Minimum inhibitory concentration quality-control guidelines for biapenem, DU-6859a, FK-037, levofloxacin, grepafloxacin, and ceftizoxime when using various national committee for clinical laboratory standards susceptibility test methods

1994 ◽  
Vol 19 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Martha J. Bale ◽  
Ronald N. Jones ◽  
Meridith E. Erwin
Keyword(s):  
Quality Control ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Clinical Laboratory ◽  
Test Methods ◽  
Susceptibility Test ◽  
National Committee

scholarly journals Spectrophotometric Screening of Potent Bactericidal Property of Thevetia Peruviana Schum. Leaf and Fruit Rind Extracts on Clinical and Plant Pathogens

2014 ◽  
Vol 2 (4) ◽  
pp. 451-459 ◽  
Author(s):  
Nazneen Rahman ◽  
Riaz Mahmood ◽  
Haseebur Rahman ◽  
Mir Haris
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Plant Pathogens ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Clinical Laboratory ◽  
Activity Index ◽  
Multi Drug Resistance ◽  
National Committee ◽  
E Coli ◽  
Fruit Rind

The development of multi drug resistance in human and plant pathogens, reconsidering the traditional medicines as antibacterial source and presence of promising phytochemicals in leaf and fruit rind of T. peruviana (S) (Nazneen et al., 2014) prompted the authors to take up the antibacterial evaluation of different extracts. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of various extracts of T. peruviana (S) were measured using methods of National Committee for Clinical Laboratory Standards (NCCLS).The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) spectrophotometric determination of active extracts has found that the MICs of all the active extracts lies between the range of 250 μg to 1250 μg and bactericidal concentration in a range of 500 to 1250 μg. The percentage of inhibition was analysed, where in among all the extracts tested against B. subtilis and E. coli, fruit upper liquid (FUL) exhibited highest inhibition percentage of 33.75% and 30.31 % at 500 μg/ml respectively. Similarly, fruit hexane (FH) extract has the highest inhibition of 15.60% against B. cereus at 750 μg/ml. The Xanthomonas sp. was susceptible to leaf chloroform (LC) with 32.29% of inhibition at 1000 μg/ ml. The activity index and total antimicrobial activity indicates the antibacterial action of extracts.The present investigations have revealed that among the extracts, the fruit rind extracts have most prominent inhibition abilities against tested bacteria, which are validating the use of this plant in traditional system of medicine and this is the first report of exploration of above extracts for their antibacterial activities against B. subtilis, B. cereus, E. coli and Xanthomonas sp. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11206Int J Appl Sci Biotechnol, Vol. 2(4): 451-459 

scholarly journals Impact of Reappraisal of Fluoroquinolone Minimum Inhibitory Concentration Susceptibility Breakpoints in Gram-Negative Bloodstream Isolates

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 189 ◽  
Author(s):  
Stephanie C. Shealy ◽  
Matthew M. Brigmon ◽  
Julie Ann Justo ◽  
P. Brandon Bookstaver ◽  
Joseph Kohn ◽  
...  
Keyword(s):  
Risk Factors ◽  
South Carolina ◽  
Minimum Inhibitory Concentration ◽  
Antimicrobial Resistance ◽  
Inhibitory Concentration ◽  
Clinical Laboratory ◽  
Gram Negative ◽  
Resistance Risk ◽  
Serious Infections ◽  
The Impact

The Clinical Laboratory Standards Institute lowered the fluoroquinolone minimum inhibitory concentration (MIC) susceptibility breakpoints for Enterobacteriaceae and glucose non-fermenting Gram-negative bacilli in January 2019. This retrospective cohort study describes the impact of this reappraisal on ciprofloxacin susceptibility overall and in patients with risk factors for antimicrobial resistance. Gram-negative bloodstream isolates collected from hospitalized adults at Prisma Health-Midlands hospitals in South Carolina, USA, from January 2010 to December 2014 were included. Matched pairs mean difference (MD) with 95% confidence intervals (CI) were calculated to examine the change in ciprofloxacin susceptibility after MIC breakpoint reappraisal. Susceptibility of Enterobacteriaceae to ciprofloxacin declined by 5.2% (95% CI: −6.6, −3.8; p < 0.001) after reappraisal. The largest impact was demonstrated among Pseudomonas aeruginosa bloodstream isolates (MD −7.8, 95% CI: −14.6, −1.1; p = 0.02) despite more conservative revision in ciprofloxacin MIC breakpoints. Among antimicrobial resistance risk factors, fluoroquinolone exposure within the previous 90 days was associated with the largest change in ciprofloxacin susceptibility (MD −9.3, 95% CI: −16.1, −2.6; p = 0.007). Reappraisal of fluoroquinolone MIC breakpoints has a variable impact on the susceptibility of bloodstream isolates by microbiology and patient population. Healthcare systems should be vigilant to systematically adopt this updated recommendation in order to optimize antimicrobial therapy in patients with bloodstream and other serious infections.

