scholarly journals Genome-wide identification and functional prediction of salt- stress related long non-coding RNAs (lncRNAs) in chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Neeraj Kumar ◽  
Chellapilla Bharadwaj ◽  
Sarika Sahu ◽  
Aalok Shiv ◽  
Abhishek Kumar Shrivastava ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Target Genes ◽  
Salt Stress Response ◽  
Rna Molecules ◽  
Transporter Family ◽  
Genome Wide ◽  
New Generation ◽  
In Cis

AbstractLncRNAs (long noncoding RNAs) are 200 bp length crucial RNA molecules, lacking coding potential and having important roles in regulating gene expression, particularly in response to abiotic stresses. In this study, we identified salt stress-induced lncRNAs in chickpea roots and predicted their intricate regulatory roles. A total of 3452 novel lncRNAs were identified to be distributed across all 08 chickpea chromosomes. On comparing salt-tolerant (ICCV 10, JG 11) and salt-sensitive cultivars (DCP 92–3, Pusa 256), 4446 differentially expressed lncRNAs were detected under various salt  treatments. We predicted 3373 lncRNAs to be regulating their target genes in cis regulating manner and 80 unique lncRNAs were observed as interacting with 136 different miRNAs, as eTMs (endogenous target mimic) targets of miRNAs and implicated them in the regulatory network of salt stress response. Functional analysis of these lncRNA revealed their association in targeting salt stress response-related genes like potassium transporter, transporter family genes, serine/threonine-protein kinase, aquaporins like TIP1-2, PIP2-5 and transcription factors like, AP2, NAC, bZIP, ERF, MYB and WRKY. Furthermore, about 614 lncRNA-SSRs (simple sequence repeats) were identified as a new generation of molecular markers with higher efficiency and specificity in chickpea. Overall, these findings will pave the understanding of comprehensive functional role of potential lncRNAs, which can help in providing insight into the molecular mechanism of salt tolerance in chickpea.

Molecular insights into the role of plant transporters in salt stress response

2021 ◽  
Author(s):  
Ashok Saddhe Ankush ◽  
Ajay Kumar Mishra ◽  
Kumar Kundan
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response

Phenotypic plasticity with respect to salt stress response by Lotus glaber: the role of its AM fungal and rhizobial symbionts

Mycorrhiza ◽  
2008 ◽  
Vol 18 (6-7) ◽  
pp. 317-329 ◽  
Author(s):  
Mariela Echeverria ◽  
Agustina Azul Scambato ◽  
Analía Inés Sannazzaro ◽  
Santiago Maiale ◽  
Oscar Adolfo Ruiz ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Phenotypic Plasticity ◽  
Salt Stress Response ◽  
Lotus Glaber

Quantitative proteomics reveals an important role of GsCBRLK in salt stress response of soybean

2015 ◽  
Vol 402 (1-2) ◽  
pp. 159-178 ◽  
Author(s):  
Wei Ji ◽  
Jin Koh ◽  
Sheng Li ◽  
Ning Zhu ◽  
Craig P. Dufresne ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Quantitative Proteomics ◽  
Salt Stress Response

scholarly journals Molecular Characterization of NDL1-AGB1 Mediated Salt Stress Signaling: Further Exploration of the Role of NDL1 Interacting Partners

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2261
Author(s):  
Nidhi Gupta ◽  
Abhishek Kanojia ◽  
Arpana Katiyar ◽  
Yashwanti Mudgil
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
G Protein ◽  
Salinity Stress ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Negative Regulator ◽  
Salt Stress Response

