Molecular genetic manipulation of Pichia pastoris SEC4 governs cell growth and glucoamylase secretion

2005 ◽  
Vol 336 (4) ◽  
pp. 1172-1180 ◽  
Author(s):  
Shi-Hwei Liu ◽  
Wei-I Chou ◽  
Shu-Chuan Lin ◽  
Chia-Chin Sheu ◽  
Margaret Dah-Tsyr Chang
Keyword(s):  
Cell Growth ◽  
Pichia Pastoris ◽  
Genetic Manipulation ◽  
Molecular Genetic

New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris

Gene ◽  
2001 ◽  
Vol 263 (1-2) ◽  
pp. 159-169 ◽  
Author(s):  
Geoffrey P. Lin Cereghino ◽  
Joan Lin Cereghino ◽  
Anthony Jay Sunga ◽  
Monique A. Johnson ◽  
May Lim ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Selectable Marker ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Host Strain

scholarly journals Analysis of Codon Usage Patterns in Giardia duodenalis Based on Transcriptome Data from GiardiaDB

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1169
Author(s):  
Xin Li ◽  
Xiaocen Wang ◽  
Pengtao Gong ◽  
Nan Zhang ◽  
Xichen Zhang ◽  
...  
Keyword(s):  
Codon Usage ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Gc Content ◽  
Giardia Duodenalis ◽  
Codon Usage Pattern ◽  
Protein Size ◽  
New Genes ◽  
Optimal Codons ◽  
Usage Patterns

Giardia duodenalis, a flagellated parasitic protozoan, the most common cause of parasite-induced diarrheal diseases worldwide. Codon usage bias (CUB) is an important evolutionary character in most species. However, G. duodenalis CUB remains unclear. Thus, this study analyzes codon usage patterns to assess the restriction factors and obtain useful information in shaping G. duodenalis CUB. The neutrality analysis result indicates that G. duodenalis has a wide GC3 distribution, which significantly correlates with GC12. ENC-plot result—suggesting that most genes were close to the expected curve with only a few strayed away points. This indicates that mutational pressure and natural selection played an important role in the development of CUB. The Parity Rule 2 plot (PR2) result demonstrates that the usage of GC and AT was out of proportion. Interestingly, we identified 26 optimal codons in the G. duodenalis genome, ending with G or C. In addition, GC content, gene expression, and protein size also influence G. duodenalis CUB formation. This study systematically analyzes G. duodenalis codon usage pattern and clarifies the mechanisms of G. duodenalis CUB. These results will be very useful to identify new genes, molecular genetic manipulation, and study of G. duodenalis evolution.

scholarly journals The Plastid of Toxoplasma gondii Is Divided by Association with the Centrosomes

2000 ◽  
Vol 151 (7) ◽  
pp. 1423-1434 ◽  
Author(s):  
Boris Striepen ◽  
Michael J. Crawford ◽  
Michael K. Shaw ◽  
Lewis G. Tilney ◽  
Frank Seeber ◽  
...  
Keyword(s):  
Cell Division ◽  
Toxoplasma Gondii ◽  
Genetic Manipulation ◽  
Fluorescent Proteins ◽  
Recombinant Expression ◽  
Molecular Genetic ◽  
Time Lapse ◽  
Microtubule Organization ◽  
Apicomplexan Parasites ◽  
Daughter Cells

Apicomplexan parasites harbor a single nonphotosynthetic plastid, the apicoplast, which is essential for parasite survival. Exploiting Toxoplasma gondii as an accessible system for cell biological analysis and molecular genetic manipulation, we have studied how these parasites ensure that the plastid and its 35-kb circular genome are faithfully segregated during cell division. Parasite organelles were labeled by recombinant expression of fluorescent proteins targeted to the plastid and the nucleus, and time-lapse video microscopy was used to image labeled organelles throughout the cell cycle. Apicoplast division is tightly associated with nuclear and cell division and is characterized by an elongated, dumbbell-shaped intermediate. The plastid genome is divided early in this process, associating with the ends of the elongated organelle. A centrin-specific antibody demonstrates that the ends of dividing apicoplast are closely linked to the centrosomes. Treatment with dinitroaniline herbicides (which disrupt microtubule organization) leads to the formation of multiple spindles and large reticulate plastids studded with centrosomes. The mitotic spindle and the pellicle of the forming daughter cells appear to generate the force required for apicoplast division in Toxoplasma gondii. These observations are discussed in the context of autonomous and FtsZ-dependent division of plastids in plants and algae.

