Flaxseed extrusion and expansion coupled with enzyme and pelleting changed protein and lipid molecular structure of flaxseed and improved digestive enzymes activity, intestinal morphology, breast muscle fatty acids and performance of broiler chickens

Duarte, C. R. A., Eyng, C., Murakami, A. E. and Santos, T. C. 2014. Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis. Can. J. Anim. Sci. 94: 105–114. Propolis is a resin produced by bees that has been investigated for its potential to increase the performance of broiler chickens. Therefore, this study aimed to evaluate the inclusion of crude propolis in the diets of broiler chickens from 1 to 21 d of age. A total of 1020 male chicks were distributed in a completely randomized experimental design into six treatments (0, 100, 200, 300, 400 and 500 ppm of crude propolis), with five replications, and 34 birds per experimental unit. The experimental diets were provided from 1 to 21 d of age and subsequently (from 22 to 42 d of age) the birds were fed a nutritionally complete ration based on corn and soybean meal. All supplemented groups showed similar growth performance in all growth phases (P>0.05); however, there was a linear increase in carcass yield at 42 d of age according to increase in crude propolis inclusion from 1 to 21 d of age (P<0.05). At 7 d of age, relative weight of the gizzard increased linearly (P<0.05) with increasing propolis, and at 21 d of age, relative weight of the large intestine showed a quadratic (P<0.05) response, with the lowest value predicted to occur at 278 ppm of crude propolis. The ileum crypt depth at 21 d of age was affected by propolis, with a quadratic response (P<0.05) and highest value estimated at 323 ppm. At 21 d of age, sucrase activity in the jejunum increased linearly with increasing propolis and maltase activity in the ileum showed a quadratic (P<0.05) response, which predicted highest activity at 292 ppm. Amylase activity was lower in animals supplemented with 100 and 400 ppm of propolis at 21 d of age (P<0.05) compared with the control group. Thus, the crude propolis supplementation is not recommended in broiler diets from 1 to 21 d old given that it does not influence the performance, despite small changes in intestinal morphophysiology at 21 d of age.

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Effects of an organic acid mixture and methionine supplements on intestinal morphology, protein and nucleic acids content, microbial population and performance of broiler chickens

Animal Production Science ◽

10.1071/an11081 ◽

2011 ◽

Vol 51 (11) ◽

pp. 1025 ◽

Cited By ~ 1

Author(s):

A. A. Saki ◽

S. M. Eftekhari ◽

P. Zamani ◽

H. Aliarabi ◽

M. Abbasinezhad

Keyword(s):

Nucleic Acids ◽

Organic Acids ◽

Organic Acid ◽

Dna Content ◽

Broiler Chickens ◽

Microbial Population ◽

Intestinal Morphology ◽

Acid Mixture ◽

Broiler Chicks ◽

And Performance

A study was carried out to investigate the effects of dietary supplementation of an organic acids mixture and two methionine supplements on intestinal morphology, protein and nucleic acids content, microbial population and performance of broiler chickens. Six hundred unsexed day-old Ross 308 broiler chicks were used in a factorial arrangement (3 × 2) based on a completely randomised design by four replicates with 25 chicks in each. Experimental diets consisted of: 0% organic acid mixture + DL-methionine (Treatment 1), 0% organic acid mixture + Alimet (Treatment 2), 0.5% organic acid mixture + DL-methionine (Treatment 3), 0.5% organic acid mixture + Alimet (Treatment 4), 1% organic acid mixture + DL-methionine (Treatment 5) and 1% organic acid mixture + Alimet (Treatment 6). A significant increase in jejunum mucosal DNA content was found by using 1% of organic acid mixture compared with those by other levels of organic acids at 21 days of age (P < 0.05). Also Treatments 5 and 6 had a higher mucosal DNA content than other treatments (P < 0.05) in a similar period. While at grower stage, the results have shown that protein content, protein/DNA and protein/RNA ratio by 0.5 and 1% of organic acids mixture significantly increased compared with those in the 0% level (P < 0.05). The addition of 1% level of organic acid mixture significantly increased villus height compared with 0 and 0.5% level of organic acid mixture at 21 days of age (P < 0.05). Highest and lowest villus heights were obtained by Treatments 1 and 6 (P < 0.05). Crypt depth increased by 0.5 and 1% of organic acid at 21 and 42 days of age (P < 0.05). Villus surface was significantly increased by 1% of organic acid mixture compared with that by 0% level at 21 days of age. The use of organic acid levels especially 1% resulted in an increase in lactic acid bacteria and decreased Enterobacteriaceae counts in the ileum of broiler chicken (P < 0.05). Better bodyweight and feed conversion ratio were obtained (P < 0.05) by 0.5 and 1% of organic acids mixture, although no response was found in feed intake and mortality in this respect (P > 0.05). The results of this study have shown improved reactions on intestinal morphometrical parameters and microflora atmosphors, by using 1% organic acid mixture. In contrast no differences were found between Alimet and DL-methionine in all tested parameters.