Salt stress is considered to be the most severe abiotic stress. High soil salinity leads to osmotic and ionic toxicity, resulting in reduced plant growth and crop production. The role of G-proteins during salt stresses is well established. AGB1, a G-protein subunit, not only plays an important role during regulation of Na+ fluxes in roots, but is also involved in the translocation of Na+ from roots to shoots. N-Myc Downregulated like 1 (NDL1) is an interacting partner of G protein βγ subunits and C-4 domain of RGS1 in Arabidopsis. Our recent in-planta expression analysis of NDL1 reported changes in patterns during salt stress. Based on these expression profiles, we have carried out functional characterization of the AGB1-NDL1 module during salinity stress. Using various available mutant and overexpression lines of NDL1 and AGB1, we found that NDL1 acts as a negative regulator during salt stress response at the seedling stage, an opposite response to that of AGB1. On the other hand, during the germination phase of the plant, this role is reversed, indicating developmental and tissue specific regulation. To elucidate the mechanism of the AGB1-NDL1 module, we investigated the possible role of the three NDL1 stress specific interactors, namely ANNAT1, SLT1, and IDH-V, using yeast as a model. The present study revealed that NDL1 acts as a modulator of salt stress response, wherein it can have both positive as well as negative functions during salinity stress. Our findings suggest that the NDL1 mediated stress response depends on its developmental stage-specific expression patterns as well as the differential presence and interaction of the stress-specific interactors.

The role of antioxidative metabolism of tomato leaves in long-term salt-stress response

2017 ◽  
Vol 180 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Ivana Koleška ◽  
Dino Hasanagić ◽  
Ivana Maksimović ◽  
Borut Bosančić ◽  
Biljana Kukavica
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response ◽  
Antioxidative Metabolism

scholarly journals Genome-wide identification of Thellungiella salsuginea microRNAs with putative roles in the salt stress response

2013 ◽  
Vol 13 (1) ◽  
pp. 180 ◽  
Author(s):  
Quan Zhang ◽  
Chuanzhi Zhao ◽  
Ming Li ◽  
Wei Sun ◽  
Yan Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Thellungiella Salsuginea ◽  
Salt Stress Response ◽  
Genome Wide

scholarly journals AhABI4s Negatively Regulate Salt-Stress Response in Peanut

2021 ◽  
Vol 12 ◽  
Author(s):  
Lu Luo ◽  
Qian Wan ◽  
Kun Zhang ◽  
Xiurong Zhang ◽  
Ruijie Guo ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Target Genes ◽  
Yield Potential ◽  
Regulation Mechanism ◽  
Phosphorylation Sites ◽  
Mobility Shift ◽  
Salt Stress Response ◽  
Binding Preference

Soil salinity is one of the major factors that limit the area of cultivable land and yield potential of crops. The ability of salt tolerance varies with plant species. Peanut (Arachis hypogaea L.) is a moderately salt-sensitive and economically important crop, however, their biological processes involved in salt-stress response remain unclear. In this study, we investigated the role of A. hypogaea L. ABSCISIC ACID INSENSITIVE 4s (AhABI4s) in salt tolerance and elucidated its mode of action in peanuts. The results showed that the downregulation of AhABI4s via whole plant virus-induced gene silencing has enhanced the survival rate, biomass accumulation, and root/shoot ratio of peanut seedlings in response to salt-stress. Transcriptomics, quantitative proteomics, and phosphoproteomic analyses were performed using AhABI4s-silenced and Mock plants. The expression pattern of 15,247 genes, 1,900 proteins, and 2,620 phosphorylation sites were affected by silencing of AhABI4s in peanut leaf and root after sodium chloride (NaCl) treatment. Among them, 63 potential downstream target genes of ABI4 changed consistently at both transcription and translation levels, and the protein/phosphorylation levels of 31 ion transporters/channels were also affected. Electrophoretic mobility shift assays (EMSA) showed that ABI4 was able to bind to the promoters of HSP70, fructokinase (FRK), and pyruvate kinase (PK) coding genes in vitro. In addition, we also detected a binding preference of AhABI4 for CACT(G/T)GCA motif in the promoters of down-regulated genes in peanut leaf. Collectively, the potential downstream targets which were regulated at the levels of transcription and translation, binding preference, and in vivo phosphorylation sites that had been revealed in this study will provide new insight into the AhABI4s-mediated salt tolerance regulation mechanism in peanuts.

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