Study of the toxicity of ZnO nanoparticles to Chlorella sorokiniana under the influence of phosphate: spectroscopic quantification, photosynthetic efficiency and gene expression analysis

2020 ◽  
Vol 7 (5) ◽  
pp. 1431-1443
Author(s):  
Hong Zhang ◽  
Zhu Chen ◽  
Qing Huang
Keyword(s):  
Gene Expression ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Aquatic Environment ◽  
Zinc Oxide Nanoparticles ◽  
Photosynthetic Efficiency ◽  
Gene Expression Analysis ◽  
Chlorella Sorokiniana ◽  
Oxide Nanoparticles ◽  
Zno Nps

Zinc oxide nanoparticles (ZnO NPs) are one of the most abundantly applied nanomaterials in nanotechnology-based industries, and recent research continues to highlight their transformations and potential eco-toxicity especially to aquatic environment.

Microbiote shift in sequencing batch reactors in response to antimicrobial ZnO nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (111) ◽  
pp. 110108-110111 ◽  
Author(s):  
Zhenghui Liu ◽  
Huifang Zhou ◽  
Jiefeng Liu ◽  
Xudong Yin ◽  
Yufeng Mao ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Wastewater Treatment ◽  
Adverse Effects ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Wastewater Treatment Plants ◽  
Oxide Nanoparticles ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Zno Nps

Zinc oxide nanoparticles (ZnO NPs) have been monitored in wastewater treatment plants as their potential adverse effects on functional microorganisms have been causing increasing concern.

Electrochemical properties of green synthesised Zinc oxide (ZnO) Nanoparticles

MRS Advances ◽  
2020 ◽  
Vol 5 (21-22) ◽  
pp. 1103-1112
Author(s):  
G.G. Welegergs ◽  
H.G. Gebretinsae ◽  
R. Akoba ◽  
N. Matinsie ◽  
Z. Y. Nuru ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Electrochemical Properties ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Papaver Somniferum ◽  
Toxic Chemicals ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Bode Plot ◽  
Green Approach

AbstractBio-reduction agents are being explored to synthesised nanoparticles to minimize the effects of toxic chemicals. The present study was focused on green approach for the synthesis of zinc oxide nanoparticles using aqueous seeds extract of Papaver somniferum. The biosynthesised ZnO NPs (27.8nm) were characterized by using of spectroscopy and microscopy instruments. The surface morphology and the structural analysis confirms the formation of hexagonal nanostructure and a pure zincite nature of ZnO nanoparticles (NPs) respectively. The EDS spectrum confirms pure ZnO NPs were synthesised. From electrochemical properties, the CV indicates both anodic and cathodic sweep are quasi-reversible properties whose intensity increases with the scan rates. The bode plot shows the maximum angles of 74o which is an indication of a higher conductivity of ZnO NPs.

Biosynthesis and Characterization of Zinc Oxide Nanoparticles for the Systemic Treatment of Patients with Lymphoma in Nursing Care

2020 ◽  
Vol 12 (1) ◽  
pp. 137-143
Author(s):  
Lingling Meng ◽  
Lina Du ◽  
Yaqiong Shen ◽  
Shan Cong ◽  
Qiuyan Zhai ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Systemic Treatment ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Environment Friendly ◽  
Oxide Nanoparticle ◽  
T Lymphoma

Zinc oxide (ZnO) nanoparticles recently are of significant consideration because of their applications as nontoxic metal oxides. This study is mainly intended to improve a simple, efficient, and environment-friendly method for preparation of ZnO nanoparticles. This process has been developed based on plant-intervened synthesis by making use of microwave Saturejahortensis aqueous extract. We have further characterized the obtained Zinc oxide nanoparticles by using different techniques. Additionally, their cytotoxic potential was inspected via MTT assay against both B lymphoma, A20 and T lymphoma, EL4 cells lines. The zinc oxide nanoparticle exposed cells for about 24 h showed diminished cell viability in the tested cell lines where EL4 cells has been reduced to 75% of control after 24 h, whereas A20 cells were lowered to 68% of control. ZnO-NPs have proficiently hindered EL4 cell DNA synthesis at 100 μg concentration whereas completely blocked the DNA proliferation at about 50 g concentration. However, A20 cells besides presented similar propensity, thus exploring the ZnO-NPs potential to treat lymphomas.

