The Effect of Platelet Rich Plasma and Hyaluronic Acid on Autologous Osteochondral Transplantation: an in vivo Rabbit Model

Introduction Autologous osteochondral transplantation (AOT) is a treatment for osteochondral lesions with known concerns, including histological degradation of the graft and poor cartilage integration. Platelet-rich plasma (PRP) and hyaluronic acid (HA) have been described has having the potential to improve results. The aim of this study was to evaluate the effect of PRP and HA on AOT in a rabbit model. Methods Thirty-six rabbits underwent bilateral knee AOT treated with either the biological adjunct (PRP, n = 12; HA, n = 12; PRP + HA, n = 12) or saline (control). PRP and HA were administered as an intra-articular injection. The rabbits were euthanized at 3, 6, or 12 weeks postoperatively. The graft sections were assessed using the modified International Cartilage Repair Society (ICRS) scoring system. The results from the PRP alone group is from previously published data. Results The mean modified ICRS histological score for the PRP-treated group was higher than its control ( P = 0.002). The mean modified ICRS histological score for the HA-treated group showed no difference compared with its control ( P = 0.142). The mean modified ICRS histological score for the PRP + HA–treated group was higher than its control ( P = 0.006). There was no difference between the mean modified ICRS scores of the PRP- and the PRP + HA–treated grafts ( P = 0.445). Conclusion PRP may decrease graft degradation and improve chondral integration in an animal model. In this model, the addition of HA was not synergistic for the parameters assessed. Level of Evidence. Basic science, Level V. Clinical Relevance. PRP can be used as an adjunct to AOT, which may decrease graft degeneration and improve clinical outcomes. HA may not influence AOT.

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Sequential Changes in Implanted Cartilage After Autologous Osteochondral Transplantation: Postoperative Acoustic Properties up to 1 Year in an In Vivo Rabbit Model

Arthroscopy The Journal of Arthroscopic and Related Surgery ◽

10.1016/j.arthro.2007.01.012 ◽

2007 ◽

Vol 23 (6) ◽

pp. 647-654 ◽

Cited By ~ 12

Author(s):

Hiroshi Kuroki ◽

Yasuaki Nakagawa ◽

Koji Mori ◽

Masahiko Kobayashi ◽

Yukihiro Okamoto ◽

...

Keyword(s):

Rabbit Model ◽

Acoustic Properties ◽

Osteochondral Transplantation ◽

Autologous Osteochondral Transplantation

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In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model

Journal of Nanobiotechnology ◽

10.1186/s12951-021-01091-0 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Yuqing Niu ◽

Massimiliano Galluzzi ◽

Ming Fu ◽

Jinhua Hu ◽

Huimin Xia

Keyword(s):

Hyaluronic Acid ◽

Carotid Artery ◽

Structural Integrity ◽

Composite Scaffold ◽

Vascular Endothelial Cells ◽

Rabbit Model ◽

Vascular Reconstruction ◽

Vascular Prostheses ◽

Nanofibrous Scaffolds

AbstractOne of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract

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Influence of Intra-Articular Administration of Trichostatin A on Autologous Osteochondral Transplantation in a Rabbit Model

BioMed Research International ◽

10.1155/2015/470934 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Huacheng Hou ◽

Ke Zheng ◽

Guanghu Wang ◽

Shiro Ikegawa ◽

Minghao Zheng ◽

...

Keyword(s):

Articular Cartilage ◽

Cartilage Repair ◽

Trichostatin A ◽

Interleukin 1 ◽

Rabbit Model ◽

Treated Group ◽

Control Group ◽

Osteochondral Transplantation ◽

Autologous Osteochondral Transplantation ◽

Significant Difference

Autologous osteochondral transplantation (AOT) is a method for articular cartilage repair. However, several disadvantages of this method have been reported, such as transplanted cartilage degeneration and the lack of a connection between the grafted and adjacent cartilage tissues. To evaluate the effect of intra-articular administration of trichostatin A (TSA) on AOT, we conducted a case control study in a rabbit model. International Cartilage Repair Society (ICRS) macroscopic scores, the modified O’Driscoll histology scores, and real-time PCR were utilized to evaluate the results. At 4 weeks, both macroscopic and histological assessments showed that there was no significant difference between the TSA and control groups. However, the mean macroscopic and histological scores for the TSA-treated group were significantly higher than the scores for the control group at 12 weeks. TSA was shown to directly reduce collagen type II (COL2), aggrecan, matrix metalloproteinase (MMP), and a disintegrin and metalloproteinase domain with thrombospondin motifs 5 (ADAMTS-5) expression and to simultaneously repress the upregulation of MMP-3, MMP-9, and MMP-13 levels induced by interleukin 1β(IL-1β) in chondrocytes. In conclusion, TSA protects AOT grafts from degeneration, which may provide a benefit in the repair of articular cartilage injury.

