Biochemistry test of bacteria-3 (API20e and Enterotube II)

2022 ◽  
pp. 89-96
Author(s):  
Cangliang Shen ◽  
Yifan Zhang
Keyword(s):  
2015 ◽  
Vol 7 (3) ◽  
pp. 196-203 ◽  
Author(s):  
Chikara Yamashita ◽  
Hiroshi Shigeto ◽  
Norihisa Maeda ◽  
Takako Torii ◽  
Yasumasa Ohyagi ◽  
...  

Central pontine myelinolysis (CPM), which was originally considered to be the result of rapid correction of chronic hyponatremia, is not necessarily accompanied by hyponatremia or drastic changes in serum sodium level. Here, we report a case of an anorexic 55-year-old male with a history of pharyngo-laryngo-esophagogastrectomy, initially hospitalized with status epilepticus. Although his consciousness gradually recovered as we were controlling his convulsion, it deteriorated again with new onset of anisocoria, and magnetic resonance imaging (MRI) at this point revealed CPM. Rapid change of serum sodium or osmolarity, which is often associated with CPM, had not been apparent throughout his hospitalization. Instead, a review of the serum biochemistry test results showed that serum phosphate had drastically declined the day before the MRI first detected CPM. In this case, we suspect that hypophosphatemia induced by refeeding syndrome greatly contributed to the occurrence of CPM.


2011 ◽  
Vol 340 ◽  
pp. 280-286 ◽  
Author(s):  
Wen Yi Zhang ◽  
Fang Yuan Tan ◽  
Ting Ting Zhao ◽  
Li Qiao Lu ◽  
Ning Han

Two strains of T1, Z1 ammonibacterium were isolated from the biofilm of the BAF reactor. According to the morphologic observation and physiological biochemistry test, the strains T1, Z1 were preliminarily identified asProteusandMicrococcus respectively. The research of the strains T1, Z1 for ammoniation characteristics indicated that the suitable temperature was 30°C, the suitable pH value was 7.0 and the best inoculum quantity was 5%. The strain T1 was strictly aerobic bacteria and Z1 was facultative aerobic bacteria. In these operating parameters, two strains of T1, Z1 were good of the degradation of organic nitrogen and the growth rate of ammonia nitrogen could respectively reach up to 86.91% and 69.98%. The strains T1, Z1 had a certainly practical value for treating the waste water with the high concentration of organic nitrogen.


2020 ◽  
Vol 9 (1) ◽  
pp. 14
Author(s):  
Hongxia Gao ◽  
Yan Liu ◽  
Guoqing Wang

The purpose of this study is to explore the practical application effect of TBL innovative teaching method in the course of clinical biochemistry test technology. The research object is all undergraduate students in the laboratory major of 2018 medical school of our university. The research method is comparative experiment method, the teaching method of LBL teaching is set as control group, while the experimental group adopts TBL teaching method. By the final teaching evaluation and the data collected in the process, the influence of TBL teaching methods on the teaching effect of the course is compared and analyzed. The evaluation methods are combined with subjective and objective evaluation. The evaluation methods include questionnaire, student mutual evaluation, final test and other multiple ways. And the results of this study showed that the average score of the experimental group using TBL teaching method was significantly higher than that of the control group using traditional teaching method. In addition, through the process evaluation, it was found that the students in the experimental group were superior to the students in the control group in terms of learning interest, communication ability and learning methods. As a result, the application of TBL teaching method in the teaching of clinical biochemistry test obviously promotes the teaching quality and effect of this course.


2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Meng-yun Zhang ◽  
Hui-hua Chen ◽  
Jing Tian ◽  
Hui-juan Chen ◽  
Ling-ling Zhu ◽  
...  

Danggui Shaoyao San (DSS), a traditional Chinese medicinal prescription, was widely used to reinforce earth to activate collaterals in ancient times. Recently, many clinical studies found that DSS had a renoprotection. In this study, we evaluated the effect of DSS on unilateral ureteral obstruction- (UUO-) induced renal fibrosis in rats and investigated the mechanisms underlying the effect. Sprague Dawley (SD) rats were randomized to UUO or Sham operation. After 1 day, the rats that underwent UUO were randomized to treatment for four experimental groups (n=10 each group): Sham, UUO only, UUO+ benazepril (Bena), and UUO+DSS. After 4 weeks, we demonstrated that DSS significantly suppressed UUO-induced renal hypertrophy by gravimetric. In addition, DSS obviously prevented UUO-induced disorder in renal structure and renal function by HE and biochemistry test. We also found that DSS abrogated UUO-induced renal fibrosis by Masson’s staining and collagen volume fraction (CVF) analysis; this is consistent with the western blot analysis that showed DSS abrogated the UUO-induced enhanced TGF-β1 and weakened BMP-7. Compared with the UUO only group, rats treated with DSS exhibited significant increase in vascular density, followed by decrease in hypoxia and HIF-1α protein level through western blot and immunofluorescence analysis. Furthermore, we also determined proteins of autophagy and DSS enhanced autophagy to prevent the damage-induced by UUO. Taken together, our findings demonstrated that DSS had a renoprotection effect in ameliorating renal fibrosis possibly via attenuating tissue hypoxia and regulating autophagy.


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