Can laboratory toxicity tests explain the pattern of field communities of algae, plants, and invertebrates along a toxicity gradient of post-mining sites?

2011 ◽  
Vol 51 ◽  
pp. 114-121 ◽  
Author(s):  
Jan Frouz ◽  
Kristýna Hrčková ◽  
Jan Lána ◽  
Václav Krištůfek ◽  
Ondřej Mudrák ◽  
...  
Keyword(s):  
Toxicity Tests ◽  
Mining Sites

Problems in Detoxifying Fresh Waters Polluted by Base Metal Minewaters

1971 ◽  
Vol 6 (1) ◽  
pp. 53-79
Author(s):  
Vaclav Kresta ◽  
Gerald B. Ward
Keyword(s):  
Chelating Agents ◽  
Toxicity Tests ◽  
Contaminated Water ◽  
Total Dosage ◽  
Toxic Compounds ◽  
Mining Sites ◽  
Know How ◽  
Chemical Agents ◽  
Copper And Zinc ◽  
Receiving Waters

Abstract At many mining sites process (milling) and drainage waters escape treatment and cause receiving waters to become contaminated above avoidance or even toxic levels for fish. The present know-how on chemical agents which can be used to complex with copper and zinc to form non-toxic compounds is limited to chelating agents such as NTA or EDTA. Preferential reaction with trivalent ions such as iron means that such ions must be tied up before complexation of copper and zinc can occur. As the amount of iron in contaminated water is usually two to eight times higher than that of copper and zinc, high dosages of chelating agents are usually required. In this project, the use of salts of anthranilic acid, especially calcium anthranilate, was investigated. The consumption of anthranilateions was found to be about the same as that of NTA or EDTA, i.e. four milligrams per milligram of copper or zinc. The total dosage to be applied to contaminated waters would be, however, several times lower as iron is not involved in the reactions and copper and zinc are complexed in that order. Toxicity tests to compare the efficiency and dasages of calcium anthranilate and NTA or EDTA are presently being carried out.

Study of the radiomodifier effect of Pityrocarpa moniliformis extract

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
João Victor Torres de Moraes ◽  
Ricardo Luiz Calazans Luna Filho ◽  
Williams Nascimento de Siqueira ◽  
Hianna Arely Milca Fagundes Silva ◽  
Dewson Rocha Pereira ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Cellular Response ◽  
Biomphalaria Glabrata ◽  
Toxicity Tests ◽  
Antioxidant Compounds ◽  
Blastula Stage ◽  
Natural Substances ◽  
60Co Source ◽  
60Co Gamma ◽  
60Co Gamma Radiation

Ionizing radiation has been applied in several areas of knowledge, among them the study of the radiomodifier activity of natural substances. These substances can modify the cellular response to the damage induced by the radiation. Therefore, this work aimed to evaluate the radiomodifier action of Pityrocarpa moniliformis extract on Biomphalaria glabrata embryos exposed to 60Co gamma radiation. Initially, toxicity tests were performed on the extract against the B. glabrata embryos for the choice of concentration that did not cause death and embryonic malformation. Then, the antioxidant activity of the P. moniliformis extract with flavonoids and phenolic compounds was evaluated by means of the ABTS method. To evaluate the radiomodifier activity of the extract, embryos were selected in the blastula stage and irradiated with 7.5 Gy in a 60Co source (gammacell-Co60). Then, the embryos were exposed for 24 h to the extract of P. moniliformis at a concentration of 250 μg/mL. The results showed that the extract of P. moniliformis presents flavonoids and enzymatic inhibition by ABTS, which demonstrates the presence of antioxidant compounds. However, the tests of the radiomodifier activity did not present radioprotective effect for embryos exposed to ionizing radiation.

Acute and chronic effect of cyaniade on survival rate, behavior, and reproduction of Daphnia sp.

