Evaluation of Setaria viridis responses to salt treatment and potassium supply: a characterization of three contrasting accessions

2021 ◽  
Author(s):  
Filipe Rodrigues Valeriano ◽  
Stéfanie Menezes de Moura ◽  
João Travassos-Lins ◽  
Marcio Alves-Ferreira ◽  
Ricardo Cardoso Vieira ◽  
...  
Keyword(s):  
Setaria Viridis ◽  
Salt Treatment ◽  
Potassium Supply

scholarly journals Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections

2017 ◽  
Vol 65 (8) ◽  
pp. 431-444 ◽  
Author(s):  
Maddalena Maria Bolognesi ◽  
Marco Manzoni ◽  
Carla Rossana Scalia ◽  
Stefano Zannella ◽  
Francesca Maria Bosisio ◽  
...  
Keyword(s):  
Analysis Software ◽  
In Situ Characterization ◽  
Salt Treatment ◽  
Tissue Sections ◽  
Single Section ◽  
Fluorescent Image ◽  
Spectral Separation ◽  
Secondary Antibodies

Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labeled primary antibodies, spectral separation of fluorochromes, bleaching of the fluorophores or chromogens, blocking of previous antibody layers, all in various combinations. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, and scarcity of specialized skills or facilities. We have validated a method based on common primary and secondary antibodies and diffusely available fluorescent image scanners. It entails rounds of four-color indirect immunofluorescence, image acquisition, and removal (stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulfide cleavage and a detergent or by a chaotropic salt treatment, this latter followed by antigen refolding. More than 30 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software. Multiplexing on routine tissue sections is a high throughput tool for in situ characterization of neoplastic, reactive, inflammatory, and normal cells.

scholarly journals Characterization of SlBAG Genes from Solanum lycopersicum and Its Function in Response to Dark-Induced Leaf Senescence

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 947
Author(s):  
Mingming He ◽  
Yu Wang ◽  
Mohammad Shah Jahan ◽  
Weikang Liu ◽  
Abdul Raziq ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Cellular Functions ◽  
Salt Treatment ◽  
Chlorophyll Fluorescence Parameters ◽  
Chlorophyll Contents ◽  
Evolutionarily Conserved ◽  
Abiotic Stimuli ◽  
Transgenic Tomatoes ◽  
Dark Stress

The Bcl-2-associated athanogene (BAG) family is a group of evolutionarily conserved cochaperones involved in diverse cellular functions. Here, ten putative SlBAG genes were identified in tomato. SlBAG2 and SlBAG5b have the same gene structure and conserved domains, along with highly similar identity to their homologs in Arabidopsis thaliana, Oryza sativa, and Triticum aestivum. The qPCR data showed that BAG2 and BAG5b were highly expressed in stems and flowers. Moreover, both genes were differentially expressed under diverse abiotic stimuli, including cold stress, heat stress, salt treatment, and UV irradiation, and treatments with phytohormones, namely, ABA, SA, MeJA, and ETH. Subcellular localization showed that SlBAG2 and SlBAG5b were located in the cell membrane and nucleus. To elucidate the functions in leaf senescence of BAG2 and BAG5b, the full-length CDSs of BAG2 and BAG5b were cloned, and transgenic tomatoes were developed. Compared with WT plants, those overexpressing BAG2 and BAG5b had significantly increased chlorophyll contents, chlorophyll fluorescence parameters and photosynthetic rates but obviously decreased ROS levels, chlorophyll degradation and leaf senescence related gene expression under dark stress. Conclusively, overexpression SlBAG2 and SlBAG5b could improve the tolerance of tomato leaves to dark stress and delay leaf senescence.

scholarly journals Molecular Characterization of cDNA Encoding Oxygen Evolving Enhancer Protein 1 Increased by Salt Treatment in the Mangrove Bruguiera gymnorrhiza

