Nutrient availability in phosphate and potassic rocks induced by Acidithiobacillus oxidizing bacteria to produce biofertilizers

Production of conventional fertilizers requires significant energy consumption which increases the price of the product. In recent years, a growing interest on the application of natural products has been shown by public. The aim of this work is to evaluate the effectiveness of the sulfur bacteria Acidithiobacillus thiooxidans to promote mineralogy changes and increase nutrient availability, using phosphate and potassic rocks to produce PK biofertilizers. The study was set up in Petri dishes using 30 g of phosphate rock + 30 g of potassium rock, each rock mixed with elemental sulfur applied at two levels 10% and 20% (3 g and 6 g) in dish. The experiment was conducted in a completely randomized design with eight replications (each rock). The bacterium was inoculated applying 1 ml per g of elemental sulfur. The rocks were incubated during 180 days and mineralogical analysis by XRD and chemical analysis were processed at 30, 60, 120, 150 and 180 days after inoculation. The Acidithiobacillus produced sulfuric acid, which promoted mineralogical changes especially at 180 days after inoculation, compared with the initial time. The inoculation of Acidithiobacillus reduced pH and promoted mineralogical changes in P and K rocks with a significant increase in P and K availability, becoming a viable alternative to plant nutrition instead of soluble PK fertilizers. In addition, it showed great potential for a sustainable agriculture system.

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What is the digestibility and caloric value of different botanical parts in corn residue to cattle?1

Journal of Animal Science ◽

10.1093/jas/skz137 ◽

2019 ◽

Vol 97 (7) ◽

pp. 3056-3070 ◽

Cited By ~ 2

Author(s):

Emily A Petzel ◽

Evan C Titgemeyer ◽

Alexander J Smart ◽

Kristin E Hales ◽

Andrew P Foote ◽

...

Keyword(s):

Organic Matter ◽

Nutrient Availability ◽

Heat Production ◽

Latin Square ◽

Metabolizable Energy ◽

Net Energy ◽

Digestible Energy ◽

Randomized Design ◽

Corn Residue ◽

Completely Randomized Design

AbstractTwo experiments were conducted to measure rates of ruminal disappearance, and energy and nutrient availability and N balance among cows fed corn husks, leaves, or stalks. Ruminal disappearance was estimated after incubation of polyester bags containing husks, leaves or stalks in 2 separate ruminally cannulated cows in a completely randomized design. Organic matter (OM) that initially disappeared was greatest for stalks and least for husks and leaves (P < 0.01), but amounts of NDF that initially disappeared was greatest for husks, intermediate for stalks, and least for leaves (P < 0.01). Amounts of DM and OM that slowly disappeared were greatest in husks, intermediate in leaves, and least in stalks (P < 0.01). However, amounts of NDF that slowly disappeared were greatest in leaves, intermediate in husks, and least in stalks (P < 0.01). Rate of DM and OM disappearance was greater for leaves, intermediate for husks and least for stalks, but rate of NDF disappearance was greatest for stalks, intermediate for leaves, and least for husks (P < 0.01). Energy and nutrient availability in husks, leaves, or stalks were measured by feeding ruminally cannulated cows husk-, leaf-, or stalk-based diets in a replicated Latin square. Digestible energy lost as methane was less (P = 0.02) when cows were fed leaves in comparison to husks or stalks, and metabolizable energy (Mcal/kg DM) was greater (P = 0.03) when cows were fed husks and leaves compared with stalks. Heat production (Mcal/d) was not different (P = 0.74) between husks, leaves, or stalks; however, amounts of heat produced as a proportion of digestible energy intake were less (P = 0.05) among cows fed leaves in comparison to stalks or husks. Subsequently, there was a tendency (P = 0.06) for net energy available for maintenance from leaves (1.42 Mcal/kg DM) to be greater than stalks (0.91 Mcal/kg DM), and husks (1.30 Mcal/kg DM) were intermediate. Nitrogen balance was greater when cows were fed leaves, intermediate for husks, and least for stalks (P = 0.01). Total tract digestion of NDF was greater (P < 0.01) for husks and leaves compared with stalks. Husks had greater (P = 0.04) OM digestibility in comparison to stalks, and leaves were intermediate. Apparently, greater production of methane from husks in comparison to leaves limited amounts of energy available for maintenance from husks even though total-tract nutrient digestion was greatest when cows were fed husks or leaves.

