Hypothalamic Neuropeptide Gene Expression in Postmenopausal Women

Purpose To determine whether plasma estradiol (E2) levels are related to gene expression in estrogen receptor (ER)–positive breast cancers in postmenopausal women. Materials and Methods Genome-wide RNA profiles were obtained from pretreatment core-cut tumor biopsies from 104 postmenopausal patients with primary ER-positive breast cancer treated with neoadjuvant anastrozole. Pretreatment plasma E2 levels were determined by highly sensitive radioimmunoassay. Genes were identified for which expression was correlated with pretreatment plasma E2 levels. Validation was performed in an independent set of 73 ER-positive breast cancers. Results The expression of many known estrogen-responsive genes and gene sets was highly significantly associated with plasma E2 levels (eg, TFF1/pS2, GREB1, PDZK1 and PGR; P < .005). Plasma E2 explained 27% of the average expression of these four average estrogen-responsive genes (ie, AvERG; r = 0.51; P < .0001), and a standardized mean of plasma E2 levels and ER transcript levels explained 37% (r, 0.61). These observations were validated in an independent set of 73 ER-positive tumors. Exploratory analysis suggested that addition of the nuclear coregulators in a multivariable analysis with ER and E2 levels might additionally improve the relationship with the AvERG. Plasma E2 and the standardized mean of E2 and ER were both significantly correlated with 2-week Ki67, a surrogate marker of clinical outcome (r = −0.179; P = .05; and r = −0.389; P = .0005, respectively). Conclusion Plasma E2 levels are significantly associated with gene expression of ER-positive breast cancers and should be considered in future genomic studies of ER-positive breast cancer. The AvERG is a new experimental tool for the study of putative estrogenic stimuli of breast cancer.

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Profile of adipose tissue gene expression in premenopausal and postmenopausal women

Menopause The Journal of The North American Menopause Society ◽

10.1097/gme.0b013e31820641da ◽

2011 ◽

Vol 18 (6) ◽

pp. 675-684 ◽

Cited By ~ 8

Author(s):

Cecilia Gomez-Santos ◽

Juan J. Hernandez-Morante ◽

Javier Margareto ◽

Eider Larrarte ◽

Xavier Formiguera ◽

...

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Postmenopausal Women ◽

Adipose Tissue Gene Expression ◽

Tissue Gene Expression

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IL-6 gene expression in adipose tissue of postmenopausal women and its association with metabolic risk factors

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2014.08.008 ◽

2015 ◽

Vol 399 ◽

pp. 87-94 ◽

Cited By ~ 4

Author(s):

Sadashiv ◽

Sunita Tiwari ◽

Vani Gupta ◽

Bhola Nath Paul ◽

Sandeep Kumar ◽

...

Keyword(s):

Gene Expression ◽

Risk Factors ◽

Adipose Tissue ◽

Postmenopausal Women ◽

Metabolic Risk Factors ◽

Metabolic Risk

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Combined Influence of Hormone Therapy and Eccentric Exercise on Myostatin-Related Gene Expression in Postmenopausal Women

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000402098.05102.33 ◽

2011 ◽

Vol 43 (Suppl 1) ◽

pp. 754-755

Author(s):

Christina Dieli ◽

Tanya M. Spektor ◽

Judd C. Rice ◽

Fred R. Sattler ◽

E. Todd Schroeder

Keyword(s):

Gene Expression ◽

Hormone Therapy ◽

Postmenopausal Women ◽

Eccentric Exercise ◽

Related Gene

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TGFbeta-BMP signaling pathway gene expression in osteoporotic postmenopausal women by DNA microarrays

Bone ◽

10.1016/j.bone.2011.03.350 ◽

2011 ◽

Vol 48 ◽

pp. S159

Author(s):

T. Koromila ◽

Z. Dailiana ◽

S. Samara ◽

C. Chassanidis ◽

V. Aleporou - Marinou ◽

...

