Microfilaraemia, serum antibody and development of clinical disease in microfilaraemic subjects infected with Wuchereria bancrofti and treated with diethylcarbamazine citrate

1989 ◽  
Vol 83 (3) ◽  
pp. 384-388 ◽  
Author(s):  
Senarath Dissanayake
Keyword(s):  
Serum Antibody ◽  
Wuchereria Bancrofti ◽  
Clinical Disease ◽  
Diethylcarbamazine Citrate

scholarly journals Differential Effects of Influenza Virus NA, HA Head, and HA Stalk Antibodies on Peripheral Blood Leukocyte Gene Expression during Human Infection

mBio ◽  
2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Kathie-Anne Walters ◽  
Ruoqing Zhu ◽  
Michael Welge ◽  
Kelsey Scherler ◽  
Jae-Keun Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Severity ◽  
Peripheral Blood ◽  
Serum Antibody ◽  
Peripheral Blood Leukocyte ◽  
Global Gene Expression ◽  
Clinical Disease ◽  
Viral Shedding ◽  
Antibody Titers ◽  
Blood Leukocyte

ABSTRACT In this study, we examined the relationships between anti-influenza virus serum antibody titers, clinical disease, and peripheral blood leukocyte (PBL) global gene expression during presymptomatic, acute, and convalescent illness in 83 participants infected with 2009 pandemic H1N1 virus in a human influenza challenge model. Using traditional statistical and logistic regression modeling approaches, profiles of differentially expressed genes that correlated with active viral shedding, predicted length of viral shedding, and predicted illness severity were identified. These analyses further demonstrated that challenge participants fell into three peripheral blood leukocyte gene expression phenotypes that significantly correlated with different clinical outcomes and prechallenge serum titers of antibodies specific for the viral neuraminidase, hemagglutinin head, and hemagglutinin stalk. Higher prechallenge serum antibody titers were inversely correlated with leukocyte responsiveness in participants with active disease and could mask expression of peripheral blood markers of clinical disease in some participants, including viral shedding and symptom severity. Consequently, preexisting anti-influenza antibodies may modulate PBL gene expression, and this must be taken into consideration in the development and interpretation of peripheral blood diagnostic and prognostic assays of influenza infection. IMPORTANCE Influenza A viruses are significant human pathogens that caused 83,000 deaths in the United States during 2017 to 2018, and there is need to understand the molecular correlates of illness and to identify prognostic markers of viral infection, symptom severity, and disease course. Preexisting antibodies against viral neuraminidase (NA) and hemagglutinin (HA) proteins play a critical role in lessening disease severity. We performed global gene expression profiling of peripheral blood leukocytes collected during acute and convalescent phases from a large cohort of people infected with A/H1N1pdm virus. Using statistical and machine-learning approaches, populations of genes were identified early in infection that correlated with active viral shedding, predicted length of shedding, or disease severity. Finally, these gene expression responses were differentially affected by increased levels of preexisting influenza antibodies, which could mask detection of these markers of contagiousness and disease severity in people with active clinical disease.

Lymphatic filariasis in the coastal areas of Digha, West Bengal, India

2007 ◽  
Vol 37 (3) ◽  
pp. 136-139 ◽  
Author(s):  
G Chandra ◽  
S N Chatterjee ◽  
S Das ◽  
N Sarkar
Keyword(s):  
Lymphatic Filariasis ◽  
Human Blood ◽  
West Bengal ◽  
Wuchereria Bancrofti ◽  
Coastal Areas ◽  
Disease Rate ◽  
Blood Samples ◽  
Human Blood Index ◽  
Blood Index ◽  
Diethylcarbamazine Citrate

The state of West Bengal, India, has a long coastline with the Bay of Bengal. No information exists regarding filarial epidemiology and its vector in these coastal areas. The present study was designed to assess the epidemiology of lymphatic filariasis and the role of available mosquitoes as its vector in eight coastal villages around Digha, West Bengal. Night blood samples of 4016 individuals were collected and each of them was examined clinically for any manifestations of the disease. Overall, microfilaria rate, mean microfilarial density and disease rate were 9.06%, 8.63% and 7.72%, respectively. The causative parasite was identified as Wuchereria bancrofti and Culex quinquefasciatus was incriminated as the vector responsible. Vector infection and infectivity rates were assessed to be 12.5% and 0.73%, respectively. The human blood index of human-house-frequenting vector population was 70%. Vector density, vector infection, infectivity rates and human blood index were higher in the rainy season in the study area. Overall, the filarial situation was bad and, as a measure, single-dose diethylcarbamazine citrate (6 mg/kg body weight) treatment was given to all the microfilariaemic patients. Night blood samples of the treated individuals were tested for microfilariae on days 10 and 365, which revealed interesting results.

