Electrochemical monitoring of Acidithiobacillus thiooxidans biofilm formation on graphite surface with elemental sulfur

2019 ◽  
Vol 128 ◽  
pp. 30-38 ◽  
Author(s):  
Marcela Méndez-Tovar ◽  
J. Viridiana García-Meza ◽  
Ignacio González
Keyword(s):  
Biofilm Formation ◽  
Elemental Sulfur ◽  
Graphite Surface ◽  
Acidithiobacillus Thiooxidans ◽  
Electrochemical Monitoring

Effect of Galactose on EPS Production and Attachment of Acidithiobacillus thiooxidans to Mineral Surfaces

2017 ◽  
Vol 262 ◽  
pp. 476-481
Author(s):  
Paulina Aguirre ◽  
Aminael Sánchez Rodríguez ◽  
Juan Carlos Gentina ◽  
Axel Schippers
Keyword(s):  
Biofilm Formation ◽  
Microbial Growth ◽  
Extracellular Polymeric Substances ◽  
Elemental Sulfur ◽  
Culture Media ◽  
Acidithiobacillus Thiooxidans ◽  
Mineral Surfaces ◽  
Planktonic Cells ◽  
Cell Adherence ◽  
A Cell

The presence of extracellular polymeric substances (EPS) and their relevance for biofilm formation on the mineral surface for a variety of microbial species play a fundamental role in the degradation of sulfide ores. EPS production is associated with induction or auto induction mechanisms as a response of bacteria to environmental conditions. In this study, we tested galactose as an inducer of EPS production in planktonic cells of Acidithiobacillus thiooxidans DSM 14887T and their adherence to polymetallic mineral surfaces. Cells of At. thiooxidans were first adapted to grow at different concentrations of galactose (0.15, 0.25, 0.35%) using a modified 9K liquid medium and elemental sulfur as the energy source. In order to determine EPS production, the microorganisms were grown for 24 hours at different concentrations of galactose. Our results showed a cell adherence of 84% cells within 4 hours in presence of 0.15% galactose compared to 70% without galactose. The optimal concentration of galactose for maximal EPS production was 0.25% and for the attachment of cells it was 0.15%. Higher galactose concentrations inhibited microbial growth and decreased the number of cells attached to the mineral. While with a small amount of galactose in the culture media can shift the balance between sessile cells and planktonic cells, generating an increase in adhesion and therefore a possible increase of the bioleaching rate.

Electrochemical Monitoring of Metallic Materials' Surfaces with Biofilm Formation

2020 ◽  
Vol MA2020-01 (44) ◽  
pp. 2525-2525
Author(s):  
Hideyuki Kanematsu ◽  
Reo Itoh ◽  
Sho Ogaki ◽  
Nobumitsu Hirai ◽  
Takeshi Kogo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Metallic Materials ◽  
Electrochemical Monitoring

scholarly journals An Optical Microbial Biosensor Based on Whole Cell of Acidithiobacillus thiooxidans for Hydrogen Sulfide Determination

2018 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Odemar Cardoso Silva ◽  
Andréa Medeiros Salgado ◽  
Francisca Pessoa De França
Keyword(s):  
Hydrogen Sulfide ◽  
Elemental Sulfur ◽  
Low Cost ◽  
Acidithiobacillus Thiooxidans ◽  
Bacterial Cells ◽  
Low Oxygen ◽  
Microbial Biosensor ◽  
Biosensor Application ◽  
Good Repeatability ◽  
Low Oxygen Concentration

Due to its corrosive and highly toxic character, the generation of hydrogen sulfide is a serious problem for the environment, human health, and the industry. This paper reported a new and simple methodology for aqueous hydrogen sulfide determination through the development of an optical microbial biosensor. The principle of detection was based on the aerobic and chemolithotrophic metabolism of Acidithiobacillus thiooxidans bacterial cells. Under low oxygen concentration and acidic conditions A. thiooxidans can rapidly oxidize H2S to elemental sulfur. The biochemical formation of elemental sulfur can be spectrophotometrically detected and the increase in absorbance at 620 nm exhibited a linear relationship to an H2S concentration up to 100 mg.L-1. The parameters concerning the analytical performance of the biosensor such as cell harvesting time and pH influence were measured and optimized through the optical absorption value. The biosensor was selective to H2S with no important disturbance by tested species except thiosulfate ion (11.5% error). Biosensor response expressed good repeatability (RSD = 4.46 %) and reproducibility (RSD = 4.66 %). The low cost of cell cultivation and the absence of the immobilization step make feasible the optic biosensor application.

