Molecular detection of Salmonella spp. isolated from apparently healthy pigeon in Mymensingh, Bangladesh and their antibiotic resistance pattern

This study is to survey the prevalence and antibiotic resistance pattern of Salmonella spp. isolated from the farms at Dak Lak province, Viet Nam. 139 farms including 5 civet farms, 14 pig farms and 120 duck farms were sampled and analyzed. The results showed that many samples collected from 120 duck farms,14 pig farms and 5 civet farms were positive positive for Salmonella spp. Four serogroups of Salmonella species were demonstrated, Salmonella group B (25 strains), Salmonella group non-typable (13 strains), Salmonella group D (8 strains) and Salmonella group C (2 strains). There were 50 % of Salmonella strains resisting to at least one antibiotic, 75 % of the strains expressing multiple antibiotic resistance. In particular, 3 strains Salmonella secreted to ESBL (Extented-spectrum- beta lactamse).

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Evaluation of Class 1 and 2 Integrons and Antibiotic Resistance Pattern in Salmonella enterica Isolated from Diarrheal Food-Borne Outbreaks in Iran

Journal of Food Quality and Hazards Control ◽

10.18502/jfqhc.6.3.1384 ◽

2019 ◽

Author(s):

S.F. Sayadnouri ◽

M.M. Soltan Dallal ◽

S. Akbarzadeh ◽

R. Mazaheri Nezhad Fard

Keyword(s):

Antibiotic Resistance ◽

Salmonella Enterica ◽

Control Measures ◽

Resistance Pattern ◽

Salmonella Spp ◽

Chi Square ◽

Class 1 Integron ◽

Antibiotic Resistance Pattern ◽

Food Borne ◽

Class 1

Background: Salmonella spp. are major causes of food-borne disease and have been identified among many diarrheal outbreaks. The major aim of the current investigation was to evaluate the class 1 and 2 integrons and antibiotic resistance pattern in Salmonella enterica isolated from diarrheal food-borne outbreaks in Iran. Methods: This study was carried out on 115 diarrheal feces samples obtained from food-borne outbreak in 2016 in Iran. Antimicrobial resistance patterns of 27 isolated S. enterica seovars and presence of class 1 and class 2 integrons in the serovars were investigated using conventional and molecular methods. Results were statistically analyzed using SPSS software v. 21 and Chi-Square test. Results: Overall, 27 S. enterica were characterized as 14 S. Paratyphi C, 7 S. Enteritidis, 5 S. Paratyphi D, and 1 S. Paratyphi A serovars. Results of molecular assay showed that class 1 integron presented in all and class 2 integron in three strains. All isolates with class 2 integron genes were resistant to almost all the antimicrobials. Conclusion: Most studied Salmonella strains from diarrheal food-borne outbreak of Iran in 2016 were multiple resistant to the highlighted antimicrobials. Knowledge about risk factor involving the salmonellosis and their control measures could help the national authorities to prevent the outbreaks. Further comprehensive studies with larger sample sizes are necessary to acquire more data about risk factors of multiple resistant Salmonella outbreaks in the country.

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Exploring Antibiotic Resistance Pattern of Escherichia coli, Salmonella spp., and Staphylococcus spp. Isolated from Eggs in Rajshahi

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2021.3.4.328 ◽

2021 ◽

Vol 3 (4) ◽

pp. 25-30

Author(s):

Md Hakimul Haque ◽

Md. Mizanur Rahman ◽

Md. Lovelu Miah ◽

Soshe Ahmed ◽

Md. Rabiul Islam Sazib ◽

...

