A subacute toxicity study of N-(2-mercaptoethyl) benzenesulfonamide S-(O,O-diisopropyl phosphorodithioate) and phthalimidomethyl-O,O-dimethyl phosphorodithioate with Japanese quail

1965 ◽  
Vol 7 (4) ◽  
pp. 550-558
Author(s):  
Thomas E. Shellenberger ◽  
Gordon W. Newell ◽  
Rita M. Bridgman ◽  
Jack Barbaccia
Keyword(s):  
Japanese Quail ◽  
Toxicity Study ◽  
Subacute Toxicity

Safety Evaluation of Eucalyptus globulus Essential Oils through Acute and Sub-acute Toxicity and Skin Irritation in Mice and Rats

2020 ◽  
Vol 14 (3) ◽  
pp. 187-195
Author(s):  
Berhan Mengiste ◽  
Tizazu Zenebe ◽  
Kassahun Dires ◽  
Ermias Lulekal ◽  
Awol Mekonnen ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oil ◽  
Eucalyptus Globulus ◽  
Biochemical Parameters ◽  
Skin Irritation ◽  
Toxicity Study ◽  
Subacute Toxicity ◽  
Acute Toxicity Study ◽  
Limit Test ◽  
Ointment Formulation

Background: The Eucalyptus globulus extractions have been used by the traditional healers to treat diseases in the study area. Our previous study revealed that the essential oil has antimicrobial and antifungal activity. This study determined phytochemical analysis, skin irritation, acute and subacute toxicity of Eucalyptus globulus essential oil in mice and rats. Methods: The phytochemicals were analyzed using GC-MS mass spectrometry. The acute toxicity study was determined at three dose levels of 1500 mg/kg, 1750mg/kg, and 2000 mg/kg. The essential oil limit test at a dose of 1000 mg/kg was administered to mice for 28 consecutive days for sub-acute toxicity study. The mice mortality, behavioral change, injury and other signs of illness were recorded once daily. Biochemical parameters were evaluated. Liver and kidney were analyzed for histopathological analyses. The 5% ointment formulation was applied to the rat skin to determine skin irritation effects. Results: The Eucalyptus globulus essential oil showed no effect on the mice at a dose of 1500mg/kg and below, but caused signs of toxicity and death at a dose of 1750mg/kg and above compared to the controls (p<0.05). The LD50 value was 1650 mg/kg. There was no significant difference (p > 0.05) in the body weights, gross abnormalities of the organs and biochemical parameters compared to the control at 1000 mg/kg subacute toxicity study. No histopathological changes were detected in the organs tested. The 5% ointment formulation did not show any abnormal skin reaction. Discussion: In the present study, the Eucalyptus globulus essential oil was comparable with other studies in terms of both chemical composition and its effects on sub-acute and topical application. Conclusion: This toxicity study demonstrated that Eucalyptus globulus essential oil is nontoxic at a relatively lower concentration.

scholarly journals Subacute toxicity study in Wistar rats fed with StemEnhanceTM, an extract from Aphanizomenon flos-aquae

2010 ◽  
pp. 125 ◽  
Author(s):  
Dirikolu ◽  
Thushara Chakkath ◽  
Susan Ball-Kell ◽  
Christy Elamma ◽  
Timothy M. Fan ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Toxicity Study ◽  
Subacute Toxicity

scholarly journals Thirteen-week subacute toxicity study of 4-bromophenyl chloromethyl sulfone (BCS) in rats.

1985 ◽  
Vol 10 (4) ◽  
pp. 295-308 ◽  
Author(s):  
Kunitoshi MITSUMORI ◽  
Keizo MAITA ◽  
Tadashi KOSAKA ◽  
Teiji MIYAOKA ◽  
Yasuhiko SHIRASU
Keyword(s):  
Toxicity Study ◽  
Subacute Toxicity

Acute and Subacute toxicity study of Olaquindox by feeding to common carp (Cyprinus carpio L.)

2018 ◽  
Vol 161 ◽  
pp. 342-349 ◽  
Author(s):  
Qian Yang ◽  
Jie He ◽  
Sheng-yu He ◽  
Kai-yu Wang ◽  
Yi Geng ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Toxicity Study ◽  
Subacute Toxicity ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Subacute Toxicity Study of Malic Acid Propane 1,2-diol Copolyester

2003 ◽  
Vol 3 (4) ◽  
pp. 274-282
Author(s):  
M.A. Bakar ◽  
M.A. Islam . ◽  
S. Khatun . ◽  
G. Sadik .
Keyword(s):  
Malic Acid ◽  
Toxicity Study ◽  
Subacute Toxicity

scholarly journals Subacute Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract Induced Liver and Kidney Toxicities in ICR Mice

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2631
Author(s):  
Abdullahi Aliyu ◽  
Mohd Rosly Shaari ◽  
Nurul Syahirah Ahmad Sayuti ◽  
Mohd Farhan Hanif Reduan ◽  
Shanmugavelu Sithambaram ◽  
...  
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Lethal Dose ◽  
Toxicity Study ◽  
Oral Gavage ◽  
Subacute Toxicity ◽  
Icr Mice ◽  
Clinacanthus Nutans ◽  
Group B ◽  
Ethanolic Leaf Extract