scholarly journals The Expanding Molecular Genetics Tool Kit in Chlamydia

2018 ◽  
Vol 200 (24) ◽  
Author(s):  
Raphael H. Valdivia ◽  
Robert J. Bastidas
Keyword(s):  
Genetic Engineering ◽  
Molecular Genetics ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Model System ◽  
New Method ◽  
Content Type ◽  
Host Interactions ◽  
Additional Tool ◽  
Genomic Regions

ABSTRACT Chlamydia has emerged as an important model system for the study of host pathogen interactions, in part due to a resurgence in the development of tools for its molecular genetic manipulation. An additional tool, published by Keb et al. (G. Keb, R. Hayman, and K. A. Fields, J. Bacteriol. 200:e00479-18, 2018, https://doi.org/10.1128/JB.00479-18), now allows for custom genetic engineering of genomic regions that were traditionally recalcitrant to genetic manipulation, such as genes within operons. This new method will be an essential instrument for the elucidation of Chlamydia-host interactions.

Molecular Genetic Manipulation of Mosquito Vectors

1995 ◽  
Vol 40 (1) ◽  
pp. 359-388 ◽  
Author(s):  
J Carlson ◽  
K Olson ◽  
S Higgs ◽  
B Beaty
Keyword(s):  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Mosquito Vectors

scholarly journals Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1) Regulation

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0167766 ◽  
Author(s):  
Wei Shen ◽  
Chuixing Kong ◽  
Ying Xue ◽  
Yiqi Liu ◽  
Menghao Cai ◽  
...  
Keyword(s):  
Cell Growth ◽  
Pichia Pastoris ◽  
Alcohol Oxidase ◽  
Kinase Screening

scholarly journals Golgi Structure Correlates with Transitional Endoplasmic Reticulum Organization in Pichia pastoris and Saccharomyces cerevisiae

1999 ◽  
Vol 145 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Olivia W. Rossanese ◽  
Jon Soderholm ◽  
Brooke J. Bevis ◽  
Irina B. Sears ◽  
James O'Connor ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Fluorescent Protein ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Coat Proteins ◽  
Transport Vesicles ◽  
Copii Vesicle ◽  
Copii Vesicles ◽  
Green Fluorescent

Golgi stacks are often located near sites of “transitional ER” (tER), where COPII transport vesicles are produced. This juxtaposition may indicate that Golgi cisternae form at tER sites. To explore this idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharomyces cerevisiae, which has a dispersed Golgi. tER structures in the two yeasts were visualized using fusions between green fluorescent protein and COPII coat proteins. We also determined the localization of Sec12p, an ER membrane protein that initiates the COPII vesicle assembly pathway. In P. pastoris, Golgi stacks are adjacent to discrete tER sites that contain COPII coat proteins as well as Sec12p. This arrangement of the tER-Golgi system is independent of microtubules. In S. cerevisiae, COPII vesicles appear to be present throughout the cytoplasm and Sec12p is distributed throughout the ER, indicating that COPII vesicles bud from the entire ER network. We propose that P. pastoris has discrete tER sites and therefore generates coherent Golgi stacks, whereas S. cerevisiae has a delocalized tER and therefore generates a dispersed Golgi. These findings open the way for a molecular genetic analysis of tER sites.

Prospects for the molecular genetic manipulation of livestock

1992 ◽  
Vol 1992 ◽  
pp. 7-7
Author(s):  
Ian Wilmut
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Target Population ◽  
Early Embryo ◽  
Animal Production ◽  
Farm Animals ◽  
Effective Scheme ◽  
Pharmaceutical Proteins ◽  
Repair Mechanisms

Molecular genetic manipulation can now be used to produce pharmaceutical proteins in the milk of farm animals. In the longer term this technology will be used to modify aspects of animal production, but it is not clear how many manipulations will be useful nor when such applications will become practicable. Improvements are required in all four aspects of an effective scheme for either gene transfer or targeting: it must be possible 1). to make the change, 2). to regulate expression of the gene in the desired manner, 3). to identify genes that are able to have a significant effect and 4). to disseminate the change into the target population.There is only one method that has been used to transfer a gene in livestock and this depends upon injection of a few hundred copies of the gene into a nucleus in the early embryo. It is believed that integration occurs because the act of injecting fluid causes breakages in chromosomes and that the repair mechanisms sometimes include injected DNA. There are serious implications.

Sign in / Sign up

Export Citation Format

Share Document