scholarly journals Maternal blood and amnionic oxytocin receptor gene expression and serum oxytocin levels in preterm birth: a case control study

2020 ◽  
Author(s):  
KUMARI ANUKRITI ◽  
KIRAN GULERIA ◽  
VIPIN TYAGI ◽  
AMITA SUNEJA ◽  
BASU DEV BANERJEE
Keyword(s):  
Gene Expression ◽  
Preterm Birth ◽  
Quantitative Polymerase Chain Reaction ◽  
Receptor Gene ◽  
Active Phase ◽  
Maternal Blood ◽  
Oxytocin Receptor ◽  
Maternal Serum ◽  
Oxytocin Receptor Gene ◽  
Gene Expression Studies

BACKGROUND: The oxytocin (OXT)-oxytocin receptor (OXTR) system provides promising candidate gene for studies of genetic contributions to prematurity. OBJECTIVE: Quantification and comparison of oxytocin receptor (OXTR) gene expression and serum OXT levels in the blood and amnion of women delivering preterm and evaluation of the correlation between OXTR gene expression in blood and amnion with serum OXT levels in them. METHODS: 70 pregnant women in spontaneous labor delivering vaginally preterm i.e < 37 weeks and equal number of matched controls delivering spontaneously at term (37-42 weeks) were recruited. Maternal serum OXT levels taken in active stage of labor (i.e 4 cm cervical dilatation) were quantified by ELISA. Gene expression studies in the maternal blood and amnion were done by using real time quantitative polymerase chain reaction (RT-qPCR). RESULTS: The mean serum OXT level in PTL was 48.56 +- 6.97 pg/ml; significantly higher than in controls (43.00 +- 3.96 pg/ml), p<0.001. OXTR gene expression both in maternal blood (2.5 times) and amnion (3.5 times) were significantly higher in PTL. A significant positive correlation was observed between serum OXT levels and OXTR gene expression in amnion (r = -0.190, p = 0.025). CONCLUSIONS: The serum OXT levels and OXTR gene expression in amnion surge significantly in active phase of PTL. Thus, amnion probably links OXT-PTGs autocrine paracrine circuit to facilitate PTL. Future studies are needed to devise better OXTR receptor antagonists preferably acting on amnionic OXTRs to prevent PTL. KEYWORDS: Preterm birth, Preterm labor, Oxytocin, Oxytocin receptor, Placenta, Amnion

scholarly journals Polychlorinated biphenyl exposure alters oxytocin receptor gene expression and maternal behavior in rat model

2015 ◽  
Vol 3 (1) ◽  
pp. e979681 ◽  
Author(s):  
E Nicole Dover ◽  
David E Mankin ◽  
Howard C Cromwell ◽  
Vipaporn Phuntumart ◽  
Lee A Meserve
Keyword(s):  
Gene Expression ◽  
Rat Model ◽  
Maternal Behavior ◽  
Polychlorinated Biphenyl ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Receptor Gene Expression

scholarly journals 799: Does cholesterol exposure of myometrial cells affect oxytocin receptor gene expression and downstream signaling?

2020 ◽  
Vol 222 (1) ◽  
pp. S504-S505
Author(s):  
Ruchira Sharma ◽  
Prodyot Chatterjee ◽  
Xue Xiangying ◽  
Burton Rochelson ◽  
Christine Metz
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Myometrial Cells ◽  
Downstream Signaling ◽  
Receptor Gene Expression

scholarly journals Estrogen Regulates Transcription of the Ovine Oxytocin Receptor Gene through GC-Rich SP1 Promoter Elements

Endocrinology ◽  
2006 ◽  
Vol 147 (2) ◽  
pp. 899-911 ◽  
Author(s):  
JoAnn G. W. Fleming ◽  
Thomas E. Spencer ◽  
Stephen H. Safe ◽  
Fuller W. Bazer
Keyword(s):  
Gene Expression ◽  
Progesterone Receptor ◽  
Gene Transcription ◽  
Binding Sites ◽  
Promoter Activity ◽  
Receptor Gene ◽  
Prostaglandin F2α ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Esr1 Gene

