Effect of biomass concentration on the productivity of Tetraselmis suecica in a pilot-scale tubular photobioreactor using natural sunlight

2014 ◽  
Vol 4 ◽  
pp. 12-18 ◽  
Author(s):  
Michiel H.A. Michels ◽  
Petronella M. Slegers ◽  
Marian H. Vermuë ◽  
René H. Wijffels
Keyword(s):  
Biomass Concentration ◽  
Pilot Scale ◽  
Tetraselmis Suecica ◽  
Tubular Photobioreactor ◽  
Natural Sunlight

scholarly journals Green technologies for air pollution treatment by microalgae in tubular photobioreactor

2021 ◽  
Vol 926 (1) ◽  
pp. 012098
Author(s):  
T T Minh ◽  
N H Tien ◽  
L V Giang ◽  
H H Loc ◽  
V T D Hien ◽  
...  
Keyword(s):  
Air Pollution ◽  
Biomass Concentration ◽  
Absorption Efficiency ◽  
Green Technologies ◽  
Tubular Photobioreactor ◽  
Technology System ◽  
Study Approach ◽  
Bicarbonate Ions ◽  
Stable Growth ◽  
The Impact

Abstract Air pollution in general and motorcycle exhaust, in particular, is a big problem attracting a lot of attention from people and researchers worldwide because of the significant impacts it has on humans and the environment. The issue of air pollution is growing, and the impact is more evident than ever. Carbon dioxide represents a series of problems that we face daily but have not yet been effectively solved. Currently, microalgae are known to photosynthesize and use free CO2, bicarbonate ions as a source of nutrients to grow. Microalgae are developed under appropriate environmental conditions, which will bring admirable CO2 treatment efficiency and obtain biomass for other applications. The study approach was an inexpensive and natural air purification solution by microalgae, which is designed as a tubular photobioreactor. The study was conducted by evaluating the ability of Chlorella Vulgaris to grow and absorb CO2 emissions in the newly established system with exhaust gas supplied from a mini motorcycle engine. The results showed that microalgae grew stably in the tubular photobioreactor system with a biomass concentration of 6×106 cells/ml after 42 days of the experiment. Simultaneously with the stable growth of microalgae, the CO2 emission concentration was reduced with 26.59% absorption efficiency after 11 days of the experiment. Finally, establishing the tubular photobioreactor technology system has yielded impressive initial results in cultivating stable growing microalgae combined with CO2 emission treatment.

scholarly journals Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

2015 ◽  
Vol 52 (2) ◽  
pp. 59-70 ◽  
Author(s):  
J. Vanags ◽  
L. Kunga ◽  
K. Dubencovs ◽  
V. Galvanauskas ◽  
O. Grīgs
Keyword(s):  
Light Intensity ◽  
Shake Flask ◽  
Scale Up ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Laboratory Scale ◽  
Stirred Tank ◽  
Light Limitation ◽  
Maximum Biomass

Abstract Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).

Some observations on the effects of accumulated benthic sludge on the behaviour of waste stabilisation ponds

2005 ◽  
Vol 51 (12) ◽  
pp. 217-226
Author(s):  
C.J. Banks ◽  
S. Heaven ◽  
E.A. Zotova
Keyword(s):  
Light Intensity ◽  
Dissolved Oxygen ◽  
Water Column ◽  
Suspended Solids ◽  
Biomass Concentration ◽  
Pilot Scale ◽  
Controlling Factor ◽  
Waste Stabilisation Ponds ◽  
Nutrient Levels ◽  
Complex Mechanisms

The effect of accumulated bottom sludge on water column characteristics was studied in two pilot-scale ponds. Parameters measured were ammonia, nitrate, phosphate, COD, suspended solids, dissolved oxygen (DO), temperature and light intensity. The de-sludged pond showed a stronger correlation between DO, light intensity, nutrients and suspended solids with the controlling factor being availability of nitrogen. This was less apparent in the pond with sludge where nutrient levels were higher and more complex mechanisms controlled biomass concentration. Water column characteristics in the two ponds converged rapidly in 7–10 weeks, however, due to accumulation of fresh sludge.