scholarly journals Evaluation of a Colorimetric Antifungal Susceptibility Test by Using 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride

2004 ◽  
Vol 48 (11) ◽  
pp. 4457-4459 ◽  
Author(s):  
Jeong Hwan Shin ◽  
Jae-Cheol Choi ◽  
Jeong Nyeo Lee ◽  
Hyung Hoi Kim ◽  
Eun Yup Lee ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Colorimetric Assay ◽  
Antifungal Susceptibility ◽  
Susceptibility Test ◽  
National Committee ◽  
Tetrazolium Chloride ◽  
Antifungal Susceptibility Test

ABSTRACT A colorimetric antifungal susceptibility test was performed using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. Among 24 strains of Candida species, no trailing growth was found. In 22 and 20 strains, the MICs obtained in the colorimetric assay were within two dilutions of those obtained by the National Committee for Clinical Laboratory Standards method for ketoconazole and itraconazole, respectively.

scholarly journals Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of methicillin-resistant Staphylococcus aureus (MRSA) with their antibiogram

1970 ◽  
Vol 6 (1) ◽  
pp. 121-126 ◽  
Author(s):  
MA Islam ◽  
MM Alam ◽  
ME Choudhury ◽  
N Kobayashi ◽  
MU Ahmed
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Methicillin Resistant ◽  
Antibiotic Susceptibility Test ◽  
Mrsa Strains

The minimum inhibitory concentration (MIC) represents the concentration of antimicrobial at which there is complete inhibition of growth of organism. In order to determine the MIC of cloxacillin, 10 MRSA were previously detected from 40 clinical isolates of Staphylococcus aureus by polymerase chain reaction (PCR). Agar plate dilution test was used to determine the MIC of cloxacillin. The clinical samples were collected from Mymensingh Medical College Hospital, Mymensingh. The study was done in the Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh from July 2006 to June 2007. The MIC of the cloxacillin for 5 MRSA strains were ≥32 (mg/ml), for 1 MRSA strain was ≥ 128(mg/ml) and for another 4 MRSA strains were above ≥128 (mg/ml). Antimicrobial susceptibility test of the isolated organisms were done by disc diffusion method. On antibiotic susceptibility test, MRSA strains showed 100% resistant against penicillin, oxacillin, cloxacillin and amoxycillin. Cent per cent susceptibility of MRSA was found against vancomycin, ciprofloxacin, erythromycin, fusidic acid and rifampicin. Key words: Minimum inhibitory concentration (MIC), antibiotic resistance, cloxacillin, methicillin-resistant Staphylococcus aureus (MRSA) DOI = 10.3329/bjvm.v6i1.1350 Bangl. J. Vet. Med. (2008). 6 (1): 121-126

Comparison of the Vitek GPS-TB Card With Disk Diffusion Testing for Predicting the Susceptibility of Enterococci to Vancomycin

1999 ◽  
Vol 123 (7) ◽  
pp. 622-625
Author(s):  
Natalie Williams-Bouyer ◽  
Barbara S. Reisner ◽  
Carla E. Woodmansee ◽  
Pamela S. Falk ◽  
C. Glen Mayhall
Keyword(s):  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Test Methods ◽  
Disk Diffusion ◽  
National Committee ◽  
Repeat Testing ◽  
Disk Diffusion Testing ◽  
Intermediate Results

Abstract Objective.—To compare the ability of the Vitek GPS-TB card with disk diffusion testing for determining the susceptibility of enterococci to vancomycin. Design.—Vitek susceptibility testing was performed using the GPS-TB card and software version R05.03. Disk diffusion susceptibility testing was performed according to National Committee for Clinical Laboratory Standards guidelines. When discrepancies occurred between the interpretation of Vitek and disk diffusion, both tests were repeated and the epsilometer test (E test) and agar screen containing 6 μg/mL vancomycin were performed. Results.—Of 415 isolates tested, 313 were susceptible to vancomycin and 97 were resistant to vancomycin by both test methods. Two isolates were intermediate by Vitek and resistant by disk diffusion, 2 were intermediate by Vitek and susceptible by disk diffusion, and 1 was susceptible by Vitek and intermediate by disk diffusion. All but 1 of these latter 5 isolates (intermediate by Vitek and susceptible by disk diffusion) were available for retesting. On repeat testing, the 2 isolates that were intermediate by Vitek and resistant by disk diffusion were resistant by both methods, the 1 isolate that was intermediate by Vitek and susceptible by disk diffusion was susceptible by both methods, and the isolate that was susceptible by Vitek and intermediate by disk diffusion was also susceptible by both methods. These results were confirmed by E test and agar screen. Conclusion.—We found the results of the GPS-TB card compared well with disk diffusion. However, isolates with intermediate results by Vitek should be retested using another method, such as the E test.

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