scholarly journals Cost-reduction strategies in massive genomics experiments

2019 ◽  
Vol 1 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Haichao Li ◽  
Kun Wu ◽  
Chenchen Ruan ◽  
Jiao Pan ◽  
Yujin Wang ◽  
...  

Abstract Many modern biology studies require deep, whole-genome sequencing of hundreds to thousands of samples. Although per-sample costs have dramatically decreased, the total budget for such massive genome sequencing constitutes a significant barrier for poorly funded labs. The costly lab tools required for genomics experiments further hinder such studies. Here, we share two strategies for extensively reducing the costs of massive genomics experiments, including miniaturization of the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (reducing the per-sample total costs to ~ 1/6 of that charged by service providers) and in-lab 3D model-designing of genomics tools. These strategies not only dramatically release funding pressure for labs, but also provide students with additional training in hands-on genomics and 3D-model-designing skills, demonstrating the high potential for their application in genomics experiments and science education.

1998 ◽  
Vol 79 (2) ◽  
pp. 160-172 ◽  
Author(s):  
Elizabeth W. Lindsey

Homeless shelter directors in two southern states were surveyed to ascertain their perceptions of factors that help or hinder homeless, mother-headed families in emerging from homelessness. Respondents believed that mothers' attitudes and motivation were the most important factors in getting and keeping housing and that lack of social supports and relationship difficulties were the most significant problems families faced in their attempts to emerge from homelessness. Scarce housing was seen as the most significant barrier within the community. The findings are compared with findings from other studies, and explanations for differing results are presented. The author discusses the implications of the findings for service providers and communities in light of recent welfare-reform legislation.


Author(s):  
Ganesh Chandra Deka ◽  
Prashanta Kumar Das ◽  
Rahul Borah

Virtual Routing and Forwarding (VRF) is a technology that allows multiple instances of IP (Internet Protocol) routing table to co-exist within the same Router at the same time. The routing instances are independent, allowing the same or overlapping IP addresses to be used without conflict. Using VRF technology, users can virtualize a network device from a Layer 3 standpoint of creating different “Virtual Routers” in the same physical device. Internet Service Providers (ISP) often use VRF technology to create separate routing table in a single physical Router which are completely isolated one from the others. This chapter discusses about the configuration of VRF-Lite in GNS3 (Graphical Network Simulator-3) on RIP/v2, EIGRP and OSPF protocols.


2019 ◽  
Author(s):  
Doreen Kalina

In order for us to overcome the much maligned segregation of able-bodied people and people with disabilities in the job market and in vocational training, training that both caters to the needs of people with disabilities and is either on-the-job or hands-on is indispensable. If companies worked with disabled young people early on, they would be able to break down barriers and deconstruct stereotypes more easily. In order to achieve inclusivity in vocational training, separation has to be avoided as much as possible. Using this humanitarian premise as a starting point, this study examines a tiered system between in-company and extra-company training and, at the same time, between training in an officially recognised profession and skilled worker apprenticeships. In doing so, it focuses on the model of integrated training laid down in § 51, par. 2 of the Sozialgesetzbuch IX (Germany’s social code), which is advancing the current debate on the transformation from institutional to individual rehabilitation by placing demands on service providers.


2015 ◽  
Author(s):  
Madison I Dunitz ◽  
Jenna M Lang ◽  
Guillaume Jospin ◽  
Aaron E Darling ◽  
Jonathan A Eisen ◽  
...  

The sequencing, assembly, and basic analysis of microbial genomes, once a painstaking and expensive undertaking, has become almost trivial for research labs with access to standard molecular biology and computational tools. However, there are a wide variety of options available for DNA library preparation and sequencing, and inexperience with bioinformatics can pose a significant barrier to entry for many who may be interested in microbial genomics. The objective of the present study was to design, test, troubleshoot, and publish a simple, comprehensive workflow from the collection of an environmental sample (a swab) to a published microbial genome; empowering even a lab or classroom with limited resources and bioinformatics experience to perform it.


