scholarly journals Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture

2020 ◽  
Author(s):  
Carla Simone Vizzotto ◽  
Julianna Peixoto ◽  
Stefan Joshua Green ◽  
Fabyano Alvares C. Lopes ◽  
Marcelo Henrique S. Ramada ◽  
...  
Keyword(s):  
Cyanobacterial Culture

First record of the soil cyanobacterium Nodosilinea epilithica (Synechococcales, Cyanobacteria) in Russia

2016 ◽  
Vol 50 ◽  
pp. 125-141
Author(s):  
A. D. Temraleeva ◽  
S. A. Dronova
Keyword(s):  
Chestnut Soil ◽  
First Record ◽  
Molecular Analyses ◽  
Dry Steppes ◽  
Cyanobacterial Culture ◽  
Volgograd Region ◽  
First Time

Nodosilinea epilithica Perkerson et Casamatta is reported for the first time for Russia. The sample was isolated from a typical chestnut soil in the zone of dry steppes (Volgograd Region) and its identity was confirmed by morphological and molecular analyses. The specific feature of the studied strain is its ability to form nodules at normal (60–75 μmol photons ∙ m-2 ∙ sec-1) light. The number of nodules is supposed to be related to the age of a cyanobacterial culture.

scholarly journals The Role of Selected Wavelengths of Light in the Activity of Photosystem II in Gloeobacter violaceus

2021 ◽  
Vol 22 (8) ◽  
pp. 4021
Author(s):  
Monika Kula-Maximenko ◽  
Kamil Jan Zieliński ◽  
Ireneusz Ślesak
Keyword(s):  
Photosystem Ii ◽  
Spectral Composition ◽  
Photosynthetic Electron Transport ◽  
Metabolic Energy ◽  
Light Conditions ◽  
Gloeobacter Violaceus ◽  
Transport Chain ◽  
Cyanobacterial Culture ◽  
Photosynthetic Antennas

Gloeobacter violaceus is a cyanobacteria species with a lack of thylakoids, while photosynthetic antennas, i.e., phycobilisomes (PBSs), photosystem II (PSII), and I (PSI), are located in the cytoplasmic membrane. We verified the hypothesis that blue–red (BR) light supplemented with a far-red (FR), ultraviolet A (UVA), and green (G) light can affect the photosynthetic electron transport chain in PSII and explain the differences in the growth of the G. violaceus culture. The cyanobacteria were cultured under different light conditions. The largest increase in G. violaceus biomass was observed only under BR + FR and BR + G light. Moreover, the shape of the G. violaceus cells was modified by the spectrum with the addition of G light. Furthermore, it was found that both the spectral composition of light and age of the cyanobacterial culture affect the different content of phycobiliproteins in the photosynthetic antennas (PBS). Most likely, in cells grown under light conditions with the addition of FR and G light, the average antenna size increased due to the inactivation of some reaction centers in PSII. Moreover, the role of PSI and gloeorhodopsin as supplementary sources of metabolic energy in the G. violaceus growth is discussed.

Detection of microcystin-producingMicrocystisin Guanqiao Lake using a sandwich hybridization assay

2012 ◽  
Vol 58 (4) ◽  
pp. 442-447 ◽  
Author(s):  
Jing Ping Zhu ◽  
Shi Du ◽  
Xian Li
Keyword(s):  
Calibration Curve ◽  
Cell Number ◽  
Hybridization Assay ◽  
Sequence Analyses ◽  
Sandwich Hybridization ◽  
Cyanobacterial Culture ◽  
Field Samples ◽  
Laboratory Cultures ◽  
Pcr Technology ◽  
Sandwich Hybridization Assay

Based on sequence analyses of the mcyJ gene from Microcystis strains, a probe pair TJF and TJR was designed and a sandwich hybridization assay (SHA) was established to quantitatively detect microcystin-producing Microcystis. Through BLAST and cyanobacterial culture tests, TJF and TJR were demonstrated to be specific for microcystin-producing Microcystis. A calibration curve for the SHA was established, and the lowest detected concentration was 100 cells·mL–1. Laboratory cultures and field samples from Guanqiao Lake were analyzed with both the SHA and microscopy. The cell number of microcystin-producing Microcystis and that of total Microcystis were compared. The biotic and abiotic components of the samples were of little disturbance to the SHA. In this study, a SHA was established to detect Microcystis, providing an alternative to PCR–ELISA and real-time PCR technology.

scholarly journals Cyanobacterial Culture Acts as Good Organic Biostimulant on Anethum graveolens

2018 ◽  
Vol 7 (12) ◽  
pp. 787-793
Author(s):  
Jayant Pralhad Rathod ◽  
Swapnil I. Lambsonge ◽  
Darasing R. Rathod ◽  
Rajendra M. Gade
Keyword(s):  
Anethum Graveolens ◽  
Cyanobacterial Culture

scholarly journals Prospects in Cadmium-Contaminated Water Management Using Free-Living Cyanobacteria (Oscillatoria sp.)

