scholarly journals MOLECULAR DETECTION OF RANGELIA VITALII IN A DOG FROM THE CURITIBA METROPOLITAN REGION, PARANA STATE, SOUTHERN BRAZIL

2017 ◽  
Vol 22 (4) ◽  
Author(s):  
Anna C.B. Mongruel ◽  
Simone Tostes de Oileira Stedile ◽  
Marlos G Sousa ◽  
Jessica DM Valente ◽  
Iago C Barbosa ◽  
...  
Keyword(s):  
Weight Loss ◽  
18S Rdna ◽  
Molecular Detection ◽  
Clinical Care ◽  
Positive Sample ◽  
Metropolitan Region ◽  
Southern Brazil ◽  
Intermittent Fever ◽  
Mixed Breed ◽  
18S Rdna Sequence

Rangeliosis, caused by Rangelia vitalii, is known to causes apathy, intermittent fever, ear’s margin bleeding and anemia. Herein, we report the first molecular detection of R. vitalii infecting a dog from the Curitiba metropolitan region, Paraná State, southern Brazil. A four-year-old male mixed-breed neighborhood dog, presenting apathy, weight loss, and ear’s margin bleeding, was referred for clinical care at the Veterinary Teaching Hospital of the Universidade Federal do Paraná. Sequencing of the piroplasm positive sample showed ≥99% identity with R. vitalii 18S rDNA sequence deposited in GenBank. Further studies are needed to elucidate the pathogenicity, competence vector of ticks and epidemiology of this protozoa the State of Paraná.

Molecular Detection of Trypanosoma kaiowa in Tabanus triangulum (Diptera: Tabanidae) from the Coastal Plain of Rio Grande do Sul, Southern Brazil

2021 ◽  
Author(s):  
Gratchela D. Rodrigues ◽  
Eduardo Blodorn ◽  
Ândrio Zafalon-Silva ◽  
William Domingues ◽  
Roberta Marques ◽  
...  
Keyword(s):  
Coastal Plain ◽  
Molecular Detection ◽  
Rio Grande ◽  
Southern Brazil ◽  
Rio Grande Do Sul

Malaise, Weight Loss and Intermittent Fever

2019 ◽  
pp. 53-56
Author(s):  
Ross Petty ◽  
Farhad Salehzadeh ◽  
Marco Antonio Yamazaki-Nakashimada
Keyword(s):  
Weight Loss ◽  
Intermittent Fever

scholarly journals Eating disorders among health science students at a university in southern Brazil

2011 ◽  
Vol 33 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Luiza do Nascimento Ghizoni Pereira ◽  
Fabiana Schuelter Trevisol ◽  
João Quevedo ◽  
Luciano Kurtz Jornada
Keyword(s):  
Eating Disorders ◽  
Weight Loss ◽  
University Students ◽  
Eating Attitudes ◽  
Health Science ◽  
Science Students ◽  
Southern Brazil ◽  
Health Science Students ◽  
Weight Loss Strategies ◽  
Eat 26

OBJECTIVES: To analyze eating disorders among female university students and to assess the frequency of bulimia nervosa, anorexia nervosa, and inappropriate weight loss strategies in this population. METHODS: The sample comprised 214 female university students attending different health science programs at a university in southern Brazil, aged over 18 years, assessed using self-administered questionnaires. The 26-item version of the Eating Attitudes Test (EAT-26), the Bulimic Investigatory Test, Edinburgh (BITE), and a supplementary questionnaire covering data on weight status and inappropriate weight loss strategies were used to assess dietary abnormalities. RESULTS: Mean age (± standard deviation) was 21±9.93 years, and mean body mass index (BMI) was 21.1±2.59. Among the respondents, 72.9% said they would like to weigh less, 29% reported the use of different weight loss methods (diuretics were the most common, followed by laxatives, amphetamine-derived drugs, and self-induced vomiting). With regard to EAT-26 scores, 22.4% (95%CI 17.7-27.1) revealed abnormal feeding patterns; BITE indicated that 9.8% (95%CI 6.5-13.1) were at risk for developing bulimia and 36.9% (95%CI 31.5-42.3) required clinical evaluation. Mean BMI was lower among students with normal scores on both tests, but no association was found between BMI and satisfaction with own weight. CONCLUSION: There was a strong trend toward eating disorders in the health science students assessed, as demonstrated by EAT-26 and BITE scores; inadequate weight loss strategies are frequently used as well.

