Isolation and Identification of Ammonibacteria and Ammoniation Characteristic Analysis

2011 ◽  
Vol 340 ◽  
pp. 280-286 ◽  
Author(s):  
Wen Yi Zhang ◽  
Fang Yuan Tan ◽  
Ting Ting Zhao ◽  
Li Qiao Lu ◽  
Ning Han
Keyword(s):  
Organic Nitrogen ◽  
Ph Value ◽  
Ammonia Nitrogen ◽  
Aerobic Bacteria ◽  
Strictly Aerobic ◽  
High Concentration ◽  
Characteristic Analysis ◽  
Isolation And Identification ◽  
Suitable Temperature ◽  
Biochemistry Test

Two strains of T1, Z1 ammonibacterium were isolated from the biofilm of the BAF reactor. According to the morphologic observation and physiological biochemistry test, the strains T1, Z1 were preliminarily identified asProteusandMicrococcus respectively. The research of the strains T1, Z1 for ammoniation characteristics indicated that the suitable temperature was 30°C, the suitable pH value was 7.0 and the best inoculum quantity was 5%. The strain T1 was strictly aerobic bacteria and Z1 was facultative aerobic bacteria. In these operating parameters, two strains of T1, Z1 were good of the degradation of organic nitrogen and the growth rate of ammonia nitrogen could respectively reach up to 86.91% and 69.98%. The strains T1, Z1 had a certainly practical value for treating the waste water with the high concentration of organic nitrogen.

scholarly journals The Treatment of High Concentration Dyeing Wastewater with Pulsed Current Electrocoagulation

2016 ◽  
Vol 10 (5) ◽  
pp. 87 ◽  
Author(s):  
Jun Wang ◽  
Hong Cheng Tan ◽  
Yong Liang Zhang ◽  
Yong Zhang Pan
Keyword(s):  
Electrode Materials ◽  
Ph Value ◽  
Anaerobic Treatment ◽  
Ammonia Nitrogen ◽  
Pulsed Current ◽  
Dyeing Wastewater ◽  
High Concentration ◽  
Operational Conditions ◽  
Effluent Quality ◽  
Pre Treatment

<p>In this study, a small pulsed current electrocoagulation device was used to treat high concentration dyeing wastewater from a specific dyeing mill, and the effects of the electrode materials, reaction time, voltage, pH value, and aeration on the results of the treatment were examined. The results showed that under the following operational conditions: electrode materials were iron electrodes, time period was 15 min, voltage was 120 V, and initial pH was approximately 6, the removal rates of the COD, ammonia nitrogen, and color were 79.45%, 23.89%, and 87.50%, respectively. On this basis, a pulsed current electrocoagulation device, with a handling capacity of 0.5 m<sup>3</sup>/h, was used to conduct a pilot plant test for a period of one month. The results showed that the effluent quality (COD 1217.4 mg/L and NH<sub>4</sub><sup>+</sup>-N 358.2 mg/L on average) of the high-concentration dyeing wastewater, whose COD and NH<sub>4</sub><sup>+</sup>-N concentrations were 5328 mg/L, 595 mg/L, respectively after the treatment of a pulsed current electrocoagulation reactor, was superior to the effluent quality (COD 1400 mg/L and NH<sub>4</sub><sup>+</sup>-N 450 mg/L) of the mill’s actual pre-treatment system (flocculation-anaerobic treatment-acidification), and fully reached the influent requirements of the subsequent aerobic treatment. The results of this study showed that pulsed current electrocoagulation reactors may be effectively used for the pre-treatment of high concentration dyeing wastewater due to the observed advantages, such as good treatment effects, small investment, and economical space occupation.</p>

Inhibition factor of ammonification in stored urine with fecal contamination

2008 ◽  
Vol 58 (6) ◽  
pp. 1187-1192 ◽  
Author(s):  
S. Hotta ◽  
N. Funamizu
Keyword(s):  
Ph Value ◽  
Fecal Contamination ◽  
Ammonia Nitrogen ◽  
Urea Hydrolysis ◽  
Urea Solution ◽  
Storage Process ◽  
High Concentration ◽  
Inhibition Factor ◽  
Optimum Ph ◽  
Negative Effect