scholarly journals Long-Term Impact of Zinc Oxide Nanoparticles on Differentiation and Cytokine Secretion of Human Adipose-Derived Stromal Cells

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1823 ◽  
Author(s):  
Katrin Radeloff ◽  
Andreas Radeloff ◽  
Mario Ramos Tirado ◽  
Agmal Scherzad ◽  
Rudolf Hagen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Oxide ◽  
Stromal Cells ◽  
Zinc Oxide Nanoparticles ◽  
Caspase 3 ◽  
Cytokine Secretion ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
The Impact

Zinc oxide nanoparticles (ZnO-NPs) are widely utilized, for example in manufacturing paints and in the cosmetic industry. In addition, there is raising interest in the application of NPs in stem cell research. However, cytotoxic, genotoxic and pro-inflammatory effects were shown for NPs. The aim of this study was to evaluate the impact of ZnO-NPs on cytokine secretion and differentiation properties of human adipose tissue-derived stromal cells (ASCs). Human ASCs were exposed to the subtoxic concentration of 0.2 µg/mL ZnO-NPs for 24 h. After four weeks of cultivation, adipogenic and osteogenic differentiation procedures were performed. The multi-differentiation potential was confirmed histologically and using polymerase chain reaction (PCR). In addition, the gene expression of IL-6, IL-8, vascular endothelial growth factor (VEGF) and caspase 3 was analyzed. Over the course of four weeks after ZnO-NPs exposure, no significant differences were detected in the gene expression of IL-6, IL-8, VEGF and caspase 3 compared to non-exposed cells. The differentiation was also not affected by the ZnO-NPs. These findings underline the fact, that functionality of ASCs is likely to be unaffected by ZnO-NPs, despite a long-term disposition of NPs in the cells, supposing that the starting concentration was safely in the non-toxic range. This might provide important information for single-use nanomedical applications of ZnO-NPs.

scholarly journals Mechanistic Study of Antibacterial Properties of Chemically Synthesize Zinc Oxide Nanoparticles

2019 ◽  
Vol 2 (1) ◽  
pp. 42-52
Author(s):  
Abdur Rehman ◽  
Saira Ahmad ◽  
Abdul Mateen ◽  
Huma Qamar ◽  
Mudaber Ahmad Mubashar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Bacterial Strain ◽  
Zinc Oxide Nanoparticles ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Mechanistic Study ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Wide Range

Nanotechnology is the science, engineering and technology conducted at the scale that ranges between 1-100 nanometers. For the bio-application, evolution of nanotechnology is creating the concern of scientists towards the synthesis of nanoparticles. The nanoparticles have unique characteristics as compare to bulk materials. Zinc oxide (ZnO) is a matchless semiconductor and it has been under investigation due to its wide range of applications in various areas like biomedical, electronics, material science and optics. In the present work synthesis of ZnO nanoparticles was carried out by using simple chemical approach, Sol-gel method for being effective and inexpensive, by employing zinc acetate dehydrate Zn (CH3CO2)2.2H2O as a precursor and sodium hydroxide (NaOH) starch as a constant agent. The structural properties of resultant zinc oxide nanoparticles were investigated by X-ray diffraction (XRD) technique. The XRD data confirmed the hexagonal wurtzite structure of ZnO powder confirmed by JCPDS 36-1451 data. Particles size was calculated by Scherrer formula and calculated size was 30.14 nm. These nanoparticles were investigated for inhibition zone of bacterial strain Escherichia coli, a gram-negative microbe, at various concentrations of ZnO nanoparticles. Zinc oxide nanoparticles were very proficient for inhibition of growth of bacterial strain E. coli. The mechanism of ZnO NPs for antibacterial activity is release of reactive oxygen species which not only hydrolyze cell wall but cell membrane and cellular components as well providing a potential bactericidal effect.

