Synergistic anti-cancer effects of galangin and berberine through apoptosis induction and proliferation inhibition in oesophageal carcinoma cells

Introduction: Due to the side effects of drugs, the development of nanoscale drug delivery systems has led to a significant improvement in medicinal therapies due to drug pharmacokinetics changes, decreased toxicity, and increased half-life of the drug. This study aimed to synthesize tamoxifen (TMX)-loaded L-lysine coated magnetic iron oxide nanoparticles as a nano-carrier to investigate its cytotoxic effects and anti-cancer properties against MCF-7 cancer cells. Methods: Magnetic Fe3O4 nanoparticles were synthesized and coated with L-lysine (F-Lys NPs). Then, TMX was loaded onto these NPs. The characteristics of synthesized nanoparticles (F-Lys-TMX NPs) were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), differential scanning calorimetry (DSC), vibrating sample magnetometer (VSM), and thermogravimetric analysis (TGA). The drug release was analyzed at pH 5.8 and pH 7.4. The MCF-7 cells were exposed to F-Lys-TMX NPs, F-Lys NPs, and TMX for 24, 48, and 72 hours. To evaluate the cytotoxic potential of designed nanoparticles, MTT and apoptosis assays, real-time PCR, and cell cycle analysis was carried out. Results: The F-Lys-TMX NPs had spherical morphology with a size ranging from 9 to 30 nm. By increasing the nanoparticles concentration and treatment time, more cell proliferation inhibition and apoptosis induction were observed in F-Lys-TMX NPs-treated cells compared to the TMX. The expression levels of ERBB2, cyclin D1, and cyclin E genes were down-regulated and expression levels of the caspase-3 and caspase-9 genes were up-regulated. Studies on the drug release revealed a slow and controlled pH-dependent release of the nanoparticles. Cell cycle analysis indicated that F-Lys-TMX NPs could arrest the cells at the G0/G1 phase. Conclusion: The findings suggest that F-Lys-TMX NPs are more effective and have the potential for cell proliferation inhibition and apoptosis induction compared to the TMX. Hence, F-Lys-TMX NPs can be considered as an anti-cancer agent against MCF-7 breast cancer cells.

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Anti-Cancer Effects of Citalopram on Hepatocellular Carcinoma Cells Occur via Cytochrome C Release and the Activation of NF-kB

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170327155930 ◽

2017 ◽

Vol 17 (11) ◽

Cited By ~ 17

Author(s):

Elham Ahmadian ◽

Aziz Eftekhari ◽

Hossein Babaei ◽

Alireza M. Nayebi ◽

Mohammad A. Eghbal

Keyword(s):

Hepatocellular Carcinoma ◽

Cytochrome C ◽

Carcinoma Cells ◽

Cytochrome C Release ◽

Hepatocellular Carcinoma Cells ◽

Anti Cancer

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Cellular and Molecular Aspects of Anti-Melanoma Effect of Minocycline—A Study of Cytotoxicity and Apoptosis on Human Melanotic Melanoma Cells

International Journal of Molecular Sciences ◽

10.3390/ijms21186917 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6917

Author(s):

Jakub Rok ◽

Zuzanna Rzepka ◽

Artur Beberok ◽

Justyna Pawlik ◽

Dorota Wrześniok

Keyword(s):

