scholarly journals Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats

2015 ◽  
Vol 51 (1) ◽  
pp. 53-63 ◽  
Author(s):  
Mohammad Afiq Hamsi ◽  
Faizah Othman ◽  
Srijit Das ◽  
Yusof Kamisah ◽  
Zar Chi Thent ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Experimental Study ◽  
Coconut Oil ◽  
Inflammatory Biomarkers ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Sprague Dawley Rats

scholarly journals Effect of Virgin Coconut Oil Supplementation on Obese Rats’ Anthropometrical Parameters and Gut Bacteroidetes and Firmicutes Change Ratio

CORD ◽  
2016 ◽  
Vol 32 (1) ◽  
pp. 14
Author(s):  
Amadeus Driando Ahnan
Keyword(s):  
Gut Microbiota ◽  
Molecular Mechanisms ◽  
Coconut Oil ◽  
Fold Increase ◽  
Abdominal Circumference ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Anthropometric Parameters ◽  
Standard Curve ◽  
Sprague Dawley Rats

Previous clinical trials using virgin coconut oil (VCO) showed a potential anti-obesity effect, including for human, by reducing the waist circumference. However, there was not discovered any research that examined its anti-obesity effect in relation with gut microbiota parameters as one of current approaches of obesity parameters, for example Firmicutes and Bacteroidetes. This research was conducted to examine the effect of VCO supplementation on the change of Firmicutes and Bacteroidetes in comparison to the anthropometrical parameters. The experiment was done using 14 days obese-induced female Sprague-Dawley rats supplemented with 0.01% w/w and 0.02% w/w VCO for 28 days. The anthropometric data collection consisted of body weight (BW), body mass index (BMI), abdominal circumference (AC), and body length (BL). Ratio changes in –fold increase between Day 28 and Day 0 of Bacteroidetes and Firmicutes were retrieved by using relative quantification normalized against unit mass calculation, using the cycle threshold values from real-time polymerase chain reaction (RT-PCR) method with SYBR Green fluorescence and standard curve derived from tenfold 5 points dilution series of recombinant plasmid for efficiency calculation. VCO supplementation stimulated significant increase on anthropometric parameters on BW and AC (p<0.05), while insignificant changes were shown on Bacteroidetes and Firmicutes ratio with tendency of increasing and decreasing respectfully, with no significance. The result indicated that VCO supplementation in the given doses promoted pro-obesity effect on key anthropometric parameters, while showed potential anti-obesity effect on the gut microbiota parameter. Further researches were needed especially in determining the effective dose to promote the anti-obesity effect of VCO and revealing any possible cellular and molecular mechanisms.

scholarly journals Effectiveness Of Stepping Dose Ozonated Virgin Coconut Oil For Wound Healing In Autologous Full Thickness Skin Graft In Terms Of Fibroblast Proliferation And The Expression Of Vascular Endothelial Growth Factor In Sprague Dawley Rats

2021 ◽  
Vol 8 (1) ◽  
pp. 28-38
Author(s):  
Johan Rinto Even Napitupulu ◽  
Hardian Hardian ◽  
Najatullah Najatullah
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Skin Graft ◽  
Coconut Oil ◽  
Vascular Endothelial ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Sprague Dawley Rats ◽  
Endothelial Growth Factor

Abstract Background: Now, Skin grafts are one of the therapies of choice in the wound healing process. And this is still developing today. The process of formation fibroblas and the formation of vascular endothelial growth factor have an important role in healing skin graft wounds. Ozonated virgin coconut oil has an important role in wound healing. Objective: To prove the effect of ozonated virgin coconut oil in various doses in increasing number of fibroblas and expression of vascular endothelial growth factor of skin graft wounds. Methods: This study is an experimental study with "Randomized parallel study with controlled group design" on 40 Sprague Dawley rats which are performed an autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 and K2 = without ozonated VCO), (A1 and A2 = ozonated VCO 50,4 mg/ml), (B1 and B2 = ozonated VCO  103,2 mg/ml), (C1 and C2  = ozonated VCO 204  mg/ml). Evaluation of the amount of fibroblas was done by staining hematoxylin & eosin after the 6th day and the expression of vascular endothelial growth factor  was done by staining immunohistochemistry after the 12th  day after skin graft. Results: Statistical analysis of the amount of fibroblas after the 6th  day was found to be significantly different between the K1 vs A1 group (p = 0.029), K1 vs B1 (p = 0.004), K1 vs C1 (p = 0.000), on the 12th day was found significantly different the amount of fibroblast between K2 vs A2 ( p = 0,029 ), K2 vs B2 ( p = 0,010 ), K2 vs C2 ( p = 0,001 ). The expression of vascular endothelial growth factor on the 6th day was found a significant difference between the K1 vs A1 group (p = 0.024), K1 vs B1 (p = 0.005), K1 vs C1 (p = 0.001), on the 12th day was found significantly different the expression of vascular endothelial growth factor between K2 vs A2 ( p = 0,011 ), K2 vs B2 ( p = 0,036 ), K2 vs C2 ( p = 0,004 ) . Conclusion: Ozonated VCO can increase the amount of fibroblas and expression of vascular endothelial growth factor  in the wound healing process of autologous skin graft in Sprague Dawley rats. Keywords: Ozonated VCO, full thickness skin graft, fibroblas, VEGF.