scholarly journals Biosynthesis and Characterization of Zinc Oxide Nanoparticles Using Leaf Extract of Phoenix Dactylifera and Their Impact on Biomass and Bioactive Compounds in Juniperus Procera in Vitro

2021 ◽  
Author(s):  
Abdalrhaman Salih ◽  
Fahad Al Qurainy ◽  
Salim Khan ◽  
Mohamed Tarroum ◽  
Mohammad Nadeem ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Biochemical Parameters ◽  
Phoenix Dactylifera ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Phytochemical Compounds ◽  
Juniperus Procera

Abstract Biosynthesized nanoparticles have played vital role recently, as suggested to be alternative to physical and chemical methods. In this study, biosynthesis of zinc oxide nanoparticles (ZnO NPs) were carried out using leaf extracts of Phoenix dactylifera and Zinc nitrate. The effect of ZnO nanoparticles on biomass and biochemical parameters was investigated. Biosynthesized ZnO nanostructure was characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–visible spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 to 35 nm of Biosynthesized ZnO nanoparticles and UV absorption beak at 370.5 nm with clear peaks of functional groups. The impact of different concentrations (0.0 mg/L, 80 mg/L and 160 mg/L) of biosynthesized ZnO nanoparticles on biomass and bioactive compounds production of Juniperus Procera in vitro was investigated. The results showed that, biosynthesized ZnO NPs (80 mg /L and 160 mg/L) concentrations were boosted the growth of J. Procera with significantly compared to non-treated plants in vitro. The highest concentration (160mg/L) of ZnO NPs was enhanced the growth of plant at beginning period, one month later shoots became yellow and callus turned to be brownish. Moreover, the influence of ZnO NPs on phytochemical compounds in callus of Juniperus procera was examined using GC-MS analysis. The differences among treatments were recoded. Overall, zinc oxide nanoparticles substantially improved the growth of shoots and callus with increasing of biochemical parameters such as chlorophyll a, total phenolic and flavonoids contents, besides the total protein and, SOD, CAT and APX activity. ZnO NPs might be induced some phytochemical compounds as well as inhibit.

Biosynthesis of ZnO Nanostructures Using Azadirachta indica Leaf Extract and Their Effect on Seed Germination and Seedling Growth of Tomato: An Eco-Friendly Approach

2020 ◽  
Vol 15 (11) ◽  
pp. 1412-1422
Author(s):  
Nishat Arshi ◽  
Y. Prashanthi ◽  
Tentu Nageswara Rao ◽  
Faheem Ahmed ◽  
Shalendra Kumar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Seed Germination ◽  
Azadirachta Indica ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
The Impact

In this study, we report synthesis of Zinc oxide nanoparticles using simple chemical and green methods. The ZnO nanoparticles were synthesized using leaf extract of Azadirachta indica (neem) as reducing agent. The as obtained product was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray analysis (EDAX) and TEM techniques. XRD analysis confirms that ZnO nanoparticles were crystalline having hexagonal Wurtzite structure with (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0) and (1 1 2) planes. SEM analyses show that the as synthesized ZnO NPs were in the form of agglomerates and no other impurity peak was found in the EDS. TEM analyses confirm that the size of the nanoparticle was approx. 50 nm. Here in, we investigate the effect of chemical and green synthesized zinc oxide nanoparticles on germination and growth of lycopersicum esculentus (tomato) using petri plate seed germination method in loamy sand soil. The impact of concentration of applied ZnO nanoparticles via green synthesis and chemical methods were analyzed. Results revealed that green synthesized Zinc oxide nanoparticles showed maximum growth of seedling as compared to chemically synthesized Zinc oxide nanoparticles, bulk ZnO and control. After 50 days of tomato growth analysis, it was recognized that ZnO NPs can be a good green synthetic fertilizer by increasing shoot length, wet weight, dry weight and yield over conventional control. Hence, green method is found to be more effective.

