Scientific division, committee on enzymes IFCC methods for the measurement of catalytic concentration of enzymes Part 7. IFCC method for creatine kinase22This paper forms part of a series of recommendations on measurements of catalytic concentrations of enzymes. Others deal with: Part 1. General conditions, [1](Approved 1979). Part 2. Method for aspartate aminotransferase, [2](Approved 1985). Part 3. Method for alanine aminotransferase, [3](Approved 1985). Part 4. Method for γ-glutamyltransferase, [4]. Part 5. Method for alkaline phosphatase, [5]. Part 6. Reference materials for enzyme measurements (Stage 1, draft 1989 — copy available from Committee Chairman). (ATP: Creatine N-phosphotransferase, EC 2.7.3.2). IFCC recommendation

1990 ◽  
Vol 190 (1-2) ◽  
pp. S4-S17 ◽  
Keyword(s):  
Alkaline Phosphatase ◽  
Reference Materials ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Committee Chairman ◽  
Stage 1 ◽  
General Conditions

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Intraperitoneal Administration ◽  
Chloroform Extract ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
High Dose ◽  
Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

scholarly journals Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples

2017 ◽  
Vol 62 (No. 6) ◽  
pp. 342-350
Author(s):  
CS Lin ◽  
GH Chiang ◽  
CH Liu ◽  
HC Tsai ◽  
CC Yang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bile Acid ◽  
Blood Urea Nitrogen ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Total Bile Acid ◽  
Analytical Performance ◽  
Blood Samples

In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShield<sup>TM</sup>, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShield<sup>TM</sup> and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument’s analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser’s performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (&lt; 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application.

scholarly journals Biochemical Effects to Toxicity of CCl4 on Rosy Barbs (Puntius conchonius)

Our Nature ◽  
1970 ◽  
Vol 3 (1) ◽  
pp. 20-25 ◽  
Author(s):  
H. Bhattacharya ◽  
L. Lun ◽  
G.D. Gomez R.
Keyword(s):  
Alkaline Phosphatase ◽  
Lactate Dehydrogenase ◽  
Enzymatic Activity ◽  
Blood Urea Nitrogen ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Target Organ ◽  
Biochemical Changes ◽  
Urea Nitrogen ◽  
Biochemical Effects

Biochemical changes in the liver, kidneys and gills of rosy barbs due to toxicity of CCl4 were measured after 96 hour exposure. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and creatinin (CRN), levels were measured. Significant increase in ALP, ALT, LDH and BUN activities were observed in the liver in the treated groups compared to controls (P < 0.05). AST level was significantly higher in the kidneys. This study indicates that the enzymatic activity was comparatively higher in the liver than kidneys or gills, suggesting that the liver is the target organ of CCL4 toxicity to rosy barbs.Keywords: Toxicity, Rosy Barb, CCl4doi:10.3126/on.v3i1.330Our Nature (2005)5:20-25

scholarly journals A Comparative Study on the Hepatoprotective Effect of Tamarindus indica and Vitamin E in Long Evans Rats

2014 ◽  
Vol 6 (2) ◽  
pp. 63-67 ◽  
Author(s):  
NJ Shammi ◽  
ZK Choudhry ◽  
MI Khan ◽  
MM Hossain
Keyword(s):  
Alkaline Phosphatase ◽  
Vitamin E ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Histopathological Examination ◽  
Protective Effects ◽  
Tamarindus Indica ◽  
Ethanolic Extracts ◽  
Long Evans

The protective effects of ethanolic extract of Tamarindus indica leaves and seeds in compoarison to vitamin E, were studied on paracetamol induced hepatotoxicity in Long Evans Rats. Different groups of animals were administered in the paracetamol (1500mg /kg, p.o.) for 7 days. Ethanolic extracts of leaves and seeds of Tamarindus indica (1250mg/kg) with parallel vitamin E (500 mg/kg), were administered to paracetamol pretreated rats. On treatment with paracetamol a significant increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin and alkaline phosphatase were observed. On administration of ethanolic extracts of leaves and seeds a significant decrease in the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin and alkaline phosphatase (ALP) were observed and histopathological examination of liver tissue revealed an almost return to normal architecture. The result were almost comparable to vitamin E, a known hepatoprotective agent. DOI: http://dx.doi.org/10.3329/bjmb.v6i2.17645 Bangladesh J Med Biochem 2013; 6(2): 63-67

