scholarly journals Development and application of Raman microspectroscopic and Raman imaging techniques for cell biological studies

1995 ◽  
Vol 347 ◽  
pp. 477-483 ◽  
Author(s):  
G.J Puppels ◽  
T.C.Bakker Schut ◽  
N.M Sijtsema ◽  
M Grond ◽  
F Maraboeuf ◽  
...  
Keyword(s):  
Imaging Techniques ◽  
Raman Imaging ◽  
Biological Studies

Raman Imaging of Small Biomolecules

2019 ◽  
Vol 48 (1) ◽  
pp. 347-369 ◽  
Author(s):  
Yihui Shen ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Imaging Techniques ◽  
Living Cells ◽  
Raman Imaging ◽  
Biological Processes ◽  
Comprehensive Knowledge ◽  
Contrast Mechanisms ◽  
Recent Developments ◽  
Imaging Methodology ◽  
Small Biomolecules ◽  
Microscopic Techniques

Imaging techniques greatly facilitate the comprehensive knowledge of biological systems. Although imaging methodology for biomacromolecules such as protein and nucleic acids has been long established, microscopic techniques and contrast mechanisms are relatively limited for small biomolecules, which are equally important participants in biological processes. Recent developments in Raman imaging, including both microscopy and tailored vibrational tags, have created exciting opportunities for noninvasive imaging of small biomolecules in living cells, tissues, and organisms. Here, we summarize the principle and workflow of small-biomolecule imaging by Raman microscopy. Then, we review recent efforts in imaging, for example, lipids, metabolites, and drugs. The unique advantage of Raman imaging has been manifested in a variety of applications that have provided novel biological insights.

High-Fidelity Raman Imaging Spectrometry: A Rapid Method Using an Acousto-Optic Tunable Filter

1992 ◽  
Vol 46 (8) ◽  
pp. 1211-1216 ◽  
Author(s):  
Patrick J. Treado ◽  
Ira W. Levin ◽  
E. Neil Lewis
Keyword(s):  
Raman Spectra ◽  
Protein Interactions ◽  
Imaging Techniques ◽  
Computer Control ◽  
Tunable Filter ◽  
Raman Imaging ◽  
Spectral Interval ◽  
High Fidelity ◽  
Spatially Resolved ◽  
Image Pixels

In this communication, we describe a technique for obtaining high-fidelity Raman images and Raman spectra. The instrumentation provides the ability to rapidly collect large-format images with the number of image pixels limited only by the number of detector elements in the silicon charge-coupled device (CCD). Wavelength selection is achieved with an acousto-optic tunable filter (AOTF), which maintains image fidelity while providing spectral selectivity. Under computer control the AOTF is capable of µs tuning speeds within the operating range of the filter (400–1900 nm). The AOTF is integrated with the CCD and holographic Raman filters to comprise an entirely solid-state Raman imager containing no moving parts. In operation, the AOTF is placed in front of the CCD and tuned over the desired spectral interval. The two-dimensional CCD detector is employed as a true imaging camera, providing a full multichannel advantage over competitive Raman imaging techniques. Images and spectra are presented of a mixture of dipalmitoylphosphatidylcholine (DPPC) and L-asparagine, which serves as a model system for the study of both lipid/peptide and lipid/protein interactions in intact biological materials. The Raman images are collected in only several seconds and indicate the efficacy of this rapid technique for discriminating between multiple components in complex matrices. Additionally, high-quality Raman spectra of the spatially resolved microscopic regions are easily obtained.

