Effect of growth phase on harvesting characteristics, autoflocculation and lipid content of Ettlia texensis for microalgal biodiesel production

2013 ◽  
Vol 138 ◽  
pp. 214-221 ◽  
Author(s):  
S. Salim ◽  
Z. Shi ◽  
M.H. Vermuë ◽  
R.H. Wijffels
Keyword(s):  
Lipid Content ◽  
Growth Phase ◽  
Biodiesel Production ◽  
Microalgal Biodiesel

Mixotrophic Cultivation of Scenedesmus sp. as Biodiesel Feedstock

2013 ◽  
Vol 777 ◽  
pp. 268-273 ◽  
Author(s):  
Jing Han Wang ◽  
Hai Zhen Yang ◽  
Feng Wang
Keyword(s):  
Lipid Content ◽  
Synergetic Effect ◽  
Biomass Productivity ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Heterotrophic Culture ◽  
Glucose Addition ◽  
Biodiesel Feedstock ◽  
Microalgal Biodiesel

Microalgae are a promising feedstock for biodiesel production. Microalgal biodiesel can be obtained under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Reported studies of microalgal biodiesel production are mainly based on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity of Scenedesmus sp. under photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was added as organic carbon source at five concentrations (0.1, 0.5, 1.0, 2.0, 5.0% glucose w/v). Results displayed that microalgal growth was significantly improved in glucose supplied cultures. Synergetic effect of photoautotrophy and heterotrophy existed in all mixotrophic cultures. Highest biomass productivity of 1.307 g·L-1·d-1 and highest lipid productivity of 316 mg·L-1·d-1 was respectively observed under mixotrophic cultivation with 5.0% and 1.0% (w/v) glucose addition. Lipid content of Scenedesmus sp. under mixotrophic cultivation was mostly higher in stationary phase than in exponential phase. Highest lipid content of 27.73% was observed in 1.0% mixotrophic culture, followed by 24.66% in 1.0% heterotrophic culture.

Potential of Mixotrophic Cultivation of Chlorella sorokiniana for Biodiesel Production

2013 ◽  
Vol 779-780 ◽  
pp. 1509-1513
Author(s):  
Jing Han Wang ◽  
Hai Zhen Yang ◽  
Feng Wang
Keyword(s):  
Lipid Content ◽  
Synergetic Effect ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Mixotrophic Cultivation ◽  
Promising Alternative ◽  
Microalgal Biodiesel

Biodiesel from microalgae provides a promising alternative for biofuel production. Microalgal biodiesel can be produced under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Studies of biodiesel production from microalgae have been reported mostly focusing on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity ofChlorella sorokinianaunder photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was adopted as organic carbon source at five concentrations (0.1, 0.5, 1.0, 2.0, 5.0% glucose w/v). Results displayed that microalgal growth was significantly improved in glucose supplied cultures. Synergetic effect of photoautotrophy and heterotrophy existed in mixotrophic cultivation except for 5.0% culture. Highest biomass productivity of 1.178 g·L-1·d-1and highest lipid productivity of 582 mg·L-1·d-1was observed under mixotrophic cultivation with 2.0% (w/v) glucose addition. Lipid content ofC. sorokinianawas mostly higher in stationary phase than in exponential phase. Highest lipid content of 49.37% was observed in 2.0% mixotrophic culture, followed by 47.09% in 2.0% heterotrophic culture.

scholarly journals PENGARUH PERBEDAAN UMUR PANEN TERHADAP KANDUNGAN LEMAK Nitzschia sp.

