Effect of Cellulase Enzymes on Degradation Characteristics of Ensiled Rice Straw

1988 ◽  
Vol 1988 ◽  
pp. 83-83
Author(s):  
Y. Nakashima ◽  
E.R. Ørskov ◽  
K. Ambo ◽  
Y. Takase
Keyword(s):  
Rice Straw ◽  
Dry Matter ◽  
Room Temperature ◽  
Degradation Rate ◽  
Enzyme Preparation ◽  
Lower Content ◽  
Cellulase Enzymes ◽  
Cellulase Enzyme ◽  
Physical Form ◽  
Degradation Rate Constant

AbstractRice straw was ensiled in laboratory containers of 11 capacity. Three concentrations of moisture (50, 60 or 70%) 3 concentrations of a commercial cellulase enzyme preparation (0, 5 or 10g/kg dry matter (DM)) and 3 types of straw processing (2 cm, 5 mm or 2 mm length) were used. The preparations were stored at room temperature (approximately 20°C) for 30 days. The straw treated with cellulase had a lower final pH (5.21, 4.87, 4.82; P<0.05), higher concentrations of lactic acid (198, 383, 367 mg/100g; P<0.05), a lower content of neutral detergent fibre (689, 630, 620 g/kg DM; P<0.05) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P<0.05) for the 0, 5 and 10 g/kg cellulase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of 3 sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p+a+b(1-e-ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was significantly increased (P<0.05) by the addition of cellulase. The maximum potential (a+b) however was unchanged. The values for c were 0.0496, 0.0677 and 0.0847/h-1 and the values for (a+b) were 624, 621 and 628 g/kg for the 0, 5 and 10 g/kg cellulase enzyme additions respectively. It is concluded that the use of cellulase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

Rumen degradation of straw 6. Effect of polysaccharidase enzymes on degradation characteristics of ensiled rice straw

1988 ◽  
Vol 47 (3) ◽  
pp. 421-427 ◽  
Author(s):  
Y. Nakashima ◽  
E. R. Ørskov ◽  
P. M. Hotten ◽  
K. Ambo ◽  
Y Takase
Keyword(s):  
Moisture Content ◽  
Rice Straw ◽  
Plant Material ◽  
Dry Matter ◽  
Degradation Rate ◽  
Lower Content ◽  
Rumen Degradation ◽  
Physical Form ◽  
Degradation Rate Constant ◽  
Inclusion Level

ABSTRACTThe effects of three variables; polysaccharidase inclusion level (0, 5 and 10 g/kg), moisture content (500, 600 and 700 g/kg dry matter (DM), and particle size (20, 5 and 2 mm), on the quality and degradation characteristics of ensiled rice straw were investigated. The polysaccharidase product used was shown to contain a broad spectrum of activities against polysaccharides typical of plant material. The major activities present being: mixed link glucanase, xylanase and both endo- and exo-cellulase. The straw treated with polysaccharidase had a lower final pH (5·21, 4·87, 4·82; P < 0·01), higher concentrations of lactic acid (1·98, 3·90, 3·67 g/kg; P < 0·01), a lower content of neutral-detergent fibre (689, 630, 621 g/kg DM; P < 0·01) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P < 0·01) for the 0, 5 and 10 g/kg polysaccharidase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of three sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p = a + b (1 – e−ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was increased (P < 0·01) by the addition of polysaccharidase. The maximum potential degradability (a + b), however, was not altered by any of the treatments. The values for c were 0·0498, 0·0677 and 0·0817 per h and for (a + b) were 62·4, 62·1 and 62·8 g/100 g DM for the 0, 5 and 10 g/kg polysaccharidase enzyme additions respectively. It is concluded that the use of polysaccharidase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

scholarly journals Long term conservation of DNA at ambient temperature. Implications for DNA data storage

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259868
Author(s):  
Delphine Coudy ◽  
Marthe Colotte ◽  
Aurélie Luis ◽  
Sophie Tuffet ◽  
Jacques Bonnet
Keyword(s):  
Data Storage ◽  
Room Temperature ◽  
Degradation Rate ◽  
White Blood Cells ◽  
High Capacity ◽  
Precise Determination ◽  
Buffy Coat ◽  
Degradation Rate Constant

DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under an anhydrous and anoxic atmosphere maintained inside hermetic capsules. The procedure has been validated by us and others for purified RNA, and for DNA in buffy coat or white blood cells lysates, but a precise determination of purified DNA stability is still lacking. We used the Arrhenius law to determine the DNA degradation rate at room temperature. We found that extrapolation to 25°C gave a degradation rate constant equivalent to about 1 cut/century/100 000 nucleotides, a stability several orders of magnitude larger than the current commercialized processes. Such a stability is fundamental for many applications such as the preservation of very large DNA molecules (particularly interesting in the context of genome sequencing) or oligonucleotides for DNA data storage. Capsules are also well suited for this latter application because of their high capacity. One can calculate that the 64 zettabytes of data produced in 2020 could be stored, standalone, for centuries, in about 20 kg of capsules.

scholarly journals Effect of physical form and washing of treated and untreated rice straw with sodium hydroxide upon chemical composition and in vitro digestibility

2008 ◽  
Vol 5 (2) ◽  
pp. 182-187
Author(s):  
Baghdad Science Journal
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Sodium Hydroxide ◽  
Rice Straw ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Physical Form

Hydroxide upon the chemical composition and dry matter(DM) and organic matter(OM) digestibility . Rice straw was treated with 4% sodium hydroxide using 30% of DM basis moisture, and incubated at 40 ºC for 40 days., DM digestibility (DMD) was significantly affected (P

scholarly journals Long term conservation of DNA at ambient temperature. Implications for DNA data storage

2021 ◽  
Author(s):  
Delphine Coudy ◽  
Marthe Colotte ◽  
Aurelie Luis ◽  
Sophie Tuffet ◽  
Jacques Bonnet
Keyword(s):  
Data Storage ◽  
Room Temperature ◽  
Degradation Rate ◽  
White Blood Cells ◽  
High Capacity ◽  
Precise Determination ◽  
Buffy Coat ◽  
Degradation Rate Constant

DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under an anhydrous and anoxic atmosphere maintained inside hermetic capsules. The procedure has been validated by us and others for purified RNA, and DNA in buffy coat or white blood cells lysates, but a precise determination of purified DNA stability is still lacking. We used the Arrhenius law to determine the DNA degradation rate at room temperature. We found that extrapolation to 25 degree C gave a degradation rate constant equivalent to about 1 cut per century per 100000 nucleotides, a stability several orders of magnitude larger than the current commercialized processes. Such a stability is fundamental for many applications such as the preservation of very large DNA molecules (particularly interesting in the context of genome sequencing) or oligonucleotides for DNA data storage. Capsules are also well suited for this latter application because of their high capacity. One can calculate that the 64 zettabytes of data produced in 2020 could be stored, standalone, for centuries, in about 20 kg of capsules.

scholarly journals STABILITY OF KETOPROFEN COATED BY CHITOSAN-GUAR GUM GEL

2010 ◽  
Vol 9 (3) ◽  
pp. 391-397
Author(s):  
Purwantiningsih Sugita ◽  
Bambang Srijanto ◽  
Budi Arifin ◽  
Ellin Vina Setyowati
Keyword(s):  
Room Temperature ◽  
Degradation Rate ◽  
Guar Gum ◽  
Reaction Kinetic ◽  
Tween 80 ◽  
Chitosan Solution ◽  
Climatic Chamber ◽  
Fine Powders ◽  
Magnetic Stirrer ◽  
Degradation Rate Constant