Lymphatic filariasis

2020 ◽  
pp. 1487-1495
Author(s):  
Richard Knight
Keyword(s):  
At Risk ◽  
Slave Trade ◽  
Social Stigma ◽  
Wuchereria Bancrofti ◽  
Clinical Disease ◽  
Animal Reservoir ◽  
Nematode Parasites ◽  
Control Programmes ◽  
Human Infections ◽  
Systematic Control

Wuchereria bancrofti, Brugia malayi, and B. timori are mosquito-borne lymphatic-dwelling nematode parasites that are important causes of morbidity, disability, and social stigma in tropical and subtropical countries. The total population at risk is now estimated to be 856 million in some 52 countries where these infections are endemic. In 2000 before systematic control programmes 120 million people were infected, of whom about 40 million had clinical disease and some 80 million had hidden lymphatic damage. Bancroftian filariasis due to W. bancrofti, which has no animal reservoir, accounts for 90% human infections worldwide; it was introduced into the Americas from Africa by the Atlantic slave trade.

FAKTOR-FAKTOR YANG MEMPENGARUHI KETIDAKPATUHAN MINUM OBAT SEBAGAI UPAYA PENCEGAHAN FILARIASIS DI KOTA PEKALONGAN

2019 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Harfaina Harfaina ◽  
Suharyo Hadisaputro ◽  
Djoko Trihadi Lukmono ◽  
Mateus Sakundarno
Keyword(s):  
Self Efficacy ◽  
Random Sampling ◽  
Social Stigma ◽  
Brugia Malayi ◽  
Sampling Technique ◽  
Wuchereria Bancrofti ◽  
Drug Prevention ◽  
Permanent Disability ◽  
Social Environmental ◽  
Cluster Random Sampling

Filariasis adalah penyakit infeksi yang disebabkan oleh cacing Wuchereria Bancrofti, Brugia Malayi, dan Brugia Timori yang menyebabkan cairan limfe tidak dapat tersalurkan dengan baik sehingga menyebabkan pembengkakan pada tungkai dan lengan. Meskipun tidak ada penyebab kematian tetapi menyebabakan cacat permanen dan stigma sosial. Eliminasi Filariasis dilakukan dengan Program Pengobatan Massal ke seluruh penduduk di daerah endemis setahun sekali selama 5 tahun. Keberhasilan program ini memerlukan kepatuhan minum obat pencegahan filariasis. Tujuan penelitian ini untuk mengetahui faktor-faktor yang mempengaruhi ketidakpatuhan minum obat sebagai upaya pencegahan filariasis. Penelitian ini Populasi dalam penelitian ini adalah penduduk berusia 15-65 tahun di dua kelurahan endemis yaitu kelurahan kuripan kertoharjo dan kelurahan jenggot selama mei-juli 2018. Sampel dalam penelitian ini 80 kasus dan 80 kontrol dengan teknik cluster random sampling. Variabel yang terbukti berpengaruh yaitu persepsi kerentanan negatif (OR=4,093) 95%CI=1,356-12,350 dan self efficacy negatif (OR=30,298) 95%CI=8,986-102,156. Persepsi kerentanan negatif dan self efficacy negatif merupakan faktor perilaku yang mempengaruhi ketidakpatuhan minum obat pencegahan filariasis. Diharapkan ada penelitian lanjutan tentang ketidakpatuhan minum obat pencegahan filariasis bukan berwujud persepsi tetapi dengan pengukuran faktor lingkungan sosial secara objektif dengan melakukan intervensi berupa perubahan perilaku.   Kata kunci : Filariasis, Ketidakpatuhan, Minum Obat, Mix Method   FACTORS THAT INFLUENCE DRINKING DRUG PREVENTION NON COMPLIANCE OF FILARIASIS IN PEKALONGAN CITY   ABSTRACT Filariasis is an infectious disease caused by worms Wuchereria Bancrofti, Brugia Malayi, and Brugia Timori, adult worm lives and damage reulting in blockage of lymph channels, causing swelling of the legs and arms. Although no cause of death but causes permanent disability and social stigma. Filariasis elimination done with the Mass Treatment Program to the entire population in endemic areas a year for 5 year. Succesfully this program required a medication adherence. The purpose of this study was to determine the factors that influence drug disobedience as an effort to prevent filariasis. This study uses a mix method. The population in this study were residents aged 15-65 years in two endemic villages, namely kuripan kertoharjo and jenggot villages during May-July 2018. Samples in this study were 80 cases and 80 controls with cluster random sampling technique. Variables that proved influential were perceptions of negative vulnerability (OR = 4,093) 95% CI = 1,356-12,350 and negative self efficacy (OR = 30,298) 95% CI = 8,986-102,156. Negative vulnerability perceptions and negative self efficacy are behavioral factors that influence non-compliance with filariasis prevention drugs. It is expected that further research on non-compliance with taking drugs to prevent filariasis is not a form of perception but objective measurement of social environmental factors by intervening in the form of behavior change.   Keywords: Filariasis, Noncompliance, Medication, Mix Method

