Interleukins-1α and -1β Regulate Interleukin-6 Expression in Leydig and Sertoli cells

1995 ◽  
pp. 367-372 ◽  
Author(s):  
YOSHIHIRO OKUDA ◽  
C. WAYNE BARDIN ◽  
LYANN R. HODGSKIN ◽  
PATRICIA L. MORRIS
Keyword(s):  
Sertoli Cells ◽  
Interleukin 6

scholarly journals 99 Temporal and spatial characterization of interleukin-6 protein in the caprine testis

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 83-83
Author(s):  
Khalda Fadlalla
Keyword(s):  
Sertoli Cells ◽  
Interleukin 6 ◽  
Assisted Reproductive Technologies ◽  
The United States ◽  
Reproductive Technologies ◽  
Testicular Development ◽  
Cell Growth And Differentiation ◽  
And Function ◽  
Reproductive Processes

Abstract The demand for goat products is one of fastest growing segments of livestock production in the United States. Nonetheless, the development of efficient goat breeding stock remains in its infancy. Assisted reproductive technologies (ART) have the potential to enhance reproductive efficiency in goats to rapidly propagate superior genetics. In order to improve success with ART, it is pertinent to understand the factors that contribute to reproductive processes, such as spermatogenesis, as well as the factors that affect the production of viable sperm. Interleukin-6 (IL-6) is a multifunctional cytokine produced mainly by macrophages in response to foreign antigens, pathogens. In recent years, studies showed that mammalian testes produce IL-6. Under in vitro conditions, Sertoli cells secrete IL-6 following stimulation with low levels of testosterone and follicle stimulating hormone in mice. The binding of IL-6 promotes the transcription of genes important to cellular processes, such as cell growth and differentiation, which are critical to testicular function. The overall objective of this study was to characterize IL-6 protein in goat testes during three life stages: neonate (n = 3; 13- 21days), pre-pubertal (n = 3; 110–121 days) and adult (n = 3; 11–12 month) goats. Preliminary data from western blotting revealed that testicular goat tissues express IL-6. Immunohistochemical staining of IL-6 in the peritubular myoid cells and interstitial cells of neonate, pre-pubertal and adult goats; further Sertoli cells express IL-6 in adult goats. Our findings suggest that IL-6 may play a key role in testicular development and function; however, further research is needed.

scholarly journals Regulation of Interleukin-6 (IL-6) Messenger RNA Expression in Rat Leydig and Sertoli Cells In Vitro

1997 ◽  
Vol 73 (4) ◽  
pp. 511-519
Author(s):  
Masato FUJISAWA ◽  
Yoshihiro OKUDA ◽  
Hiroshi OKADA ◽  
Soichi ARAKAWA ◽  
Sadao KAMIDONO
Keyword(s):  
Sertoli Cells ◽  
Interleukin 6 ◽  
Messenger Rna ◽  
Rna Expression

scholarly journals Transcriptional Regulation of Sertoli Cell Immediate Early Genes by Interleukin-6 and Interferon-γ Is Mediated through Phosphorylation of STAT-3 and STAT-1 Proteins*

Endocrinology ◽  
1997 ◽  
Vol 138 (7) ◽  
pp. 2740-2746 ◽  
Author(s):  
Shirzad Jenab ◽  
Patricia L. Morris
Keyword(s):  
Gene Expression ◽  
Tyrosine Phosphorylation ◽  
Sertoli Cells ◽  
Interleukin 6 ◽  
Immediate Early Genes ◽  
Interferon Γ ◽  
Immediate Early ◽  
Mrna Levels ◽  
Early Genes ◽  
Ifn Γ

Abstract The immediate early genes are regulated by a variety of extracellular signals, including pleiotropic cytokines. The effects of the testicular cytokines, interleukin-6 (IL-6) and interferon-γ (IFN-γ), on signal transducers and activators of transcription 3 and 1 (STAT-3 and STAT-1) and on c-fos gene expression in primary Sertoli cells are suggestive of their roles in differential function. Using the tyrosine phosphorylation inhibitor, genistein, and electrophoretic mobility shift assay, we show that IL-6 and IFN-γ induce nuclear factor STAT-3 and STAT-1 DNA-binding activity to the sis-inducible element of c-fos in a genistein-dependent pathway. Quantitative solution hybridization, Northern blot, and nuclear run-on analysis show that differential induction of c-fos, junB, and c-myc messenger RNA (mRNA) by these cytokines occur at transcriptional levels. IL-6 stimulates c-fos mRNA levels by 6-fold while increasing junB levels by 2-fold. IFN-γ increases c-fos message 2-fold, but has no effect on junB mRNA levels. Furthermore, genistein treatment blocks the induction of c-fos and junB gene expression, demonstrating that tyrosine phosphorylation of STAT proteins is involved in the cytokine regulation of the Sertoli immediate early genes. H7, a serine/threonine phosphorylation inhibitor, also blocks c-fos gene induction by IL-6 and IFN-γ, but does not affect the DNA-binding activities of STAT-3 and STAT-1. Finally, IL-6 treatment of Sertoli cells (3–6 h) increases the amounts of activating protein-1 binding to activating protein-1 element and c-myc transcription.

Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

1978 ◽  
Vol 36 (2) ◽  
pp. 340-341
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis Mcginley
Keyword(s):  
Organ Culture ◽  
Tight Junctions ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Barrier Function ◽  
Cell Junction ◽  
Intramembranous Particles ◽  
Junctional Complexes ◽  
Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

Annulate lamellae in the Sertoli cells of guinea pig testis after unilateral torsion of the spermatic cord

1984 ◽  
Vol 42 ◽  
pp. 196-197
Author(s):  
J. Chakraborty ◽  
A. P. Sinha Hikim ◽  
J. S. Jhunjhunwala
Keyword(s):  
Sertoli Cells ◽  
Guinea Pigs ◽  
Spermatic Cord ◽  
Present Report ◽  
Cell Types ◽  
Annulate Lamellae ◽  
Spermatogenic Cells ◽  
Electron Microscopic ◽  
Structural Details ◽  
First Time

Although the presence of annulate lamellae was noted in many cell types, including the rat spermatogenic cells, this structure was never reported in the Sertoli cells of any rodent species. The present report is based on a part of our project on the effect of torsion of the spermatic cord to the contralateral testis. This paper describes for the first time, the fine structural details of the annulate lamellae in the Sertoli cells of damaged testis from guinea pigs.One side of the spermatic cord of each of six Hartly strain adult guinea pigs was surgically twisted (540°) under pentobarbital anesthesia (1). Four months after induction of torsion, animals were sacrificed, testes were excised and processed for the light and electron microscopic investigations. In the damaged testis, the majority of seminiferous tubule contained a layer of Sertoli cells with occasional spermatogonia (Fig. 1). Nuclei of these Sertoli cells were highly pleomorphic and contained small chromatinic clumps adjacent to the inner aspect of the nuclear envelope (Fig. 2).

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