Naturally occurring antibodies reacting with lipoic acid: screening method, characterization and biochemical interest

Plant lectins are cytotoxic and can be used to select for mutants of animal cells that exhibit structural changes in cell surface carbohydrates reflecting glycosylation defects. We isolated eight lectin mutants of Chinese hamster ovary (CHO) cells that appear to represent three different phenotype classes. These lectin mutants were much more sensitive to the cytotoxic action of normal rabbit serum (NRS) than were the parental cells. This increased cytotoxicity was heat sensitive, specifically absorbed, and inhibited by simple and complex carbohydrates. No killing was observed under conditions in which only the alternate complement pathway was active. An NRS-resistant subclone that was isolated from one lectin mutant was shown to have also regained wild type behavior when tested with the lectins. The possibility that naturally occurring antibodies in rabbit serum are reacting with incomplete carbohydrate chains on the surface of the lectin mutants is discussed.

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Specificity of naturally occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus: Determination by an enzyme linked immunosorbent assay

Immunology Letters ◽

10.1016/0165-2478(86)90026-x ◽

1986 ◽

Vol 12 (5-6) ◽

pp. 251-256 ◽

Cited By ~ 6

Author(s):

Mitsuko Tauchi ◽

Y. Kanai ◽

H. Hashimoto ◽

S. Hirose

Keyword(s):

Systemic Lupus Erythematosus ◽

Immunosorbent Assay ◽

Lupus Erythematosus ◽

Enzyme Linked Immunosorbent Assay ◽

Systemic Lupus ◽

Naturally Occurring ◽

Naturally Occurring Antibodies

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Association of naturally occurring antibodies to β-amyloid with cognitive decline and cerebral amyloidosis in Alzheimer’s disease

Science Advances ◽

10.1126/sciadv.abb0457 ◽

2021 ◽

Vol 7 (1) ◽

pp. eabb0457

Author(s):

Yu-Hui Liu ◽

Jun Wang ◽

Qiao-Xin Li ◽

Christopher J. Fowler ◽

Fan Zeng ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Cognitive Decline ◽

Plasma Levels ◽

Imaging Biomarkers ◽

Amino Terminus ◽

Cross Sectional ◽

Naturally Occurring ◽

Β Amyloid ◽

Naturally Occurring Antibodies

The pathological relevance of naturally occurring antibodies to β-amyloid (NAbs-Aβ) in Alzheimer’s disease (AD) remains unclear. We aimed to investigate their levels and associations with Aβ burden and cognitive decline in AD in a cross-sectional cohort from China and a longitudinal cohort from the Australian Imaging, Biomarkers and Lifestyle (AIBL) study. NAbs-Aβ levels in plasma and cerebrospinal fluid (CSF) were tested according to their epitopes. Levels of NAbs targeting the amino terminus of Aβ increased, and those targeting the mid-domain of Aβ decreased in both CSF and plasma in AD patients. Higher plasma levels of NAbs targeting the amino terminus of Aβ and lower plasma levels of NAbs targeting the mid-domain of Aβ were associated with higher brain amyloidosis at baseline and faster cognitive decline during follow-up. Our findings suggest a dynamic response of the adaptive immune system in the progression of AD and are relevant to current passive immunotherapeutic strategies.

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Differential stromal reprogramming in benign and malignant naturally occurring canine mammary tumours identifies disease-promoting stromal components

10.1101/783621 ◽

2019 ◽

Author(s):

Parisa Amini ◽

Sina Nassiri ◽

Alexandra Malbon ◽

Enni Markkanen

Keyword(s):