Myeloid Leukemia ◽

Standard Treatment ◽

Annexin V ◽

Medical Problem ◽

Melanoma Cells ◽

Carcinoma Cells ◽

Anti Cancer ◽

Acute Myeloid Leukemia Cells ◽

Molecular Aspects ◽

Therapy Result

Minocycline is a tetracycline compound with pleiotropic pharmacological properties. In addition to its antibacterial action, it shows many non-antimicrobial effects, including an anti-cancer activity. The anti-cancer action was confirmed in studies on ovarian carcinoma cells, hepatocellular carcinoma cells, glioma cells, or acute myeloid leukemia cells. Malignant melanoma remains a serious medical problem despite the extensive knowledge of the disease. The low effectiveness of the standard treatment, as well as the resistance to therapy, result in high mortality rates. This work aimed to investigate the potential and mechanisms of anti-melanoma action of minocycline. Human skin melanotic melanoma cell line COLO 829 was used in the study. The obtained results showed that minocycline decreased cell viability and inhibited the growth of melanoma cells, proportional to the drug concentration as well as to the time of incubation. The EC50 values were calculated to be 78.6 µM, 31.7 µM, and 13.9 µM for 24 h, 48 h, and 72 h, respectively. It was observed that treated cells had a disturbed cell cycle and significantly changed morphology. Moreover, minocycline caused a decrease in mitochondrial membrane potential and an increase in cells with a low level of reduced thiols. Finally, it was found that the anti-melanoma effect of minocycline was related to the induction of apoptosis. The drug activated caspases 8, 9, and 3/7 as well as increased the number of annexin V-positive cells. The presented results show that minocycline possesses anti-melanoma potential.

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Growth inhibition and apoptosis induction of tanshinone II-A on human hepatocellular carcinoma cells

World Journal of Gastroenterology ◽

10.3748/wjg.v10.i14.2024 ◽

2004 ◽

Vol 10 (14) ◽

pp. 2024 ◽

Cited By ~ 91

Author(s):

Shu-Lan Yuan

Keyword(s):

Hepatocellular Carcinoma ◽

Growth Inhibition ◽

Carcinoma Cells ◽

Human Hepatocellular Carcinoma ◽

Apoptosis Induction ◽

Tanshinone Ii A ◽

Hepatocellular Carcinoma Cells ◽

Human Hepatocellular Carcinoma Cells

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Combination of SL327 and Sunitinib Malate leads to an additive anti-cancer effect in doxorubicin resistant thyroid carcinoma cells

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.01.135 ◽

2017 ◽

Vol 88 ◽

pp. 985-990 ◽

Cited By ~ 7

Author(s):

Wei Wang ◽

Jin Zhou ◽

Lujie Zhao ◽

Shulin Chen

Keyword(s):

Thyroid Carcinoma ◽

Carcinoma Cells ◽

Sunitinib Malate ◽

Anti Cancer

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Sulforaphane suppresses autophagy during the malignant progression of gastric carcinoma via activating miR-4521/PIK3R3 pathway

Human & Experimental Toxicology ◽

10.1177/09603271211054437 ◽

2021 ◽

pp. 096032712110544

Author(s):

Zi-tan Peng ◽

Pei Gu

Keyword(s):

Cell Proliferation ◽

Gastric Carcinoma ◽

Carcinoma Cells ◽

Malignant Progression ◽

Gastric Carcinoma Cell ◽

Over Expression ◽

Anti Cancer ◽

Underlying Mechanisms ◽

Cruciferous Plants

Objective Sulforaphane, which exerts an effective anti-cancer ability, is a phytochemical converted from cruciferous plants. Here, we aimed to identify whether sulforaphane could suppress autophagy during the malignant progression of gastric carcinoma and to explore the underlying mechanisms. Methods SGC7901 cells were transfected with miR-4521 mimics, inhibitor, and pcDNA3.1- PIK3R3, and treated with sulforaphane or autophagy inhibitor. Cell proliferation, apoptosis, and miR-4521 or PIK3R3 expression were detected. Results MiR-4521 over-expression suppressed LC3-II/I ratio and Beclin-1 expression but induced p62 expression in SGC7901 cells. MiR-4521 also reduced gastric carcinoma cell proliferation and promoted apoptosis in vitro. In the mechanical observation, we identified that miR-4521 directly targeted PIK3R3 to repress its expression, and PIK3R3 up-regulation partly antagonized miR-4521-mediated autophagy, proliferation, and apoptosis in gastric carcinoma cells. In addition, sulforaphane exerted effective anti-cancer functions by repressing autophagy and growth in tumor cells at a concentration-dependent way. MiR-4521 inhibition or PIK3R3 over-expression weakened the anti-cancer functions of sulforaphane in gastric carcinoma cells. Conclusion Consequently, miR-4521 suppressed autophagy during the malignant progression of gastric carcinoma by targeting PIK3R3. Thus, miR-4521 may be applied as a therapeutic target for sulforaphane in gastric carcinoma.

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