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Managment of sulphur fertilizer in forage sorghum crop cultivated in eutrophic Cambisol with alkaline pH

Australian Journal of Crop Science ◽

10.21475/ajcs.19.13.08.p1530 ◽

2019 ◽

pp. 1258-1266 ◽

Cited By ~ 1

Author(s):

Thiago Henrique Ferreira Matos Castañon ◽

Boanerges Freire de Aquino ◽

Edna Maria Bonfim Silva ◽

Izabel Maria Almeida Lima ◽

Ana Paula Alves Barreto Damasceno

Keyword(s):

Elemental Sulfur ◽

Chemical Properties ◽

Dry Mass ◽

Alkaline Ph ◽

Single Superphosphate ◽

Soil Fertilization ◽

Forage Sorghum ◽

Randomized Design ◽

Sulphur Fertilizer ◽

Completely Randomized Design

The objective of this study was to analyze the effect of soil fertilization with sulfur-based fertilizers, sulfate and elemental sulfur forms on biomass production, nutrient characteristics of sorghum and soil chemical properties. The experiment was carried out in a 4 x 4 factorial scheme (four sulfur sources: single superphosphate, agricultural gypsum, elemental sulfur powder and elemental sulfur granulated with bentonite, and four sulfur doses: 0, 40, 80, 120 mgdm-3) using four replications in a completely randomized design, being cultivated in pots under greenhouse conditions. The sorghum was cultivated for a period of 51 days after emergence of the seedlings. The shoot dry mass, shoot macronutrients content, root and soil and pH of the soil were evaluated. There were interactions between sources and sulfur doses in the variables such as shoot dry mass, sulfur in the root, sulfur and calcium in the soil. Elemental sulfur (granulate) showed lower concentrations of phosphorus, sulfur and N:S ratio in the shoot. The concentrations of potassium, calcium and magnesium did not show significant differences, both for the shoot and the root. The pH of the soil was reduced depending on the sources and doses of elemental sulfur. The sources and doses of sulfur did not influence the levels of phosphorus, potassium, and magnesium in the soil. The elemental sulfur in the form of powder is the best source of sulfur for forage sorghum cultivated in soil with alkaline pH.

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Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.08.p2240 ◽

2020 ◽

pp. 1236-1241

Author(s):

Gustavo H. Sera ◽

Filipe G. Carvalho ◽

Inês C. de B. Fonseca ◽

Luciana H. Shigueoka ◽

Santino A. da Silva ◽

...

Keyword(s):

Germplasm Bank ◽

Breeding Programs ◽

Arabica Coffee ◽

Randomized Design ◽

Mother Plants ◽

Completely Randomized Design ◽

Set Up ◽

Moderately Resistant ◽

Reproduction Factor ◽

Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

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OCDs in Completely Randomized Design Set-Up

Optimal Covariate Designs ◽

10.1007/978-81-322-2461-7_2 ◽

2015 ◽

pp. 13-26

Author(s):

Premadhis Das ◽

Ganesh Dutta ◽

Nripes Kumar Mandal ◽

Bikas Kumar Sinha

Keyword(s):

Randomized Design ◽

Completely Randomized Design ◽

Set Up

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In vitro development of Encyclea cordigera in different concentrations of honey

Ciência Rural ◽

10.1590/0103-8478cr20150046 ◽

2015 ◽

Vol 46 (4) ◽

pp. 590-592 ◽

Cited By ~ 2

Author(s):

Cibele Mantovani ◽

Kathia Fernandes Lopes Pivetta

Keyword(s):

Culture Media ◽

Leaf Length ◽

In Vitro Development ◽

Randomized Design ◽

Sucrose Medium ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Set Up ◽

Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

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Trichoderma Contributes to the Germination and Seedling Development of Açaí Palm

Agriculture ◽

10.3390/agriculture10100456 ◽

2020 ◽

Vol 10 (10) ◽

pp. 456

Author(s):

Bruno Fróes Campos ◽

Anselmo Junior Corrêa Araújo ◽

Cristina Aledi Felsemburgh ◽

Thiago Almeida Vieira ◽

Denise Castro Lustosa

Keyword(s):

Dry Mass ◽

Initial Growth ◽

Randomized Design ◽

Trichoderma Isolate ◽

Number Of Leaves ◽

Collar Diameter ◽

Completely Randomized Design ◽

Set Up ◽

Root Size ◽

Trichoderma Isolates

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.

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Penggunaan Pupuk Organik Cair Sebagai Media Produksi Inokulan Azotobacter Chroococcum

Agrologia ◽

10.30598/a.v2i2.264 ◽

2018 ◽

Vol 2 (2) ◽

Author(s):

Reginawanti Hindersah ◽

H Yulina ◽

Ane Nurbaity

Keyword(s):

Organic Fertilizer ◽

Scaling Up ◽

Azotobacter Chroococcum ◽

Growth Media ◽

Total N ◽

Support Cell ◽

Commercial Scale ◽

Randomized Design ◽

Completely Randomized Design ◽

Set Up

Azotobacter chroococcum based biofertilizers have been widely used in the production of environmentally agriculture. For commercial scale, cheap growth media for Azotobcter inoculant will affect the quality and price of liquid biofertilizer. Growth medium should support cell proliferation as well as its nitrogen fixation and phytohormone production. The objective of this study was to determine whether liquid organic fertilizer (LOF) containing low N can be used as a medium for scaling up A. chroococcum inoculant without lowering the survival of bacteria and its activity in N2 fixation and phytohormones production. LOF generally contains a complete nutrient although in a small quantities. Experiment was set up in completely randomized design which tested two types of commercial LOF. The results showed that both of LOF were only able to support cell growth up to 72 hours and at 96 hours cell viability began to decline compared to the control medium, free-N Ashby. At 72 hour total N and cytokinin concentration in LOF was lower than those in Ashby media. This study revealed that LOF could be used as A. chroococcum growth media, but without optimization of its composition, cell density and cytokinin content would be lower than those of inoculant in Ashby Media.

