Effect of phytohormones from different classes on gene expression of Chlorella sorokiniana under nitrogen limitation for enhanced biomass and lipid production

2019 ◽  
Vol 40 ◽  
pp. 101518 ◽  
Author(s):  
Abhishek Guldhe ◽  
Nirmal Renuka ◽  
Poonam Singh ◽  
Faizal Bux
Keyword(s):  
Gene Expression ◽  
Nitrogen Limitation ◽  
Lipid Production ◽  
Chlorella Sorokiniana

scholarly journals The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 463 ◽  
Author(s):  
Ali Nawaz Kumbhar ◽  
Meilin He ◽  
Abdul Razzaque Rajper ◽  
Khalil Ahmed Memon ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Lipid Content ◽  
Fossil Fuels ◽  
Fossil Fuel ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Biofuel Production ◽  
Promising Strategy ◽  
Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

Accelerating lipid production in freshwater alga Chlorella sorokiniana SDEC-18 by seawater and ultrasound during the stationary phase

2020 ◽  
Vol 161 ◽  
pp. 448-456
Author(s):  
Zhen Xie ◽  
Haiyan Pei ◽  
Lijie Zhang ◽  
Zhigang Yang ◽  
Changliang Nie ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Lipid Production ◽  
Chlorella Sorokiniana ◽  
Freshwater Alga

scholarly journals Global Regulation of Gene Expression and Cell Differentiation in Caulobacter crescentus in Response to Nutrient Availability

2009 ◽  
Vol 192 (3) ◽  
pp. 819-833 ◽  
Author(s):  
Jennifer C. England ◽  
Barrett S. Perchuk ◽  
Michael T. Laub ◽  
James W. Gober
Keyword(s):  
Gene Expression ◽  
Nitrogen Limitation ◽  
Caulobacter Crescentus ◽  
Regulation Of Gene Expression ◽  
Fixed Time ◽  
Natural Environments ◽  
Swarmer Cell ◽  
Regulatory Strategies ◽  
Time Period ◽  
Regulated Gene Expression

ABSTRACT In a developmental strategy designed to efficiently exploit and colonize sparse oligotrophic environments, Caulobacter crescentus cells divide asymmetrically, yielding a motile swarmer cell and a sessile stalked cell. After a relatively fixed time period under typical culture conditions, the swarmer cell differentiates into a replicative stalked cell. Since differentiation into the stalked cell type is irreversible, it is likely that environmental factors such as the availability of essential nutrients would influence the timing of the decision to abandon motility and adopt a sessile lifestyle. We measured two different parameters in nutrient-limited chemostat cultures, biomass concentration and the ratio of nonstalked to stalked cells, over a range of flow rates and found that nitrogen limitation significantly extended the swarmer cell life span. The transcriptional profiling experiments described here generate the first comprehensive picture of the global regulatory strategies used by an oligotroph when confronted with an environment where key macronutrients are sparse. The pattern of regulated gene expression in nitrogen- and carbon-limited cells shares some features in common with most copiotrophic organisms, but critical differences suggest that Caulobacter, and perhaps other oligotrophs, have evolved regulatory strategies to deal distinctly with their natural environments. We hypothesize that nitrogen limitation extends the swarmer cell lifetime by delaying the onset of a sequence of differentiation events, which when initiated by the correct combination of external environmental cues, sets the swarmer cell on a path to differentiate into a stalked cell within a fixed time period.

scholarly journals A Mathematical Model of Neutral Lipid Content in terms of Initial Nitrogen Concentration and Validation in Coelastrum sp. HA-1 and Application in Chlorella sorokiniana

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Zhenhua Yang ◽  
Yue Zhao ◽  
Zhiyong Liu ◽  
Chenfeng Liu ◽  
Zhipeng Hu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Nitrogen Concentration ◽  
Lipid Production ◽  
Lipid Synthesis ◽  
Quantitative Relationship ◽  
Chlorella Sorokiniana ◽  
High Lipid ◽  
Nitrogen Concentrations

Microalgae are considered to be a potential major biomass feedstock for biofuel due to their high lipid content. However, no correlation equations as a function of initial nitrogen concentration for lipid accumulation have been developed for simplicity to predict lipid production and optimize the lipid production process. In this study, a lipid accumulation model was developed with simple parameters based on the assumption protein synthesis shift to lipid synthesis by a linear function of nitrogen quota. The model predictions fitted well for the growth, lipid content, and nitrogen consumption of Coelastrum sp. HA-1 under various initial nitrogen concentrations. Then the model was applied successfully in Chlorella sorokiniana to predict the lipid content with different light intensities. The quantitative relationship between initial nitrogen concentrations and the final lipid content with sensitivity analysis of the model were also discussed. Based on the model results, the conversion efficiency from protein synthesis to lipid synthesis is higher and higher in microalgae metabolism process as nitrogen decreases; however, the carbohydrate composition content remains basically unchanged neither in HA-1 nor in C. sorokiniana.

Cultivation of an indigenous Chlorella sorokiniana with phytohormones for biomass and lipid production under N-limitation

2017 ◽  
Vol 23 ◽  
pp. 178-185 ◽  
Author(s):  
A. Giridhar Babu ◽  
Xiaoge Wu ◽  
Akhil N. Kabra ◽  
Dong-Pyo Kim
Keyword(s):  
Lipid Production ◽  
Chlorella Sorokiniana ◽  
N Limitation

Study of the toxicity of ZnO nanoparticles to Chlorella sorokiniana under the influence of phosphate: spectroscopic quantification, photosynthetic efficiency and gene expression analysis

2020 ◽  
Vol 7 (5) ◽  
pp. 1431-1443
Author(s):  
Hong Zhang ◽  
Zhu Chen ◽  
Qing Huang
Keyword(s):  
Gene Expression ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Aquatic Environment ◽  
Zinc Oxide Nanoparticles ◽  
Photosynthetic Efficiency ◽  
Gene Expression Analysis ◽  
Chlorella Sorokiniana ◽  
Oxide Nanoparticles ◽  
Zno Nps

Zinc oxide nanoparticles (ZnO NPs) are one of the most abundantly applied nanomaterials in nanotechnology-based industries, and recent research continues to highlight their transformations and potential eco-toxicity especially to aquatic environment.

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