2013 ◽  
Vol 1 (1) ◽  
pp. 52 ◽  
Author(s):  
Melky R Pattiwael ◽  
Remy E. P Mangindaan ◽  
Rudi Prabowo ◽  
Inneke F. M Rumengan
Keyword(s):  
Government Regulation ◽  
Aquatic Organisms ◽  
Standard Test ◽  
Toxicity Tests ◽  
Zooplankton Organism ◽  
Rate Behavior ◽  
Negative Impacts ◽  
Reproduction Pattern ◽  
Acute And Chronic Effects ◽  
Ministerial Decree

Cyanide (CN) through leaching process in the gold processing could produce tailings that have negative impacts on the aquatic environment. To determine the acute and chronic effects of cyanide on aquatic organisms, toxicity tests have been conducted in the laboratory using Daphnia sp.This zooplankton organism is recommended by APHA as standard test animals. In this study the test concentrations refer to the Ministerial Decree No. 202 of 2004 and Government Regulation No. 82 of 2001. Cyanide was analyzed as free CN and WADS CN. The result of acute toxicity test using cyanide solution showed that Daphnia could survive a maximum concentration of 0.2 ppm. LC50 values for 24 and 72 hours were 0,59 mg/L and 0,10 mg/l, respectively. The LT50 was found at 42 hours. Daphnia sp. produced different numbers of offspring at different CN concentrations, and changed their reproduction pattern from parthenogenesis to sexual reproduction after exposure to a cyanide concentration of 0.1 ppm for 24 hours©  Sianida (CN) melalui proses leaching dalam proses pengolahan emas menghasilkan limbah yang dapat memberi dampak negatif bagi lingkungan perairan. Untuk mengetahui sampai seberapa jauh CN dapat memberi efek akut dan kronis terhadap biota air, telah dilakukan uji toksisitas di laboratorium dengan menggunakan Daphnia sp. Zooplankton ini direkomendasikan oleh APHA sebagai salah satu hewan uji standar. Dalam penelitian ini konsentrasi uji yang dipakai mengacu pada peraturan yang berlaku, yaitu Keputusan Menteri No. 202, Tahun 2004, dan Peraturan Pemerintah No. 82, Tahun 2001. Analisis sianida yang dihitung adalah nilai free CN dan WADS CN. Hasil penelitian uji toksisitas akut dengan menggunakan larutan sianida didapati Daphnia mampu bertahan hidup sampai pada konsentrasi 0,2 ppm dan nilai LC50 berada pada konsentarsi 0,1 ppm serta LT50 pada jam ke 42. Hasil uji efek kronis, Daphnia sp. menghasilkan jumlah anakan yang berbeda pada konsentrasi CN yang berlainan, dan mengalami perubahan pola reproduksi dari partenogenesis menjadi seksual setelah dipaparkan pada kosentrasi sianida 0,1 ppm selama 24 jam©

Heavy metals pollution potentials in the National Iron Ore Mining Company, Itakpe

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Cyril Ocheri ◽  
A. D. Omah ◽  
C. N. Mbah ◽  
R. E. Njoku ◽  
N. A. Urama ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Sea Level ◽  
Toxic Elements ◽  
Iron Ore ◽  
Pollution Potential ◽  
Extraction Techniques ◽  
Mining Company ◽  
Mining Sites ◽  
Metals Pollution ◽  
Heavy Metals Pollution

Heavy metals pollution potential in National Iron Ore Mining Company, Itakpe was investigated. Two mining sites located at the east mining pits such as M3O, which is 370 m above sea level and M2O, which is 350 m above sea level were studied.  Sequential extraction techniques was utilised to examine the distribution effect of the heavy metals pollution potential on the environment. Twelve representative (six-soil, two-sediment, two-plant and two-water) samples were collected, pre-treated and prepared for this study. The atomic absorption spectrometer was used to analyse the concentration of the metals after the sequential and single-stage extractions were determined. Results showed that Chromium, Arsenic, Cadmium and Copper are more bioavailable in the study area than Lead and Iron. This findings indicate that human, animals and plants are exposed to toxic elements (metals and metalloids).

Metallic Elements (Cu, Zn, Ni and Mn) Toxicity Effects Determination on a Fresh Water Fish Cyprinus Carpio (Common Carp) Laboratory Acclimatized

2017 ◽  
Vol 68 (8) ◽  
pp. 1711-1715
Author(s):  
Stefania Gheorghe ◽  
Gabriela Geanina Vasile ◽  
Cristina Gligor ◽  
Irina Eugenia Lucaciu ◽  
Mihai Nita Lazar
Keyword(s):  
Cyprinus Carpio ◽  
Aquatic Organisms ◽  
Control Fish ◽  
Toxicity Tests ◽  
Fresh Water Fish ◽  
Metallic Elements ◽  
Mn Toxicity ◽  
Vitro Effect