Screening Method ◽

Malignant Tumours ◽

Differential Abundance ◽

Naturally Occurring ◽

Mammary Tumours ◽

Ffpe Specimen ◽

Summary Statement ◽

Benign Tumours ◽

Laser Capture ◽

Laser Capture Microdissected

AbstractThe importance of cancer-associated stroma (CAS) for initiation and progression of cancer is well accepted. However, as stromal changes in benign forms of naturally occurring tumours are poorly understood, it remains unclear how CAS from benign and malignant tumours compare. Spontaneous canine mammary tumours are viewed as excellent models of human mammary carcinomas (mCA). We have recently reported highly conserved stromal reprogramming between canine and human mCA based on transcriptome analysis of laser-capture-microdissected FFPE specimen. To identify stromal changes between benign and malignant mammary tumours, we have analysed CAS and matched normal stroma from 13 canine mammary adenomas and compared them to 15 canine mCA. Our analyses revealed distinct stromal reprogramming even in small benign tumours. While similarities in stromal reprogramming exist, the CAS signature clearly distinguished adenomas from mCA, suggesting that it may reliably discriminate between benign and malignant tumours. We identified strongly discriminatory genes and found strong differential enrichment in several hallmark signalling pathways between benign and malignant CAS. The distinction between CAS from adenoma and mCA was further substantiated by differential abundance in cellular composition. Finally, to determine key players in CAS reprograming between adenomas and mCA, a network-based gene screening method identified modules of co-expressing genes with distinct expression profile in benign and malignant CAS, and revealed several hub genes as potential molecular drivers in CAS. Given the relevance of canine CAS as a model for the human disease, our approach identifies potential stromal drivers of tumour malignancy with implications for human mCA.Summary statementRNAsequencing-based analysis of stromal reprogramming between benign and malignant naturally occurring canine mammary tumours identifies potential molecular drivers in cancer-associated stroma that support tumour growth and malignancy.

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The Lutheran Blood Groups: A Second Example of Anti-Lub and Three Further Examples of Anti-Lua

Blood ◽

10.1182/blood.v12.11.998.998 ◽

1957 ◽

Vol 12 (11) ◽

pp. 998-1003 ◽

Cited By ~ 18

Author(s):

TIBOR J. GREENWALT ◽

THOMAS SASAKI

Keyword(s):

Single Dose ◽

Blood Group ◽

Blood Donors ◽

Double Dose ◽

Transfusion Reaction ◽

Her Family ◽

Naturally Occurring ◽

Hemolytic Transfusion Reaction ◽

Naturally Occurring Antibodies ◽

Immune Antibody

Abstract 1. An example of the blood group antibody, anti-Lub, was found in a patient who had a mild hemolytic transfusion reaction. It was shown to possess the characteristics of an immune antibody and to be able to distinguish between a single dose and a double dose of the Lub gene. 2. Three new examples of the antibody, anti-Lua, are presented. All of them were found in normal blood donors and have properties which indicate that they are naturally occurring antibodies. Dr. R. R. Race and Dr. R. Sanger confirmed the presence of anti-Lub in Mrs. S.’s serum, and studied other members of her family and the three anti-Lua sera. We are grateful to them for many favors and their kind encouragement. We are obligated to Miss Marie Cutbush for making available the LuaLua cells from Mrs. R. and her sister, and for a supply of anti-Lub serum. Thanks are due to Dr. A. E. Mourant who furnished our original supply of anti-Lua serum and to Dr. Philip Levine for the anti-Tja and anti-Vel sera. We are indebted to Dr. J. M. Fine of Milwaukee for permission to study Mrs. S. and to the patient and her family for their cooperation. The sera from 18,613 blood donors were studied by Betty McCarthy, Rosemary Polka, Pearl Lemke, Agnes Molnar, Jeannette Flagstadt and Betty Hutter.

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Naturally Occurring Antibodies (NAbs)

10.1007/978-1-4614-3461-0 ◽

2012 ◽

Cited By ~ 10

Keyword(s):

Naturally Occurring ◽

Naturally Occurring Antibodies

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The Immunomodulatory Effect of Alpha-Lipoic Acid in Autoimmune Diseases

BioMed Research International ◽

10.1155/2019/8086257 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Wei Liu ◽

Lian-jie Shi ◽

Sheng-guang Li

Keyword(s):

Autoimmune Diseases ◽

Lipoic Acid ◽

Human Body ◽

Innate Immune ◽

Immunomodulatory Effect ◽

Alpha Lipoic Acid ◽

Immunomodulatory Effects ◽

Immune Systems ◽

Beneficial Effects ◽

Naturally Occurring

Αlpha-lipoic acid is a naturally occurring antioxidant in human body and has been widely used as an antioxidant clinically. Accumulating evidences suggested that α-lipoic acid might have immunomodulatory effects on both adaptive and innate immune systems. This review focuses on the evidences and potential targets involved in the immunomodulatory effects of α-lipoic acid. It highlights the fact that α-lipoic acid may have beneficial effects in autoimmune diseases once the immunomodulatory effects can be confirmed by further investigation.

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