Direct Raman imaging techniques for study of the subcellular distribution of a drug

2002 ◽  
Vol 41 (28) ◽  
pp. 6006 ◽  
Author(s):  
Jian Ling ◽  
Steven D. Weitman ◽  
Michael A. Miller ◽  
Rodney V. Moore ◽  
Alan C. Bovik
Keyword(s):  
Subcellular Distribution ◽  
Imaging Techniques ◽  
Raman Imaging

scholarly journals Nonlinear plasmonic imaging techniques and their biological applications

Nanophotonics ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 31-49 ◽  
Author(s):  
Gitanjal Deka ◽  
Chi-Kuang Sun ◽  
Katsumasa Fujita ◽  
Shi-Wei Chu
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Imaging Techniques ◽  
Metal Nanostructures ◽  
Special Focus ◽  
Biological Applications ◽  
Minimal Invasiveness ◽  
Biological Studies ◽  
Coherent Raman ◽  
Nonlinear Imaging

AbstractNonlinear optics, when combined with microscopy, is known to provide advantages including novel contrast, deep tissue observation, and minimal invasiveness. In addition, special nonlinearities, such as switch on/off and saturation, can enhance the spatial resolution below the diffraction limit, revolutionizing the field of optical microscopy. These nonlinear imaging techniques are extremely useful for biological studies on various scales from molecules to cells to tissues. Nevertheless, in most cases, nonlinear optical interaction requires strong illumination, typically at least gigawatts per square centimeter intensity. Such strong illumination can cause significant phototoxicity or even photodamage to fragile biological samples. Therefore, it is highly desirable to find mechanisms that allow the reduction of illumination intensity. Surface plasmon, which is the collective oscillation of electrons in metal under light excitation, is capable of significantly enhancing the local field around the metal nanostructures and thus boosting up the efficiency of nonlinear optical interactions of the surrounding materials or of the metal itself. In this mini-review, we discuss the recent progress of plasmonics in nonlinear optical microscopy with a special focus on biological applications. The advancement of nonlinear imaging modalities (including incoherent/coherent Raman scattering, two/three-photon luminescence, and second/third harmonic generations that have been amalgamated with plasmonics), as well as the novel subdiffraction limit imaging techniques based on nonlinear behaviors of plasmonic scattering, is addressed.

Synthesis of deuterated γ-linolenic acid and application for biological studies: metabolic tuning and Raman imaging

2021 ◽  
Author(s):  
Kosuke Dodo ◽  
Ayato Sato ◽  
Yuki Tamura ◽  
Syusuke Egoshi ◽  
Koichi Fujiwara ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Raman Imaging ◽  
Metabolic Inhibition ◽  
Mechanistic Studies ◽  
Selective Cytotoxicity ◽  
Dual Functional ◽  
Biological Studies ◽  
Tumor Selective

γ-Linolenic acid (GLA) is reported to show tumor-selective cytotoxicity via unidentified mechanisms. We introduced deuterium into GLA as a dual functional tag for metabolic inhibition and Raman imaging and applied for mechanistic studies.

scholarly journals Confocal microscopy on the beamline: novel three-dimensional imaging and sample positioning

2012 ◽  
Vol 45 (5) ◽  
pp. 936-943 ◽  
Author(s):  
I. Khan ◽  
R. Gillilan ◽  
I. Kriksunov ◽  
R. Williams ◽  
W. R. Zipfel ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Protein Crystal ◽  
Protein Crystals ◽  
Wide Field ◽  
Three Dimensional Imaging ◽  
Biological Studies ◽  
Confocal Optics

Confocal microscopy, a technique that has been extensively applied in cellular biological studies, may also be applied to the visualization and three-dimensional imaging of protein crystals at high resolution on synchrotron beamlines. Protein crystal samples are examined using a commercially available confocal microscope adapted for cryogenic use. A preliminary test using a custom confocal design adapted for beamline use is also presented. The confocal optics configuration is compatible with nonlinear imaging techniques such as two-photon excited fluorescence imaging and second harmonic generation. The possibilities of this method are explored using two modes: fluorescence and reflection confocal. In fluorescence mode, small amounts of dye are introduced into the crystal through soaking or growth conditions. Under such conditions, protein crystals are easily resolved from salts and amorphous precipitates, which do not generally take up dye. Reflection mode, which does not require dye, still exhibits greater resolution and sensitivity to surface detail than conventional wide-field microscopy as a result of the confocal optics configuration. The inherent three-dimensional nature of the method means that on-axis sample views (along the direction of the X-ray beam) can be reconstructed from an off-axis configuration, simplifying the beamline setup and providing uniquely detailed views of cryogenically cooled crystals.