scholarly journals Zinc Oxide Nanoparticles Cytotoxicity and Release from Newly Formed PMMA–ZnO Nanocomposites Designed for Denture Bases

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1318 ◽  
Author(s):  
Mariusz Cierech ◽  
Jacek Wojnarowicz ◽  
Adam Kolenda ◽  
Agata Krawczyk-Balska ◽  
Emilia Prochwicz ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Host Cells ◽  
Emission Spectrometry ◽  
Oxide Nanoparticles ◽  
Pure Pmma ◽  
Zno Nps ◽  
Cytotoxicity Studies ◽  
The Impact

The goal of the study was to investigate the level of zinc oxide nanoparticles (ZnO NPs) release from polymethyl methacrylate (PMMA)–ZnO nanocomposites (2.5%, 5%, and 7.5% w/w), as well as from the ZnO NPs layer produced on pure PMMA, and the impact of the achieved final ZnO NPs concentration on cytotoxicity, before the potential use as an alternative material for denture bases. The concentration of ZnO nanoparticles released to the aqueous solution of Zn2+ ions was assessed using optical emission spectrometry with inductively coupled plasma (ICP-OES). In the control group (pure PMMA), the released mean for ZnO was 0.074 mg/L and for individual nanocomposites at concentrations of 2.5%, 5%, and 7.5% was 2.281 mg/L, 2.143 mg/L, and 3.512 mg/L, respectively. The median for the ZnO NPs layer produced on PMMA was 4.878 mg/L. In addition, in vitro cytotoxicity of ZnO NPs against the human HeLa cell line was determined through the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye. The cytotoxicity studies demonstrate that ZnO nanoparticles in the concentrations up to 20 mg/L have no adverse effect on HeLa cells. When compared with the released and cytotoxic concentrations of ZnO NPs, it can be expected that ZnO released from dental prostheses to the oral cavity environment will have no cytotoxic effect on host cells.

scholarly journals Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4682 ◽  
Author(s):  
Sinouvassane Djearamane ◽  
Yang Mooi Lim ◽  
Ling Shing Wong ◽  
Poh Foong Lee
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Viability ◽  
Aquatic Environment ◽  
Zinc Oxide Nanoparticles ◽  
Algal Biomass ◽  
Time Dependent ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Cytotoxic Effects