The coating stability of ketoprofen by chitosan-guar gum gel has been studied. Into 228.6 mL of 1.75% (w/v) chitosan solution in 1% (v/v) acetic acid, 38.1 mL of guar gum (gg) solution was added with concentration variation of 0.35, 0.55, and 0.75% (w/v) for ketoprofen microcapsules, and stirred with magnetic stirrer until homogenous. Afterwards, 7.62 mL of glutaraldehyde (glu) was added slowly under stirring, with concentrations varied: 3, 3.5, and 4% (v/v). All mixtures were shaked for 20 min for homogenization. Into each microcapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added. Every mixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. Every microcapsule formula was packed into capsules, as much as 100 g per capsule. The capsules were contained in 100-mL dark bottles and the bottles were kept in climatic chamber at (40 ± 2) °C and RH (75 ± 5) % for 3 months. The microcapsule stabilities were tested chemically and physically. The result showed that formulation of ketoprofen preparation composed of 1.75% (w/v) chitosan, 0.35% (w/v) gg, and 3.50% (v/v) glu, was relatively the best, with ketoprofen percentage left in microcapsule after 3 months, degradation rate constant, and shelf life of 80.33%, 0.0351 % week-1, and 18.92 months, respectively. Reaction kinetic model for this formula followed Prout-Tompkins equation and the degradation of ketoprofen was seem to follow autocatalytic reaction mechanism controlled by the formation and growth of reaction core.   Keywords: Ketoprofen, chitosan-guar gum gel

scholarly journals Synthesis of Magnetic Ferrocene-Containing Polymer with Photothermal Effects for Rapid Degradation of Methylene Blue

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 558
Author(s):  
Wenhui Zhu ◽  
Caiyun Zhang ◽  
Yali Chen ◽  
Qiliang Deng
Keyword(s):  
Methylene Blue ◽  
Photoelectron Spectroscopy ◽  
Room Temperature ◽  
Degradation Rate ◽  
Model Compound ◽  
Vibrating Sample Magnetometer ◽  
Simulated Sunlight ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ft Ir

Photothermal materials are attracting more and more attention. In this research, we synthesized a ferrocene-containing polymer with magnetism and photothermal properties. The resulting polymer was characterized by Fourier-transform infrared (FT-IR), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Its photo-thermocatalytic activity was investigated by choosing methylene blue (MB) as a model compound. The degradation percent of MB under an irradiated 808 nm laser reaches 99.5% within 15 min, and the degradation rate is 0.5517 min−1, which is 145 times more than that of room temperature degradation. Under irradiation with simulated sunlight, the degradation rate is 0.0092 min−1, which is approximately 2.5 times more than that of room temperature degradation. The present study may open up a feasible route to degrade organic pollutants.

A bioprinted composite hydrogel with controlled shear stress on cells

2020 ◽  
pp. 095441192097668
Author(s):  
Amirhossein Bakhtiiari ◽  
Rezvan Khorshidi ◽  
Fatemeh Yazdian ◽  
Hamid Rashedi ◽  
Meisam Omidi
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Room Temperature ◽  
Degradation Rate ◽  
Diffusion Rate ◽  
Sol Gel ◽  
Three Dimensional ◽  
Simulation Software ◽  
Sol Gel Transition ◽  
Gel Transition

In recent decades, three dimensional (3D) bio-printing technology has found widespread use in tissue engineering applications. The aim of this study is to scrutinize different parameters of the bioprinter – with the help of simulation software – to print a hydrogel so much so that avoid high amounts of shear stress which is detrimental for cell viability and cell proliferation. Rheology analysis was done on several hydrogels composed of different percentages of components: alginate, collagen, and gelatin. The results have led to the combination of percentages collagen:alginate:gelatin (1:4:8)% as the best condition which makes sol-gel transition at room temperature possible. The results have shown the highest diffusion rate and cell viability for the cross-linked sample with 1.5% CaCl2 for the duration of 1 h. Finally, we have succeeded in printing the hydrogel that is mechanically strong with suitable degradation rate and cell viability.

scholarly journals In situ degradability of dry matter and fibrous fraction of sorghum silage

2016 ◽  
Vol 38 (2) ◽  
pp. 171 ◽  
Author(s):  
Renê Ferreira Costa ◽  
Daniel Ananias de Assis Pires ◽  
Marielly Maria Almeida Moura ◽  
José Avelino Santos Rodrigues ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Degradation Rate ◽  
Degradation Kinetics ◽  
Grain Sorghum ◽  
Dual Purpose ◽  
Ruminal Degradability ◽  
Sorghum Silage ◽  
Kinetics Of