Bioleaching of Orpiment (As2S3) in Absence of Fe3+

2015 ◽  
Vol 1130 ◽  
pp. 363-366
Author(s):  
Guang Ji Zhang ◽  
Qiao Yang ◽  
Chao Yang
Keyword(s):  
Elemental Sulfur ◽  
Sulfide Minerals ◽  
Experimental Results ◽  
Acidithiobacillus Thiooxidans ◽  
Ferric Ion ◽  
Arsenic Sulfide ◽  
Iron Ions ◽  
Mining Tailings ◽  
Planktonic Bacteria

Abstract Orpiment (As2S3) is one of the major arsenic sulfide minerals, which can be dissolved by ferric ion and proton produced by the bacteria, and the main intermediates were polysulfides and elemental sulfur. In this study, two strains (Acidithiobacillus thiooxidans and Acidthiobacillus caldus) were used to bioleach the orpiment without iron ions addition. The experimental results show that both the two strains can grow in the pulp and dissolve the orpiment. In the bioleaching, it was found that pH decreased, the concentration of arsenic increased significantly and no elemental sulfur is detected on the surface of the residuals. At the same time the density of the planktonic bacteria increased obviously. These results indicate that the orpiment can be dissolved by the bacteria easily in absence of Fe3+. This study suggests that the release of arsenic which is from orpiment in the mining tailings can be faster than expected in open air.

Bioleaching of Copper Slag Material

2017 ◽  
Vol 262 ◽  
pp. 61-64 ◽  
Author(s):  
Axel Schippers
Keyword(s):  
Elemental Sulfur ◽  
Copper Slag ◽  
Economic Feasibility ◽  
Copper Recovery ◽  
Processing Route ◽  
Low Grade ◽  
Acidithiobacillus Thiooxidans ◽  
Biological Assays ◽  
Copper Release ◽  
Mineral Phases

Bioleaching is applied mainly for copper recovery from low-grade sulfide ores via heap leaching. The main copper processing route includes pyrometallurgy and the remaining copper slag from smelting may still contain copper in amounts found in the ore. Here bioleaching of copper slag material with a copper content of about 1 % (grain size < 63 µm) and fayalite (Fe2SiO4) und magnetite (Fe3O4) as main mineral phases was tested in aerobic shake flask experiments with a mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidiphilium spp..To additionally test for reductive bioleaching, experiments under anaerobic conditions (80% N2, 20% CO2, v/v) with or without addition of elemental sulfur were run. The pH was adjusted to < 3 by addition of sulfuric acid. After the incubation period of more than 50 days at 30°C cell growth was observed in all biological assays. The redox potential was above 800 mV SHE in the aerobic biological assays and dropped to around 500 mV in the chemical control assays as well as in the anaerobic biological and chemical assays. A significant copper bioleaching was observed in the aerobic experiments with 91 % copper release (max. 35 % in the chemical controls). Anaerobic bioleaching experiments did not show a significant copper release, however the release of iron (as iron(II)) and sulfate was much higher than in the abiotic assays and several fold higher than in the classical aerobic bioleaching experiments. Overall the results show that copper bioleaching from slag material is possible, however the economic feasibility needs to be demonstrated.

scholarly journals Performance of Urea-Based Fertilizers Associated With Elemental Sulfur or Polymers on Ammonia Volatilization