Establishment of pregnancy in ruminants results from paracrine signaling by interferon τ (IFNT) from the conceptus to uterine endometrial luminal epithelia (LE) that prevents release of luteolytic prostaglandin F2α pulses. In cyclic and pregnant ewes, progesterone down-regulates progesterone receptor (PGR) gene expression in LE. In cyclic ewes, loss of PGR allows for increases in estrogen receptor α (ESR1) and then oxytocin receptor (OXTR) gene expression followed by oxytocin-induced prostaglandin F2α pulses. In pregnant ewes, IFNT inhibits transcription of the ESR1 gene, which presumably inhibits OXTR gene transcription. Alternatively, IFNT may directly inhibit OXTR gene transcription. The 5′ promoter/enhancer region of the ovine OXTR gene was cloned and found to contain predicted binding sites for activator protein 1, SP1, and PGR, but not for ESR1. Deletion analysis showed that the basal promoter activity was dependent on the region from −144 to −4 bp that contained only SP1 sites. IFNT did not affect activity of the OXTR promoter. In cells transfected with ESR1, E2, and ICI 182,780 increased promoter activity due to GC-rich SP1 binding sites at positions −104 and −64. Mutation analyses showed that the proximal SP1 sites mediated ESR1 action as well as basal activity of the promoter. In response to progesterone, progesterone receptor B also increased OXTR promoter activity. SP1 protein was constitutively expressed and abundant in the LE of the ovine uterus. These results support the hypothesis that the antiluteolytic effects of IFNT are mediated by direct inhibition or silencing of ESR1 gene transcription, thereby precluding ESR1/SP1 from stimulating OXTR gene transcription.

The role of the endometrial oxytocin receptor in determining the length of the sterile oestrous cycle and ensuring maintenance of luteal function in early pregnancy in ruminants

1994 ◽  
Vol 344 (1309) ◽  
pp. 291-304 ◽  
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Transmembrane Domain ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oestrous Cycle ◽  
High Rate ◽  
Endocrine Function ◽  
Oxytocin Receptor Gene ◽  
Receptor Gene Expression

The oxytocin receptor, a seven transmembrane domain, G protein-linked receptor molecule, plays a central role in determining the endocrine function of the ruminant uterine endometrium. During non- pregnant cycles the control of this molecule by circulating steroid hormones leads to regression of the corpora lutea. The kinetics of the mechanisms involved determine the time at which luteolysis occurs, and therefore the length of the oestrous cycle. In pregnancy, secretions of the trophoblast block endometrial oxytocin receptor gene expression and lead to luteal maintenance. An understanding of the molecular mechanisms involved in the steroidal control of oxytocin receptor gene expression will provide an explanation for the relative constancy of oestrous cycle lengths in non-pregnant animals. Unravelling the way in which trophoblast products block expression of the oxytocin receptor gene will lead to a better understanding of the reasons for the high rate of embryonic loss in domestic ruminants.

scholarly journals Oxytocin and Oxytocin Receptor Gene Expression in the Reproductive Tract of the Pregnant Cow: Rescue of Luteal Oxytocin Production at Term1

1995 ◽  
Vol 53 (3) ◽  
pp. 553-560 ◽  
Author(s):  
Richard Ivell ◽  
Werner Rust ◽  
Almuth Einspanier ◽  
Stefan Hartung ◽  
Michael Fields ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reproductive Tract ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Receptor Gene Expression

scholarly journals Common Oxytocin Receptor Gene Polymorphisms and the Risk for Preterm Birth

2013 ◽  
Vol 34 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Lorenz Kuessel ◽  
Christoph Grimm ◽  
Martin Knöfler ◽  
Peter Haslinger ◽  
Heinz Leipold ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Gene Polymorphisms ◽  
Haplotype Analysis ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Haplotype Combination ◽  
Increased Risk ◽  
Receptor Gene Polymorphisms

Oxytocin is crucially involved in the onset and maintenance of labor. We investigated the association between oxytocin receptor gene polymorphisms and preterm birth. The presence of four common oxytocin receptor gene polymorphisms (rs2254298, rs53576, rs2228485 and rs237911) was evaluated in one hundred women with preterm birth and one hundred healthy women using restriction fragment length polymorphism genotyping. No association was found between the presence of any individual oxytocin receptor gene polymorphism and preterm birth. In haplotype analysis, the haplotype combination of rs2254298 A allele, rs2228485 C allele and rs237911 G allele was found to be significantly associated with an increased risk of preterm birth (OR = 3.2 [CI 1.04–9.8],p= 0.043). In conclusion our findings suggest that a combination of three oxytocin receptor gene polymorphisms is associated with an increased risk for preterm birth. We propose further studies investigating the role of oxytocin receptor gene polymorphisms and preterm birth.

Estrogen increases renal oxytocin receptor gene expression.

Endocrinology ◽  
1995 ◽  
Vol 136 (4) ◽  
pp. 1801-1804 ◽  
Author(s):  
N L Ostrowski ◽  
W S Young ◽  
S J Lolait
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Receptor Gene Expression
Sign in / Sign up

Export Citation Format

Share Document