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Baiq Tri Khairina Ilhami, Lalu Japa Sri Puji Astuti dan Rina Kurnianingsih
Keyword(s):  
Lipid Content ◽  
Light Energy ◽  
Raw Material ◽  
Biodiesel Production ◽  
Wide Area ◽  
Experiment Research ◽  
Soxhlet Method ◽  
Sun Light

ABSTRAK Mikroalga merupakan protista berklorofil yang memanfaatkan energi matahari dan CO2 dalam proses fotosintesis sehingga dalam biomassanya terkandung bahan-bahan seperti: lemak, protein, dan karbohidrat. Salah satu jenis mikroalga yang memiliki kandungan lemak adalah Nitzschia sp. Kelebihan mikroalga sebagai bahan baku biodiesel jika dibandingkan dengan jarak, biji bunga matahari, jagung, dan tumbuhan lainnya yaitu pertumbuhan mikroalga yang sangat cepat, tidak bersaing dengan produksi pangan, tidak membutuhkan lahan yang luas, dan ramah lingkungan. Oleh karena itu, perlu dilakukan penelitian untuk mengetahui kandungan lemak tertinggi pada Nitzschia sp. berdasarkan umur kultivasi. Dilakukan penelitian eksperimental kuantitatif menggunakan  Rancangan Acak Lengkap (RAL) dengan 5  kali panen dan 3 kali ulangan, percobaan dilakukan di Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong pada bulan Agustus sampai September 2014. Kadar lemak ditentukan dengan Metode Soxhlet. Hasil penelitian menunjukkan umur pemanenan hari ke-13 memiliki kandungan lemak tertinggi dengan persentase sebesar 1,84% sedangkan kandungan lemak terendah pada umur panen hari ke-11 dengan persentase sebesar 0,89%. Tinggi rendahnya kandungan lemak Nitzschia sp. dipengaruhi oleh nutrien, kepadatan sel dan faktor lingkungan media kultur.  ABSTRACT Photosynthetic Protist, microalgae can trap sun light energy and CO2 for photosynthesis process, and they produce lipid, protein, and carbohydrate. One species of  microalga that can produce lipid in high portion of their total dry wight is Nitzschia sp. The lipid can highly use as raw material for biodiesel production. The benefits of  microalga as source of biodiesel are they grow faster, they do not compete with other food soruces production, they do not need wide area, and they are invironmental friendly. A quantitative experiment research of rendom complete design was done in the Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong during the months of August to  September 2014. The research was conducted in five time harfesting of each in three repetation The lipid content was ditermined using Soxhlet method. The results showing that the highest lipid content (1.84%) of Nitzschia sp. occurred at the day of thirteen of culturing, and the lowest lipid content (0.89%) of Nitzschia sp. was in the day of eleven of culture. 

Optimization of lipid accumulation in Pleurastrum insigne for biodiesel production

2021 ◽  
Vol 16 (10) ◽  
pp. 144-155
Author(s):  
Van Lal Michael Chhandama ◽  
Belur Kumudini Satyan
Keyword(s):  
Lipid Content ◽  
Lipid Production ◽  
Statistical Design ◽  
High Growth ◽  
High Growth Rate ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Biodiesel Standards ◽  
High Lipid ◽  
Different Sources

Microalgae emerged as a competent feedstock for biodiesel production because of high growth rate and lipid content. This work focuses on isolation of novel microalgal strain from different sources of water for the production of biodiesel. The isolated microalgae, Pleurastrum insigne possessed high lipid content (~28 % dcw), further optimized to 57.06 % dcw using a statistical design (CCD) under Response Surface Methodology. Lipid production was optimized by nutrient (nitrogen and phosphorus) and pH stress. The different type of fatty acids present in the optimized lipid was also profiled using GCMS. Biodiesel yield was found to be 82.14 % of the total lipid and the fuel properties tested have met IS, ASTM and EN biodiesel standards.

scholarly journals Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Martani ◽  
Letizia Maestroni ◽  
Mattia Torchio ◽  
Diletta Ami ◽  
Antonino Natalello ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Lipid Content ◽  
Lipid Production ◽  
Batch Process ◽  
Batch Cultivation ◽  
Biodiesel Production ◽  
Cellular Lipid ◽  
Lipomyces Starkeyi ◽  
Yeast Cultures ◽  
Beet Pulp

Abstract Background Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required. Results In this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2%, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2%) in the presence of 6% molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2%, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. Conclusions This study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor. Graphical abstract

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