scholarly journals Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 505-513 ◽  
Author(s):  
Anna Cláudia Baumel Mongruel ◽  
Priscila Ikeda ◽  
Keyla Carstens Marques de Sousa ◽  
Jyan Lucas Benevenute ◽  
Margarete Kimie Falbo ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Phylogenetic Analyses ◽  
Southern Brazil ◽  
Conventional Pcr ◽  
Pathogenic Potential ◽  
Vector Borne ◽  
Pcr Assays ◽  
Etiological Agents ◽  
Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

Phylogenetic analyses of various euglenoid taxa (euglenozoa) based on 18s rdna sequence data

2000 ◽  
Vol 36 (1) ◽  
pp. 220-226 ◽  
Author(s):  
Angelika Preisfeld ◽  
Silke Berger ◽  
Ingo Busse ◽  
Susanne Liller ◽  
Hans Georg Ruppel
Keyword(s):  
18S Rdna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Rdna Sequence ◽  
Rdna Sequence Data ◽  
18S Rdna Sequence

Development of a PCR strategy for thraustochytrid identification based on 18S rDNA sequence

2002 ◽  
Vol 140 (5) ◽  
pp. 883-889 ◽  
Author(s):  
Caiqing Mo ◽  
Douek J. ◽  
Rinkevich B.
Keyword(s):  
18S Rdna ◽  
Rdna Sequence ◽  
18S Rdna Sequence

scholarly journals High occurrence of felid alphaherpesvirus 1 and feline calicivirus in domestic cats from southern Brazil

2020 ◽  
Vol 40 (9) ◽  
pp. 685-689
Author(s):  
Alice S. Becker ◽  
Francielle Liz Monteiro ◽  
Ana Carolina A. Scariot ◽  
Domitila B. Chagas ◽  
Geferson Fischer ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Clinical Signs ◽  
Feline Calicivirus ◽  
Ocular Injury ◽  
Frequency Of Occurrence ◽  
Southern Brazil ◽  
Domestic Cats ◽  
Oral Swab ◽  
Viral Isolation ◽  
High Occurrence

ABSTRACT: Felid alphaherpesvirus 1 (FeHV-1) and feline calicivirus (FCV) affect cats worldwide. The aim of this study was to evaluate the frequency of occurrence of FeHV-1 and FCV in cats with clinical signs of respiratory, oral and/or ocular disease. Samples were collected from cats cared for in veterinary ambulatory and clinics and submitted to molecular detection and viral isolation. Of the 49 cats evaluated, 45 (92%) were positive for at least one of the viruses; 82% (40/49) were positive for FeHV-1 and 41% (20/49) for FCV. Of these, 31% (15/49) were coinfection cases. For FeHV-1, 45% (18/40) of the cats tested were positive from the collection of eye swab, and the same percentage (9/20) was obtained for the FCV by the oral swab. FeHV-1 and/or FCV were isolated in 35% (17/49) of the samples. The main clinical sign observed was ocular secretion in 71% (35/49) of cats, characterized as mild serous, purulent or serosanguineous, and in some cases associated with ocular injury and marked chemosis. Our findings demonstrate the high occurrence of FeHV-1 and FCV in domestic cats in southern Brazil and indicate that measures should be implemented to improve the diagnostic, prevention and management against of these important diseases.

Weight Loss in Breast Cancer Patient Management

2002 ◽  
Vol 20 (4) ◽  
pp. 1128-1143 ◽  
Author(s):  
Rowan T. Chlebowski ◽  
Erin Aiello ◽  
Anne McTiernan
Keyword(s):  
Breast Cancer ◽  
Weight Loss ◽  
Clinical Outcome ◽  
Cancer Patients ◽  
Clinical Care ◽  
Current Status ◽  
Current Evidence ◽  
Dietary Therapy ◽  
Breast Cancer Patients ◽  
Breast Cancer Outcome

