Ovarian development and pteridine accumulation for age determination in the blowfly Lucilia sericata

1991 ◽  
Vol 37 (12) ◽  
pp. 863-868 ◽  
Author(s):  
R. Wall ◽  
P.A. Langley ◽  
K.L. Morgan
Keyword(s):  
Ovarian Development ◽  
Age Determination ◽  
Lucilia Sericata

scholarly journals Age determination of the adult blow fly Lucilia sericata (Diptera: Calliphoridae) through quantitative pteridine fluorescence analysis

2020 ◽  
Vol 16 (4) ◽  
pp. 641-648
Author(s):  
Ronja Estévez Dimitrov ◽  
Jens Amendt ◽  
Florian Rothweiler ◽  
Richard Zehner
Keyword(s):  
Age Estimation ◽  
Age Determination ◽  
Fluorescence Analysis ◽  
Lucilia Sericata ◽  
Head Width ◽  
Blow Fly ◽  
Fluorescence Level ◽  
Juvenile Stages ◽  
Set Up

AbstractDetermination of a minimal postmortem interval via age estimation of necrophagous diptera has been restricted to the juvenile stages and the time until emergence of the adult fly, i.e. up until 2–6 weeks depending on species and temperature. Age estimation of adult flies could extend this period by adding the age of the fly to the time needed for complete development. In this context pteridines are promising metabolites, as they accumulate in the eyes of flies with increasing age. We studied adults of the blow fly Lucilia sericata at constant temperatures of 16 °C and 25 °C up to an age of 25 days and estimated their pteridine levels by fluorescence spectroscopy. Age was given in accumulated degree days (ADD) across temperatures. Additionally, a mock case was set up to test the applicability of the method. Pteridine increases logarithmically with increasing ADD, but after 70–80 ADD the increase slows down and the curve approaches a maximum. Sex had a significant impact (p < 4.09 × 10−6) on pteridine fluorescence level, while body-size and head-width did not. The mock case demonstrated that a slight overestimation of the real age (in ADD) only occurred in two out of 30 samples. Age determination of L. sericata on the basis of pteridine levels seems to be limited to an age of about 70 ADD, but depending on the ambient temperature this could cover an extra amount of time of about 5–7 days after completion of the metamorphosis.

Attraction of the sheep blowfly, Lucilia sericata (Diptera: Calliphoridae) to carrion bait in the field

1998 ◽  
Vol 88 (6) ◽  
pp. 611-616 ◽  
Author(s):  
P. Fisher ◽  
R. Wall ◽  
J.R. Ashworth
Keyword(s):  
Interspecific Competition ◽  
Competitive Ability ◽  
Ovarian Development ◽  
Field Trials ◽  
Lucilia Sericata ◽  
General Relationship ◽  
Pioneer Species ◽  
Sodium Sulphide ◽  
Significant Difference ◽  
Evolutionary Progression

AbstractThe facultative ectoparasite, Lucilia sericata (Meigen) has been described as a pioneer colonizer of carrion. This behaviour is believed to enable it to escape the intense interspecific competition which can occur between dipterous larvae within carrion. Such escape is necessary as this fly species has been thought to have reduced competitive ability, resulting from its evolutionary progression towards parasitism. In the work reported here, the attraction of L. sericata to carrion bait at different stages of decomposition (fresh to 28 days old) was investigated in the field. Trials were run over three years, with carrion bait presented in the presence and absence of sodium sulphide. Decomposition had a significant effect on the numbers of L. sericata caught; more decomposed bait caught greater numbers of L. sericata than fresh bait. However, this effect was not apparent if sodium sulphide was added to the carrion. With sodium sulphide added, there was no significant difference in the attraction of female L. sericata, regardless of bait age. There was no general relationship between carrion bait age and the number of female L. sericata caught in different stages of ovarian development. The present data, therefore, suggest that L. sericata does not act exclusively as a pioneer species. Furthermore, this work suggests that, if used with sodium sulphide, the stage of carrion bait decomposition does not significantly modify carrion bait attractiveness.

Bone age determination using dual-energy X-ray absorptiometry

2017 ◽  
Author(s):  
Khalaf Alshamrani ◽  
Amaka Offiah ◽  
Elzene kruger
Keyword(s):  
Age Determination ◽  
Bone Age ◽  
Dual Energy ◽  
X Ray

BMP15 gene dosage as a relevant X-linked determinant of ovarian development and function

2014 ◽  
Author(s):  
Raffaella Rossetti ◽  
Irene Negri ◽  
Chiara Castronovo ◽  
Palma Finelli ◽  
Luca Persani
Keyword(s):  
Ovarian Development ◽  
Gene Dosage ◽  
And Function

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

Molecular basis of ovarian development and function

2002 ◽  
Vol 7 (1-3) ◽  
pp. d2006 ◽  
Author(s):  
Barbara Vanderhyden
Keyword(s):  
Molecular Basis ◽  
Ovarian Development ◽  
And Function

Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

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