This study aimed to evaluate in situ degradability and degradation kinetics of DM, NDF and ADF of silage, with or without tannin in the grains. Two isogenic lines of grain sorghum (CMS-XS 114 with tannin and CMS-XS 165 without tannin) and two sorghum hybrids (BR-700 dual purpose with tannin and BR-601 forage without tannin) were ensiled; dried and ground silage samples were placed in nylon bags and introduced through the fistulas. After incubation for 6, 12, 24, 48, 72 and 96 hours, bags were taken for subsequent analysis of fibrous fractions. The experimental design was completely randomized with 4 replicates and 4 treatments and means compared by Tukey’s test at 5% probability. As for the DM degradation rate, silage of CMSXS165without tannin was superior. Silages of genotypes BR700 and CMSXS 114 with tannin showed the highest values of indigestible ADF (59.54 and 43.09%). Regarding the NDF, the potential degradation of silage of CMSXS165 line without tannin was superior. Tannin can reduce ruminal degradability of the dry matter and fibrous fractions. 

A note on propionic acid-treated barley of different moisture contents in the diets of growing pigs

1980 ◽  
Vol 31 (2) ◽  
pp. 213-215 ◽  
Author(s):  
D. J. A. Cole ◽  
I. H. Williams ◽  
P. R. English ◽  
J. R. Luscombe
Keyword(s):  
Propionic Acid ◽  
Acid Treatment ◽  
Dry Matter ◽  
Live Weight ◽  
Growing Pigs ◽  
Apparent Effect ◽  
Moisture Concentration ◽  
Moisture Contents ◽  
Physical Form ◽  
Conventional Manner

ABSTRACTGrowth and carcass traits were measured in pigs grown from 25 to 90 kg live weight on barley stored and prepared in different ways. Some of the barley was prepared in the conventional manner by drying to a moisture concentration of 140g/kg before hammer-milling. The remainder of the barley was rolled after treating batches, containing 140, 180 and 240 g moisture per kg, with propionic acid. A total of 128 pigs was used at three centres.There were no differences between the centres and no differences in the performance and carcass measurements of pigs given acid-treated and rolled, or untreated and milled barley, despite differences in physical form between the rolled and milled samples. When the intake of dry matter was equalized there was no apparent effect on the pigs of acid treatment of barley containing different amounts of moisture.

Effects of nutrition in late pregnancy on subsequent milk production in Ewes

1970 ◽  
Vol 12 (1) ◽  
pp. 23-36 ◽  
Author(s):  
T. T. Treacher
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Live Weight ◽  
Late Pregnancy ◽  
Lower Content ◽  
Weight Changes ◽  
Total Birth ◽  
Weight Gains ◽  
Ad Libitum ◽  
Voluntary Intake

SUMMARY1. Scottish Half-bred ewes carrying twin foetuses were fed individually to make live-weight gains in the last six weeks of pregnancy of (1) 20%, (2) 10% and (3) 0% of their live weight in week 14 of pregnancy. In lactation the ewes were fed ad libitum. The lambs were removed 12 to 16 hr after parturition and the ewes were machine-milked twice daily for the first six weeks of lactation.2. Total birth weights per ewe of twin lambs from the treatments were (1) 10·10 kg, (2) 9·44 kg and (3) 8·18 kg and differed significantly.3. The level and pattern of voluntary intake in lactation did not differ significantly between the treatments. Total dry-matter intakes in the six weeks of lactation were (1) 121·9 kg (2) 105·9 kg and (3) 109·5 kg.4. The pregnancy treatments affected the level of milk production and the shape of lactation curves. The total yields in the first six weeks of lactation were (1) 58·8 kg, (2) 43·5 kg and (3) 26·9 kg. Higher contents of fat and protein and the lower content of lactose in the milk from treatment-3 ewes on days 1 and 3 of lactation indicated a slower onset of lactation in these ewes. Between days 7 and 35 of lactation the contents of fat and SNF were lowest on treatment 3 but the differences were not significant.5. The live-weight changes in lactation, which were in inverse order to the gains in late pregnancy, were (1) 3·4 kg, (2) 5·5 kg and (3) 9·5 kg.

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