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 542
Author(s):  
Iván Carralero Bon ◽  
Lucas M. Salvatierra ◽  
Luciana D. Lario ◽  
Jordi Morató ◽  
Leonardo M. Pérez
Keyword(s):  
Metal Removal ◽  
Soluble Carbohydrates ◽  
Free Living ◽  
Carotenoid Concentration ◽  
Cyanobacterial Culture ◽  
Cellular Energetics ◽  
Atr Spectroscopy ◽  
Chlorophyll A Content ◽  
Cd Exposure ◽  
Cd Removal

In this study, the removal of cadmium (Cd) by free-living Oscillatoria sp. was studied. Our results showed that maximal Cd removal efficiency (~60%) by the cyanobacterial culture was achieved within 12–24 h in the presence of 5.0 or 25.0 mg/L of Cd. The mechanisms underlying this phenomenon were explored by elemental analysis and FTIR-ATR spectroscopy. It was found that metal adsorption by negatively charged functional groups in the cyanobacterial biomass was the main mechanism used by Oscillatoria sp. to remove metal from the aqueous medium, followed by Cd bioaccumulation into living cells. Additionally, Cd-exposed microalgae showed increased oxidative stress (MDA formation), a decreased dehydrogenase activity, a higher amount of soluble carbohydrates and a decreased total carotenoid concentration, as compared to the control cells. These results suggest that Oscillatoria sp. improved its antioxidative defense system under stressful conditions, through carotenoid-mediated ROS quenching and induction of carbohydrate catabolism, in order to counteract the oxidative damage and preserve the photosynthetic machinery and cellular energetics. In fact, no significant reduction in Oscillatoria sp. cell density, total protein amount, and chlorophyll a content was observed after 24-h Cd exposure, even at the highest metal concentration tested (i.e., 25.0 mg/L). Hence, the presented results are the first to describe some new insights about the metabolic and physiological behavior of living Oscillatoria sp. during Cd remediation, and open up the possibility of finding an equilibrium that maximizes metal removal performance with an active cyanobacterial metabolism, to achieve a rewarding and sustainable management of industrial metal-polluted wastewater.

scholarly journals Production of polyhydroxybutyrates and carbohydrates in a mixed cyanobacterial culture: Effect of nutrients limitation and photoperiods

2018 ◽  
Vol 42 ◽  
pp. 1-11 ◽  
Author(s):  
Dulce María Arias ◽  
Enrica Uggetti ◽  
María Jesús García-Galán ◽  
Joan García
Keyword(s):  
Cyanobacterial Culture

scholarly journals Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems

2021 ◽  
Author(s):  
I. V. N. Rathnayake ◽  
Thilini Munagamage ◽  
A. Pathirathne ◽  
Mallavarapu Megharaj
Keyword(s):  
Exposure Time ◽  
Storage Temperature ◽  
Sol Gel ◽  
Aquatic Systems ◽  
Operational Conditions ◽  
C Storage ◽  
Cyanobacterial Culture ◽  
Array Biosensor ◽  
Biosensor Array ◽  
Decreasing Ph

Abstract Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems.

scholarly journals Use of a Thermophile Desiccation-Tolerant Cyanobacterial Culture and Os Redox Polymer for the Preparation of Photocurrent Producing Anodes

2020 ◽  
Vol 8 ◽  
Author(s):  
Manuel Gacitua ◽  
Catalina Urrejola ◽  
Javiera Carrasco ◽  
Rafael Vicuña ◽  
Benjamín M. Srain ◽  
...  
Keyword(s):  
Redox Polymer ◽  
Cyanobacterial Culture

scholarly journals Draft Genome Sequence of Muricauda sp. Strain K001 Isolated from a Marine Cyanobacterial Culture

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Carla S. Vizzotto ◽  
Fabyano A. C. Lopes ◽  
Stefan J. Green ◽  
Andrei S. Steindorff ◽  
Juline M. Walter ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Sequence ◽  
Heterotrophic Bacteria ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Cyanobacterial Culture

ABSTRACT We report the whole-genome sequence of Muricauda sp. strain K001 isolated from a marine cyanobacterial culture. This genome sequence will improve our understanding of the influence of heterotrophic bacteria on the physiology of cyanobacteria and may contribute to the development of new natural products.

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