Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro

The growth factor/receptor combination of hepatocyte growth factor (HGF)/c-met has been postulated to be critical for mesenchymal-to-epithelial conversion and tubule formation in the developing kidney. We therefore isolated and immortalized cells from embryonic kidneys of met -/- transgenic mice to determine whether these cells were epithelial and able to chemotax and form tubules in vitro. The cells were immortalized with retrovirus expressing human papillomavirus 16 (HPV 16) E6/E7 genes. Two rapidly dividing clones were isolated and found to express the epithelial cell markers cytokeratin, zonula occludens-1, and E-cadherin but not to express the fibroblast marker vimentin. The met -/- cells were able to chemotax in response to epidermal growth factor and transforming growth factor-alpha (TGF-alpha) and form tubules in vitro in response to TGF-alpha but not HGF. These experiments suggest that the HGF/c-met axis is not essential for epithelial cell development in the embryonic kidney and demonstrate that other growth factors are capable of supporting early tubulogenesis.

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An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

Odontology ◽

10.1007/s10266-021-00630-3 ◽

2021 ◽

Author(s):

Sarita Giri ◽

Ayuko Takada ◽

Durga Paudel ◽

Koki Yoshida ◽

Masae Furukawa ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Epithelial Cell ◽

Gingival Epithelial Cell ◽

Hydrogen Peroxide Treatment

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Epigallocatechin gallate protects the human lens epithelial cell survival against UVB irradiation through AIF/Endo G signaling pathways in vitro

Cutaneous and Ocular Toxicology ◽

10.1080/15569527.2021.1879112 ◽

2021 ◽

pp. 1-25

Author(s):

Qiuxin Wu ◽

Zhongen Li ◽

Xiuzhen Lu ◽

Jike Song ◽

Hui Wang ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Survival ◽

Signaling Pathways ◽

Epigallocatechin Gallate ◽

Lens Epithelial Cell ◽

Human Lens ◽

Uvb Irradiation ◽

Human Lens Epithelial Cell

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Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo

Pharmaceuticals ◽

10.3390/ph14080753 ◽

2021 ◽

Vol 14 (8) ◽

pp. 753

Author(s):

Anna Matysik-Woźniak ◽

Waldemar A. Turski ◽

Monika Turska ◽

Roman Paduch ◽

Mirosław Łańcut ◽

...

Keyword(s):

Epithelial Cell ◽

Corneal Epithelium ◽

Kynurenic Acid ◽

Pharmaceutical Products ◽

Corneal Epithelial Cell ◽

Eye Drops ◽

Conjunctival Epithelium ◽

Corneal Erosion

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds. The aim of the present study is to evaluate the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. Normal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. In rabbits, after corneal de-epithelization, eye drops containing 0.002% and 1% KYNA were applied five times a day until full recovery. KYNA decreased metabolism but did not affect the proliferation of the corneal epithelium. It decreased both the metabolism and proliferation of conjunctival epithelium. KYNA enhanced the migration of corneal but not conjunctival epithelial cells. KYNA reduced the secretion of IL-1β and IL-6 from the corneal epithelium, leaving IL-10 secretion unaffected. The release of all studied cytokines from the conjunctival epithelium exposed to KYNA was unchanged. KYNA at higher concentration accelerated the healing of the corneal epithelium. These favorable properties of KYNA suggest that KYNA containing topical pharmaceutical products can be used in the treatment of ocular surface diseases.

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Silk Film Stiffness Modulates Corneal Epithelial Cell Mechanosignaling

Macromolecular Chemistry and Physics ◽

10.1002/macp.202170013 ◽

2021 ◽

Vol 222 (7) ◽

pp. 2170013

Author(s):

Michael G. Sun ◽

Yuncin Luo ◽

Tao Teng ◽

Victor Guaiquil ◽

Qiang Zhou ◽

...

Keyword(s):

Epithelial Cell ◽

Corneal Epithelial Cell ◽

Corneal Epithelial ◽

Film Stiffness ◽

Silk Film

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Fabrication of Polycaprolactone/Nano Hydroxyapatite (PCL/nHA) 3D Scaffold with Enhanced In Vitro Cell Response via Design for Additive Manufacturing (DfAM)

Polymers ◽

10.3390/polym13091394 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1394

Author(s):

Yong Sang Cho ◽

So-Jung Gwak ◽

Young-Sam Cho

Keyword(s):

Mechanical Properties ◽

Cell Response ◽

Structural Characteristics ◽

Structure Design ◽

Compressive Modulus ◽

Control Group ◽

Design For Additive Manufacturing ◽

3D Scaffold ◽

3D Printed

In this study, we investigated the dual-pore kagome-structure design of a 3D-printed scaffold with enhanced in vitro cell response and compared the mechanical properties with 3D-printed scaffolds with conventional or offset patterns. The compressive modulus of the 3D-printed scaffold with the proposed design was found to resemble that of the 3D-printed scaffold with a conventional pattern at similar pore sizes despite higher porosity. Furthermore, the compressive modulus of the proposed scaffold surpassed that of the 3D-printed scaffold with conventional and offset patterns at similar porosities owing to the structural characteristics of the kagome structure. Regarding the in vitro cell response, cell adhesion, cell growth, and ALP concentration of the proposed scaffold for 14 days was superior to those of the control group scaffolds. Consequently, we found that the mechanical properties and in vitro cell response of the 3D-printed scaffold could be improved by kagome and dual-pore structures through DfAM. Moreover, we revealed that the dual-pore structure is effective for the in vitro cell response compared to the structures possessing conventional and offset patterns.

