The authors aim to describe valuable information and experimental reviews that may help to develop and design different formulation, which can boost up the overall efficiency of the final product. Further, they explained the overall efficiency, method of preparation, target delivery approaches, drawbacks, and other characteristics in relation to lipids, peptides, polymers, and vaccines. In addition, they also propose to uncover the physico-chemical properties, in-process manufacturing issues, and external factors that influence the fate of a medicine. That major includes the excipients, method of preparation, dose, delivery route, chemical and biological properties, drug-drug interaction, drug-body interaction, patient compliance, modifications in lipid based nano-vectors, polymer-mediated delivery systems, conjugate delivery systems, and others. In conclusion, by the end of this chapter, the authors are able to explain a robust mode of delivering active constituents more safely and economically to the target site by showing maximum bioavailability.