scholarly journals Epigenome-wide DNA methylation in maternal blood and preterm birth (PTB)

2022 ◽  
Vol 226 (1) ◽  
pp. S9-S10
Author(s):  
Tracy A. Manuck ◽  
Kyle Roell ◽  
Lauren Eaves ◽  
Rebecca Fry
Keyword(s):  
Dna Methylation ◽  
Preterm Birth ◽  
Maternal Blood

scholarly journals Genome-wide DNA methylation can identify preterm birth-related genes in maternal blood

2020 ◽  
Author(s):  
Young-Ah You ◽  
Eun Jin Kwon ◽  
Han-Sung Hwang ◽  
Suk-Joo Choi ◽  
Sae Kyung Choi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Preterm Birth ◽  
Maternal Blood ◽  
Differential Methylation ◽  
Multiple Tests ◽  
Cpg Sites ◽  
Genome Wide ◽  
Increased Risk ◽  
Health And Disease ◽  
Whole Blood Cells

Abstract Background Preterm birth is associated with an increased risk of neonatal complications and death, as well as poor health and disease later in life. Epigenetics could contribute to the mechanism underlying preterm birth. Results Genome-wide DNA methylation in whole blood cells from ten women was assessed using Illumina Infinium HumanMethylation450 BeadChips array. We identified 6,755 differentially methylated CpG sites between term and preterm birth. Although no differential methylation of these CpGs were found in correcting for multiple tests, seven VTRNA2-1 CpGs in promotor region of island were detected in top different methylation. We performed pyrosequencing validation with blood samples from the pregnant women. The methylation levels of VTRNA2-1 were either low (hypomethylated, 0–12.2%) or high (hypermethylated, 32.6–50.8%). Hypermethylation of VTRNA2-1 was associated with an increased risk of preterm birth after adjusting for maternal age, delivered season, parity and count of white blood cell. The mRNA expression of VTRNA2-1 was 0.51-fold lower in PTB delivered women compared with women with term deliveries. Conclusion This study suggests that change of VTRNA2-1 methylation is related to PTB in maternal blood. Further elucidate to underlay mechanisms of preterm birth and affect to future systems biology studies to predict preterm birth.

scholarly journals Maternal blood and amnionic oxytocin receptor gene expression and serum oxytocin levels in preterm birth: a case control study

2020 ◽  
Author(s):  
KUMARI ANUKRITI ◽  
KIRAN GULERIA ◽  
VIPIN TYAGI ◽  
AMITA SUNEJA ◽  
BASU DEV BANERJEE
Keyword(s):  
Gene Expression ◽  
Preterm Birth ◽  
Quantitative Polymerase Chain Reaction ◽  
Receptor Gene ◽  
Active Phase ◽  
Maternal Blood ◽  
Oxytocin Receptor ◽  
Maternal Serum ◽  
Oxytocin Receptor Gene ◽  
Gene Expression Studies

BACKGROUND: The oxytocin (OXT)-oxytocin receptor (OXTR) system provides promising candidate gene for studies of genetic contributions to prematurity. OBJECTIVE: Quantification and comparison of oxytocin receptor (OXTR) gene expression and serum OXT levels in the blood and amnion of women delivering preterm and evaluation of the correlation between OXTR gene expression in blood and amnion with serum OXT levels in them. METHODS: 70 pregnant women in spontaneous labor delivering vaginally preterm i.e < 37 weeks and equal number of matched controls delivering spontaneously at term (37-42 weeks) were recruited. Maternal serum OXT levels taken in active stage of labor (i.e 4 cm cervical dilatation) were quantified by ELISA. Gene expression studies in the maternal blood and amnion were done by using real time quantitative polymerase chain reaction (RT-qPCR). RESULTS: The mean serum OXT level in PTL was 48.56 +- 6.97 pg/ml; significantly higher than in controls (43.00 +- 3.96 pg/ml), p<0.001. OXTR gene expression both in maternal blood (2.5 times) and amnion (3.5 times) were significantly higher in PTL. A significant positive correlation was observed between serum OXT levels and OXTR gene expression in amnion (r = -0.190, p = 0.025). CONCLUSIONS: The serum OXT levels and OXTR gene expression in amnion surge significantly in active phase of PTL. Thus, amnion probably links OXT-PTGs autocrine paracrine circuit to facilitate PTL. Future studies are needed to devise better OXTR receptor antagonists preferably acting on amnionic OXTRs to prevent PTL. KEYWORDS: Preterm birth, Preterm labor, Oxytocin, Oxytocin receptor, Placenta, Amnion

scholarly journals Hypermethylation of the VTRNA2-1 promoter in maternal blood is associated with preterm birth

