DNA damage response following X-irradiation in oral cancer cell lines HSC3 and HSC4

2018 ◽  
Vol 90 ◽  
pp. 1-8 ◽  
Author(s):  
Sirimanas Jiaranuchart ◽  
Atsushi Kaida ◽  
Yusuke Onozato ◽  
Hiroyuki Harada ◽  
Masahiko Miura
2011 ◽  
Vol 43 (7) ◽  
pp. 419 ◽  
Author(s):  
Kang Ho Kim ◽  
Hae Yong Yoo ◽  
Kyeung Min Joo ◽  
Yong Jung ◽  
Juyoun Jin ◽  
...  

The Prostate ◽  
2012 ◽  
Vol 72 (13) ◽  
pp. 1407-1411 ◽  
Author(s):  
Hisamitsu Ide ◽  
Yan Lu ◽  
Jingsong Yu ◽  
Toshiyuki China ◽  
Tomoka Kumamoto ◽  
...  

Author(s):  
Anirudh Menon ◽  
Vishnu Priya V ◽  
Gayathri R

<p>ABSTRACT<br />Objective: This study aims at performing a preliminary phytochemical analysis to evaluate the phytochemical composition of pineapple extract and<br />its cytotoxicity potential on oral cancer cell lines.<br />Methods: Preliminary phytochemical analysis of pineapple extract was done, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide<br />assay for evaluating the cytotoxicity potential of the extract on oral cancer cell lines was performed.<br />Results: Phytoconstituents such as flavonoids, coumarins, and phenols were present in the pineapple extract. The extract also exhibited increased<br />cytotoxicity with increased concentration.<br />Conclusion: This study is conducted to see if pineapple extract is effective in treating oral cancer in a natural way instead of harmful treatments.<br />Keywords: Cytotoxicity, Pineapple extract, Anticancer drug.</p><p> </p>


2011 ◽  
Vol 414 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Burcu Erbaykent-Tepedelen ◽  
Besra Özmen ◽  
Lokman Varisli ◽  
Ceren Gonen-Korkmaz ◽  
Bilge Debelec-Butuner ◽  
...  

2020 ◽  
Vol 295 (50) ◽  
pp. 17169-17186
Author(s):  
Mysore S. Veena ◽  
Santanu Raychaudhuri ◽  
Saroj K. Basak ◽  
Natarajan Venkatesan ◽  
Parameet Kumar ◽  
...  

We have observed overexpression of PACS-1, a cytosolic sorting protein in primary cervical tumors. Absence of exonic mutations and overexpression at the RNA level suggested a transcriptional and/or posttranscriptional regulation. University of California Santa Cruz genome browser analysis of PACS-1 micro RNAs (miR), revealed two 8-base target sequences at the 3′ terminus for hsa-miR-34a and hsa-miR-449a. Quantitative RT-PCR and Northern blotting studies showed reduced or loss of expression of the two microRNAs in cervical cancer cell lines and primary tumors, indicating dysregulation of these two microRNAs in cervical cancer. Loss of PACS-1 with siRNA or exogenous expression of hsa-miR-34a or hsa-miR-449a in HeLa and SiHa cervical cancer cell lines resulted in DNA damage response, S-phase cell cycle arrest, and reduction in cell growth. Furthermore, the siRNA studies showed that loss of PACS-1 expression was accompanied by increased nuclear γH2AX expression, Lys382-p53 acetylation, and genomic instability. PACS-1 re-expression through LNA-hsa-anti-miR-34a or -449a or through PACS-1 cDNA transfection led to the reversal of DNA damage response and restoration of cell growth. Release of cells post 24-h serum starvation showed PACS-1 nuclear localization at G1-S phase of the cell cycle. Our results therefore indicate that the loss of hsa-miR-34a and hsa-miR-449a expression in cervical cancer leads to overexpression of PACS-1 and suppression of DNA damage response, resulting in the development of chemo-resistant tumors.


2017 ◽  
Vol 84 ◽  
pp. 94-99 ◽  
Author(s):  
Hyun-Ju Yu ◽  
Ji-Ae Shin ◽  
In-Hyoung Yang ◽  
Dong-Hoon Won ◽  
Chi Hyun Ahn ◽  
...  

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