This study investigated the leaves of Clinacanthus nutans for its bioactive compounds and acute and subacute toxicity effects of C. nutans ethanolic leaf extract (CELE) on blood, liver and kidneys of ICR mice. A total of 10 8-week-old female mice were divided into groups A (control) and B (2000 mg/kg) for the acute toxicity study. A single dose of 2000 mg/kg was administered to group B through oral gavage and mice were monitored for 14 days. In the subacute toxicity study, mice were divided into five groups: A (control), B (125 mg/kg), C (250 mg/kg), D (500 mg/kg) and E (1000 mg/kg). The extract was administered daily for 28 days via oral gavage. The mice were sacrificed, and samples were collected for analyses. Myricetin, orientin, isoorientin, vitexin, isovitexin, isookanin, apigenin and ferulic acid were identified in the extract. Twenty-eight days of continuous oral administration revealed significant increases (p < 0.05) in creatinine, ALT and moderate hepatic and renal necrosis in groups D and E. The study concluded that the lethal dose (LD50) of CELE in mice is greater than 2000 mg/kg and that repeated oral administrations of CELE for 28 days induced hepatic and renal toxicities at 1000 mg/kg in female ICR mice.

scholarly journals Acute and Subacute Toxicity Studies of the Ethyl Acetate Soluble Proanthocyanidins of the Immature Inflorescence of Cocos nucifera L. in Female Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
C. P. Ekanayake ◽  
M. G. Thammitiyagodage ◽  
S. Padumadasa ◽  
B. Seneviratne ◽  
C. Padumadasa ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Wistar Rats ◽  
Cocos Nucifera ◽  
Toxicity Study ◽  
Immature Inflorescence ◽  
Subacute Toxicity ◽  
Toxicity Studies ◽  
Female Wistar Rats

Ayurvedic and traditional medical practitioners of Sri Lanka use the decoction of the immature inflorescence of Cocos nucifera L. (IC) variety aurantiaca for the treatment of menorrhagia. The progestogenic effect of the ethyl acetate soluble proanthocyanidins (EASPA) of the IC in female rats at a dose of 3.5 mg/kg body weight has been reported. Acute and subacute toxicity studies of EASPA of the IC carried out using female Wistar rats according to Organization for Economic Co-operation and Development (OECD) guidelines 423 and 407, respectively, are reported herein. In the acute toxicity study, a single dose of EASPA (2000 mg/kg body weight) was orally administered to rats, which were monitored for 14 days. In the subacute toxicity study, rats were orally administered with EASPA daily for 28 days at doses of 1.75, 3.5, 7, and 14 mg/kg body weight. No rat in either the acute or subacute toxicity study exhibited mortality or clinical signs of toxicity. Further, these rats did not show any significant change in their mean body weight, food, and water intake, haematological and biochemical parameters as well as in the results of their histopathological examinations compared to those of control group rats. According to results of the acute toxicity, the LD50 of EASPA is estimated to be greater than 2000 mg/kg body weight. Considering the results of the subacute toxicity study, the oral administration of EASPA daily for 28 days was well tolerated up to the dose, 14 mg/kg by rats. These results will be useful in the development of a novel therapeutic agent from EASPA of the IC for the treatment of menorrhagia, which incapacitates a considerable proportion of women worldwide.

Subacute toxicity study of CKD-501, a novel thiazolidinedione, after 4-week repeated oral administration in mice

2013 ◽  
Vol 221 ◽  
pp. S255
Author(s):  
Kyoung-Sik Moon ◽  
Jung-Ho Noh ◽  
Yong-Bum Kim ◽  
Eun Ju Jeong ◽  
In-Chang Hwang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Toxicity Study ◽  
Subacute Toxicity

scholarly journals Acute and subacute toxicity profiles on siddha drug Thulasi Ennai in wistar rats

2020 ◽  
Vol 9 (6) ◽  
pp. 403-409
Author(s):  
S Sonitha ◽  
◽  
D Sivaraman ◽  
V Rani ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Medicinal Plants ◽  
Clinical Signs ◽  
Toxicity Study ◽  
Albino Rats ◽  
Physical Parameters ◽  
Histopathological Analysis ◽  
Common Chronic Disease ◽  
Subacute Toxicity

Medicinal plants have been used in traditional medicines for their unmatched availability of bioactive compounds. Asthma is the most common chronic disease among children worldwide. It is ranked 16th among the leading causes of years lived with disability. Medicinal plants have placed a vital role in the siddha system of medicine over centuries to cure acute and chronic illness. The aim of the present study was to investigate toxicity analysis to evaluate safety of the siddha drug Thulasi Ennai in vivo in wistar albino rats. Thulasi Ennai is a polyherbal siddha formulation mentioned in the ancient siddha books and literature, indicated to cure childhood bronchial asthma. In this study, Thulasi Ennai administered orally at a single dose of 2000mg/kg body weight and monitored for 14 days. For subacute toxicity study, Thulasi Ennai was orally administered in different doses of 200,400mg/kg body weight, daily for 28 days. At the end of each study physical parameters, hematological, biochemical and histopathological analysis were evaluated. No animals in each group of acute or subacute toxicity study showed mortality or clinical signs of toxicity throughout the study. Hence, the results of the study indicate a safe toxicological profile of Thulasi Ennai.

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