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Keanekaragaman Dan Kemerataan Serangga Pada Areal Tanaman Kentang (Solanum tuberosum L) Setelah Berbagai Metode Aplikasi Insektisida

Agrologia ◽

10.30598/a.v4i1.218 ◽

2015 ◽

Vol 4 (1) ◽

Author(s):

Evawani Tomayahu

Keyword(s):

Solanum Tuberosum L ◽

Economic Value ◽

Insect Population ◽

Limiting Factors ◽

Control Treatment ◽

Pesticide Application ◽

Insecticide Application ◽

Randomized Design ◽

Completely Randomized Design ◽

Set Up

Pest is one of the biological limiting factors that led to low production of potatoes. The use of pesticides is a solution in controlling pests. The objective of this study was to determine the diversity and evenness of insect; and potato yield losses due to insecticide applications methods. The treatment was controlled and intensive insecticide application which were compared with no insecticide application. The experiment was set up in completely randomized design with five replications. The results showed that the insects are dominated by Empoasca sp, Liriomyza sp and Miridae. The highest insect population was Empoasca sp. i.e 80.83 Empoasca sp. which was found in control treatment. Controlled and intensive application of insecticides decreased insect population up to 56.50 and 26.17. Evenness index of insect and economic value of lost results unaffected by differences in pesticide application methods

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Elemental sulfur recommendation for pH reduction in soils from Southern Brazil

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v26n3p212-218 ◽

2022 ◽

Vol 26 (3) ◽

pp. 212-218

Author(s):

Daniel J. Dall’Orsoletta ◽

Gilmar L. Mumbach ◽

Fernando M. Brignoli ◽

Luciano C. Gatiboni

Keyword(s):

Soil Ph ◽

Elemental Sulfur ◽

Southern Brazil ◽

Soil Variables ◽

Randomized Design ◽

Ph Reduction ◽

Aluminum Saturation ◽

Completely Randomized Design ◽

Potential Acidity ◽

Low Rate

ABSTRACT The elemental sulfur (S0) application may reduce soil pH, benefiting plants adapted to acid conditions and lessening problems of overliming. Nevertheless, there is no official recommendation for its application. The objective of the study was to quantify the S0 doses required to reduce the pH of soils from Southern Brazil. The experiment was carried out in the laboratory in a factorial scheme (5 × 5), with a completely randomized design and three replicates. The treatments consisted of five soils, and five doses of S0, corresponding to 0, 50, 100, 150, and 200% of the estimated dose need to reach pH 4.0. The applied doses of S0 resulted in reduction of pH and base saturation (V%) and increase of potential acidity (H + Al). These effects, however, were reduced due to the low rate of oxidation of the S0 applied (0.76-3.36%). The soil variables correlated with S0 oxidation were Mg2+ (0.86***), Al3+ (-0.82***), H + Al (-0.89***), V% (0.68***) and aluminum saturation (m%) (-0.87***). In the evaluated soils the oxidation of 50 kg ha-1 of S0 was required to reduce one unit of pH in H2O.

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Stability of soybean yield through different sowing periods

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2000001100009 ◽

2000 ◽

Vol 35 (11) ◽

pp. 2181-2189 ◽

Cited By ~ 6

Author(s):

WILMAR FERREIRA LIMA ◽

JOSÉ FRANCISCO FERRAZ DE TOLEDO ◽

CARLOS ALBERTO ARRABAL ARIAS ◽

MARCELO FERNANDES DE OLIVEIRA

Keyword(s):

Minimum Variance ◽

Soybean Yield ◽

Latitude Range ◽

Productivity Losses ◽

Randomized Design ◽

Supplementary Irrigation ◽

Completely Randomized Design ◽

Set Up ◽

Sowing Period ◽

Hill Plots

Soybean yield is highly affected by sowing period and there are significant productivity losses when sowings are done outward a relatively restricted period in many regions of Brazil. Breeding cultivars less sensitive to photoperiod and to temperature variations is desirable for adaptation to wider sowing period and wider latitude range and also make irrigated soybean cultivation possible during the fall-winter seasons in frost free regions. The possibility of selecting high yielding and stable lines for yield during various sowing periods was studied by analyzing the behavior of 100 non-selected advanced lines (F9 and F10), from each one of all possible biparental crosses involving the genotypes BR85-29009, OCEPAR 8, FT-2, and BR-13. Experiments were set up in a completely randomized design with single-plant hill plots and received supplementary irrigation. Sowing was on Sept 27, Oct 20, Nov 17, and Dec 17 in 1993/94 and Sept 20, Oct 20, Nov 17, and Dec 14 in 1994/95 at Londrina, PR, Brazil. Procedures of regression analysis and minimum variance among planting date means were efficient for selecting stable lines during the four sowing seasons. It was possible to select stable and high yielding genotypes through the four sowing periods in all the crosses. No specific cross was clearly better to produce a greater number of stable genotypes.

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