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The immune response against myelin basic protein in two strains of rat with different genetic capacity to develop experimental allergic encephalomyelitis.

Journal of Experimental Medicine ◽

10.1084/jem.141.1.72 ◽

1975 ◽

Vol 141 (1) ◽

pp. 72-81 ◽

Cited By ~ 61

Author(s):

D E McFarlin ◽

S C Hsu ◽

S B Slemenda ◽

F C Chou ◽

R F Kibler

Keyword(s):

Immune Response ◽

T Cell ◽

Experimental Allergic Encephalomyelitis ◽

Cell Response ◽

Basic Protein ◽

T Cell Response ◽

Skin Tests ◽

Allergic Encephalomyelitis ◽

Experimental Allergic

After challenge with guiena pig basic protein (GPBP) Lewis (Le) rats, which are homozygous for the immune response experimental allergic encephalomyelitis (Ir-EAE) gene, developed positive delayed skin tests against GPBP and the 43 residue encephalitogenic fragment (EF); in addition, Le rat lymph node cells (LNC) were stimulated and produced migration inhibitory factor (MIF) when incubated in vitro with these antigens. In contrast Brown Norway (BN) rats, which lack the Ir-EAE gene, did not develop delayed skin tests to EF and their LNC were not stimulated and did not produce MIF when incubated in vitro with EF. These observations indicate that the Ir-EAE gene controls a T-cell response against the EF. Le rats produced measurable anti-BP antibody by radioimmunoassay after primary challenge. Although no antibody was detectable in BN rats by radioimmunoassay, radioimmunoelectrophoresis indicated that a small amount of antibody was formed after primary immunization. After boosting intraperitoneally, both strains of rat exhibited a rise in anti-BP antibody; which was greater in Le rats. In both strains of rat the anti-BP antibody reacted with a portion of the molecule other than the EF. Since EF primarily evokes a T cell response, it is suggested that the EF portion of the BP molecule may contain a helper determinant in antibody production.

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Transforming growth factor-alpha enhances alveolar epithelial cell repair in a new in vitro model

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1994.267.6.l728 ◽

1994 ◽

Vol 267 (6) ◽

pp. L728-L738 ◽

Cited By ~ 37

Author(s):

F. Kheradmand ◽

H. G. Folkesson ◽

L. Shum ◽

R. Derynk ◽

R. Pytela ◽

...

Keyword(s):

Growth Factor ◽

Epithelial Cell ◽

Wound Repair ◽

Wound Closure ◽

Transforming Growth Factor ◽

Alveolar Epithelial Cell ◽

Alveolar Epithelial ◽

Factor Alpha ◽

Vitro Model

Alveolar epithelial type II cells are essential for regenerating an intact alveolar barrier after destruction of type I cells in vivo. The first objective of these experimental studies was to develop an in vitro model to quantify alveolar epithelial cell wound repair. The second objective was to investigate mechanisms of alveolar epithelial cell wound healing by studying the effects of serum and transforming growth factor-alpha (TGF-alpha) on wound closure. Primary cultures of rat alveolar type II cells were prepared by standard methods and grown to form confluent monolayers in 48 h. Then a wound was made by denuding an area (mean initial area of 2.1 +/- 0.6 mm2) of the monolayer. Re-epithelialization of the denuded area over time in the presence or absence of serum was measured using quantitative measurements from time-lapse video microscopy. The half time of wound healing was significantly enhanced in the presence of serum compared with serum-free conditions (2.4 +/- 0.2 vs. 17.4 +/- 0.8 h, P < 0.001). We then tested the hypothesis that TGF-alpha is an important growth factor for stimulating wound repair of alveolar epithelial cells. Exogenous addition of TGF-alpha in serum-free medium resulted in a significantly more rapid wound closure, and, furthermore, the addition of a monoclonal antibody to TGF-alpha in the presence of serum significantly decreased fourfold the rate of wound closure. Measurement of internuclear cell distance confirmed that both cell motility and cell spreading were responsible for closure of the wound. These data demonstrate that 1) the mechanisms of alveolar cell repair can be studied in vitro and that 2) TGF-alpha is a potent growth factor that enhances in vitro alveolar epithelial cell wound closure.

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