Treatment of winery wastewaters in a membrane submerged bioreactor

2007 ◽  
Vol 56 (2) ◽  
pp. 63-69 ◽  
Author(s):  
P. Artiga ◽  
M. Carballa ◽  
J.M. Garrido ◽  
R. Méndez
Keyword(s):  
Tap Water ◽  
Oxygen Demand ◽  
Biomass Concentration ◽  
Pilot Scale ◽  
White Wine ◽  
Hollow Fibre ◽  
Organic Loading Rate ◽  
Wine Production ◽  
Solids Concentration ◽  
Start Up

Wine production is seasonal, and thus the wastewater flow and its chemical oxygen demand (COD) concentrations greatly vary during the vintage and non-vintage periods, as well as being dependant on the winemaking technologies used, e.g. red, white or special wines production. Due to this seasonal high variability in terms of organic matter load, the use of membrane biological reactors (MBR) could be suitable for the treatment of such wastewaters. MBR offers several benefits, such as rapid start up, good effluent quality, low footprint area, absence of voluminous secondary settler and its operation is not affected by the settling properties of the sludge. A pilot scale hollow fibre MBR system of 220 L was fed by adequately diluting white wine with tap water, simulating wastewaters generated in wineries. The COD in the influent ranged between 1,000 and 4,000 mg/L. In less than 10 days after the start up, the system showed a good COD removal efficiency. The COD elimination percentage was always higher than 97% regardless of the organic loading rate (OLR) applied (0.5–2.2 kg COD/m3 d), with COD concentrations in the effluent ranging between 20 and 100 mg/L. Although the biomass concentration in the reactor increased from 0.5 to 8.6 g VSS/L, the suspended solids concentration in the effluent was negligible. Apparent biomass yield was estimated in 0.14 g VSS/g COD.

scholarly journals Pilot-scale production of poly-β-hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor

2018 ◽  
Vol 34 ◽  
pp. 116-125 ◽  
Author(s):  
Clemens Troschl ◽  
Katharina Meixner ◽  
Ines Fritz ◽  
Klaus Leitner ◽  
Alejandra Palacios Romero ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Scale Production ◽  
Tubular Photobioreactor ◽  
Pilot Scale Production

Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

RSC Advances ◽  
2015 ◽  
Vol 5 (47) ◽  
pp. 37485-37494 ◽  
Author(s):  
Jitendra Kumar Saini ◽  
Ruchi Agrawal ◽  
Alok Satlewal ◽  
Reetu Saini ◽  
Ravi Gupta ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Biomass Concentration ◽  
Pilot Scale ◽  
Process Time ◽  
High Gravity ◽  
Thermotolerant Yeast ◽  
Second Generation Bioethanol ◽  
P Application ◽  
Pretreated Wheat Straw ◽  
High Biomass Concentration

Application of thermotolerant yeast Kluyveromyces marxianus DBTIOC-35 in SSF decreases overall process time, and increases productivity and yield by allowing elimination of presaccharification step and use of high biomass concentration, respectively.

Modelling of long-term simultaneous nitrification and denitrification (SNDN) performance of a pilot scale membrane bioreactor

2008 ◽  
Vol 57 (11) ◽  
pp. 1825-1833 ◽  
Author(s):  
M. Sarioglu ◽  
G. Insel ◽  
N. Artan ◽  
D. Orhon
Keyword(s):  
Nitrogen Removal ◽  
Dynamic Modelling ◽  
Biomass Concentration ◽  
Domestic Wastewater ◽  
Pilot Scale ◽  
Bulk Liquid ◽  
Endogenous Respiration ◽  
Total Biomass ◽  
Adopted Model ◽  
The Impact