Hepatotoxicity Associated with Cisplatin Chemotherapy

1993 ◽  
Vol 27 (4) ◽  
pp. 438-441 ◽  
Author(s):  
Robert J. Cersosimo
Keyword(s):  
Squamous Cell Carcinoma ◽  
Alkaline Phosphatase ◽  
Lactate Dehydrogenase ◽  
Cell Carcinoma ◽  
Liver Damage ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Case Reports ◽  
Normal Limits ◽  
Case Summary

OBJECTIVE: To report a case of possible cisplatin-associated hepatotoxicity. CASE SUMMARY: A 69-year-old man received three cycles of cisplatin (100 mg/m2) and fluorouracil (1000 mg/m2/d for five days) for management of squamous cell carcinoma of the head and neck. Liver enzyme concentrations were within normal limits prior to each cycle of therapy but the aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase concentrations increased on the second day of each cycle. The concentrations began to decline on day 3 of each course, despite continued fluorouracil administration, and returned to normal by day 10. The patient's antiemetic therapy included metoclopramide in cycle 1 and ondansetron in cycles 2 and 3, which may have contributed to the enzyme elevations. DISCUSSION: Case reports of cisplatin-associated hepatotoxicity are reviewed. An association between cisplatin administration and hepatotoxicity is proposed in this patient. CONCLUSIONS: This patient may have experienced cisplatin-induced liver damage. Metoclopramide and ondansetron may have contributed to this effect.

scholarly journals Epizootology and biochemical Pparameters of Dogs with babesiosis in barnaul

2020 ◽  
Vol 14 (2) ◽  
pp. 46-52
Author(s):  
N. M. Ponamarev ◽  
M. Yu. Novikova M. ◽  
N. V. Tikhaya
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Blood Cells ◽  
Alanine Aminotransferase ◽  
White Blood Cells ◽  
German Shepherd ◽  
Age Related ◽  
Calcium And Phosphorus ◽  
Conjugated Bilirubin

The purpose of the research is studying the epizootology of babesiosis and biochemical parameters of dogs with babesiosis in Barnaul. Materials and methods. The studies were conducted at the Department of Microbiology, Epizootology, Parasitology and Veterinary-Sanitary Examination of the Altai State Agrarian University and at the Barnaul Central Veterinary Hospital. Statistical analysis was performed according to the laboratory research journal from 2013 to 2017. Babesiosis was diagnosed based on clinical evidence and microscopic examination of peripheral blood smears. Alanine aminotransferase, aspartate aminotransferase, total and conjugated bilirubin, creatinine, urea, alkaline phosphatase, total protein, calcium and phosphorus contained in the blood serum was determined. In total, 1258 blood samples of dogs of different sex, age and breed were examined. In order to identify age-related features in hematological parameters, we formed 4 groups of 30 dogs in each age group. We studied indicators such as the erythrocyte sedimentation rate, the total number of red blood cells, and white blood cells, hemoglobin concentration and color index, and counted formed elements. The spread of ixodic ticks was studied in Barnaul and a suburban area of Chernitsky Forest. Ixodic ticks were collected on flag. A total of 535 live ticks of the Ixodidae family were collected. The species of ixodic ticks was identified using the Guide to arachnoentomoses. Results and discussion. The rate of babesia infection in dogs was 36.3%. Most often babesiosis occurs in outbred dogs, the terrier, German Shepherd dog, Cocker Spaniel, Central Asian Shepherd dog and dachshund. Males, especially those over the age of 8, are more infected with Babesia. Most cases of dog lesions by babesiosis occur in Zheleznodorozhny, Central and Industrial Districts of Barnaul. The disease is diagnosed from early April to late June, and from early August to late October. Significant indicators for identifying the pathology of individual systems and organs of the animal affected by babesiosis are aspartate aminotransferase, alanine aminotransferase, creatinine, urea, total and conjugated bilirubin, and alkaline phosphatase. The level of total protein under the age of two years is lowered, while calcium and phosphorus are within physiological values. 

scholarly journals The safety of prolong usage of diazepam on liver and renal functions in rats

2021 ◽  
Vol 2 (1) ◽  
pp. 059-061
Author(s):  
Wajdy Jabbar Majid ◽  
Tayseer Ali Talab
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Intraperitoneal Administration ◽  
Blood Chemistry ◽  
Serum Glucose ◽  
Male Rats ◽  
Renal Functions