Keyword(s):

Gene Expression ◽

Postmenopausal Women ◽

Signaling Pathway ◽

Dna Microarrays ◽

Bmp Signaling ◽

Pathway Gene ◽

Signaling Pathway Gene

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PO-237 MSTN gene polymorphism influence on WBV-induced changes on body composition in Chinese postmenopausal women

Exercise Biochemistry Review ◽

10.14428/ebr.v1i5.10673 ◽

2018 ◽

Vol 1 (5) ◽

Author(s):

Xiaohong Chen ◽

Yang Hu

Keyword(s):

Gene Expression ◽

Body Composition ◽

Postmenopausal Women ◽

Reporter Gene ◽

Regulatory Mechanisms ◽

Whole Body ◽

Luciferase Reporter ◽

Recombinant Vector ◽

Significant Difference ◽

Composition Changes

Objective To explore the association between MSTN rs3791783 polymorphism and body composition response to 3-month whole body vibration (WBV) intervention in postmenopausal women and to probe into the molecular regulatory mechanisms behind it. Methods A total of 90 postmenopausal women participated in the study, and 30 of them served as non-trained control group, 60 of them trained on a vibration platform for 3 months. Body composition included FM% (percentage of fat mass) and LM% were assessed by dual-energy X-ray absorptiometry at the baseline and 3 months. The rs3791783 polymorphism was genotyped by PCR–restriction fragment length polymorphism, and association analysis with the body composition changes induced-by WBV training was performed. The regulatory mechanisms of the rs3791783 polymorphism were explored through the dual-luciferase reporter assay. Results In the WBV group, LM% significant increased through 3-month WBV training (60.87±5.35, 61.63±5.05, P<0.05), meanwhile, FM% significant decreased (35.65±5.47, 34.86±5.16, P<0.05). In the CON group, no significant difference within group was detected in LM% and FM%, and significant group by time interactions were found in LM% and FM% (P <0.05). The subjects with an AA allele had significantly greater increase in LM% (60.27±5.28, 61.42±4.93, P<0.01) and decreases in FM% (36.35±5.35, 35.18±4.99, P<0.01) than those with GA allele (61.97±5.72, 62.13±5.59, P>0.05; 34.38±5.90, 34.19±5.79, P>0.05). The relative luciferase activity of the reporter gene in recombinant vector carrying the A allele was 3.302±1.121, markedly higher than that in recombinant vector carrying the G allele (1.760±0.920, p<0.05). Conclusions Three-month WBV training is beneficial for body composition among postmenopausal women and the individual with AA genotype of the rs3791783 polymorphism were more sensitive to WBV-induced body composition changes. The A allele can improve reporter gene expression level, indicating the effects of rs3791783 on gene expression.

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Effect of High-Intensity Interval Training Versus Moderate-Intensity Aerobic Continuous Training on Galectin-3 Gene Expression in Postmenopausal Women: A Randomized Controlled Trial

Journal of Aging and Physical Activity ◽

10.1123/japa.2019-0213 ◽

2020 ◽

Vol 28 (6) ◽

pp. 987-995

Author(s):

Diana Keyhani ◽

Bakhtyar Tartibian ◽

Arezou Dabiri ◽

Ana Maria Botelho Teixeira

Keyword(s):

Gene Expression ◽

Physical Activity ◽

Postmenopausal Women ◽

Density Lipoprotein ◽

Interval Training ◽

Moderate Intensity ◽

High Intensity Interval Training ◽

Continuous Training ◽

Galectin 3 ◽

High Intensity Interval

Galectin-3 is a pro-inflammatory biomarker associated with the pathogenesis of heart failure (HF). Physical-activity reduces the risk of heart-failure by modification of inflammation and fibrosis. The purpose of this study was to compare the effects of 8 weeks of high-intensity interval training (HIIT) versus moderate-intensity aerobic continuous training on a predictive factor of HF in postmenopausal women. Thirty sedentary postmenopausal women were randomly assigned to three groups. The first group performed the HIIT program at 60%–90%, and the second group performed an exercise program at 50%–65% of HR reserve. The control group maintained their normal daily regular physical activity level. The gene expressions of galectin-3 and lipid profiles were measured at the baseline and the end of Week 8. The HIIT and moderate-intensity aerobic continuous training attenuated the gene expression of galectin-3, serum low-density lipoprotein, cholesterol, and triglyceride concentrations and enhanced high-density lipoprotein concentrations. These changes were considerably higher in the HIIT group. Our results show that HIIT is superior to moderate-intensity aerobic continuous training in improving the decrease in HF risk in postmenopausal women.

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