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Effect of in ovo injected prebiotics and synbiotics on the caecal fermentation and intestinal morphology of broiler chickens

Animal Production Science ◽

10.1071/an16257 ◽

2017 ◽

Vol 57 (9) ◽

pp. 1884 ◽

Cited By ~ 9

Author(s):

D. Miśta ◽

B. Króliczewska ◽

E. Pecka-Kiełb ◽

V. Kapuśniak ◽

W. Zawadzki ◽

...

Keyword(s):

Lactococcus Lactis ◽

Broiler Chickens ◽

Intestinal Morphology ◽

Jejunal Mucosa ◽

In Ovo ◽

Lactococcus Lactis Subsp ◽

Crypt Depth ◽

Molar Proportion ◽

Depth Ratio ◽

And Performance

Manipulations of the intestinal microbiota composition may improve the health and performance of chickens. In ovo technology allows the administration of a bioactive substance to enter directly into the incubating egg. The objective of the present study was to investigate the effect of in ovo administered prebiotics or synbiotics on microbial activity products in the caeca and the development of the small intestine of broiler chickens. Ross 308 male chickens hatched from eggs injected in ovo with prebiotics or synbiotics were used in this study. Five experimental groups were formed: C (Control) – injected with 0.9% NaCl, the Pre-1 and Pre-2 groups – injected with prebiotics: inulin or Bi2tos, respectively, and the Syn-1 and Syn-2 groups – injected with synbiotics: inulin with Lactococcus lactis subsp. lactis IBB SL1 or Bi2tos with Lactococcus lactis subsp. cremoris IBB SC1, respectively. At the age of 7, 21 and 35 days, 10 chicks of each group were randomly selected, weighed and slaughtered, and the jejunal samples were collected for histological examinations, whereas caecal samples were collected to analyse the end products of microbial fermentation. Synbiotic treatment increased bodyweight, as observed in the Syn-1 group (P < 0.05). The propionate molar proportion was highest in the groups treated with synbiotics, especially in the Syn-1 group (P < 0.01). Furthermore, the molar proportion of acetate was also lowest in the Syn-1 group (P < 0.05). In ovo synbiotics treatment increased the villus length : crypt depth ratio in the jejunal mucosa, which might improve nutrient absorption and contribute to the increased weight of chickens. These effects suggest that the in ovo administration of synbiotics may be an effective method to increase bodyweight, improve the short-chain fatty acid caecal profile and increase the villus length : crypt depth ratio in the jejunal mucosa. These effects were more pronounced in the Syn-1 group than the Syn-2 group.

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Fatty acid profile, antioxidative status and dietary value of the breast muscle of broiler chickens receiving glycine-Zn chelates

Animal Production Science ◽

10.1071/an19305 ◽

2020 ◽

Vol 60 (8) ◽

pp. 1095

Author(s):

Anna Winiarska-Mieczan ◽

Małgorzata Kwiecień ◽

Katarzyna Kwiatkowska ◽

Ewa Baranowska-Wójcik ◽

Dominik Szwajgier ◽

...

Keyword(s):

Fatty Acids ◽

Broiler Chickens ◽

The Body ◽

Breast Muscle ◽

Atherogenic Index ◽

Control Group ◽

Breast Meat ◽

Antioxidative Status ◽

Fatty Acids Profile ◽

Zn Chelates

In the present study we aimed to examine the effect of glycine-Zn chelates (ZnGly) used in amounts corresponding to 0, 100, 50 or 25% of the requirement of this element on the fatty acids profile, antioxidative status, and dietetic value (atherogenic index (AI), thrombogenic index (TI), hypocholesterolemic/hypercholesterolemic ratio (h/H)) of breast muscles in broiler chickens. Two hundred 1-day-old Ross 308 male chicks were split into four equipotent experimental groups (assigned to 20 cages, 10 chicks in each, replicated five times): control, ZnGly-100, ZnGly-50 and ZnGly-25. The broilers received feed and water ad libitum. The experiment lasted 42 days. Significant differences in the content of respective fatty acids in the meat of chickens receiving ZnGly were observed in comparison to the control group; but they were not directional changes. A positive effect (P < 0.05) of Zn-Gly on certain indices of meat determined by the oxidoreductive status of the body or regulating such status was noted: superoxide dismutase, catalase and malondialdehyde. A significant effect of ZnGly on the dietetic value of broiler chickens’ breast meat was noted: the highest (P < 0.05) AI value was found in the ZnGly-50 and ZnGly-25 groups, and TI value in the ZnGly-25 group. The h/H relationship can be presented as ZnGly-25 < ZnGly-50 < ZnGly-100 < control group. To improve the dietetic value and to increase the oxidative stability of breast muscle meat, broiler chickens must receive ZnGly in an amount corresponding to more than 25% of the requirement of this element.

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