Molecular Detection for Two Abalone Shell-boring Species Polydora haswelli and P. hoplura (Polychaeta, Spionidae) from Korea using 18S rDNA and cox1 Markers

Abstract Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to “Candidatus Rickettsia colombianensi”, a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.

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Comparison of heat shock protein 70 kDa and 18S rDNA genes for molecular detection and phylogenetic analysis of Babesia vogeli from whole blood of naturally infected dogs

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2018.01.013 ◽

2018 ◽

Vol 9 (3) ◽

pp. 556-562 ◽

Cited By ~ 2

Author(s):

Patrícia G. Paulino ◽

Marcus S. Pires ◽

Claudia B. da Silva ◽

Maristela Peckle ◽

Renata L. da Costa ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Heat Shock ◽

Heat Shock Protein ◽

Whole Blood ◽

18S Rdna ◽

Molecular Detection ◽

Rdna Genes ◽

Babesia Vogeli

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MOLECULAR DETECTION OF RANGELIA VITALII IN A DOG FROM THE CURITIBA METROPOLITAN REGION, PARANA STATE, SOUTHERN BRAZIL

Archives of Veterinary Science ◽

10.5380/avs.v22i4.56936 ◽

2017 ◽

Vol 22 (4) ◽

Author(s):

Anna C.B. Mongruel ◽

Simone Tostes de Oileira Stedile ◽

Marlos G Sousa ◽

Jessica DM Valente ◽

Iago C Barbosa ◽

...

Keyword(s):

Weight Loss ◽

18S Rdna ◽

Molecular Detection ◽

Clinical Care ◽

Positive Sample ◽

Metropolitan Region ◽

Southern Brazil ◽

Intermittent Fever ◽

Mixed Breed ◽

18S Rdna Sequence

Rangeliosis, caused by Rangelia vitalii, is known to causes apathy, intermittent fever, ear’s margin bleeding and anemia. Herein, we report the first molecular detection of R. vitalii infecting a dog from the Curitiba metropolitan region, Paraná State, southern Brazil. A four-year-old male mixed-breed neighborhood dog, presenting apathy, weight loss, and ear’s margin bleeding, was referred for clinical care at the Veterinary Teaching Hospital of the Universidade Federal do Paraná. Sequencing of the piroplasm positive sample showed ≥99% identity with R. vitalii 18S rDNA sequence deposited in GenBank. Further studies are needed to elucidate the pathogenicity, competence vector of ticks and epidemiology of this protozoa the State of Paraná.

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Molecular characterization of putative Hepatozoon sp. from the sedge warbler (Acrocephalus schoenobaenus)

Parasitology ◽

10.1017/s0031182012002004 ◽

2013 ◽

Vol 140 (6) ◽

pp. 695-698 ◽

Cited By ~ 11

Author(s):

ALEKSANDRA BIEDRZYCKA ◽

AGNIESZKA KLOCH ◽

MAGDALENA MIGALSKA ◽

WOJCIECH BIELAŃSKI

Keyword(s):

Molecular Characterization ◽

18S Rdna ◽

Molecular Detection ◽

Phylogenetic Analyses ◽

Systematic Position ◽

The Third ◽

Sedge Warbler ◽

Blood Smears

SUMMARYWe characterized partial sequences of 18S rDNA from sedge warblers infected with a parasite described previously as Hepatozoon kabeeni. Prevalence was 47% in sampled birds. We detected 3 parasite haplotypes in 62 sequenced samples from infected animals. In phylogenetic analyses, 2 of the putative Hepatozoon haplotypes closely resembled Lankesterella minima and L. valsainensis. The third haplotype grouped in a wider clade composed of Caryospora and Eimeria. None of the haplotypes showed resemblance to sequences of Hepatozoon from reptiles and mammals. Molecular detection results were consistent with those from microscopy of stained blood smears, confirming that the primers indeed amplified the parasite sequences. Here we provide evidence that the avian Hepatozoon-like parasites are most likely Lankesterella, supporting the suggestion that the systematic position of avian Hepatozoon-like species needs to be revised.

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