Osteoarticular vascular corrosion casting using industrial polyurethane for the 3D representation of the vascular tree on human knee

Preparation of replicas of the complex arrangement of blood vessels in various organs and tissues has been accomplished by infusing low viscosity resins into the vasculature. Subsequent removal of the surrounding tissue by maceration leaves a model of the intricate three-dimensional anatomy of the blood vessels of the tissue not obtainable by any other procedure. When applied with care, the vascular corrosion casting technique can reveal fine details of the microvasculature including endothelial nuclear orientation and distribution (Fig. 1), locations of arteriolar sphincters (Fig. 2), venous valve anatomy (Fig. 3), and vessel size, density, and branching patterns. Because casts faithfully replicate tissue vasculature, they can be used for quantitative measurements of that vasculature. The purpose of this report is to summarize and highlight some quantitative applications of vascular corrosion casting. In each example, casts were prepared by infusing Mercox, a methyl-methacrylate resin, and macerating the tissue with 20% KOH. Casts were either mounted for conventional scanning electron microscopy, or sliced for viewing with a confocal laser microscope.

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Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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Load-Bearing in the Ovine Medial Tibial Condyle: Effect of Meniscectomy

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1633027 ◽

1993 ◽

Vol 06 (02) ◽

pp. 100-104 ◽

Cited By ~ 8

Author(s):

D. M. Pickles ◽

C. R. Bellenger

Keyword(s):

Knee Joint ◽

Contact Area ◽

Mammalian Species ◽

Total Removal ◽

Load Bearing ◽

Human Knee ◽

Biochemical Effects ◽

Pressure Sensitive ◽

Medial Meniscectomy ◽

Tibial Condyle

SummaryTotal removal of a knee joint meniscus is followed by osteoarthritis in many mammalian species. Altered load-bearing has been observed in the human knee following meniscectomy but less is known about biochemical effects of meniscectomy in other species. Using pressure sensitive paper in sheep knee (stifle) joints it was found that, for comparable loads, the load-bearing area on the medial tibial condyle was significantly reduced following medial meniscectomy. Also, for loads of between 50 N and 500 N applied to the whole joint, the slope of the regression of contact area against load was much smaller. Following medial meniscectomy, the ability to increase contact area as load increased was markedly reduced.The load bearing area on the medial tibial condyle was reduced following meniscectomy.

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Experimentally Applied Mechanical Load to the Human Knee Systematically Affects the Morphology of Articular Cartilage

10.1055/s-0038-1639516 ◽

2018 ◽

Author(s):

Grischa Bratke ◽

Steffen Willwacher ◽

David Maintz ◽

Gert-Peter Brüggemann

Keyword(s):

Articular Cartilage ◽

Mechanical Load ◽

Human Knee

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Design of a Human Knee Reeducation Mechanism

Acta Universitatis Sapientiae Electrical and Mechanical Engineering ◽

10.2478/auseme-2019-0004 ◽

2019 ◽

Vol 11 (1) ◽

pp. 42-53

Author(s):

Allaoua Brahmia ◽

Ridha Kelaiaia

Keyword(s):

Lower Limb ◽

Mechanical Design ◽

Kinematic Chain ◽

Lower Limbs ◽

Kinematic Structure ◽

Robotic Device ◽

Human Knee ◽

Kinematic Study ◽

Rehabilitation Exercise ◽

New Machine

Abstract To establish an exercise in open muscular chain rehabilitation (OMC), it is necessary to choose the type of kinematic chain of the mechanical / biomechanical system that constitutes the lower limbs in interaction with the robotic device. Indeed, it’s accepted in biomechanics that a rehabilitation exercise in OMC of the lower limb is performed with a fixed hip and a free foot. Based on these findings, a kinematic structure of a new machine, named Reeduc-Knee, is proposed, and a mechanical design is carried out. The contribution of this work is not limited to the mechanical design of the Reeduc-Knee system. Indeed, to define the minimum parameterizing defining the configuration of the device relative to an absolute reference, a geometric and kinematic study is presented.

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New dosimetric approach for multidimensional dose evaluation in gamma knife radiosurgery

Journal of Neurosurgery ◽

10.3171/jns.2000.93.supplement_3.0239 ◽

2000 ◽

Vol 93 (supplement_3) ◽

pp. 239-242 ◽

Cited By ~ 4

Author(s):

R. Foroni ◽

G. Gambraini ◽

U. Danesi ◽

M. Mauri ◽

E. Pompilio ◽

...

Keyword(s):

Gamma Knife ◽

Metal Ion ◽

Light Transmission ◽

Xylenol Orange ◽

Ferrous Sulphate ◽

Gamma Knife Radiosurgery ◽

Absorbed Dose ◽

Content Type ◽

3D Representation ◽

Fine Print

✓ During the past two decades, the progress in computerized treatment planning systems has led to more accurate imaging and therapy by using the gamma knife, especially with the smallest collimators (4 mm). However, the ionization chambers that have been used to calibrate the gamma knife are not useful with the smallest collimators because the chambers are too big compared with the irradiated volume. Therefore, it is important to develop more suitable dosimeters. This study proposes a new dosimeter method. The FriXyGel method proposed here is based on a phantom dosimeter, an acquisition chain, and dedicated software. This dosimeter uses an agarose gel into which a ferrous sulphate solution (Fricke solution) and a metal ion indicator (xylenol orange) are incorporated. The absorbed dose is detected through measurements of visible light transmission, imaged by means of a charge-coupled device camera provided with a suitable optical filter. Gel layers are imaged before and after irradiation, and the differences in light absorption are related to the absorbed dose. By choosing convenient thickness of gel layers and by building up a phantom with different gel slices, it is possible to obtain a three-dimensional (3D) representation of the absorbed dose. The final 3D representation is reached after several mathematical processes have been applied to the images. The first step identifies and reduces all factors that could alter the original data, such as nonuniformity in illumination. Then, after calibration procedures, it is possible to obtain absorbed dose values and to discover their 3D representation. This goal has been reached by developing appropriate software that performs all the calculations necessary for spatial representation routines and prompt comparison with theoretical calculations.

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