Inhibition factors of urea hydrolysis caused by fecal contamination as source of urease were investigated. The urea hydrolysis was described by initial ammonification rate (IAR). Three factors for the inhibition (pH, free ammonia nitrogen (FAN) and salinity) were investigated in this study. It was found that the optimum pH value for the urea hydrolysis was obtained at pH 7 (30°C). Similar results were obtained from pH 5 to pH 8 whereas pH 9 provided a little amount of negative effect on the hydrolysis. Significant effect of the FAN as inhibition factor on the urea hydrolysis was obtained in the urea solution: the hydrolysis was observed within 120 mg-N/l of the FAN level in artificial urea solution with fecal contamination. However in storage process of real urine the urea hydrolysis was observed until the FAN level increased up to 2,000 mg-N/l. Salinity obviously provided negative effect on the urea hydrolysis in stored urine with fecal contamination at 30°C. It was assumed that high concentration of the NaCl (150 g/l) was required to achieve 50% reduction of the IAR.

Research on Strengthening Decomposition of Organic Nitrogen by Ammonibacteria in the Faber Cymbidium Plant Floating Island

2011 ◽  
Vol 340 ◽  
pp. 429-435
Author(s):  
Wen Yi Zhang ◽  
Rong Chen ◽  
Ting Ting Zhao ◽  
Pei Cheng Fang ◽  
Suo Hua Wu
Keyword(s):  
Organic Nitrogen ◽  
High Efficiency ◽  
Removal Rate ◽  
Sewage Treatment ◽  
Decomposition Reaction ◽  
Ammonia Nitrogen ◽  
Order Kinetic ◽  
High Concentration ◽  
Floating Island ◽  
Degradation Reaction

Owing to the characteristics of the effluent with high concentration of ammonia nitrogen and low removal rate in the most artificial plants floating islands, two strains A1, A2 with high-efficiency ammonification screened from the laboratory were selected to use for enhancing decomposition capability of organic nitrogen in the plants floating island in order to improve nitrogen removal. The result showed that the decomposition rate of organic nitrogen by strains A1, A2 were 81.80% and 72.68% respectively, at the same time, decomposition equation was in line with zero order degradation reaction. Moreover, decomposition rates were VA1=0.8310mg/L•h and VA2=0.7619mg/L•h. The simulated ecosystem of sewage treatment was constructed by faber cymbidium (evergreen plant) plant floating island, and then the decomposition reaction of organic nitrogen met first-order kinetic equation in which k was 0.0752. If the strain was put into the previous simulated ecosystem, hydraulic retention time was shortened greatly and the degradation speed of ammonia nitrogen could be accelerated by cooperating action between amonifying bacteria and plant floating island. Furthermore, the ammonia nitrogen of the effluent could meet first discharge demand of integrated wastewater discharge standard (GB8978-1996) after 48h.

scholarly journals Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal
Keyword(s):  
Ph Value ◽  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Nano Materials ◽  
Elongation At Break ◽  
Stable Suspension ◽  
Average Size ◽  
Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

scholarly journals Dynamics of Fermentation Parameters and Bacterial Community in High-Moisture Alfalfa Silage with or without Lactic Acid Bacteria

2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Community ◽  
Ph Value ◽  
Ammoniacal Nitrogen ◽  
High Concentration ◽  
High Moisture ◽  
Acid Accumulation ◽  
Alfalfa Silage ◽  
Fermentation Parameters

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.

Separation of Heavy Metal Ions from the Solution Obtained by Leaching Low-Grade Pyrolusite with Pyrite and H2SO4

2013 ◽  
Vol 773 ◽  
pp. 283-288
Author(s):  
Xing Zou ◽  
Xiang Quan Chen ◽  
Hai Chao Xie ◽  
Xiao Dan Qiu
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Separation Efficiency ◽  
Ph Value ◽  
Sulfate Solution ◽  
Ion Concentration ◽  
Manganese Sulfate ◽  
Low Grade ◽  
High Concentration

The manganese sulfate solution leached from low-grade pyrolusite with pyrite and H2SO4 contains heavy metal ions of high concentration, influencing the quality of the final products of manganese compounds and causing manganese ions not to be electrolyzed. The present study was focused on the separation of Co, Ni and Zn ions from the leached solution with BaS. By controlling the pH value at 5.0-6.5, temperature at 50-60°C, reaction time at 15 min and mixing velocity at 78 rpm, the heavy metal ions could be separated effectively. Under the above optimized conditions, the ion concentration of Co, Ni, and Zn in the solution was reduced to 0.06 mg.L-1, 0.27mg.L-1 and 0.01mg.L-1, and the separation efficiency was 99.72%, 99.18% and 99.9% respectively. The obtained pure solution meets the demands of manganese electrowinning.