Nutrient removal capability of the MBR process has attracted more attention than organics removal in the past few years. Apart from the conventional schemes for nitrogen removal in MBR process, simultaneous nitrification-denitrification (SNDN) requires the most attention for further research. In order to fully understand the fundemantals and mechanism of SNDN in MBRs, a pilot plant was set up. A mathematical model was adopted for investigation and calibration against the observed values. This paper reports a study focusing on evaluating major mechanisms that govern nitrogen removal from domestic wastewater in membrane bioreactors. Two items need to be emphasized in this evaluation: (i) an MBR is basically regarded as an activated sludge process–a suspended growth bioreactor with total biomass recycle and substantially higher biomass concentration; (ii) in this context an AS model, namely ASM1R modified for endogenous respiration, is used for dynamic modelling and calibration of experimental results. The impact of diffusion through biomass which obviously exerts a significant effect on system performance and denitrification is evaluated with success using the adopted model by means of switch functions that regulate nitrification-denitrification with respect to dissolved oxygen concentration in the bulk liquid.

scholarly journals Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
A. Barros ◽  
H. Pereira ◽  
J. Campos ◽  
A. Marques ◽  
J. Varela ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Biomass Concentration ◽  
Growth Conditions ◽  
Pilot Scale ◽  
Industrial Scale ◽  
Scale Production ◽  
Two Stage ◽  
Chlorophyll Contents ◽  
Large Scale Production

Abstract Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed – heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic microalgal production unit (2nd stage). A preliminary pilot-scale trial revealed that C. vulgaris cells grown heterotrophically adapted readily to outdoor autotrophic growth conditions (1-m3 photobioreactors) without any measurable difference as compared to conventional autotrophic inocula. Biomass concentration of 174.5 g L−1, the highest value ever reported for this microalga, was achieved in a 5-L fermenter during scale-up using the heterotrophic route. Inocula grown in 0.2- and 5-m3 industrial fermenters with mean productivity of 27.54 ± 5.07 and 31.86 ± 2.87 g L−1 d−1, respectively, were later used to seed several outdoor 100-m3 tubular photobioreactors. Overall, all photobioreactor cultures seeded from the heterotrophic route reached standard protein and chlorophyll contents of 52.18 ± 1.30% of DW and 23.98 ± 1.57 mg g−1 DW, respectively. In addition to providing reproducible, high-quality inocula, this two-stage approach led to a 5-fold and 12-fold decrease in scale-up time and occupancy area used for industrial scale-up, respectively.

scholarly journals The Cultivation of Lipid-Rich Microalgae Biomass as Anaerobic Digestate Valorization Technology—A Pilot-Scale Study

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 517 ◽  
Author(s):  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Marta Kisielewska ◽  
Joanna Kazimierowicz ◽  
Magda Dudek ◽  
...  
Keyword(s):  
Culture Medium ◽  
Biomass Concentration ◽  
Pilot Scale ◽  
Dairy Wastewater ◽  
Small Scale ◽  
Treatment Efficiency ◽  
Average Growth Rate ◽  
Average Growth ◽  
Bio Oil ◽  
Microalgae Biomass

The aim of the study was to determine the use of digestate from anaerobic digestion of dairy wastewater as a culture medium for microalgae to obtain bio-oil. The experiments were conducted at a small scale in a closed raceway pond. The efficiency of the microalgae biomass production, the digestate treatment efficiency as well as the content and properties of the bio-oil obtained from the microalgal cells were analyzed. The produced biomass concentration was about 3000 ± 10.5 mg dry biomass/L, with an average growth rate of 160 ± 6.6 mgdm/L·d. The efficiency of organic compound and nutrient removal was above 90%. The bio-oil content in the biomass was about 20%. Based on the results of the study, a concept for technical-scale technology was developed.

Life cycle assessment of the production of bioactive compounds from Tetraselmis suecica at pilot scale

2014 ◽  
Vol 64 ◽  
pp. 323-331 ◽  
Author(s):  
Paula Pérez-López ◽  
Sara González-García ◽  
R. Gabriela Ulloa ◽  
Jorge Sineiro ◽  
Gumersindo Feijoo ◽  
...  
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Bioactive Compounds ◽  
Pilot Scale ◽  
Tetraselmis Suecica
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