S increased rapeseed grain yield significantly. Significant response to a combination of S and B was obtained with the cultivar Regent, and both species of rapeseed responded to S fertilizer. Sulphur fertilizer increased the glucosinolate concentration in rapeseed meal at all sites. Sulphur increased oil concentration of rapeseed on all sites except one where frost damaged the crop and increased protein of grain on sites where there was yield response to S. Nitrogen increased protein of rapeseed grown on all sites whereas N combined with B decreased protein and increased oil percentage on all sites except Sylvania f1. The yield response of the cultivar Regent to B was not significantly related to soluble soil B. The combined yield response to S and B in relation to soluble soil S and B was significant (R2 = 0.60). Yield response of rapeseed to S was significantly related to soluble soil S (R2 = 0.35). In conclusion, S fertilizer solved the problem of poor seed set in rapeseed cultivars, but B also enhanced yield by decreasing the number of sterile florets and improving pod development. Key words: Nitrogen, sulphur, boron, rapeseed, oil, protein, glucosinolates


1969 ◽  
Vol 47 (5) ◽  
pp. 679-685 ◽  
Author(s):  
A. J. Finlayson ◽  
R. S. Bhatty ◽  
C. M. Christ

Two proteins, previously described by the authors as BI (S020, buffer 12 S) and AIVS (S020, w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.


1972 ◽  
Vol 126 (4) ◽  
pp. 985-991 ◽  
Author(s):  
T. T. Ngo ◽  
P. D. Shargool

Sodium [35S]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label subsequently appeared in cystathionine and methionine. The results obtained indicated that both the sulphydration and trans-sulphuration pathways were operating. This conclusion was reinforced by the results of experiments in which batches of rapeseed were incubated with l-[14C]homoserine. These showed the formation of labelled homocysteine, cystathione and methionine. It was thought the trans-sulphuration pathway was making the greater contribution to the biosynthesis of methionine in germinating rapeseed.


1966 ◽  
Vol 44 (7) ◽  
pp. 997-1004 ◽  
Author(s):  
A. J. Finlayson

Four groups of five rapeseed plants (Brassica napus L.) were injected with sodium acetate-2-14C at intervals between flowering and maturity. Two 14C-labeled proteins were isolated from a water extract of the oil-free seed meal. The specific activities of the two proteins and of some amino acids isolated from them suggested rate differences in protein synthesis, or, alternatively, conversion of one protein into another. The results also showed that synthesis of these proteins occurs over a considerable period in the plant's development.


1967 ◽  
Vol 45 (17) ◽  
pp. 1971-1974 ◽  
Author(s):  
M. E. Daxenbichler ◽  
C. H. VanEtten ◽  
W. H. Tallent ◽  
I. A. Wolff

When defatted seed from Brassica napus L. var. Regina II (a rapeseed) was autolyzed, its major thioglucoside (progoitrin) underwent degradation analogous to that for epiprogoitrin in Crambe abyssinica seed meal. Depending on the conditions, (R)-1-cyano-2-hydroxy-3-butene and the diastereomeric forms of (2R)-1-cyano-2-hydroxy-3,4-epithiobutane were formed in autolyzed B. napus meal instead of (S)-goitrin. The configuration at the chiral center containing the hydroxyl is assigned on the basis of known data. The configuration at carbon 3 of the episulfides is predicted on the basis of the optical rotatory dispersion data.


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