TBE in United Kingdom

2020 ◽  
Keyword(s):  
United Kingdom ◽  
Clinical Disease ◽  
England And Wales ◽  
Probable Case ◽  
The United Kingdom ◽  
South West ◽  
Louping Ill ◽  
The Uk ◽  
North And South ◽  
South West England

Until 2019, TBE was considered only to be an imported disease to the United Kingdom. In that year, evidence became available that the TBEV is likely circulating in the country1,2 and a first “probable case” of TBE originating in the UK was reported.3 In addition to TBEV, louping ill virus (LIV), a member of the TBEV-serocomplex, is also endemic in parts of the UK. Reports of clinical disease caused by LIV in livestock are mainly from Scotland, parts of North and South West England and Wales.4

Linkage between H. pylori Infection and TNF-α polymorphism in The Pregnant Women

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ghaidaa Raheem Lateef ◽  
Azhar Omaran Al-Thahab
Keyword(s):  
Pregnant Women ◽  
Serum Antibody ◽  
Rapid Test ◽  
Negative Group ◽  
Positive Group ◽  
Gynecology And Obstetrics ◽  
Tnf Α ◽  
Significant Difference ◽  
Pcr Products ◽  
H Pylori

A study was performed on 100 pregnant women in the outpatient department of gynecology and obstetrics of Maternity and Children Hospital in Al-Diwaniya City during the period between (March to September 2016). One hundred blood samples (50 for patients and 50 for control) were collected under the supervision of the treating gynecologist. The detection of Helicobacter. pylori was done by the use of the serum antibody Rapid test. The results showed that 50 (100%) were positive and 50 (100%) were negative for H. pylori in above method.All blood of patients and control samples were used for the extraction of genomic DNA,where the 107 bp PCR product size. Genotyping of the TNF-α-308 SNP (G/A)was performed by restriction fragment length polymorphism PCR (RFLP-PCR). PCR products were digested with restr NcoI iction enzyme. Individuals with the TNF-α-308(GG) homozygote produced digested DNA bands at 80,and 20 bp bp. A heterozygous genotype ofTNF-α-308 (GA)produced 107 bp,80 bp,and 20 bp bands. Individuals with the TNF-α-308 (AA) homozygote genotype had no amplicon digested and generated only one band of 107 bp. There was a significant difference in the frequency of the TNF-α-308(GG)genotype between H. pylori positive group and H. pylori negative group(72%,78% respectively). Also for GA genotype,there was a significant difference between H. pylori positive group and H. pylori negative group(24%,18% respectively). Concerning the frequency of the TNF-α-308 (AA)genotype between H. pylori positive group and H. pylori negative group,there was no significant difference between the two groups.

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