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Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Journal of Clinical Medicine ◽

10.3390/jcm8091486 ◽

2019 ◽

Vol 8 (9) ◽

pp. 1486 ◽

Cited By ~ 12

Author(s):

Barbara De Angelis ◽

Margarida Fernandes Lopes Morais D’Autilio ◽

Fabrizio Orlandi ◽

Giampiero Pepe ◽

Simone Garcovich ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Platelet Rich Plasma ◽

Combined Treatment ◽

Skin Grafting ◽

Control Group ◽

In Vivo Evaluation ◽

Chronic Ulcers

Chronic ulcers are characterized by loss of substance without a normal tendency towards spontaneous healing. The Wound Bed Preparation Guideline advises that after diagnosis, the expert should correct the biological state of the ulcer micro-environment based on TIME principles (Tissue, Infection, Moisture balance, Epidermal). There are many ways to treat such ulcers, for example through use of advanced dressings, negative pressure, surgical toilets, dermal substitutes, autologous skin grafting, and free or local flaps. In vitro and in vivo pre-clinical models hold widely acknowledged potential yet complex limitations. Tissue bioengineering could be an ideal approach to foster innovative strategies in wound healing. Our observational study reports on an in vitro and in vivo evaluation of a bio-functionalized scaffold composed of platelet-rich plasma (PRP) and hyaluronic acid (HA) used in 182 patients affected by chronic ulcers (diabetic and vascular), comparing the results with a control group of 182 patients treated with traditional dressings (HA alone). After 30 days the patients who had undergone the combined treatment (PRP + HA), showed 96.8% ± 1.5% re-epithelialization, as compared to 78.4% ± 4.4% in the control group (HA only). Within 80 days, they had 98.4% ± 1.3% re-epithelialization as compared to 87.8% ± 4.1% in the control group (HA only; p < 0.05). No local recurrence was observed during the follow-up period. PRP + HA treatment showed stronger regenerative potential in terms of epidermal proliferation and dermal renewal compared with HA alone.

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In Vivo Engineering of the Vocal Fold Extracellular Matrix with Injectable Hyaluronic Acid Hydrogels: Early Effects on Tissue Repair and Biomechanics in a Rabbit Model

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348940511400902 ◽

2005 ◽

Vol 114 (9) ◽

pp. 662-670 ◽

Cited By ~ 84

Author(s):

Jennifer K. Hansen ◽

Susan L. Thibeault ◽

Jennifer F. Walsh ◽

Xiao Zheng Shu ◽

Glenn D. Prestwich

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Vocal Fold ◽

Wound Repair ◽

Rabbit Model ◽

Vocal Folds ◽

Biomechanical Properties ◽

Enzyme Linked Immunosorbent Assay ◽

Early Effects

Objectives: A prospective, controlled animal study was performed to determine whether the use of injectable, chemically modified hyaluronic acid (HA) derivatives at the time of intentional vocal fold resection might facilitate wound repair and preserve the unique viscoelastic properties of the vocal fold extracellular matrix. Methods: We performed bilateral vocal fold biopsies on 33 rabbits. Two groups of rabbits were unilaterally treated with 2 different HA derivatives — Carbylan-SX and HA-DTPH-PEGDA — at the time of resection. Saline was injected as a control into the contralateral fold. The animals were painlessly sacrificed 3 weeks after biopsy and injection. The outcomes measured included histologic fibrosis level, tissue HA level, and tissue viscosity and elasticity. Results: The Carbylan-SX—treated vocal folds were found to have significantly less fibrosis than the saline-treated controls. The levels of HA in the treated vocal folds were not significantly different from those in the controls at 3 weeks as measured by enzyme-linked immunosorbent assay. The Carbylan-SX—treated vocal folds had significantly improved biomechanical properties of elasticity and viscosity. The HA-DTPH-PEGDA injections yielded significantly improved viscosity, but not elasticity. Conclusions: Prophylactic in vivo manipulation of the extracellular matrix with an injectable Carbylan-SX hydrogel appears to induce vocal fold tissue regeneration to yield optimal tissue composition and biomechanical properties favorable for phonation.

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Effect of hyaluronic acid on osteoblast function in vivo and on fracture healing in an experimental rabbit model

10.21203/rs.2.16349/v1 ◽

2019 ◽

Author(s):

xiang Li ◽

Ruimin Xie ◽

xiong Wang ◽

bohua Li ◽

Yongping Wang

Keyword(s):

Hyaluronic Acid ◽

Fracture Healing ◽

Cell Cultures ◽

Rabbit Model ◽

Bone Cell ◽

Mineral Density ◽

Biological Responses ◽

Related Transcription Factor ◽

Bone Cell Cultures

Abstract Background: Fracture healing is regulated by endocrine hormones and biochemical and biophysical factors, including hyaluronic acid (HA), which is a component of the extracellular matrix. The prerequisites for biological responses that promote fracture healing, such as biomechanical conditions, and molecular factors, can be investigated in bone cell cultures. Methods: In this study, HA promoted the formation of calcium nodules and the expression of runt-related transcription factor (Runx)2 and osteocalcin (OCN) proteins in MC3T3-E1 cells. Results: In a rabbit tibial fracture model, HA promoted formation of larger calluses than those in control or sham-treated animals at 2, 4, 6, and 8 weeks (P < 0.05). Osteophytes were larger in HA-treated than in control animals at 4 and 6 weeks (P < 0.05). Bone mineral density was greater in HA-treated than in control animals at 4, 6, and 8 weeks (P < 0.05). Conclusions: The results showed that HA promoted the formation of calcified nodules in bone cell cultures and healing of rabbit tibial fractures.

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