BackgroundThe extensive usage of zinc oxide nanoparticles (ZnO NPs) in industrial and consumer products raises the risk of releasing their residues into the aquatic environment. The presence of ZnO NPs in the aquatic environment could potentially cause cytotoxic effects on aquatic organisms. Thus, investigating the cytotoxic effects of ZnO NPs on microalgae, which form the base for the food web of aquatic biota, is essential to gain information regarding the ecotoxicological effects of metallic oxide nanoparticles in the aquatic ecosystem. Therefore, the present study has investigated in detail the assorted cytotoxic effects of ZnO NPs onS. platensisusing various concentrations of ZnO NPs (10–200 mg/L) from 6 to 96 h to explore the dose- and time-dependent cytotoxic effects.MethodsThe cytotoxic effects were all assessed through quantification of loss in cell viability, reduction in biomass and decrease in photosynthetic pigments such as chlorophyll-a, carotenoids and phycocyanin. The surface interactions of nanoparticles and the subsequent morphological alterations on algal cells were examined by optical and scanning electron microscopy (SEM). The intracellular alterations of algal cells were studied using transmission electron microscopy. Furthermore, Fourier transformed infrared (FTIR) spectrum was obtained to investigate the involvement of algal surface biomolecules in surface binding of ZnO NPs on algal cells.ResultsThe treatment of ZnO NPs onS. platensisexhibited a typical concentration- and time-dependent cytotoxicity. Results showed a significant (p < 0.05) cytotoxicity from 24 h onwards for all tested concentrations of ZnO NPs. The maximum cytotoxicity on algal cells was achieved at 96 h of exposure to ZnO NPs. In comparison with control, the algal cells that interacted with 200 mg/L of ZnO NPs for 96 h showed 87.3 ± 1% loss in cell viability, 76.1 ± 1.7% reduction in algal biomass, 92.5 ± 2.2%, 76.2 ± 2.2% and 74.1 ± 3.4% decrease in chlorophyll-a, carotenoids and phycocyanin contents respectively. Our study confirmed the cytotoxicity of ZnO NPs through the algal growth inhibition with 72 h EC10and EC50values of 1.29 and 31.56 mg/L, respectively. The microscopic examinations of the algal cells that interacted with ZnO NPs showed severe cell membrane and intracellular damage. The SEM EDX spectrum of ZnO NPs treated algal biomass evidenced the surface accumulation of zinc in the biomass. Finally, the FTIR spectrum confirmed the involvement of amino, hydroxyl and carboxylic groups of algal cell wall in the surface interaction of ZnO NPs on the algal cells.DiscussionThe results showed that the treatment of ZnO NPs onS. platensistriggered substantial cytotoxicity and caused cell death. Hence,S. platensiscould be potentially used as a bioindicator for testing toxicity of ZnO NPs in aquatic environment.

scholarly journals Reusability performance of green zinc oxide nanoparticles for photocatalysis of bathroom greywater

2020 ◽  
Author(s):  
G. Yashni ◽  
Adel Al-Gheethi ◽  
Radin Mohamed ◽  
Mohammed Al-Sahari
Keyword(s):  
Zinc Oxide ◽  
Wastewater Treatment ◽  
Zno Nanoparticles ◽  
Wastewater Treatment Plant ◽  
Zinc Oxide Nanoparticles ◽  
Treatment Plant ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Fixed Film

Abstract This study aims to investigate the potential of green zinc oxide nanoparticles (ZnO NPs) in reusability performance for photocatalysis of bathroom greywater. It was found that ZnO NPs photocatalytic treatment of real bathroom greywater (RBGW) effluent reduces both the COD and BOD5 concentrations by 72.01, 62.75 and 57.79% (COD) and 70.18, 60.32 and 57.56% (BOD5) respectively for the first, second and third cycle. Meanwhile for the photocatalysis of ABGW, it was observed that COD and BOD5 were removed by 82.27, 68.27 and 60.96% (COD) and 82.91, 74.37 and 60.39% (BOD5) for the first, second and third cycle respectively. Besides, TSS and turbidity were reduced by 52.34, 46.85 and 37.98% (TSS) and 80.38, 67.65 and 56.81% (turbidity) respectively in RBGW and for ABGW, TSS and turbidity were reduced by 60.94, 52.37 and 41.95% (TSS) and 80.68, 72.63 and 69.91% (turbidity) for the first, second and third experimental run respectively. This designates that green ZnO NPs can be reused multiple times as an effective photocatalyst. However, ZnO NPs was used in the dispersed form which causes difficulty in the separation of ZnO NPs from the greywater. Thus, further investigation in producing appropriate fixed film should be focused. Moreover, phytotoxicity analysis of V. radiate seeds in the treated ABGW and RBGW was not significantly different from their germination in water. This assured the less lethal nature of the degradation metabolites in greywater effluent. This investigation would be a solution to wastewater treatment plant for reusing photocatalyst in order to achieve the development of advanced and greener technologies. Highlight We would like to submit a manuscript titled ‘Reusability performance of green zinc oxide nanoparticles for photocatalysis of bathroom greywater’ to your Journal for possible evaluation. In this article, the potential of green ZnO Nanoparticles in degrading bathroom greywater discussed. The reusability performance of green zinc oxide nanoparticles for photocatalysis of bathroom greywater also discussed.

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