VIRGIN COCONUT OIL MODULATES TCD4+ AND TCD8+ CELL PROFILE OF DOXORUBICIN-INDUCED IMMUNE-SUPPRESSED RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 37
Author(s):  
Jansen Silalahi ◽  
Rosidah Rosidah ◽  
Rosidah Rosidah ◽  
Yuandani Yuandani ◽  
Yuandani Yuandani ◽  
...  
Keyword(s):  
Coconut Oil ◽  
Control Group ◽  
Cancer Therapies ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Drug Of Choice ◽  
Sprague Dawley Rats ◽  
Group 3 ◽  
Group 2 ◽  
Cell Profile

 Objective: Doxorubicin (DOX) is a drug of choice in many cancer therapies. Virgin coconut oil (VCO) is one of nutraceutical which has many biology activities. This current study was carried out to investigate the VCO activity in modulating TCD4+, and TCD8+ cells profile toward rats which induced by DOX.Methods: A total of 15 Sprague-Dawley rats were divided into three groups consisting of five rats each as follows: Group 1, receiving oral saline 10 mL Kg BW (control group); Group 2, receiving oral saline 10 mL/kg BW; and Group 3, receiving VCO 5 mL/kg BW. Group 2 and 3 were administered with DOX intramuscularly at dose 4.67 mg/kg BW at day 1 and 4 to suppressed immune functions.Results: Treatment of VCO 5 mL/kg BW succeeded in reducing a side effect of DOX based on increasing the TCD4+ and TCD8+ blood level.Conclusion: The results reveal that VCO could increase the level of TCD4+ and TCD8+ in rats which induced by DOX.

scholarly journals EFFECTIVENESS OF OZONATED VIRGIN COCONUT OIL ON FULL THICKNESS SKIN DEFECT WOUND HEALING IN SPRAGUE DAWLEY Rats

2021 ◽  
Vol 8 (2) ◽  
pp. 220-228
Author(s):  
Herry Maha Putra Surbakti ◽  
Renny Yuniati ◽  
Djoko Handojo
Keyword(s):  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Full Thickness ◽  
Tgf Beta ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Sprague Dawley Rats ◽  
Significant Difference

AbstractIntroduction: Wounds are a breakdown of tissue integrity / continuity that can lead to infection. Virgin Coconut Oil is a processed coconut product made by processing fresh coconut flesh at low temperature and has a high nutritional content. Ozone therapy is an alternative therapy that has disinfectant properties and can induce strong oxidative stress. Methods: This study was conducted on 50 Sprague-Dawley rats that had injuries. We performed full thickness wounds and administered ozonated doses of VCO to mice. We looked at shrinkage of wounds and TGF Beta levels in mice. Assessment was carried out on day 7 and day 14 to assess the wound and TGF-beta immunohistochemically. Results: There was a significant difference in the wound shrinkage variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. We also found a significant difference in the TGF-beta variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. Conclusion: The administration of ozonated virgin coconut oil was effective in increasing the expression of TGF-? in the full thickness defect of Sprague Dawley rats and was effective in increasing the reduction in wound size in the full thickness defect of Sprague Dawley rats.

scholarly journals Increased superoxide levels in ganglia and sympathoexcitation are involved in sarafotoxin 6c-induced hypertension

2008 ◽  
Vol 295 (5) ◽  
pp. R1546-R1554 ◽  
Author(s):  
Melissa Li ◽  
Xiaoling Dai ◽  
Stephanie Watts ◽  
David Kreulen ◽  
Gregory Fink
Keyword(s):  
Blood Pressure ◽  
Sympathetic Innervation ◽  
Sympathetic Ganglia ◽  
Plasma Norepinephrine ◽  
Sprague Dawley ◽  
Surgical Ablation ◽  
Arterial Blood ◽  
Sprague Dawley Rats ◽  
Induced Hypertension ◽  
Hypertension Development

Endothelin (ET) type B receptors (ETBR) are expressed in multiple tissues and perform different functions depending on their location. ETBR mediate endothelium-dependent vasodilation, clearance of circulating ET, and diuretic effects; all of these should produce a fall in arterial blood pressure. However, we recently showed that chronic activation of ETBR in rats with the selective agonist sarafotoxin 6c (S6c) causes sustained hypertension. We have proposed that one mechanism of this effect is constriction of capacitance vessels. The current study was performed to determine whether S6c hypertension is caused by increased generation of reactive oxygen species (ROS) and/or activation of the sympathetic nervous system. The model used was continuous 5-day infusion of S6c into male Sprague-Dawley rats. No changes in superoxide anion levels in arteries and veins were found in hypertensive S6c-treated rats. However, superoxide levels were increased in sympathetic ganglia from S6c-treated rats. In addition, superoxide levels in ganglia increased progressively the longer the animals received S6c. Treatment with the antioxidant tempol impaired S6c-induced hypertension and decreased superoxide levels in ganglia. Acute ganglion blockade lowered blood pressure more in S6c-treated rats than in vehicle-treated rats. Although plasma norepinephrine levels were not increased in S6c hypertension, surgical ablation of the celiac ganglion plexus, which provides most of the sympathetic innervation to the splanchnic organs, significantly attenuated hypertension development. The results suggest that S6c-induced hypertension is partially mediated by sympathoexcitation to the splanchnic organs driven by increased oxidative stress in prevertebral sympathetic ganglia.

Sign in / Sign up

Export Citation Format

Share Document