REDUCING SUGAR DERIVATIZATION FOR ULTRAVIOLET ABSORPTION DETECTION IN HPLC ANALYSES

ABSTRACT Serum gonadotrophin (PMS) when added to the incubation medium of rat ovary slices increases the amount of Δ4-3-ketosteroids produced. This enhancement is proportional to the logarithm of dose. The ketosteroids were determined by their ultraviolet absorption; paper chromatography has shown that only androst-4-en-3,17-dione is present.

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Interaction effect of rootstocks on gas exchange parameters, biochemical changes and nutrientstatus in Sauvignon Blanc winegrapes

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v3i3.6566 ◽

2014 ◽

Vol 3 (3) ◽

pp. 218-225

Author(s):

R. G. Somkuwar ◽

M. A. Bhange ◽

A. K. Upadhyay ◽

S. D. Ramteke

Keyword(s):

Biochemical Characterization ◽

Reducing Sugar ◽

Wine Grape ◽

Gas Exchange Parameters ◽

Food Material ◽

Total Carbohydrates ◽

Salt Creek ◽

Grape Varieties ◽

Photosynthetic Activities

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.

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Biochemical Profiles of Various Fresh Extracts of Gnaphalium polycaulon Pers

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i04.8540 ◽

2017 ◽

Vol 9 (04) ◽

Author(s):

Shanmugapriya K. ◽

T. Murugan ◽

Thayumanavan Tha

Keyword(s):

Biochemical Analysis ◽

South India ◽

Standard Procedure ◽

Research Work ◽

Reducing Sugar ◽

Total Lipids ◽

Total Proteins ◽

Total Carbohydrates ◽

Biochemical Profiles ◽

First Time

Medicinal plants plays a significant role in the pharmaceutical industry. In present scenario, the need is to explore, identify and utilize this new medicinal plant on one hand and, on the other, to help conserve the existing but threatened species of rare medicinal plant.This present research work was carry out for the first time in South India to analyse and estimate the biochemical profiles of various extracts of fresh parts of Gnaphalium polycaulon pers., plant. The biochemical composition such as total carbohydrates, total proteins, total lipids, total phenols, cholesterol, total chlorophylls and reducing sugar, sterols were estimated using the standard procedure in fresh plant material.The biochemical analysis of Gnaphalium polycaulon plant showed the presence of various phytochemicals. The results of the present study supplement the usage of the studied plant which possesses several bioactive compounds and used as food and also as medicine.The results of present studies demonstrated that Gnaphalium polycaulon plant could be a sourceof valuable information and a guideline for the scientists, researchers in India and also all over the world

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HPLC-UV Method for Determination of Famotidine from Pharmaceutical Products

Revista de Chimie ◽

10.37358/rc.18.2.6093 ◽

2018 ◽

Vol 69 (2) ◽

pp. 297-299

Author(s):

Adriana Nita ◽

Delia Mirela Tit ◽

Lucian Copolovici ◽

Carmen Elena Melinte (Frunzulica) ◽

Dana Maria Copolovici ◽

...

Keyword(s):

Acetic Acid ◽

Quantitative Analysis ◽

Linear Response ◽

Mobile Phase ◽

Acetic Acid Solution ◽

Pharmaceutical Products ◽

Quantification Limit ◽

Validation Parameters ◽

Hplc Analyses

The aim of this study was to develop and validate a rapid, accurate, and exact method for the quantitative determination of famotidine in pharmaceutical products. The HPLC analyses were performed by using a mobile phase containing methanol:1% acetic acid solution=30:7 (v/v), at a flow rate of 0.4 mL/min.The total time of the method was 10 min, and the retention time of famotidine was 4.16 min. The detection was evaluated at l=267 nm. The method has been validated by using different validation parameters. The linear response of the detector for famotidine peak area was observed at concentrations ranging from 0.1 to 0.0001 mg mL-1 , resulting in a correlation coefficient of 0.99998. The values of the detection limit and of the quantification limit are 0.00048 mg mL-1 and 0.00148 mg mL-1, respectively. The method proposed allowed accurate (with a relative error of less than 2%) and precise (RSD values less than 2.0%) determination of famotidine content in pharmaceutical products and can be used for its rapid quantitative analysis.

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Effect of Proteases on the Extraction of Crude Protein and Reducing Sugar in Pollen

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2004.33.8.1353 ◽

2004 ◽

Vol 33 (8) ◽

pp. 1353-1358 ◽

Cited By ~ 4

Keyword(s):

Crude Protein ◽

Reducing Sugar

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Quality Control Parameters of Mandukarni Syrup - A classical memory enhancing dosage form

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v2i4.9328 ◽

2017 ◽

Vol 2 (4) ◽

Author(s):

Almas Tarannum ◽

Prathviraj Puranik ◽

Suma V. Mallya

Keyword(s):

Quality Control ◽

Refractive Index ◽

Specific Gravity ◽

Dosage Form ◽

Reducing Sugar ◽

Standard Protocol ◽

Direct Impact ◽

Control Parameters ◽

Quality Control Parameters ◽

Memory Enhancing

Ayurveda is the healing medicine. Dosha, Dhatu, Mala and Agni play important role in maintaining health of a person, but without equilibrium of Atma, Indriya and Manas they are helpless. Manas is one entity which is the controller of health. There are several herbs which have direct impact on Manas, among them Mandukaparni is one. Swarasa of Mandukaparni is highly effective, but it is not easily available for children. This study is intended to make Syrup form of Mandukaparni and evaluate its pharmacognostical parameters. According to the methodology refractive index, total solids, specific gravity, reducing and non Reducing sugar and HPTLC parameters were assessed. The results were found to be genuine fulfilling the standard protocol. This study is under taken to evaluate the pharmacognostic properties of Mandukaparni syrup.

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