scholarly journals Raman Imaging of Nanocarriers for Drug Delivery

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 341 ◽  
Author(s):  
Sally Vanden-Hehir ◽  
William Tipping ◽  
Martin Lee ◽  
Valerie Brunton ◽  
Anna Williams ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Imaging Techniques ◽  
Raman Imaging ◽  
The Body ◽  
Label Free ◽  
Dosing Strategy ◽  
Pharmaceutical Agents

The efficacy of pharmaceutical agents can be greatly improved through nanocarrier delivery. Encapsulation of pharmaceutical agents into a nanocarrier can enhance their bioavailability and biocompatibility, whilst also facilitating targeted drug delivery to specific locations within the body. However, detailed understanding of the in vivo activity of the nanocarrier-drug conjugate is required prior to regulatory approval as a safe and effective treatment strategy. A comprehensive understanding of how nanocarriers travel to, and interact with, the intended target is required in order to optimize the dosing strategy, reduce potential off-target effects, and unwanted toxic effects. Raman spectroscopy has received much interest as a mechanism for label-free, non-invasive imaging of nanocarrier modes of action in vivo. Advanced Raman imaging techniques, including coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), are paving the way for rigorous evaluation of nanocarrier activity at the single-cell level. This review focuses on the development of Raman imaging techniques to study organic nanocarrier delivery in cells and tissues.

scholarly journals Selective-sampling Raman Imaging techniques for ex-vivo assessment of surgical margins in cancer surgery

The Analyst ◽  
2021 ◽  
Author(s):  
Maria Giovanna Lizio ◽  
Radu Boitor ◽  
Ioan Notingher
Keyword(s):  
Gold Standard ◽  
Ex Vivo ◽  
Imaging Techniques ◽  
Standard Technique ◽  
Cancer Surgery ◽  
Raman Imaging ◽  
Surgical Margins ◽  
Complete Excision ◽  
Selective Sampling ◽  
Gold Standard Technique

One of the main challenges in cancer surgery is to ensure the complete excision of the tumour while sparing as much healthy tissue as possible. Histopathology, the gold-standard technique used...

Utilization of Bio-Imaging in Cancer Studies

2021 ◽  
pp. 534-540
Author(s):  
Muneesh Kumar Barman ◽  
Manjita Srivastava ◽  
Meenakshi Singh ◽  
Khalid Umar Fakhri ◽  
Kailash Chand ◽  
...  
Keyword(s):  
Cancer Research ◽  
Cancer Cells ◽  
Cancer Diagnosis ◽  
Imaging Techniques ◽  
Ct Scans ◽  
Biological Studies ◽  
Bio Imaging ◽  
Cancer Studies ◽  
Precise Interpretation

Biological studies have always relied on visual data and its precise interpretation. Bio-imaging is an integral part of cancer research as well as the diagnosis and treatment of various cancers. Cancer research employs the various bio-imaging techniques of fluorescence microscopy like confocal microscopy, FRET, FRAP, TPEF, SGH, etc. to study the complexity and characteristics of different cancer cells. The development of live-cell imaging has also helped in understanding the important biological processes which differentiate cancer cells from their environment. Advancement in the field of cancer diagnosis has taken place with the development of sophisticated radiology techniques like MRI, CT scans, and FDG-PET. Also, the development of novel nanotechnology-based probes has improved the quality of both cancer research and diagnosis. In this chapter, the authors summarize some of the bio-imaging techniques which are being used in the field of cancer studies.

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