scholarly journals Total flavonoids of rhizoma drynariae ameliorates bone formation and mineralization in BMP-Smad signaling pathway induced large tibial defect rats

2021 ◽  
Vol 138 ◽  
pp. 111480
Author(s):  
Weipeng Sun ◽  
Minying Li ◽  
Yan Zhang ◽  
Yingjie Huang ◽  
Qunzhang Zhan ◽  
...  
Keyword(s):  
Bone Formation ◽  
Signaling Pathway ◽  
Total Flavonoids ◽  
Smad Signaling ◽  
Tibial Defect ◽  
Smad Signaling Pathway ◽  
Rhizoma Drynariae

scholarly journals Medium-Intensity Treadmill Exercise Exerts Beneficial Effects on Bone Modeling Through Bone Marrow Mesenchymal Stromal Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Lingli Zhang ◽  
Yu Yuan ◽  
Wei Wu ◽  
Zhongguang Sun ◽  
Le Lei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Signaling Pathway ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Treadmill Exercise ◽  
Heterotopic Bone ◽  
Smad Signaling ◽  
Medium Intensity ◽  
Smad Signaling Pathway

As a type of multipotential cells, bone marrow mesenchymal stromal cells (BMMSCs) can differentiate into chondrocytes, osteoblasts, and adipocytes under different loading condition or specific microenvironment. Previous studies have shown that BMMSCs and their lineage-differentiated progeny (for example, osteoblasts), and osteocytes are mechanosensitive in bone. The appropriate physical activity and exercise could help attenuate bone loss, effectively stimulate bone formation, increase bone mineral density (BMD), prevent the progression of osteoporosis, and reduce the risk of bone fractures. Bone morphogenetic protein (BMP) is originally discovered as a protein with heterotopic bone-inducing activity in the bone matrix that exerts a critical role in multiple stages of bone metabolism. In the present study, the medium-intensity treadmill exercise enhanced bone formation and increased osteocalcin (OCN) and osteopontin (OPN) mRNA expression as well as activation of the BMP-Smad signaling pathway in vivo. In order to investigate the effect of a BMP-Smad signaling pathway, we injected mice with activated enzyme inhibitors (LDN-193189HCL) and subjected the mice to treadmill exercise intervention. LDN-193189HCL attenuated the BMD and bone mass mediated by medium-intensity exercise and BMP-Smad signaling pathway.

Artesunate inhibits the development of PVR by suppressing the TGF-β/Smad signaling pathway

2021 ◽  
pp. 108859
Author(s):  
Zi-Yi Wang ◽  
Yu Zhang ◽  
Jie Chen ◽  
Ling-Dan Wu ◽  
Mei-Ling Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Smad Signaling ◽  
Smad Signaling Pathway

scholarly journals Retinoic acid receptors interfere with the TGF-β/Smad signaling pathway in a ligand-specific manner

Oncogene ◽  
2003 ◽  
Vol 22 (50) ◽  
pp. 8212-8220 ◽  
Author(s):  
Valérie Pendaries ◽  
Franck Verrecchia ◽  
Serge Michel ◽  
Alain Mauviel
Keyword(s):  
Retinoic Acid ◽  
Signaling Pathway ◽  
Retinoic Acid Receptors ◽  
Smad Signaling ◽  
Specific Manner ◽  
Smad Signaling Pathway

scholarly journals Periplaneta Americana Extract may Attenuate 2,4,6-Trinitrobenzenesulfonic Acid-Induced Intestinal Fibrosis by Inhibiting TGF-β/Smad Signaling Pathway

2021 ◽  
Author(s):  
Jing Liu ◽  
Pin Lv ◽  
Xiang Rao ◽  
Jiajia Wang
Keyword(s):  
Western Blot ◽  
Signaling Pathway ◽  
Real Time Pcr ◽  
Periplaneta Americana ◽  
Collagen I ◽  
Smad Signaling ◽  
Intestinal Fibrosis ◽  
Smad Signaling Pathway

Abstract PurposeIntestinal fibrosis is an incurable digestive disease accompanied by stricture formation, and it has an increasing incidence in recent years. Periplaneta americana is one of the medicinal insects with a long history. There are few reports on the effect of intestinal fibrosis. This study aims to evaluate the inhibitory effect of PA treatment on intestinal fibrosis. MethodsTNBS was used to establish intestinal fibrosis model by enema in BALB/c mice. The mice were treated with PA (50, 100, 200 mg/kg body weight) and 5-aminosalicylic acid (5-ASA) (40mg/kg) by gavage once a day for 6 weeks. At the end of the last week, the mice were sacrificed. Colon samples were collected for H&E and Masson staining. The mRNA and protein expression of α-smooth muscle actin (α-SMA), collagen I and the transforming growth factor-β (TGF-β) / Smad signaling pathway were conducted by real-time PCR and western blot analysis. In vitro, TGF-β1 was used to induce intestinal fibrosis at human colon fibroblasts (CCD-18Co). And using real-time PCR and western blot methods to detect the expression of α-SMA and collagen I. ResultsPA inhibited the expression of α-SMA and collagen I in vivo and in vitro. But the difference was that PA inhibited the TGF-β/Smad signaling pathway in vivo, and the same results had not been obtained in vitro. Conclusion: PA may attenuate intestinal fibrosis by inhibiting TGF-β/Smad signaling pathway, but more experiments were needed to prove it in vitro. ConclusionsPA has potential pharmacological effects in inhibiting intestinal fibrosis, and the TGF-β/Smad signaling pathway seemed promising.

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