2020 ◽  
Author(s):  
Young-Ah You ◽  
Eun Jin Kwon ◽  
Han-Sung Hwang ◽  
Suk-Joo Choi ◽  
Sae Kyung Choi ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Large Population ◽  
Underlying Mechanism ◽  
Maternal Blood ◽  
Blood Cell Count ◽  
Genome Wide ◽  
Increased Risk ◽  
Health And Disease ◽  
Whole Blood Cells ◽  
Dna Methylation Analysis

Abstract Background: Preterm birth is associated with an increased risk of neonatal complications and death, as well as poor health and disease later in life. Epigenetics could contribute to the mechanism underlying preterm birth. Results: Genome-wide DNA methylation analysis of whole blood cells from ten women was performed using an Illumina Infinium HumanMethylation450 BeadChips array. We identified 1,581 differentially methylated CpG sites in promotor regions between term and preterm birth. Although the differences were not significant after correcting for multiple tests, seven CpGs on the genomically imprinted VTRNA2-1 showed the largest differences (range: 26–39%). Pyrosequencing verification was performed with blood samples from pregnant women recruited additionally (n = 82). In total, 28 (34.1%) cases showed hypomethylation of the VTRNA2-1 promoter (< 13% methylation), while 54 cases (65.9%) showed a methylation level of 30–60%. Hypermethylation of VTRNA2-1 was associated with an increased risk of preterm birth after adjusting for maternal age, season of delivery, parity and white blood cell count. The mRNA expression of VTRNA2-1 was 0.51-fold lower in women with preterm deliveries (n = 20) compared with women with term deliveries (n = 20). Conclusions: Our results suggest that changes in VTRNA2-1 methylation in maternal blood are related to preterm birth. Further studies are needed to confirm the association of VTRNA2-1 methylation with preterm birth in a large population, and to elucidate the underlying mechanism.

scholarly journals Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

2019 ◽  
Vol 55 (83) ◽  
pp. 12451-12454 ◽  
Author(s):  
Suraj Pavagada ◽  
Robert B. Channon ◽  
Jason Y. H. Chang ◽  
Sung Hye Kim ◽  
David MacIntyre ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Minimally Invasive ◽  
Low Cost ◽  
Maternal Blood ◽  
Lateral Flow ◽  
Early Prediction ◽  
Circulating Micrornas ◽  
Lateral Flow Assays

Low-cost detection of miRNA biomarkers from maternal blood is achieved via a highly sequence-specific templated reaction on nitrocellulose paper strips to enable early prediction of preterm birth in a minimally invasive manner.

scholarly journals Differential DNA Methylation in Placenta Associated With Maternal Blood Pressure During Pregnancy

Hypertension ◽  
2020 ◽  
Vol 75 (4) ◽  
pp. 1117-1124
Author(s):  
Tsegaselassie Workalemahu ◽  
Marion Ouidir ◽  
Deepika Shrestha ◽  
Jing Wu ◽  
Katherine L. Grantz ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Dna Methylation ◽  
Maternal Blood ◽  
Maternal Blood Pressure

scholarly journals Longitudinal Changes of One-Carbon Metabolites and Amino Acid Concentrations during Pregnancy in the Women First Maternal Nutrition Trial

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Stephanie P Gilley ◽  
Nicholas E Weaver ◽  
Evan L Sticca ◽  
Purevsuren Jambal ◽  
Alexandra Palacios ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Amino Acid ◽  
Multiple Testing ◽  
Maternal Nutrition ◽  
Longitudinal Research ◽  
Maternal Blood ◽  
Replication Cohort ◽  
Multiple Testing Correction ◽  
Group A ◽  
Study Sites

