scholarly journals Nelumbo nucifera leaf extract mediated synthesis of silver nanoparticles and their antimicrobial properties against some human pathogens

2015 ◽  
Vol 6 (3) ◽  
pp. 409-415 ◽  
Author(s):  
G. Premanand ◽  
N. Shanmugam ◽  
N. Kannadasan ◽  
K. Sathishkumar ◽  
G. Viruthagiri
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases


Author(s):  
Anuoluwa Abimbola Akinsiku ◽  
Kolawole Oluseyi Ajanaku ◽  
Joseph Adeyemi Adekoya ◽  
Olugbenga Samson Taiwo ◽  
Joan Ayo-Ajayi ◽  
...  

2019 ◽  
Vol 15 (4) ◽  
pp. 427-436
Author(s):  
Mahadevan Subramaniyan ◽  
Vijayakumar Subramaniyan ◽  
Arulmozhi Praveenkumar

Background: Infectious diseases are major leading cause of death in all parts of the world, because of the appearance of new multi drug resistant microbes. Therefore, the discovery of potential drug for effective treatment will help the slaughter of the microbes. The aim of the present study is to evaluate the presence of photochemical and antimicrobial activities of various crude extracts of leaves, fruits and root bark of Atalantia monophylla and Atalantia racemosa against human pathogens by using well diffusion method. Methods: Antimicrobial properties of the various extracts of Atalantia monophylla and Atalantia racemosa were studied against some human pathogenic microbes such as Gram-positive Bacteria, (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus) Gram-negative Bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli) and human opportunistic fungal pathogens (Candida albicans and Aspergillus niger). All the extracts were comparable with standard drugs (Ciprofloxacin, Gentamicin, Nystatin. and Amphotericin B). Minimum inhibitory concentration (MIC), minimum bactericidal /fungicidal concentration (MBC/MFC) values were determined through a microdilution method. The phytochemical analysis of these plant extracts were carried out using standard mthods. Results: Methanolic leaf extract of A. monophylla has showed excellent antimicrobial activity against S. aureus (40mm). As well, the A. racemosa methanolic leaf extract shows notable inhibitory activity against S. aureus (38mm). At the same time, the least inhibition was observed in aqueous extract of A. monophylla against E.coli (9mm). The MIC ranged from 0.78 µg/mL to 50 µg/mL and MBC/MFC 1.56 to 50 µg/mL were recorded. Phytochemical analysis of alkaloids, steroids, saponins, flavonoids, tannins, terpenoids, phenolics and cardiae glycoside were recorded in various extracts of A. monophylla and A. racemosa respectively. Flavonoids, phenolics and cardiac glycoside were present only in methonalic leaf extract of A. monophylla. Conclusion: The result of this study concluded that methanolic leaf extract has possessed novel compounds with significant antimicrobial properties. Hence, we recommend this plant for further studies on the isolation and characterization of that lead antimicrobial potential molecule.


2019 ◽  
Vol 13 (1) ◽  
pp. 1-6
Author(s):  
Didem Demir ◽  
Seda Ceylan ◽  
Gülşah Gül ◽  
Zeynep İyigündoğdu ◽  
Nimet Bölgen

In this study, Polyvinyl alcohol/Starch (PVA/Starch) cryogel scaffolds were combined with antibacterial silver nanoparticles (AgNPs), and the antimicrobial properties of composite scaffolds were determined for potential in tissue engineering applications. The porous PVA/Starch scaffolds were prepared by cryogelation technique. The nanoparticles were prepared by green synthesis from Aloe barbadensis leaf extract and characterized. The antibacterial, antifungal and antiyeast properties of AgNPs and AgNPs loaded PVA/Starch cryogel scaffolds were investigated. The highest antimicrobial activity of composite scaffold was found against Pseudomonas aeruginosa. Based on our studies, the results indicate that biodegradable, biocompatible and antimicrobial AgNPs loaded PVA/Starch scaffolds have potential to be used at an infection site in tissue engineering applications.


2018 ◽  
Vol 6 (10) ◽  
pp. 1397-1403
Author(s):  
Shantanu Raikar ◽  
◽  
ChetanJ D ◽  
MangeshS Jadhav ◽  
Sameer Kulkarni ◽  
...  

2010 ◽  
Vol 108 (3) ◽  
pp. 693-702 ◽  
Author(s):  
Thirunavukkarasu Santhoshkumar ◽  
Abdul Abdul Rahuman ◽  
Govindasamy Rajakumar ◽  
Sampath Marimuthu ◽  
Asokan Bagavan ◽  
...  

Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.


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