2021 ◽  
Vol 13 (4) ◽  
pp. 73
Author(s):  
Odirley Rodrigues Campos ◽  
Edson Marcio Mattiello ◽  
Wedisson Oliveira Santos ◽  
Reinaldo Bertola Cantarutti ◽  
Rafael Coca Cuesta ◽  
...  
Keyword(s):  
Soil Ph ◽  
Ammonia Volatilization ◽  
Elemental Sulfur ◽  
Soil Surface ◽  
Acidithiobacillus Thiooxidans ◽  
Nh3 Volatilization ◽  
Oxidation Rates ◽  
Coated Urea ◽  
Polymer Coated Urea ◽  
Urea Granules

High N-NH3 losses are expected when conventional urea is applied to the soil surface. In order to reduce it, urea granules could be coated with different materials to decrease fertilizer dissolution rate or to stabilize N-NH4+ by acidification. In this study, we investigated the effect of a polymer-coated urea and powdered S0 added to urea, in the presence or absence of a S-oxidizing bacterium (Acidithiobacillus thiooxidans), on soil pH, SO42- availability, NH4+, and NH3 volatilization. Applying S0 before urea and the inoculation with bacteria have promoted the highest S0 oxidation rates. The greater decrease in soil pH occurred when S0 was applied before urea at a higher dose, which also decreased NH3 volatilization by 83% up to 4 days after urea application. However, the decrease in soil pH did not increase the concentration of NH4+, nor did it decrease the accumulated amount of volatilized NH3 over time. The inoculation of A. thiooxidans accelerates S0 oxidation process, but it was insufficient to counteract the H+ consumption by urea hydrolysis. Therefore, the S0 application with urea did not offer chemical protection against NH3 loss, but a physical barrier in the controlled-release urea had less dissolved urea in soil and reduced NH3 losses.

scholarly journals Nutrient availability in phosphate and potassic rocks induced by Acidithiobacillus oxidizing bacteria to produce biofertilizers

2020 ◽  
pp. 250-258
Author(s):  
Emmanuella Vila Nova da Silva ◽  
Newton Pereira Stamford ◽  
Wagner da Silva Oliveira ◽  
Valdomiro Severino de Souza Júnior ◽  
Lusiene Barbosa Sousa ◽  
...  
Keyword(s):  
Nutrient Availability ◽  
Elemental Sulfur ◽  
Initial Time ◽  
Acidithiobacillus Thiooxidans ◽  
Mineralogical Analysis ◽  
Sulfur Bacteria ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Set Up ◽  
Potassic Rocks

Production of conventional fertilizers requires significant energy consumption which increases the price of the product. In recent years, a growing interest on the application of natural products has been shown by public. The aim of this work is to evaluate the effectiveness of the sulfur bacteria Acidithiobacillus thiooxidans to promote mineralogy changes and increase nutrient availability, using phosphate and potassic rocks to produce PK biofertilizers. The study was set up in Petri dishes using 30 g of phosphate rock + 30 g of potassium rock, each rock mixed with elemental sulfur applied at two levels 10% and 20% (3 g and 6 g) in dish. The experiment was conducted in a completely randomized design with eight replications (each rock). The bacterium was inoculated applying 1 ml per g of elemental sulfur. The rocks were incubated during 180 days and mineralogical analysis by XRD and chemical analysis were processed at 30, 60, 120, 150 and 180 days after inoculation. The Acidithiobacillus produced sulfuric acid, which promoted mineralogical changes especially at 180 days after inoculation, compared with the initial time. The inoculation of Acidithiobacillus reduced pH and promoted mineralogical changes in P and K rocks with a significant increase in P and K availability, becoming a viable alternative to plant nutrition instead of soluble PK fertilizers. In addition, it showed great potential for a sustainable agriculture system.

scholarly journals Biofilm Formation by the Acidophile Bacterium Acidithiobacillus thiooxidans Involves c-di-GMP Pathway and Pel exopolysaccharide

Genes ◽  
2018 ◽  
Vol 9 (2) ◽  
pp. 113 ◽  
Author(s):  
Mauricio Díaz ◽  
Matias Castro ◽  
Sylvia Copaja ◽  
Nicolas Guiliani
Keyword(s):  
Biofilm Formation ◽  
Acidithiobacillus Thiooxidans
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