Keyword(s):

Antibiotic Resistance ◽

Bacterial Pathogen ◽

Diffusion Method ◽

Resistance Pattern ◽

Salmonella Spp ◽

Isolation And Identification ◽

Disk Diffusion Method ◽

E Coli ◽

Antibiotic Resistance Pattern ◽

Staphylococcus Spp

Chicken eggs are a major component of people’s diets, with an average yearly consumption of approximately 103 eggs per person in Bangladesh. Eggs act as an important carrier of food-borne pathogen worldwide. The study was conducted to identify the prevalence and antibiotic resistance pattern of E. coli, Salmonella spp., and Staphylococcus spp., in eggs isolated from farms and different markets of Rajshahi, Bangladesh. A total of 60 eggs were collected randomly between April to December 2019. The isolation and identification of bacterial pathogen was done in accordance with standard procedures. The bacterial isolates were subjected to antibiotic susceptibility testing against seven commonly used antibiotics using Kirby-Bauer disk diffusion method. An overall prevalence of E. coli, Salmonella spp., and Staphylococcus spp. were found to be 35.0%, 28.33%, and 23.33%, respectively. E. coli were found highly resistant to penicillin (100%), tetracycline (80.95%), ampicillin (100%), and erythromycin (85.71%) and were sensitive to amoxicillin (71.42%), ciprofloxacin (85.71%), and gentamicin (95.23%). Salmonella spp. was highly resistant to penicillin (100%), erythromycin (82.35%) and tetracycline (82.35%), and was sensitive to gentamicin (94.11%), amoxicillin (76.47%) and ciprofloxacin (70.58%). Staphylococcus spp. was resistant to penicillin (100%), erythromycin (78.57%), tetracycline (85.71%), amoxicillin (100%), and ampicillin (100%) but sensitive to ciprofloxacin (85.71%), and gentamicin (92.85%). The higher prevalence of multidrug-resistant (MDR) bacteria can easily enter the food chain, which poses a public health threat.

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Assessment of Gut Bacteria Profile and Antibiotic Resistance Pattern Among Psychotropic Drug Users as Compared With Apparently Healthy Controls: Comparative Cross-sectional Study

10.21203/rs.3.rs-96883/v1 ◽

2020 ◽

Author(s):

Solomon Ali ◽

Zufan Hailu Marame ◽

Mubarek Abera ◽

Mulatu Gashaw

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Psychotropic Medications ◽

Gut Bacteria ◽

Diffusion Method ◽

Resistance Pattern ◽

Healthy Controls ◽

Isolation And Identification ◽

Antibiotic Resistance Pattern ◽

Apparently Healthy

Abstract Introduction: In the 21st century, antibiotic resistance is becoming one of the major global public health threats. Several complex factors are associated with the emergence and spread of antibiotic resistance. Emerging evidences are indicating that drugs used for chronic illness conditions might have a contribution for antibiotic resistance either through drug-drug interactions or metabolism of the drugs by gut microbiota.Objective: The aim of this study was to compare the bacteria profile and resistance patterns of gut bacteria isolated from participants using psychotropic drugs and apparently healthy controls. Methods: Socio demographic data were collected from patients using psychotropic medications and apparently healthy persons. Clinical data was collected from patient records. Stool samples were collected from 107 patients using psychotropic medications and 107 apparently healthy controls. Gut bacteria flora were isolated as per recommended bacteria culture isolation and identification methods. Antibiotic susceptibility test was done using the disk diffusion method and Mast disks were used to identify extended-spectrum betalactamase (ESBL) and/or AmpC producing isolates. Result: A total of 245 bacterial isolates were isolated and identified. From these 124 (50.6%) bacteria were isolated from patients using Psychotropic medications. There was no bacteria profile difference between the two groups. Escherichia coli was the prevalent [100(80.6%) and 102 (84.3%)] bacteria isolated from patients using psychotropic medications and apparently healthy controls, respectively. Escherichia coli isolated from patients using psychotropic medications showed significantly higher resistance against tested amoxicillin-clavulanic acid, cephalosporin (2nd, 3rd, 4th generations), Meropenem, ciprofloxacin and Tetracycline. The odds of isolating ESBL producing Enterobacteriaceae [(OR=2.3, 95% C.I: (1.4-4.0)] and MDR [OR=5.4, 95% C.I:(1.5-29.8)] was higher on patients using psychotropic medications. Conclusion: The observed antibiotic resistance pattern of bacteria isolated from guts of patients using psychotropic medications was very high. The magnitude of antibiotic resistance is more pronounced among E.coli isolates.

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