2021 ◽  
Author(s):  
Michael Ramon ◽  
Tony Wooley ◽  
Kyle Martens ◽  
Amy Farrar ◽  
Seth Fadaol

Abstract The culture of safety within the oil and gas industry has undergone an evolution since the advent of significant E&P operations in the late 1800s. The initial focus on safety was to protect property, not people. This mentality has shifted over time to include a greater focus on the safety of personnel, in parallel with technology developments that have pushed the limits of operators’ and service providers’ abilities to drill and complete more complicated wells. The safety efforts introduced to date have yielded results in every major HS&E category; however, falls and dropped objects continue to be areas in need of improvement. During cementing rig up and operations there are still many manual activities that require working at heights in the derrick. New technological advances have allowed the industry to reduce the number of hands-on activities on the rig and operators have moved to eliminate these activities by automating operations. Man lifting operations are recognized as a high-risk activity and, as such, many rigs require special permitting. During cementing operations, not only are personnel lifted into hazardous positions, but they are usually equipped with potential dropped objects. Some of these objects, if dropped, reach an impact force that could seriously injure or, in worst cases, result in a fatality. During these operations, personnel are also hoisted along with a heavy cement line in very close proximity. This introduces other dangers such as tangling, pinch points, and blunt force trauma. These risks are heavily increased when working in adverse conditions, such as high winds or rough seas. By utilizing a wireless cement line make up device, along with wireless features on a cement head to release the darts/plugs/balls and operate the isolation valves, an operator can eliminate the need for hands-on intervention. This paper will discuss current cement head technologies available to the operator that allow them to improve safety and efficiencies in operational rig time. Three field studies will be presented that detail running cement jobs with all functions related to the wireless attributes of the cement head. The field studies will present the operational efficiencies achieved by utilizing the wireless features compared to the standard manual method. Before the recent introduction of a wireless cementing line make-up device, a wireless cement head still required hands-on intervention to rig up the tools, putting people in high-risk situations.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Laura Fuentes-Moraleda ◽  
Ana Muñoz-Mazón ◽  
Coral Santiago-Rincón ◽  
Alicia Orea-Giner

PurposeThis exploratory study aims to identify the main risk reduction strategies when individuals suffer from coeliac disease (CD) or non-coeliac gluten sensitivity (NCGS) travel. Based on Yeung and Yee's (2013; 2019) model, the paper offers a new framework for analysing the main travel risk reduction strategies for people with specific food needs. The empirical work focuses on the perspectives of different stakeholders (CD and NCGS community, restaurant managers/service providers and nutritionists).Design/methodology/approachA qualitative approach based on thematic analysis is adopted. The results of 32 semi-structured in-depth interviews reveal different stakeholders' perspectives in order to understand the various strategies.FindingsThe findings confirm the high level of consensus that the main food risk reducers are: travel information, staff training in safety assurance, legislation and risk prevention protocols. The findings also show significant limitations in the information offered by restaurants, organisations and tourist destinations and the negative repercussions on the tourist experience and the reputation on a particular destination.Practical implicationsThe results will help hospitality business managers and destination management organisations develop food risk reduction strategies to solve some of the most important food-related problems when people in this market segment travel.Originality/valueThis work contributes to the literature by providing a new framework on travel risk reduction strategies for people with specific food needs. The novelty of this research is mainly found in the study of risk reduction strategies related to the travel decision-making process for those with CD and NCGS from different perspectives.