In this study the effect of diazepam on some blood chemistry values was studied in male rats. Intraperitoneal administration of diazepam at a dose of 0.6mg/kg body weight for 60 days didn't induce significant changes in serum glucose, urea, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, creatinine and alkaline phosphatase levels. Findings of this study give further sound for the safety of benzodiazepines.

scholarly journals Aktivitas Beberapa Enzim Serum pada Kambing Ettawa

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Irkham Widiyono ◽  
Sarmin S ◽  
Bambang Suwignyo
Keyword(s):  
Alkaline Phosphatase ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Creatinine Kinase

Penelitian ini bertujuan mengkaji aktivitas enzim alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), dan creatinine kinase (CK) pada kambing peranakan Ettawa. Sebanyak 43 ekor kambing peranakan Ettawa dari berbagai kelompok status fisiologis (jantan muda, betina muda, jantan dewasa, betina dewasa, betina bunting, dan betina laktasi) yang secara klinis sehat dan tidak cacat digunakan dalam penelitian ini. Pemeriksaan klinis dan pengambilan sampel darah dilakukan sesuai dengan skedul pemeriksaan dan pengambilan sampel sesuai dengan standard klinik yang diuraikan Baumgartner (1999). Pengambilan sampel darah dilakukan pada jam 7.00-8.00 wib. Pemeriksaan enzim di dalam serum diperiksa dengan menggunakan metode standar sebagaimana diuraikan oleh Kraft dan Duer (1999). Aktivitas AST, ALT, ALP di dalam serum memiliki level yang sebanding dengan level pada ruminansia lain. Sementara itu, aktivitas CK lebih tinggi dibanding nilai yang dinyatakan di dalam literatur untuk ruminansia kecil lainnya.

scholarly journals Evalution of Anti-Diabetic Potential of Aqueous Extract of “Luffa cylindrica” (Native Sponge/Sponge Gourd) Leaf and Seed on Alloxan Induced Diabetic Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
Luka, C. D. Abigail ◽  
E. A. Metuaghan
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Normal Control ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Serum Alkaline Phosphatase ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Luffa Cylindrica ◽  
Leaf Extracts

The study was carried out to evaluate the anti-diabetic effect of Luffa cylindrical (native sponge /sponge gourd) seed and leaf extracts in alloxan- induced diabetic rats. Sixteen experimental rats were divided into four groups of four rats each: a, diabetic control; b, normal control; c, diabetic rats treated with seed extract (400 mg/kg) and d, diabetic rats treated with leaf extract (400 mg/kg). The groups A, C and D rats were induced with diabetes intraperitoneally with alloxan (150 mg/kg bw). Phytochemical screening was carried out on the plant seed and leaf extracts and the following biochemical tests were carried out: blood glucose, serum lipid profile, serum alanine aminotransferase, serum aspartate aminotransferase, serum alkaline phosphatase, total protein, albumin, creatinine, urea, uric acid and some electrolytes like Na+, K+, HCO3-, and Cl- the administration of alloxan to experimental rats resulted in an increased level of most biochemical parameters; blood glucose, serum alanine aminotransferase, serum aspartate aminotransferase and serum alkaline phosphatase, serum total cholesterol, triglyceride, low density lipoprotein, creatinine, urea and uric acid. Luffa cylindrica seed and leaf extracts was administered to groups c and d diabetic rats respectively for two weeks, results were compared with normal control and diabetic control rats these parameters were found to be significantly (p<0.05) high in the diabetic groups than in the normal control groups. Treatment with the plant extract significantly (p<0.05) reduced elevated blood levels of glucose, cholesterol, triglyceride, alkaline phosphatase, amylase, aspartate aminotransferase, alanine aminotransferase, creatinine, urea, uric acid associated with alloxan-induced diabetic rats. The plant tested positive for alkaloids, flavonoids, saponins and tannins, negative for cardiac glycosides, phenols, resins, terpenes and steroids. Extracts of Luffa cylindrica seed and leaf has shown to have anti-diabetic and anti-lipidemic effects generally on alloxan induced diabetic rats. The study’s findings has shown that the plant possess hypoglycaemic and hypolipidaemic property and has supported the traditional use of Luffa cylindrica plant in the management of diabetes and its complications.

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