Metallic elements copper (Cu), zinc (Zn), nickel (Ni) and manganese (Mn) are some of the most commonly found in water and sediment samples collected from the Danube - Danube Delta. These elements are important as essential micronutrients, being normally present at low concentrations in biological organisms, but in high concentrations they become toxic with immediate and delayed effects. The role of this metals is still controversial, that�s why bioconcentration potential is so important. In this non-clinical study, we tested in vitro effect of heavy metals on carp, Cyprinus carpio, reproducing in vivo presence of Cu, Zn, Ni and Mn in the Romanian�s surface water. The toxicity tests were performed according to OECD 203 by detecting the average (50%) lethal concentration - LC50 on aquatic organisms (freshwater fish) at 96h. The results pointed out that, copper value for LC 50 at 96h was estimated as 3.4 mg/L (concentrations tested in the range of 0.1 - 4.75 mg/L). Zinc value for LC 50 at 96h was estimated as 20.8 mg/L (concentrations tested in the range of 0.028 � 29.6 mg/L). Nickel value for LC 50 at 96h was estimated as 40.1 mg/L (concentrations tested in the range of 0.008 - 84.5 mg/L). For manganese the mortality effects has recorded at LC 50 at 96h at estimated value higher than 53 mg/L (concentrations tested in the range of 0.04 - 53.9 mg/L). The accuracy of the testing metals concentration was insured by the screening of the dilution water, as well as food and control fish, acclimated in laboratory conditions.

Report on Toxicity Tests on Mice: Report No. 41

1917 ◽  
Author(s):  
A. G. Kolls ◽  
H. A. Kuhn ◽  
A. J. Todd
Keyword(s):  
Toxicity Tests

Acute Toxicity Testing with Juvenile White Sturgeon (Acipenser transmontanus)

1998 ◽  
Vol 33 (1) ◽  
pp. 95-110 ◽  
Author(s):  
W.R. Bennett ◽  
A.P. Farrell
Keyword(s):  
Acute Toxicity ◽  
Swimming Performance ◽  
Juvenile Fish ◽  
Toxicity Testing ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Toxicity Tests ◽  
Fraser River ◽  
Acute Toxicity Tests ◽  
Acute Toxicity Testing

Abstract The primary goal of this study was to investigate the possibility of using early life stages of white sturgeon (Acipenser transmontanus) (eggs, larvae and fry) as a species relevant to the Fraser River, B.C., for the acute and sublethal toxico-logical testing of forest industry effluents. Here we report the first successful acute toxicity tests for 8-day-old larvae and 42-day-old fry exposed to several chemicals known to be released into the Fraser River (i.e., 6 monochlorovanillin [6 MVAN], 4,5 dichloroguaiacol [4,5 DCG], 4,5 dichlorocatechol [4,5 DCAT], pentachlorophenol [PCP], and didecyldimethylammonium chloride [DDAC]). In most cases, white sturgeon fry were at the lower end of the range for acute toxicity values for chlorinated phenolic compounds, when compared with other juvenile fish species, and they were extremely sensitive to DDAC. The larval stage was usually more sensitive than the fry stage. Acute toxicity tests with fertilized eggs were unsuccessful. A postexposure growth study was inconclusive because neither control nor toxicant-exposed larvae and fry withstood the additional handling used for measuring body mass. At 62-days-old, fry were more tolerant of handling. This allowed measurement of their swimming performance. Although we have concerns about the reliability of using larvae for acute toxicity testing at this time, 60-day-old white sturgeon fry would appear to be both a sensitive and relevant species for assessing environmental impacts relevant to the Fraser River.

Use of Biomonitoring to Control Toxics in the United States

1988 ◽  
Vol 20 (10) ◽  
pp. 101-108 ◽  
Author(s):  
Nelson A. Thomas
Keyword(s):  
Water Quality ◽  
Chronic Toxicity ◽  
National Policy ◽  
Single Species ◽  
Field Studies ◽  
The United States ◽  
Toxicity Tests ◽  
Toxic Pollutants ◽  
Receiving Waters ◽  
Biological Field

A biomonitoring program has been developed in support of the National Policy for the Development of Water Quality-Based Permit Limitations for Toxic Pollutants. The program focuses on the use of laboratory toxicity tests on aquatic plants and animals to predict ecosystem impact caused by toxic pollutants. Both acute and chronic toxicity tests were developed to test effluents and ambient waters. Laboratory and biological field studies were conducted at nine sites. Single species laboratory toxicity tests were found to be good predictors of impacts on the ecosystem when two or more species were used. Biomonitoring can be undertaken either on effluents and/or on the receiving waters. In that toxicity related to seeps, leachates and storm sewers has often been found upstream from dischargers, it is beneficial to conduct both effluent and ambient biomonitoring.

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