High-strength nitrogen removal of opto-electronic industrial wastewater in membrane bioreactor - a pilot study

2003 ◽  
Vol 48 (1) ◽  
pp. 191-198 ◽  
Author(s):  
T.K. Chen ◽  
C.H. Ni ◽  
J.N. Chen ◽  
J. Lin
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Organic Nitrogen ◽  
Membrane Bioreactor ◽  
High Strength ◽  
Experimental Period ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Biological Degradation ◽  
The Past

The membrane bioreactor (MBR) system has become more and more attractive in the field of wastewater treatment. It is particularly attractive in situations where long solids retention times are required, such as nitrifying bacteria, and physical retention critical to achieving more efficiency for biological degradation of pollutant. Although it is a new technology, the MBR process has been applied for industrial wastewater treatment for only the past decade. The opto-electronic industry, developed very fast over the past decade in the world, is high technology manufacturing. The treatment of the opto-electronic industrial wastewater containing a significant quantity of organic nitrogen compounds with a ratio over 95% in organic nitrogen (Org-N) to total nitrogen (T-N) is very difficult to meet the discharge limits. This research is mainly to discuss the treatment capacity of high-strength organic nitrogen wastewater, and to investigate the capabilities of the MBR process. A 5 m3/day capacity of MBR pilot plant consisted of anoxic, aerobic and membrane bioreactor was installed for evaluation. The operation was continued for 150 days. Over the whole experimental period, a satisfactory organic removal performance was achieved. The COD could be removed with an average of over 94.5%. For TOC and BOD5 items, the average removal efficiencies were 96.3 and 97.6%, respectively. The nitrification and denitrification was also successfully achieved. Furthermore, the effluent did not contain any suspended solids. Only a small concentration of ammonia nitrogen was found in the effluent. The stable effluent quality and satisfactory removal performance mentioned above were ensured by the efficient interception performance of the membrane device incorporated within the biological reactor. The MBR system shows promise as a means of treating very high organic nitrogen wastewater without dilution. The effluent of TKN, NOx-N and COD can fall below 20 mg/L, 30 mg/L and 50 mg/L.

scholarly journals Particulate malate oxidation in strictly aerobic bacteria

1970 ◽  
Vol 118 (2) ◽  
pp. 27P-28P
Author(s):  
M Jones ◽  
H K King
Keyword(s):  
Aerobic Bacteria ◽  
Strictly Aerobic ◽  
Malate Oxidation

scholarly journals Isolation and Identification of Heavy Metal Tolerant Bacteria from Tannery Effluents

2016 ◽  
Vol 29 (1) ◽  
pp. 23-26
Author(s):  
M Iqbal Hossain ◽  
M Nural Anwar
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Bacillus Coagulans ◽  
Bacillus Firmus ◽  
Chemical Parameters ◽  
High Concentration ◽  
Isolation And Identification ◽  
Tannery Effluents ◽  
Physical And Chemical ◽  
Metal Tolerant Bacteria

The aim and objective of the study was to isolate and characterize heavy metal tolerant microorganisms from tannery effluents. Six effluent samples were collected aseptically and their physical and chemical parameters were determined. A total of 40 bacterial colonies were isolated from these effluent samples. Among them, six bacterial isolates were characterized provisionally as Alcaligenes aquamarinus, Bacillus coagulans, Bacillus firmus, Enterobacter cloacae, Pseudomonas alcaligens and Pseudomonas mendocina based on morphological, cultural and biochemical characteristics. The survibality and tolerance to heavy metals (Cr and Cu) of these isolates were examined. All the isolates were found to grow at high concentration of CuSO4 (95ppm/ml) and varying degrees of chromium (K2Cr2O7). The highest tolerance was shown by Alcaligenes aquamarinus. These heavy metal tolerant organisms could be potential agents for bioremediation of heavy metals polluted environment.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 23-26

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