2002 ◽  
Vol 55 (10) ◽  
pp. 1163-1188 ◽  
Author(s):  
P. Monique Valcour

This article uses ethnographic data gathered from a parent cooperative nursery school to examine the strategies managers use to resolve role conflict in a multiplex role system, that is, a setting in which the relationship between managers and the people they supervise is based on more than one set of roles. In the organization under study, teachers functioned as both managers of, and service providers to, the parent volunteers they were charged with supervising. Parents' volunteer worker role was subject to conflict with and interruption by the expectations of their organizational client role, which constrained teachers' ability to direct parents' work in the classroom. Managerial effectiveness required responding to the demands and expectations of both sets of roles through a process of adaptive self-regulation. Teachers used several discrepancy detection strategies to gather information on parent volunteers' relative role salience, role transition readiness, and volunteer skills. Based on the information obtained, they devised discrepancy reduction strategies to focus parents' attention on the desired role for task performance and to reduce discrepancies between parents' expectations for managerial behavior and their own actions. The most effective teachers were able to develop personal relationships with parent volunteers, elicit knowledge about parents' role expectations and capabilities, and respond to the expectations of parents' multiple roles on an individualized basis.


2019 ◽  
Vol 2 (4) ◽  
pp. 732-760 ◽  
Author(s):  
Jenny R Isaacs

This article applies Mary Louise Pratt’s “contact” perspective within a multispecies ethnography of conservation encounters on the Delaware Bay. Using critical insights from decolonial feminist science studies, environmental geography, and critical animal studies, the article deconstructs technoscientific environmental knowledge production within a more-than-human contact zone. The tools, technologies, and “conspicuous innocence” of hands-on shorebird conservation research practices are described. Re-inscribing nonhuman agency and colonial histories of place, it argues that certain elements of conservation research may be fairly read as “violent” expressions of “animality/coloniality” and “anti-conquest.” It concludes by offering some harm reduction strategies for improving conservation and critical environment studies.


2020 ◽  
Vol 02 (03) ◽  
pp. 2020002
Author(s):  
Julia Woithe ◽  
Alexandra Jansky ◽  
Oliver Keller ◽  
Tiago Gonçalves

The ATLAS detector is the largest particle detector at the LHC and one of the most complex machines ever built. It allows precise measurements of particles emanating from proton collisions. Due to its complexity, introducing the ATLAS detector in the high-school physics classroom can be challenging. Nonetheless, we show how to use 3D printing to provide a hands-on classroom activity by constructing a functional 3D model of the toroidal ATLAS magnet system. This model can be used to discover, visualize and explain the shape of a toroidal magnetic field and to start a discussion about the role of magnetic fields in particle detectors in general.


2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nguyen Toan Tran ◽  
Alison Greer ◽  
Talemoh Dah ◽  
Bibiche Malilo ◽  
Bergson Kakule ◽  
...  

Abstract Background Fragile and crisis-affected countries account for most maternal deaths worldwide, with unsafe abortion being one of its leading causes. This case study aims to describe the Clinical Outreach Refresher Training strategy for sexual and reproductive health (S-CORT) designed to update health providers’ competencies on uterine evacuation using both medications and manual vacuum aspiration. The paper also explores stakeholders’ experiences, recommendations for improvement, and lessons learned. Methods Using mixed methods, we evaluated three training workshops that piloted the uterine evacuation module in 2019 in humanitarian contexts of Uganda, Nigeria, and the Democratic Republic of Congo. Results Results from the workshops converged to suggest that the module contributed to increasing participants’ theoretical knowledge and possibly technical and counseling skills. Equally noteworthy were their confidence building and positive attitudinal changes promoting a rights-based, fearless, non-judgmental, and non-discriminatory approach toward clients. Participants valued the hands-on, humanistic, and competency-based training methodology, although most regretted the short training duration and lack of practice on real clients. Recommendations to improve the capacity development continuum of uterine evacuation included recruiting the appropriate health cadres for the training; sharing printed pre-reading materials to all participants; sustaining the availability of medication and supplies to offer services to clients after the training; and helping staff through supportive supervision visits to accelerate skills transfer from training to clinic settings. Conclusions When the lack of skilled human resources is a barrier to lifesaving uterine evacuation services in humanitarian settings, the S-CORT strategy could offer a rapid hands-on refresher training opportunity for service providers needing an update in knowledge and skills. Such a capacity-building approach could be useful in humanitarian and fragile settings as well as in development settings with limited resources as part of an overall effort to strengthen other building blocks of the health system.


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