PURPOSE: To systematically review and summarize evidence relevant to obesity and breast cancer clinical outcome, potential hormonal mediating mechanisms, and the current status of weight loss interventions for chronic disease management. METHODS: A comprehensive, formal literature review was conducted to identify 5,687 citations with key information from 159 references summarized in text and tables. This process included a search for all breast cancer studies exploring associations among survival or recurrence and obesity at diagnosis or weight gain after diagnosis using prospective criteria. RESULTS: On the basis of observational studies, women with breast cancer who are overweight or gain weight after diagnosis are found to be at greater risk for breast cancer recurrence and death compared with lighter women. Obesity is also associated with hormonal profiles likely to stimulate breast cancer growth. Recently, use of weight loss algorithms proven successful in other clinical settings that incorporate dietary therapy, physical activity, and ongoing behavior therapy have been endorsed by the National Institutes of Health and other health agencies. CONCLUSION: Although definitive weight loss intervention trials in breast cancer patients remain to be conducted, the current evidence relating increased body weight to adverse breast cancer outcome and the documented favorable effects of weight loss on clinical outcome in other comorbid conditions support consideration of programs for weight loss in breast cancer patients. Recommendations for the clinical care of overweight or obese breast cancer patients are offered.

scholarly journals Natural hemoplasma infection of cats in Cuiaba, Mato Grosso, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 875 ◽  
Author(s):  
Herica Makino ◽  
Daphine Ariadne Jesus de Paula ◽  
Valéria Regia Franco Sousa ◽  
Adriane Jorge Mendonça ◽  
Valéria Dutra ◽  
...  
Keyword(s):  
Blood Count ◽  
Clinical Care ◽  
Mato Grosso ◽  
Blood Samples ◽  
Factors Associated ◽  
Mixed Breed ◽  
The City ◽  
Veterinary Hospital ◽  
Candidatus Mycoplasma Turicensis

The aim of this research was to investigate natural hemoplasma infection in cats treated at the Veterinary Hospital of the Federal University of Mato Grosso, and the factors associated with infection. Blood samples from 151 cats of different sexes, breeds, and ages were analyzed by PCR and blood count. The overall occurrence of hemoplasma was 25.8%. Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum (CMhm)’, and ‘Candidatus Mycoplasma turicensis’ (CMt) were observed in 15.2%, 14.6% and 2.6% of cats, respectively. In 6.6 % of cases, co-infection was observed. Male felines or mixed breed cats were associated with infection by CMhm (P = 0.02 and 0.04, respectively). The data obtained demonstrated an occurrence of 25.8% for hemoplasma infection in felines coming from clinical care in the city of Cuiabá, where males were at higher risk of acquiring the infection by these agents, in addition to a higher risk for CMhm in felines with no specific breed.

scholarly journals Rationale, design and study protocol of the randomised controlled trial: Diabetes Interventional Assessment of Slimming or Training tO Lessen Inconspicuous Cardiovascular Dysfunction (the DIASTOLIC study)

BMJ Open ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. e023207 ◽  
Author(s):  
Gaurav Singh Gulsin ◽  
Emer M Brady ◽  
Daniel J Swarbrick ◽  
Lavanya Athithan ◽  
Joseph Henson ◽  
...  
Keyword(s):  
Weight Loss ◽  
Body Composition ◽  
Exercise Capacity ◽  
Controlled Trial ◽  
Clinical Care ◽  
Cardiopulmonary Exercise Testing ◽  
Exercise Programme ◽  
Meal Replacement ◽  
Supervised Exercise ◽  
Study Results

IntroductionDespite their young age and relatively short duration of disease, younger adults with type 2 diabetes (T2D) already have diastolic dysfunction and may be at risk of incipient heart failure. Whether weight loss or exercise training improve cardiac dysfunction in people with T2D remains to be established.Methods and analysisProspective, randomised, open-label, blind endpoint trial. The primary aim of the study is to determine if diastolic function can be improved by either a meal replacement plan or a supervised exercise programme, compared with guideline-directed care. A total of 90 obese participants with T2D (aged 18–65 years), diabetes duration <12 years and not on insulin treatment will be randomised to either guideline-directed clinical care with lifestyle coaching, a low-energy meal replacement diet (average ≈810 kcal/day) or a supervised exercise programme for 12 weeks. Participants undergo glycometabolic profiling, cardiopulmonary exercise testing, echocardiography and MRI scanning to assesses cardiac structure and function and dual-energy X-ray absorptiometry scanning for body composition. Key secondary aims are to assess the effects of the interventions on glycaemic control and insulin resistance, exercise capacity, blood pressure, changes in body composition and association of favourable cardiac remodelling with improvements in weight loss, exercise capacity and glycometabolic control.Ethics and disseminationThe study has full ethical approval, and data collection was completed in August 2018. The study results will be submitted for publication within 6 months of completion.Trial registration numberNCT02590822; Pre-results.

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