2000 ◽  
Vol 41 (11) ◽  
pp. 1279-1285 ◽  
Author(s):  
Koichi Sugihara ◽  
Nobutaka Hanagata ◽  
Zvy Dubinsky ◽  
Sigeyuki Baba ◽  
Isao Karube
Keyword(s):  
Molecular Characterization ◽  
Bruguiera Gymnorrhiza ◽  
Salt Treatment ◽  
Oxygen Evolving

scholarly journals Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections

2017 ◽  
Author(s):  
Maddalena Maria Bolognesi ◽  
Marco Manzoni ◽  
Carla Rossana Scalia ◽  
Stefano Zannella ◽  
Francesca Maria Bosisio ◽  
...  
Keyword(s):  
Analysis Software ◽  
In Situ Characterization ◽  
Salt Treatment ◽  
Tissue Sections ◽  
Single Section ◽  
Fluorescent Image ◽  
Spectral Separation ◽  
Secondary Antibodies

ABSTRACTMultiplexing (mplx), labeling for multiple immunostains the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labelled primary antibodies, spectral separation of fluorochromes, bleaching of the fluorophores or chromogens, blocking of previous antibody layers, all in various combinations. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities.We have validated a method based on common primary and secondary antibodies and diffusely available fluorescent image scanners. It entails rounds of four-color indirect immunofluorescence, image acquisition and removal (stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage and a detergent or by a chaotropic salt treatment, this latter followed by antigen refolding. More than thirty different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software. Mplx on routine tissue sections is a high throughput tool for in situ characterization of neoplastic, reactive, inflammatory and normal cells.

scholarly journals Transcriptomic Responses of Cordyceps militaris to Salt Treatment During Cordycepins Production

2021 ◽  
Vol 8 ◽  
Author(s):  
Gongbo Lv ◽  
Yue Zhu ◽  
Xiaojie Cheng ◽  
Yan Cao ◽  
Bin Zeng ◽  
...  
Keyword(s):  
Biological Activities ◽  
Expression Patterns ◽  
Bioactive Compound ◽  
Treated Group ◽  
Cordyceps Militaris ◽  
Control Group ◽  
Salt Treatment ◽  
Industrial Strains ◽  
Preliminary Study

Cordycepin is a major bioactive compound found in Cordyceps militaris (C. militaris) that exhibits a broad spectrum of biological activities. Hence, it is potentially a bioactive ingredient of pharmaceutical and cosmetic products. However, overexploitation and low productivity of natural C. militaris is a barrier to commercialization, which leads to insufficient supply to meet its existing market demands. In this study, a preliminary study of distinct concentrations of salt treatments toward C. militaris was conducted. Although the growth of C. militaris was inhibited by different salt treatments, the cordycepin production increased significantly accompanied by the increment of salt concentration. Among them, the content of cordycepin in the 7% salt-treated group was five-fold higher than that of the control group. Further transcriptome analysis of samples with four salt concentrations, coupled with Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, several differentially expressed genes (DEGs) were found. Finally, dynamic changes of the expression patterns of four genes involved in the cordycepin biosynthesis pathway were observed by the quantitative real-time PCR. Taken together, our study provides a global transcriptome characterization of the salt treatment adaptation process in C. militaris and facilitates the construction of industrial strains with a high cordycepin production and salt tolerance.

Genome-wide characterization of the laccase gene family in Setaria viridis reveals members potentially involved in lignification

Planta ◽  
2020 ◽  
Vol 251 (2) ◽  
Author(s):  
Marcella Siqueira Simões ◽  
Gabriel Garon Carvalho ◽  
Sávio Siqueira Ferreira ◽  
José Hernandes-Lopes ◽  
Nathalia de Setta ◽  
...  
Keyword(s):  
Gene Family ◽  
Setaria Viridis ◽  
Laccase Gene ◽  
Genome Wide

Potassium supply promotes the mitigation of NaCl-induced effects on leaf photochemistry, metabolism and morphology of Setaria viridis

2021 ◽  
Vol 160 ◽  
pp. 193-210
Author(s):  
Ana Carolina Mendes Bezerra ◽  
David da Cunha Valença ◽  
Nicia Eloísa da Gama Junqueira ◽  
Cristina Moll Hüther ◽  
Junior Borella ◽  
...  
Keyword(s):  
Setaria Viridis ◽  
Potassium Supply

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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