ABSTRACT Background Maternal dietary restriction and supplementation of one-carbon (1C) metabolites can impact offspring growth and DNA methylation. However, longitudinal research of 1C metabolite and amino acid (AA) concentrations over the reproductive cycle of human pregnancy is limited. Objective To investigate longitudinal 1C metabolite and AA concentrations prior to and during pregnancy and the effects of a small-quantity lipid-based nutrition supplement (LNS) containing &gt;20 micronutrients and prepregnancy BMI (ppBMI). Methods This study was an ancillary study of the Women First Trial (NCT01883193, clinicaltrials.gov) focused on a subset of Guatemalan women (n = 134), 49% of whom entered pregnancy with a BMI ≥25 kg/m2. Ninety-five women received LNS during pregnancy (+LNS group), while the remainder did not (−LNS group). A subset of women from the Pakistan study site (n = 179) were used as a replication cohort, 124 of whom received LNS. Maternal blood was longitudinally collected on dried blood spot (DBS) cards at preconception, and at 12 and 34 wk gestation. A targeted metabolomics assay was performed on DBS samples at each time point using LC-MS/MS. Longitudinal analyses were performed using linear mixed modeling to investigate the influence of time, LNS, and ppBMI. Results Concentrations of 23 of 27 metabolites, including betaine, choline, and serine, changed from preconception across gestation after application of a Bonferroni multiple testing correction (P &lt; 0.00185). Sixteen of those metabolites showed similar changes in the replication cohort. Asymmetric and symmetric dimethylarginine were decreased by LNS in the participants from Guatemala. Only tyrosine was statistically associated with ppBMI at both study sites. Conclusions Time influenced most 1C metabolite and AA concentrations with a high degree of similarity between the 2 diverse study populations. These patterns were not significantly altered by LNS consumption or ppBMI. Future investigations will focus on 1C metabolite changes associated with infant outcomes, including DNA methylation. This trial was registered at clinicaltrials.gov as NCT01883193.

scholarly journals Whole-genome fetal and maternal DNA methylation analysis using MeDIP-NGS for the identification of differentially methylated regions

2016 ◽  
Vol 98 ◽  
Author(s):  
ANNA KERAVNOU ◽  
MARIOS IOANNIDES ◽  
KYRIAKOS TSANGARAS ◽  
CHARALAMBOS LOIZIDES ◽  
MICHAEL D. HADJIDANIEL ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Chorionic Villus ◽  
Maternal Blood ◽  
Maternal Plasma ◽  
Chorionic Villus Sampling ◽  
Whole Genome ◽  
Differentially Methylated Regions ◽  
Epigenetic Marker ◽  
Novel Approach ◽  
Targeted Enrichment

SummaryDNA methylation is an epigenetic marker that has been shown to vary significantly across different tissues. Taking advantage of the methylation differences between placenta-derived cell-free DNA and maternal blood, several groups employed different approaches for the discovery of fetal-specific biomarkers. The aim of this study was to analyse whole-genome fetal and maternal methylomes in order to identify and confirm the presence of differentially methylated regions (DMRs). We have initially utilized methylated DNA immunoprecipitation (MeDIP) and next-generation sequencing (NGS) to identify genome-wide DMRs between chorionic villus sampling (CVS) and female non-pregnant plasma (PL) and peripheral blood (WBF) samples. Next, using specific criteria, 331 fetal-specific DMRs were selected and confirmed in eight CVS, eight WBF and eight PL samples by combining MeDIP and in-solution targeted enrichment followed by NGS. Results showed higher enrichment in CVS samples as compared to both WBF and PL samples, confirming the distinct methylation levels between fetal and maternal DNA for the selected DMRs. We have successfully implemented a novel approach for the discovery and confirmation of a significant number of fetal-specific DMRs by combining for the first time MeDIP and in-solution targeted enrichment followed by NGS. The implementation of this double-enrichment approach is highly efficient and enables the detailed analysis of multiple DMRs by targeted NGS. Also, this is, to our knowledge, the first reported application of MeDIP on plasma samples, which leverages the implementation of our enrichment methodology in the detection of fetal abnormalities in maternal plasma.

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